Labshake search
Citations for Thermo Fisher :
3601 - 3650 of 10000+ citations for Recombinant Goat VEGFA Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor conjugated secondary antibodies (Alexa Fluor 488 conjugated goat anti-guinea pig, and goat anti-rabbit were from Invitrogen, MA, USA) were used at 1:500.
-
bioRxiv - Cell Biology 2023Quote: ... Cells were then washed with PBS and incubated with secondary antibodies Goat anti-Mouse IgG (H+L) Alexa Fluor™ 594 and Goat anti-Rabbit IgG (H+L) Alexa Fluor™ 488 (Invitrogen) for one hour at room temperature ...
-
bioRxiv - Bioengineering 2023Quote: ... BLG-specific IgE Abs were detected with goat anti-mouse IgE-unlabeled (Southern Biotechnology Associates, Birmingham, AL) and rabbit anti-goat IgG-alkaline phosphatase (Invitrogen, Eugene, Oregon) and developed with p-nitrophenyl phosphate “PNPP” (SeraCare Life Sciences ...
-
bioRxiv - Neuroscience 2024Quote: ... neurons were washed with PBS three times for 5 min and incubated with secondary antibodies (goat anti-rabbit-AlexaFluor488: 1:1000, Thermo Fisher Scientific, Cat. #A-11034; goat anti-mouse-AlexaFluor488: 1:1000, Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2023Quote: ... the embryos were stained with secondary antibodies with Alexa 488 goat anti-rat IgG and Alexa 594 goat-anti-mouse IgG (1:250, Invitrogen, Carlsbad, CA) in blocking buffer overnight ...
-
bioRxiv - Neuroscience 2023Quote: ... Membranes were then washed three times in TBS-T and incubated for 1 h at RT with goat anti-rabbit or goat anti-mouse HRP conjugated secondary antibodies (31460 and 31430, respectively, Thermo Fisher Scientific) diluted 1:10000 in TBS-T ...
-
bioRxiv - Immunology 2023Quote: ... Cells were then stained with fluorescent secondary antibodies (goat anti-rabbit Alexa Fluor 488, and/or goat anti-mouse Alexa Fluor 488; Invitrogen, 1:1,000), and finally washed with PBS three times for 5 min each ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were then washed with PBS with 0.03% NaN3 for 15 mins RT × 4 and incubated in secondary antibodies at 1:1000 dilution (goat anti-mouse 488, Thermo Fisher Scientific# A-28175, goat anti-rabbit 647, Thermo Fisher Scientific# A-27040 ...
-
bioRxiv - Cancer Biology 2023Quote: ... or 53BP1 clone 6B3E10 (Santa-Cruz) and visualized using Alexafluor-488-conjugated goat anti-rabbit and Alexfluor-546-conjugated goat anti-mouse antibodies (Invitrogen™, Life technologies) along with 4′,6-diamidino-2-phenylindole ...
-
bioRxiv - Developmental Biology 2024Quote: ... Jackson,711-545-152), donkey anti-goat-555 (1:250, A-21432) or donkey anti-goat 647 (1:250, Invitrogen, A-21447). For GREM1 protein and pSMAD1.5.9 ...
-
bioRxiv - Systems Biology 2024Quote: ... Cells were washed and incubated with either an AF488-conjugated goat anti-rabbit or an AF488-conjugated goat anti-mouse secondary antibody as appropriate (1:300, Thermo-Fisher Scientific, UK) in 1 % FCS ...
-
bioRxiv - Molecular Biology 2024Quote: ... re-probed for 1 hour with 1:5000 HRP-conjugated secondary antibodies (donkey anti-goat, Santa Cruz #sc-2020; goat anti-rabbit, Invitrogen #31462), washed three times for 5 min with TBST ...
-
bioRxiv - Microbiology 2024Quote: ... Alexa Fluor 488- or 594-conjugated goat anti-mouse IgG and Alexa Fluor 488- or 594-conjugated goat anti-rabbit IgG (1:200; Thermo Fisher Scientific) were reacted at 37°C for 1 h ...
-
bioRxiv - Neuroscience 2024Quote: ... membranes were then incubated in appropriate secondary antibodies (goat anti-rabbit-HRP, Invitrogen G21234, 1:10,000; goat anti-mouse IgG1, Invitrogen PA174421, 1:5,000) for 30 minutes at room temperature before washing and enhanced chemiluminescence with (SuperSignal™ West Femto Maximum Sensitivity Substrate ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations were determined with the Pierce BCA Protein Assay Kit (Thermo Scientific), and extracts were loaded onto NuPAGE Bis–Tris SDS gels and immunoblots were visualized by LiCOR Odyssey system.
-
bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific), and the pHrodo-TDM1 fluorescent signal was normalized to the amount of protein at each time point.
-
bioRxiv - Cancer Biology 2021Quote: ... The total protein concentrations were determined using BCA protein assay kit (Thermo Scientific), and IFN-α and IFN-γ protein concentrations were measured using a human IFN-α ELISA kit (1110012 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was determined using Pierce BCA protein assay kit (Thermo Fisher Scientific) and protein lysates were subjected to electrophoresis through SDS–PAGE or 4-12% NuPage Bis-Tris protein gels (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... protein concentration of the supernatant was determined using BCA protein assay (Thermo Fisher) and 50 μg (1C and S2F ...
-
bioRxiv - Neuroscience 2020Quote: ... Total protein content was quantified using a BCA Protein Assay Kit (Thermo Scientific) and equal amounts of total protein were loaded onto 4 – 15% Tris-Glycine gels (BioRad) ...
-
bioRxiv - Cell Biology 2019Quote: ... and protein concentrations were determined using the BCA protein assay (Thermo Fisher Scientific). Protein lysates were analyzed by SDS PAGE and Western blot using fluorescently-labeled secondary antibodies ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized using a reversible protein stain (Memcode, Pierce, Thermo Fisher Scientific) and membranes were incubated with the following primary antibodies listed in Table S3 ...
-
bioRxiv - Cell Biology 2020Quote: ... The total protein content was measured using BCA Protein Assay Kit (ThermoFisher Scientific). The relative ALP activity was normalized to total protein content and expressed as units/g·protein.
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentration was measured using the Pierce BCA Protein Assay kit (Thermo Fisher). An equal volume of 2x Laemmli buffer (BIO-RAD ...
-
bioRxiv - Cell Biology 2020Quote: ... and protein concentration was determined with the Pierce BCA protein assay kit (ThermoFisher). Insoluble materials were removed by centrifugation at 21,000 g for 30 mins at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using a BCA protein assay kit (Thermo Fisher Scientific). Aliquots of lysates were solubilized in Laemmli sample buffer and equal amounts of proteins were separated on 12% SDS-PAGE gels ...
-
bioRxiv - Immunology 2021Quote: ... Protein concentration was quantified using Pierce BCA protein assay kit (Thermo Fisher Scientific). Tissue lysates were mixed with 2x Laemmli sample buffer and incubated at 100°C for 5 min and equal amounts of protein were loaded into a 4-15% polyacrylamide gel for SDS/PAGE and transferred onto a nitrocellulose membrane (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Sample protein concentrations were determined using the BCA Protein Assay Kit (Thermo Scientific). Primary antibodies were anti-Six1 (rabbit polyclonal HPA001893 ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Extracted proteins were quantified using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) and stored at −80°C until further analysis.
-
bioRxiv - Immunology 2020Quote: ... Protein concentrations were determined using the BCA protein assay kit (Thermo Scientific #A53225). Protein samples were separated by SDS–PAGE (Invitrogen #NW04125BOX) ...
-
bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were determined using Pierce™ Coomassie (Bradford) Protein Assay Kit (ThermoFisher). Protein sample loading buffer (62.5 mM Tris-Cl pH6.8 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Protein was quantified using Pierce™ 660 nm Protein Assay Reagent (Thermo Scientific) and 1 mg (for adults ...
-
bioRxiv - Developmental Biology 2020Quote: ... Protein concentrations were measured using a Pierce BCA Protein Assay Kit (Thermo Fisher), and 50 µg of denatured proteins were run on a 12% acrylamide gel and transferred to a 0.2 µm nitrocellulose membrane (BioRad ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein concentration was determined using BCA protein assay kit (Thermo Scientific, Rockford, IL).
-
bioRxiv - Neuroscience 2021Quote: ... Protein concentration was determined with the BCA protein assay kit (Thermo Fisher 23227) using a bovine serum albumin (BSA ...
-
bioRxiv - Neuroscience 2020Quote: ... AHA-conjugated proteins were biotinylated using the Protein Reaction Buffer kit (Thermo Fisher), following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... the protein bands were visualized by staining with Imperial Protein Stain (Thermo Scientific) for 1 hr followed by overnight de-staining with Milli-Q water ...
-
bioRxiv - Molecular Biology 2020Quote: ... The protein bands were visualized by staining with Imperial Protein Stain (Thermo Scientific) for 1 hr followed by overnight de-staining with Milli-Q water ...
-
bioRxiv - Molecular Biology 2020Quote: ... Protein concentration was determined with a Pierce BCA Protein Assay Kit (Thermo Scientific) and all samples were stored at -80°C until further use.
-
bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were measured using bicinchoninic acid (BCA) protein assay (Thermo Fisher Scientific). Ten to 30 µg of protein samples were loaded on 10% sodium dodecyl sulfate polyacrylamide (SDS-PAGE ...
-
bioRxiv - Physiology 2021Quote: ... Protein was measured using a Pierce™ BCA protein assay kit (Thermo Fisher) and mixed 1:1 with 2x Laemmli buffer (Bio-Rad ...
-
Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay (Thermo Fisher Scientific). 30 μg of protein mixed with loading buffer (62.5 mM Tris pH 6.8 ...
-
bioRxiv - Cell Biology 2022Quote: ... and lysate protein concentrations were measured using Qubit protein assay kit (ThermoFisher Scientific). For Western blots ...
-
bioRxiv - Microbiology 2020Quote: ... Protein estimation was done using Pierce BCA Protein Assay kit (Thermo Fisher Scientific). 5 μg protein lysate sample of each time point was prepared using SDS sample buffer containing 250 mM Tris-HCl (pH 6.8) ...
-
bioRxiv - Microbiology 2020Quote: ... Protein estimation was done using Pierce BCA Protein Assay kit (Thermo Fisher Scientific).
-
bioRxiv - Microbiology 2020Quote: ... Protein concentrations were detected using a BCA protein quantification kit (Thermo Fisher Scientific). Equal amounts of protein were separated by 10% SDS-PAGE ...
-
bioRxiv - Cancer Biology 2021Quote: ... The total protein concentration was determined by Pierce Protein assay (Thermo Fisher Scientific). Protein samples (5μg ...
-
bioRxiv - Immunology 2021Quote: ... protein concentrations were determined with the Pierce BCA Protein Assay Kit (Thermo Scientific). CCL5 concentration in the tumor lysates was analyzed by ELISA (Mouse RANTES Uncoated ELISA Kit Invitrogen ...
-
bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were estimated using Pierce BCA protein assay kit (Thermo scientific #23225) as per manufactural instructions and equal concentration of proteins resolved appropriate SDS PAGE gels ...
-
bioRxiv - Cell Biology 2019Quote: ... Proteins were quantitated using bicinchoninic acid (BCA) protein assay (Pierce/Thermo Scientific, 23225) per manufacturer’s instruction ...