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Citations for Thermo Fisher :
3601 - 3650 of 5129 citations for JIP3 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Sections were soaked in PBS for 10 minutes before incubation in blocking solution (20% fetal calf serum (FCS, Thermo Fisher Scientific), and 20% normal goat serum (NGS ...
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bioRxiv - Physiology 2022Quote: ... Cells were then washed with PBS-S-T and incubated for 1h at 37°C with the following secondary antibodies diluted in blocking buffer: goat anti-rat Alexa Fluor 488 (1:500, catalog no. A-11006, ThermoFisher Scientific), goat anti-rabbit Alexa Fluor 594 (1:500 ...
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bioRxiv - Physiology 2022Quote: ... Slides were washed three times for 5 min with TBS + 0.1% Tween 20 and then incubated for 1h at RT with the following secondary antibodies diluted in blocking buffer: goat anti-rat Alexa Fluor 488 (1:500, catalog no. A-11006, ThermoFisher Scientific), goat anti-rabbit Alexa Fluor 594 (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed with PBS three times and incubated in the blocking buffer containing anti-mouse IgG donkey antibody conjugated with Alexa 488 (1:500 dilution, Thermo Fisher), anti-rabbit IgG donkey antibody conjugated with Alexa 555 (1:500 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 μL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Genetics 2022Quote: ... samples were blocked in RNase free blocking solution (0.3% v/v PBT, 0.5% ultra-pure Bovine Serum Albumin) (Ambion, cat. no. AM2616) for 45 min and primary and secondary antibody stainings were carried out ...
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bioRxiv - Neuroscience 2022Quote: ... The secondary antibodies were Alexa Fluor 488 goat anti-mouse (green) and Alexa Fluor 568 goat anti-rat (red) (used at 1:250 dilution in blocking solution, Life Technologies).
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bioRxiv - Microbiology 2022Quote: ... diluted 1:200 in blocking buffer for 30 min and mounted with ProLong Gold Antifade with DAPI (4′,6-diamidino-2- phenylindole; Thermo Fisher). Confocal laser micrographs were obtained with Zeiss LSM 880 laser microscope and analyzed with Zen black and Fiji software ...
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bioRxiv - Neuroscience 2020Quote: ... This was followed by secondary antibody staining with Alexa-Fluor 488 (1:500 dilution prepared in blocking buffer, incubated for 1 hour at room temperature) (A-11008, ThermoFisher Scientific) to visualize MAP2 ...
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bioRxiv - Neuroscience 2019Quote: ... which were boiled for 5 min in PBS and subsequently incubated with the blocking solution containing 0.2% I-Block (Thermo Fisher Scientific) and 0.1% Tween 20 (Merck ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then washed 3 x 15 min in WB at RT and secondaries were then diluted in BB blocking buffer (Donkey anti rabbit AF568 (Invitrogen #A10042) 1:750 ...
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bioRxiv - Cell Biology 2020Quote: On day 2 of cardiac differentiation cells were dissociated using 0.5% EDTA and put into blocking buffer of 50% fetal bovine serum (FBS) in Dulbecco’s Modified Eagle Medium (DMEM)/F12 (ThermoFisher, Cat.#11320033). Cells were then pelleted and resuspended in 10% FBS in DMEM media ...
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bioRxiv - Immunology 2020Quote: ... were diluted in LI-COR blocking buffer and detected using goat anti-rabbit-AF680 and goat anti-mouse-AF790 secondary reagents (Molecular Probes). Protein bands were detected and quantified using a Li-COR Odyssey Imaging System.
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bioRxiv - Biochemistry 2021Quote: ... 10 μg ml−1 diluted in 2% goat serum- and 2 mM EDTA-containing D-PBS (blocking buffer)) and a goat anti-mouse IgG secondary antibody conjugated with Alexa Fluor 488 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The glial marker antibodies were all diluted 1:200 in blocking buffer and detected with donkey anti-rabbit IgG conjugated with Alexa Fluor 594 (1:200, Life Technologies) for 30 min at RT ...
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bioRxiv - Biophysics 2020Quote: HEK293 cells stably expressing TfR-GFP were plated on fibronectin coated MatTek dishes and the next day incubated with 1 μM Alexa 647 labelled AP3 aptamer in blocking buffer (2 mM salmon sperm DNA, ThermoFisher #15632011), 2 mM yeast tRNA (ThermoFisher #AM7119 ...
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bioRxiv - Cancer Biology 2020Quote: ... Proteins were transferred by tank blot onto a PVDF membrane that was subsequently blocked in a solution containing 5% Blocking Buffer (Life Technologies) and 5% BSA (1:1 ...
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bioRxiv - Neuroscience 2022Quote: ... The treated cells were then incubated with blocking buffer at room temperature for 1 hour and primary antibodies (anti-NeuN (NC1284461, Fisher Scientific)) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: MuSCs cultured on biomimetic hydrogel and treated with thrombospondin-1 or with the thrombospondin-1 blocking antibody (A6.1, Thermo Fisher Scientific) for 6 days were pulsed with IdU at a concentration of 10μm for 1 hour and then harvested and processed for CyTOF staining as described above.
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bioRxiv - Neuroscience 2019Quote: ... The following day sections were washed 5×5 in 0.1M PBS and incubated at room temperature for 1 hr in the following secondary antibodies diluted 1:500 in blocking solution: goat-anti-Rabbit Cy3 conjugated IgG (Invitrogen A10520), goat-anti-chicken conjugated Alexa 488 (Invitrogen or A11039) ...
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bioRxiv - Molecular Biology 2020Quote: ... The blot was blocked in Intercept PBS blocking buffer (Li-cor) and subsequently incubated with RFP monoclonal antibody (ThermoFisher, MA5-15257) and probed with fluorescent anti-mouse secondary antibody ...
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bioRxiv - Cell Biology 2021Quote: ... and permeabilized with cold methanol at −20°C for 2 min or 0.1% TX-100 in PBS for 5 min at room temperature followed by blocking with 0.12% glycine (Fisher Scientific, G46-1) in PBS for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... three times and then blocking buffer (5% normal goat serum [Cat #PCN5000, ThermoFisher], 0.2% Triton™ X-100 [Cat #9002-93-1, Fisher Scientific] ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were rinsed with PBS and incubated in blocking solution for one hour with the appropriate secondary antibody conjugated to an Alexa-Fluor (4ug/mL, Thermo Fisher) along with the nuclear stain DAPI (Sigma ...
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bioRxiv - Immunology 2021Quote: ... were coated with 50 μL of 2 μg/mL per well of SARS-CoV-2 FL-S protein overnight at 4 °C prior to washing in PBS/Tween (0.05% v/v) and blocking with Blocker Casein in PBS (Thermo Fisher Scientific) for 1 h at room temperature (RT) ...
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bioRxiv - Cell Biology 2020Quote: ... They were then incubated with the appropriate dilution of primary and secondary antibodies (or nanobody) in blocking buffer consisting of 3% (wt/vol) BSA and 0.2% Triton X-100 (vol/vol; Thermo Fisher Scientific) in PBS ...
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bioRxiv - Immunology 2021Quote: ... Plates washed with PBS-Tween (PBS-T) and non-specific binding was blocked with TBS Startblock blocking buffer (Thermo Fisher Scientific). Serum samples were serially diluted 3-fold starting with a 1:50 dilution and added to the coated plates ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 (Invitrogen, Carlsbad, CA; 1:500).
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were washed with 1x PBS and secondary antibodies diluted in blocking solution were added 4 hrs at RT: anti-Rabbit 555 (1:800; Invitrogen; A31572), anti-Chicken Cy2 (1:800 ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibody was diluted to 1:3000 in blocking buffer and added to the plates followed by TMB substrate (Thermofisher Scientific). Upon stopping the reaction with 1M H2SO4 ...
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bioRxiv - Neuroscience 2020Quote: ... Isolated capillary networks were placed onto a glass coverslip and fixed (4% formaldehyde in phosphate buffered saline (PBS)) and blocked (0.5% Triton X-100, 5% SEABlock Blocking Buffer (ThermoFisher Scientific, USA) in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by incubation in goat anti-mouse Texas Red secondary antibody (20 μg/mL in blocking solution, Molecular Probes, Merelbeke, Belgium) and then in goat antirabbit FITC secondary antibody (10 μg/ml in blocking solution ...
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bioRxiv - Biochemistry 2022Quote: ... 3 times and the following secondary antibodies diluted in blocking buffer were used: Goat anti-rabbit Alexa Fluor 488 (1:500; Thermo Fisher), Cy3-conjugated AffiniPure donkey anti-mouse (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... Retinas were then washed five times with 1:1 blocking buffer in PBS for 20 min and incubated with Alexa Fluor streptavidin-conjugated antibodies (1:100; Life Technologies) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... then incubated with blocking buffer (10% normal goat serum–50062Z, Thermo Fisher; 0.5% BSA–BP1600, Thermo Fisher; 0.2% Triton X-100-T8787, Thermo Fisher) at room temperature for 15 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Permeabilized embryos were incubated in blocking buffer (0.2% Triton X-100 in PBS + 2% BSA (BSA Fraction V 7.5%, Gibco, cat.# 15260-037) + 5% goat serum (Jackson Immunoresrearch/ Dianova ...
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bioRxiv - Genomics 2022Quote: Samples were then blocked for 1 hour at room temperature in Blocking Solution (1x PBST, 10% Molecular-grade BSA (ThermoFisher AM2616)) ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were stained overnight with the following primary antibodies diluted in blocking buffer: mouse anti-neurofilament-M (1:1500, RMO270, Invitrogen, RRID:AB_2315286); rabbit anti-6xHisTag (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was blocked with 5% fat-free skim milk in TBST (Tris buffer saline with 0.05% Tween 20) or SUPERBLOCK T20 (TBS) Blocking Buffer (Thermo Fisher Scientific) then incubated with appropriate primary antibody overnight at 4°C ...
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bioRxiv - Physiology 2022Quote: ... Triton-X in PBS for three minutes followed by blocking with blocking buffer (1% w/v bovine serum albumin with 0.2% w/v keyhole limpet hemocyanin in PBS; Thermo Fisher Scientific) for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed three times with blocking solution for a total of 20 minutes and then incubated with secondary antibody (1:500, Invitrogen, #A31572) for 2h ...
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bioRxiv - Microbiology 2022Quote: ... The primary anti-body was washed away in PBS containing 1% DMSO (wash buffer) and larvae were incubated in blocking solution containing Goat anti-Rabbit546 (ThermoFisher #A11010) at 1:1000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... Coverslips were washed twice in blocking buffer and twice in TBS before mounting with ProLong™ Glass Antifade Mountant (Invitrogen P36982). Samples were visualized with a Zeiss LSM 710 META inverted laser scanning confocal microscope in part through the use of the Vanderbilt Cell Imaging Shared Resource ...
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bioRxiv - Neuroscience 2022Quote: ... tissue was washed in blocking solution and treated with secondary antibody (anti-guinea pig 488 1:50 and anti-chicken 594 1:300, Invitrogen, WA316328) for 2-3 days ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were then washed 3 times in cold PBS and blocked in FISH blocking buffer (2x SSC, 40ng/μL yeast tRNA (Life Technologies), 40ng/μL salmon sperm DNA (Life Technologies) ...
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bioRxiv - Biochemistry 2024Quote: ... RNA oligos were diluted to 100 nM in ELISA blocking buffer (PBS, 0.05% Tween-20, 0.2 mg/ml bovine serum albumin (BSA, BP9706100; Fisher Scientific; Hampton, NH)) ...
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bioRxiv - Cell Biology 2024Quote: ... they were rinsed three times in PBS and blocked for 30 minutes in blocking solution (PBS + 5% by volume normal goat serum, ThermoFisher PCN5000). Staining was for 1 hour with 1:500 anti-OSBP (Atlas Antibodies HPA039227 ...
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bioRxiv - Neuroscience 2024Quote: ... before being incubated with blocking solution (1× PBS with 5% Normal Goat Serum [50-062Z; ThermoFisher Scientific], 0.1% Triton X-100) for 15 min and with primary antibodies solutions for 2 to 3 hours at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated for 72-hours in the same blocking solution with anti-FR (1:500, Invitrogen #A6397, made in rabbit) and either anti-TH (1:5K ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were blocked with PLA blocking buffer and incubated with anti-NOD2 antibody (#MA1-16611, ThermoFisher, 1:500: RRID: AB_568643), anti-α-syn aggregate antibody (#ab209538 ...