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Citations for Thermo Fisher :
3601 - 3650 of 10000+ citations for Aldosterone Chemiluminescent ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... the concentration of Interferon-γ (IFNγ) in the media was measured by ELISA (Invitrogen, 88-7314). The T cells from 24-hour co-cultures were harvested ...
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bioRxiv - Immunology 2021Quote: ... The colorimetric reaction was terminated by the addition of 100 μL of ELISA Stop Solution (Invitrogen). The absorbance at 450 nm and 650 nm was read by spectrophotometry using a SpectraMax iD3 microplate reader (Molecular Devices).
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bioRxiv - Immunology 2019Quote: ... the concentration of IFN-γ in the cell culture supernatant was determined by ELISA (Invitrogen, #EHIFNG2). An IFN-γ standard was included in the ELISA and linear curve fitting using the GraphPad Prism 7.0 a software was used to derive the IFN-γ concentration in the samples from the absorbance at 450 nm (A450 ...
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bioRxiv - Cancer Biology 2020Quote: ... O.D values were determined at a wavelength of 590 nm using an ELISA reader (Thermo Scientific) The percentage survival was determined as the ratio of treated cells versus vehicle control after background subtraction ...
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bioRxiv - Microbiology 2021Quote: ... The assay reaction was developed with the 1-step Ultra TMB-ELISA substrate (Thermo Fisher Scientific). The reaction was left to develop for 5 min and then stopped with 2M H2SO4 ...
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bioRxiv - Biochemistry 2021Quote: ... Background binding level in ELISA assays was measured using Human IgG Isotype Control (ThermoFisher #02-7102).
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bioRxiv - Bioengineering 2021Quote: ... The signal was developed with 1-Step™ Ultra TMB-ELISA substrate solution (Thermo Fisher Scientific) and the reaction was stopped with 1% HCl ...
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bioRxiv - Neuroscience 2020Quote: ... Pierce HRP ECL substrate was used to detect luminescence in ELISA (Thermo Fisher Scientific, USA, #32209). Tris-buffered saline with Tween-20 (TBST ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were harvested and assayed for IL-8 by ELISA (Thermo Fisher Scientific #88-8086-77). Alternatively ...
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bioRxiv - Immunology 2022Quote: ... Colorimetric detection was accomplished using 1-Step™ Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific: 34029) and color development was quenched with 1.0 N HCl ...
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bioRxiv - Immunology 2022Quote: ... 25 μL/well of 1-step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, cat. # PI34029) was added to the plates and incubated for 5 to 10 min at RT ...
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bioRxiv - Immunology 2022Quote: ... The color reaction was developed using the 1-StepTM Ultra TMB-ELISA substrate solution (Thermo Scientific) and plates were read at 450nm using the Labtech LT 4500 microplate reader ...
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bioRxiv - Microbiology 2022Quote: ... followed by subsequent addition of Turbo 3,3′,5,5′ tetramethylbenzidine (TMB)-ELISA substrate solution (Thermo Fisher Scientific) and 2M sulfuric acid stop solution ...
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bioRxiv - Microbiology 2022Quote: ... Supernatants were used to perform ELISA to quantify the levels of IL-1β (Thermo Fisher Scientific), IL-6 (Biolegend) ...
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bioRxiv - Biochemistry 2024Quote: ... Signal was developed by treating cells with 200 μl TMB-ELISA (Thermo Scientific, Cat no. 34028) until the light blue colour appeared ...
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bioRxiv - Immunology 2022Quote: ... TNF concentrations in the supernatants were measured by ELISA (Fisher Scientific, Waltham, MA, Cat# 88-7346).
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bioRxiv - Biochemistry 2023Quote: ... 100 μl horseradish peroxidase substrate (1-Step™ Ultra TMB-ELISA Substrate Solution) (Thermo Scientific™) was added into wells followed by 100 ul 1M H2SO4 ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were harvested for IL-1β measurement using a specific ELISA (Thermo Fisher, 88-7621-22). Apoptotic cells were generated by retroviral transduction or MTX treatment during 20-24 h when cell death approaches its maximum ...
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bioRxiv - Pathology 2024Quote: ... The total amount of TGFβ1 was determined on technical duplicates by ELISA (88-8350-22, Invitrogen), after heat denaturation followed by acidic treatment and neutralization according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Absorbance was measured at a wavelength of 450 nm using an ELISA microplate reader (Thermo Fisher). Assays were repeated at least three times.
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bioRxiv - Immunology 2023Quote: ... supernatant was collected and IFN-ɑ measured by ELISA according to manufacturer’s instructions (Invitrogen; catalog # BMS6027)
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bioRxiv - Neuroscience 2024Quote: ... Levels of cerebral Aβ40/42 were determined by ELISA according to the manufacturer’s instructions (Invitrogen, CA). Furthermore ...
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bioRxiv - Bioengineering 2024Quote: ... Cystatin A levels were determined by enzyme-linked immunosorbent assay (ELISA) according to manufacturer’s instructions (Invitrogen) and calculated using standard curve interpolation ...
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bioRxiv - Biochemistry 2024Quote: ... was measured by ELISA of secreted mIFN-γ in cell supernatants (Invitrogen REF 88-8314-22).
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bioRxiv - Cell Biology 2019Quote: ... Samples were analyzed by SDS PAGE and Western blot using HRP-labeled secondary antibodies and SuperSignal West Pico chemiluminescent substrate (Thermo Fisher Scientific). Films with representative exposure times were scanned ...
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bioRxiv - Cell Biology 2020Quote: ... then incubated with appropriate primary antibodies and HRP or near-IR-conjugated secondary antibodies and visualized using chemiluminescence (SuperSignal West Femto Chemiluminescent Substrate; Thermo Fisher Scientific), or a Li-Cor Odyssey fluorescence scanner ...
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bioRxiv - Neuroscience 2019Quote: ... Target protein bands detection was done in Gel Documentation system (Syngene: chemiXX9) using Super signal West Pico Chemiluminescent substrate (Thermo Scientific, #34077) and densitometric quantitation assessed using Image Studio Lite v5.2 (LI-COR Biosciences).
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bioRxiv - Cancer Biology 2019Quote: ... The membranes were then washed with TBS-T buffer followed by exposure to the appropriate horseradish peroxidase-conjugated secondary antibody for 1h and visualized on either Kodak X-ray film (GeneMate, F-9023-8×10) or BioRad ChemiDoc Imaging System using either SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, 34080) or ECL Prime Western Blotting Detection Reagent (Amersham ...
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bioRxiv - Microbiology 2021Quote: ... samples were UV cross-linked and the biotin signal detected with HRP–conjugated streptavidin using the Chemiluminescent Nucleic Acid Detection Module (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... and revealed using HRP-conjugated antibodies (1: 5000 promega W401B and 1:10000 promega W4021) and the Super Signal Pico Chemiluminescent Substrate (Thermo Scientific, 34579). Signals were revealed on a Las3000 photoimager (Fuji ...
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bioRxiv - Cell Biology 2021Quote: ... was applied for 1 h at room temperature and the signal visualized using SuperSignal enhanced chemiluminescent (ECL) reagent (Thermo Fisher Scientific, #34080).
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bioRxiv - Cell Biology 2021Quote: ... and visualized using SuperSignal West Pico PLUS Chemiluminescent Substrate or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Biochemistry 2021Quote: ... The blot was washed three times (15 mL PBS-T, 10 min, rt) and WGA was visualized using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific).
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bioRxiv - Biochemistry 2022Quote: ... Immunoblots were visualized by film exposure using horseradish peroxidase-conjugated secondary antibodies and SuperSignal West Femto (DNMT3A) or SuperSignal West Pico PLUS (other proteins) chemiluminescent substrates (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... Protein bands were visualized using a LI-COR Biosciences Odyssey FC imaging system after the addition of SuperSignal West Pico chemiluminescent substrate (Thermo Fisher Scientific). Proteins were quantified with Image Studio Version 5.2.4 (LI-COR Biosciences) ...
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bioRxiv - Molecular Biology 2022Quote: ... the membrane was washed two times with TBS-T and one time with TBS (TBS-T without Tween-20) before SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific 34077) was used for imaging ...
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bioRxiv - Plant Biology 2022Quote: ... Biotinylated proteins or Myc-tagged miniTurbo were visualized on the membranes using a luminol-based chemiluminescent substrate that is oxidized by HRP in the presence of peroxide (Thermo Fisher; 34577). The membranes were stained with Coomassie staining solution (60 mg l-1 Coomassie brilliant blue ...
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bioRxiv - Microbiology 2022Quote: ... secondary antibodies (Kirkegaard & Perry Laboratories, Gaithersburg, MD) were used and visualized by SuperSignal West Dura chemiluminescent substrate or ECL (Thermo Fisher Scientific). All Western blots were performed with at least three biological and technical replicates and significance determined by t-test analysis.
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bioRxiv - Biophysics 2022Quote: ... Membranes were then washed three times before being subjected to SuperSignal West Pico PLUS Chemiluminescent Substrate or Dura Extended Duration Substrate (Thermo Fisher Scientific) peroxide solution treatment for visualization of antibody-bound proteins using a Bio-Rad ChemiDoc Touch Imaging System.
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bioRxiv - Immunology 2020Quote: ... the membrane was washed three times for 10 min in TBST and then treated with enhanced chemiluminescent substrate (Pierce™ ECL Western Blotting Substrate, ThermoFisher Scientific). Both gels and western blots were visualized using a ChemiDoc MP Imaging system (Biorad ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes were then washed three times with 1x TBST for ten minutes per wash and signals were measured using an ultra-sensitive enhanced chemiluminescent (Thermofisher Cat#34096) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... The membrane stained with Clean-Blot™ IP detection reagent was treated with SuperSignal™ West Pico PLUS Chemiluminescent Substrate (ThermoFisher 34577) for at least 5 min at room temperature before imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... signals were detected using ImageQuant LAS400 (General Electric) and images were captured using a SuperSignal™ West Pico Plus Chemiluminescent Substrate (Thermo Scientific) or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific).
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bioRxiv - Molecular Biology 2019Quote: ... The signal was detected by ECL using the SuperSignal West Pico Chemiluminescent Substrate or with SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher scientific) and detected with a BioRad ChemiDoc™ Xrs+ ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were further incubated with chemiluminescence substrate (SuperSignal West Femto Maximum Sensitivity Substrate, or SuperSignal™ West Pico PLUS Chemiluminescent Substrate) for 1 minute (Thermo scientific). Protein bands were visualized with the Fusion FX UILBER LOURMAT (Vilber) ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection of proteins was performed using secondary antibodies conjugated to horseradish peroxidase-HRP and the SuperSignal West Pico Plus chemiluminescent substrate (Thermo Scientific-Pierce).
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bioRxiv - Neuroscience 2021Quote: ... The membranes were washed five times with TBST and immunoreactivity was detected using SuperSignal West Pico PLUS Chemiluminescent substrate (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2020Quote: ... The membrane was washed for two minutes five times and incubated with chemiluminescent subtrate (SuperSignal™ WEST pico PLUS, Thermo Fisher Scientific) and imaged using a G:Box (Syngene) ...
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bioRxiv - Immunology 2020Quote: ... Detection of proteins was performed using secondary antibodies conjugated to horseradish peroxidase-HRP and the super signal west pico chemiluminescent substrate (Thermo Scientific-Pierce).
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bioRxiv - Cell Biology 2020Quote: ... were used as secondary antibodies and Western blots were developed using the SuperSignal™ West Pico Plus Chemiluminescent Substrate (Thermo Fisher Scientific) and an Image Quant LAS 400 (GE Healthcare ...