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Citations for Thermo Fisher :
3601 - 3650 of 7738 citations for Recombinant Human TNFRSF1B His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... The following additional niche factors were added to generate “Full” media: 50 ng/ml mouse recombinant EGF (Life Technologies), 100 ng/ml mouse recombinant Noggin (Peprotech) ...
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bioRxiv - Cancer Biology 2020Quote: ... 293T cells were transfected with recombinant lentiCRISPRv2 together with psPAX2 and pMD2.G using Lipofectamine 2000 (Thermo Fisher Scientific). Undigested lentiCRISPRv2 plasmid lacking sgRNA sequence was used for pseudovirus production as a control ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-2P trimer was biotinylated using the EZ-Link Sulfo-NHS-LC Biotinylation Kit (ThermoFisher) and tetramerized with streptavidin-APC (Agilent ...
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bioRxiv - Cell Biology 2020Quote: ... the nucleotide sequence was inserted by means of a BP recombinant reaction (Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA) according to the manufacturer’s protocol into a pDONR221 donor vector ...
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bioRxiv - Microbiology 2020Quote: ... at day 0 the animals were subcutaneously administered either approximately 10,000,000 fffus of detergent-inactivated UHV or 0.1 mg of recombinant UHV NP emulsified in Freund’s incomplete adjuvant (ThermoFisher Scientific), the total volume per individual was 125 μl ...
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bioRxiv - Microbiology 2021Quote: Cells were transfected with recombinant plasmid p3xFLAG-NS5A or NS5A mutant expression vectors using Lipo3000 (Thermo Fisher Scientific, USA) according to the manufacturer’s directions ...
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bioRxiv - Genomics 2021Quote: Total RNA was extracted using the hot-phenol method (Schmitt et al., 1990) and treated with recombinant DnaseI (Ambion). For real-time reverse transcription PCR ...
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bioRxiv - Bioengineering 2022Quote: ... 4 μL of capture Ab (~4 μg/ml) or SARS-CoV-2 recombinant antigen (~0.2 mg/ml) in 1x PBS (Gibco) was loaded in each channel ...
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bioRxiv - Immunology 2022Quote: ... recombinant GBPs were incubated with Alexa Fluor 488 C5 maleimide dye or Alexa Fluor 647 C2 maleimide dye (Invitrogen) in a ratio of 1:1 or 1:2 on ice for 10–20 min ...
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bioRxiv - Microbiology 2022Quote: recombinant biotinylated sE-cvD protein was expressed at 28°C using an ExpiFectamine 293 transfection kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Neuroscience 2023Quote: Sf9 cells for generation of baculovirus and expression of recombinant antibody fragment are from Thermo Fisher (12659017, lot 421973). The cells were not authenticated experimentally for these studies ...
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bioRxiv - Physiology 2023Quote: A recombinant adenovirus expressing mouse Sult2b1 (Ad-Sult2b1) was produced using the ViraPower Adenoviral Expression System (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Endpoint PCR for all mouse CASK experiments was performed using 0.25 U recombinant Taq DNA Polymerase (Thermo Fisher Scientific) with 2 mM MgCl2 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Batches of ten 8-10 weeks old female BALB/c mice were inoculated intraperitoneally (i.p) with 105 PFU of Addavax-adjuvant recombinant virus in 200 μL of phosphate-buffered saline (PBS; Gibco). At twenty-one days post immunization ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant proteins were purified by immobilized metal affinity chromatography (IMAC) as previously described30 using HisPur cobalt resin (Thermo Scientific). Before elution ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with the recombinant pcDNA3.1 plasmid (6 μg/flask) containing mouse Wnt10b cDNA using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Purity of recombinant proteins was verified by SDS-PAGE with staining by GelCode Blue stain reagent (Thermo Fisher scientific).
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bioRxiv - Biophysics 2024Quote: ... supplemented with 100 units/mL recombinant IL-2 IS (130- 097-744, Miltenyi) and 11% horse serum (26050088, Gibco). Jurkat T cells (TIB-152 ...
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bioRxiv - Neuroscience 2024Quote: ... A polyclonal antibody to Drosophila tau was prepared in rabbits immunized with full length recombinant tau protein (Thermo Fisher) and was used at 1:5,000,000 for western blotting ...
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bioRxiv - Microbiology 2024Quote: Recombinant soluble spike and RBD proteins from Wuhan-1 and BA.1 SARS-CoV-2 strains were expressed as described 58,59 Recombinant proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: All CoMiX were generated from HEK293T cells transfected with four μg of recombinant plasmid DNA using Lipofectamine-3000 (Invitrogen, Thermo Fisher Scientific BVBA ...
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bioRxiv - Biophysics 2023Quote: ... Recombinant bacmids were generated from the plasmids in Table S1 using MAX Efficiency DH10Bac competent cells (Gibco; Cat. 10361012) and transfected into Sf9 insect cells (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: The recombinant abGFP4 nanobody against GFP with a C-terminal cysteine was covalently coupled to SulfoLink resin (Thermo Scientific) consisting of cross-linked agarose beads with iodoacetyl groups ...
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bioRxiv - Cell Biology 2023Quote: ... Moloney murine leukemia virus (M-MLV) reverse transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor were purchased from Invitrogen (Carlsbad, CA). The Phospho-Akt (Ser473 ...
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bioRxiv - Plant Biology 2023Quote: ... Preparation of recombinant bacmid DNA and transfection of Spodoptera frugiperda 9 (Sf9) cells were performed according to the manufacturer’s instructions (Invitrogen). SbCYP711A31 was expressed in Sf9 cells and microsomal fractions of the insect cells expressing SbCYP711A31 were obtained as described previously58 ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates were incubated in shake tubes over 1 h at 4°C with recombinant protein G–Sepharose (Invitrogen). The Sepharose was washed with RIPA lysis buffer and eluates were obtained through an addition of Laemmli Buffer.
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bioRxiv - Immunology 2023Quote: ... Ganglioside M1 (GM1) staining was performed using recombinant Choleratoxin B labelled with AlexaFluor647 (3 µg/ml, Thermo Fisher, #C34778). Both GFP-D4 and AlexaFluor647-labelled CTB were added to FACS buffer-washed cells and incubated for 20 minutes at 4°C in the dark ...
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bioRxiv - Biochemistry 2022Quote: ... The pKL plasmid was used for the generation of recombinant Baculoviruses using the Bac-to-Bac expression system (Invitrogen).
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bioRxiv - Neuroscience 2023Quote: Four sections from each mouse were stained using primary antibodies calbindin D28K anti-rabbit recombinant polyclonal (1:200; ThermoFisher) and parvalbumin anti-mouse monoclonal (1:200 ...
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bioRxiv - Biochemistry 2023Quote: ... The coding sequences of the recombinant proteins were then inserted into the BamHI and EcoRI sites of the pcDNA3.1(+) vector (Invitrogen), and all clones were verified through DNA sequencing.
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bioRxiv - Microbiology 2024Quote: Recombinant Bd37 (rBd37) protein was expressed as thioredoxin fusion protein from the pBAD/Thio-TOPO vector (Thermo Fisher Scientific), which encodes thioredoxin and histidine-tag at N-terminus and C-terminus of multi cloning site ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant lentivirus used to infect SW620 cells for protein expression was generated by the ViraPower Lentiviral Expression System (Invitrogen) according to the manufacturer’s instruction.
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bioRxiv - Immunology 2024Quote: ... GPI-RBD-hGM-CSF recombinant fusion protein and GPI-hIL-12 were affinity purified from CHO-S cells (Invitrogen) stably expressing the proteins using anti-human GM-CSF (clone BVD2-21C11 ...
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bioRxiv - Microbiology 2024Quote: ... the sequence of the resulting recombinant plasmid was ascertained by Sanger sequencing techniques (Applied Biosystems™ 3130 Genetic Analyser), and the resulting data were decoded by ApE software (v2.0.36 ...
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bioRxiv - Biochemistry 2020Quote: ... Cytosolic extracts were generated as described above and the His6-tagged proteins purified by metal affinity chromatography and gravity flow using 0.5 ml HisPur™ cobalt superflow agarose (Thermo Fisher Scientific™, Waltham, USA) according to manufacturer’s protocol ...
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bioRxiv - Biophysics 2019Quote: ... The purity of affinity purified histidine tagged CsgF was investigated using SDS PAGE and the presence of the histidine tag was confirmed by western blot using an Anti-His (C-Term)-HRP antibody (Life Technologies, Carlsbad, CA).
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bioRxiv - Molecular Biology 2021Quote: ... enrichment and chip loading was performed on an Ion Proton Chef system using the Ion PI™ Hi-Q™ Chef Kits (ThermoFisher Scientific); sequencing was performed on an Ion Proton PI™ V3 chip ...
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bioRxiv - Immunology 2022Quote: ... Human PBMCs and C57BL/6 mouse PBMCs not immediately used in any experiments were stored down by resuspending cells in freezing media (HI-FBS with 10% DMSO, Fisher Scientific, BP231-100) in aliquots of 5-10 x106 cells per ml and frozen down to -80°C at a speed of 1°C/min prior to storage in liquid nitrogen.
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bioRxiv - Immunology 2022Quote: ... Quantitative real-time PCR was performed by using qPCRBIO Syber Green Mix Hi-ROX (PCR Biosystems, Wayne, US) and run with the StepOne Real-Time PCR System (Applied Biosystems, Waltham, US). The relative gene expression was calculated as 1/2ΔCT based on the normalization by the reference gene Hprt1 ...
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bioRxiv - Cell Biology 2020Quote: ... purified His-calmodulin was dialyzed in a Spectra/Por™ 4 RC Dialysis Membrane Tubing (08-667D, Thermo Fisher Scientific, Waltham, MA, USA) against 1,000 volumes of PBS supplemented with 0.5 mM EGTA at 4 °C for 4 h and replaced with fresh 1,000 volumes of PBS twice.
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bioRxiv - Microbiology 2020Quote: ... and blots were incubated for 1 h with a 1:1,000 dilution of anti 6X-His Tag rabbit antibodies (Thermo Fisher Scientific, Massachusetts, USA) followed by a 1 h incubation with a 1:3,000 dilution of goat anti-rabbit IgG coupled to HRP secondary antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... Dm III-2 and Dm III-2 + cys production was confirmed via western blot using 6x His-tag Ab (Invitrogen, Carlsbad, CA, USA). Positive clones were expanded ...
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bioRxiv - Molecular Biology 2024Quote: ... were amplified from differentiated P19 mouse embryonal carcinoma cells15 and ligated into the multiple cloning site of the expression vector pcDNA3.1 myc-His B (Thermo Fisher Scientific; Waltham, MA). Snapin was cloned in-frame with the downstream myc-His tags ...
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bioRxiv - Genetics 2024Quote: ... Membrane was stripped using PVDF stripping solution (DOT Scientific 65000-500) according to manufacturer instructions and reprobed with anti-6x-His primary antibody (Thermo Fisher MA1-21315) and conducted identically to the first probing ...
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bioRxiv - Neuroscience 2023Quote: An PPP2R2B-AS1 fragment containing the region 44 bp upstream of the CTG repeat and 145 bp downstream from the repeat was PCR-amplified and cloned into pcDNA3.1(-) myc-his A vector (Life Technologies, Grand Island, NY) at EcoRV site ...
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bioRxiv - Immunology 2023Quote: ... The resulting libraries were multiplexed and sequenced with Ion Proton technology and Ion Hi-Q chemistry (Ion Torrent, Thermo Fisher Scientific, Vienna, Austria). CUTADAPT 4.0 was used to trim sequencing reads of low-quality bases and poly-A tails with the options -q 20 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the pull-down assay follows the protocol provided by Pierce™ His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, Waltham, MA). The fluorescence intensity (excitation = 485 ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was probed with primary antibodies including anti-tau mouse ET3 (1:1000 dilution) and 6X - His Tag rabbit monoclonal antibody (Invitrogen, 1:3500 dilution). The secondary antibodies used were ...
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bioRxiv - Cell Biology 2023Quote: Primary antibodies used in western blotting (WB) and immunofluorescence (IF) were anti-6x His mouse monoclonal (Invitrogen, #MA1-21315, 1:5000 in WB), anti-GST mouse monoclonal (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... to digest the phosphorylated OsPrx20-His and analyzed by the LC-MS/MS system (LTQ Orbitrap XL ETD; ThermoFisher Scientific, Waltham, MA, USA) as described previously (Gampala et al. ...