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Citations for Thermo Fisher :
3601 - 3650 of 6549 citations for 6 Chloro N methoxy N methyl nicotinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... Reactions were performed in a QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific), using SYBR Green chemistry (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... Quantification was performed in triplicate using the QuantStudio 6 Pro qPCR machine (ThermoFisher Scientific). Rpl19 was used as the endogenous control for relative quantification.
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bioRxiv - Immunology 2023Quote: Amplifications were performed with a QuantStudio 6 Pro Real-Time PCR System (Applied Biosystems). The assay lower LOD was 50 copies per reaction.
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bioRxiv - Cancer Biology 2024Quote: ... 1.25*105 cells/well were seeded in 6-well plates (Thermo Scientific cat# 140675) and for mitochondrial enrichment ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from duplicate wells of 6-well plates using TRIzol (Invitrogen) according to the manufacturer’s instructions and quantified using a BioAnalyzer (Agilent) ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were counterstained in 300 nM DAPI (4’,6-diamidino-2-phenylindole; D1306, ThermoFisher), and/or 20 μg ml-1 AlexaFluor 488-labeled peanut (Arachis hypogaea ...
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bioRxiv - Bioengineering 2024Quote: ... HEK293T cells were grown in a 6-well culture plate (ThermoFisher, cat. no. 140675) in DMEM with 10 % FBS (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were run on a QuantStudio 6 Flex 384-well qPCR apparatus (Applied Biosystems). Each target gene was normalized to HPRT ...
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bioRxiv - Cancer Biology 2024Quote: ... MSCs were used up to passage 6 and harvested by incubating with TrypLE (Gibco) to gently detach the cells from the culture surface.
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bioRxiv - Cancer Biology 2024Quote: ... sgRNA-infected cells cultured in 6-well plates were washed by DPBS (Gibco, #C14190500BT), followed by detachment using accutase (BioLegend ...
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bioRxiv - Microbiology 2024Quote: ... as well as DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) (1:1000; Invitrogen, #D1306) were diluted in DPBS with 1% FBS and incubated for 2 h at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific, 62248).
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bioRxiv - Molecular Biology 2024Quote: ... RNA was suspended in urea Dye and resolved on a 6% TBU gel (Invitrogen) at 200 V for 5 min ...
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bioRxiv - Immunology 2024Quote: ... Real-time PCR was performed in a QuantStudio 6 Flex PCR System (Applied Biosystems) using the Fast SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Neuroscience 2024Quote: ... on a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific, 4485689). A table with qPCR primer design is included (Supplementary Table 7).
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bioRxiv - Neuroscience 2024Quote: HeLa cells were plated in DMEM (Cellgro, #10-013CV) with 6% FBS (Gibco, #26140) at 10,000 cells per well in 96-well tissue culture-treated plates ...
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bioRxiv - Microbiology 2024Quote: ... the proteins were first treated with 6× Laemmli SDS sample reducing dye (Thermo scientific) and boiled for 5 min at 100 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... The slides were stained with 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) and mounted ...
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bioRxiv - Cell Biology 2024Quote: ... IMR90 PD 34 to PD 40 were seeded on 6 well plates (ThermoFisher, 140675) as the beginning points for the assays ...
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bioRxiv - Cancer Biology 2024Quote: ... All qPCR was run on QuantStudio 6 flex real-time PCR system (Applied Biosystems). Relative abundance was calculated with delta-delta-Ct method ...
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bioRxiv - Cell Biology 2024Quote: ... C2C12 cells on 6-well plates were transiently transfected with RNAiMAX (Invitrogen, 13778-150) according to the manufacturer’s directions in two stages to prevent over-confluency at the time of cellular treatment and lysis ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR was conducted on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using 12 ng cDNA per reaction ...
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bioRxiv - Cancer Biology 2021Quote: ... 6 hours prior to collection organoids were incubated with 1 μM EdU (Thermo fisher, #C10636).
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bioRxiv - Cell Biology 2020Quote: ... Cells in one well of 6 well plates were collected in ice-cold PBS (Gibco) and lysed in 200 µL lysis buffer (50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Cell Biology 2020Quote: ... RT-PCR was performed on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) with LightCycler 480 SYBR Green I Master (Roche) ...
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bioRxiv - Cell Biology 2020Quote: Live HUVECs and HEK293 cells were stained with Laurdan dye (6-dodecanoyl-2-dimethylaminonaphthalene) (ThermoFisher) at 10 μM (HUVECs ...
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bioRxiv - Immunology 2021Quote: ... vRNA was quantified using the QuantStudio 6 Real-Time PCR System (Applied Biosystems, Waltham, MA). Five microliters vRNA was added in duplicate to a 0.1 mL 96-well MicroAmp fast optical reaction plate (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were seeded at equal densities into wells of 6-well plates (Nunc, Roskilde, Denmark). The cells were pre-treated with EVs for 24 h and then stimulated with LPS (LL24 ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was quantified using Qubit high sensitivity assays (Thermo Fisher Scientific)6-10 μg of DNA was digested with 120 Units Csp6I enzyme (Thermo Fisher Scientific) [3-5 Csp6I digests per sample] overnight at 37°C in thermomixer shaking at 900 rpm (30s on ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were run on a QuantStudioTM 6 Flex Real-Time PCR System (ThermoFisher; Cat #: 4485691). Fold change calculations were done based on the ΔΔCt method ...
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bioRxiv - Neuroscience 2020Quote: ... stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Grand Island, NY, USA) for 15 minutes ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.05 % xylene cyanol) and the products were separated on a 6 % urea-PAGE gels (Invitrogen) run at 180 V for 50 min ...
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bioRxiv - Neuroscience 2022Quote: ... We plated an amount of 6×105 primary mouse cortical neurons (ref. Gibco A15586, ThermoFisher) on each coated coverslip ...
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bioRxiv - Neuroscience 2022Quote: ... We plated an amount of 6×105 primary mouse cortical neurons (ref. Gibco A15586, ThermoFisher) on each coated coverslip ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed in the QuantStudio 6 Flex Real-Time System (Applied Biosystems, Waltham, MA). Target gene expression levels were determined by the comparative 2∧-(delta C(T) ...
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bioRxiv - Neuroscience 2021Quote: ... and 5% (by volume) fluorescent bead solution (6 μm FocalCheck Microspheres; part no. F14807; ThermoFisher), prepared using deionized water ...
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bioRxiv - Neuroscience 2021Quote: ... fixed larvae were stained with 2.5 ug/ml 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) diluted in PBS to label all cell nuclei ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative PCR (qPCR) was performed using the Quantstudio 6 Flex (Applied Biosystems, Life Technologies, USA). For the qPCR reaction 1 μl of 1:20 diluted cDNA in MilliQ was used with 1 μl primer mix (forward and reverse primers ...
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bioRxiv - Molecular Biology 2020Quote: ... The mixtures were run on a QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific) with combined annealing and elongation cycles (Step1 ...
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bioRxiv - Molecular Biology 2019Quote: ... 6’-Diamidino-2-phenylindole (DAPI; Cat#D1306; RRID: AB_2629482) were purchased from Invitrogen (Carlsbad, CA). Neurobasal® medium ...
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bioRxiv - Immunology 2022Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) and LysoTracker Red DND-99 were purchased from Invitrogen, Carlsbad ...
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bioRxiv - Genomics 2020Quote: ... transduced cells were seeded into 6-well format in 1.5 μg/mL puromycin (Life Technologies) containing medium ...
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bioRxiv - Genomics 2020Quote: ... plated in 6-well plates then stimulated with anti-CD3/anti-CD28 Dynabeads (Life Technologies) over a period of 24-hours ...
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bioRxiv - Physiology 2019Quote: Total RNA was isolated from 6 adult female medaka (WT) pituitaries using Trizol (Ambion, USA) and cDNA was prepared from 30 ng total RNA ...
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bioRxiv - Cancer Biology 2020Quote: ... qPCR assays were performed on either QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) or CFX384 Touch Real-Time PCR Detection System (BIO-RAD) ...
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bioRxiv - Microbiology 2019Quote: ... nuclei were stained using Prolong gold antifade containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen). All preparations were observed with a confocal microscope (ZEISS LSM 700 inverted ...
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bioRxiv - Immunology 2019Quote: ... pneumoniae for 6 hours in 1% FCS phenol free alpha MEM (base media, Life Technologies). Cells were washed three times in PBS and gently lifted from the plate using a cell scraper in 300μl of base media supplemented with 1mM EDTA ...
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bioRxiv - Cancer Biology 2019Quote: ... then resuspended in the FACS buffer with 6 µg/mL Hoechst 33258 (Thermo Fisher; H3569) and either 2-µm YG microspheres (Polysciences ...
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bioRxiv - Synthetic Biology 2019Quote: Samples were resolved by electrophoresis in 6% or 15% polyacrylamide TBE-Urea gels (Thermo Fisher) in TBE ...
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bioRxiv - Synthetic Biology 2019Quote: ... Im7-6 was purified from IVG reactions using magnetic His-tag Dynabeads (Thermo Fisher Scientific). The IVG reactions were diluted in 90 µl of Buffer 1 (50 mM NaH2PO4 and 300 mM NaCl ...