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Citations for Thermo Fisher :
3601 - 3650 of 10000+ citations for 6 Bromo 3 N ethylamino 1 2 4 triazolo 4 3 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Samples were migrated on precast Novex 4–20% or 4–12% polyacrylamide gels (Thermo Fisher Scientific), then transferred to Novex nitrocellulose membranes (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and then fixed overnight at 4°C in 4% paraformaldehyde (Alfa Aesar, Thermo Fisher Scientific, UK), followed by several washes in 0.1M Phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2022Quote: ... samples were run on Bolt 4–12% SDS-polyacrylamide and native-PAGE 4-16% gels (Invitrogen) respectively ...
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bioRxiv - Biochemistry 2024Quote: ... samples were run on Bolt 4–12% SDS-polyacrylamide and native PAGE 4-16% gels (Invitrogen) respectively ...
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bioRxiv - Cell Biology 2023Quote: ... diluted in fresh DMEM for 4 h followed by fixation with 4% PFA (Thermo Fisher Scientific). To induce expression of the hGRAD and TRIM21 systems ...
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bioRxiv - Physiology 2023Quote: ... 0.5 mg protein was precleared for 30 minutes at 4°C with 4% Agarose beads (ThermoFisher). To immunoprecipitate RYR1 ...
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bioRxiv - Systems Biology 2024Quote: ... A Dionex IonPac AS11-HC-4 µm column (250 × 0.4 mm, 4 µm; Thermo Fisher Scientific) was used at 35°C to separate metabolite ...
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bioRxiv - Developmental Biology 2021Quote: ... Then embryos were incubated in the blocking solution for at least 4 hours and incubated overnight at 4°C with secondary antibodies anti-chicken-AF488 (Invitrogen, A11039, used at 1/800), anti-rabbit-HRP (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... slices were fixed with 4% paraformaldehyde at 4°C overnight and incubated with Alexa 647 coupled-streptavidin (Invitrogen S21374, 1:500 in PBS-10% tween) for 6 h at room temperature ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Cell Biology 2023Quote: ... on homemade coverslip-bottomed dishes were washed and loaded with Fura 2 by incubation with Fura 2 AM (4 μM, 45 min, 37°C, ThermoFisher, Cat# F1221), washed and imaged at room temperature in HEPES-buffered saline solution (HBSS) ...
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bioRxiv - Microbiology 2023Quote: ... the cultures were co-transfected with 2 µg of pCHMP-NS3-Flag and 2 µg of pGag using 4 µL of 293Fectin Reagent (Thermo Fisher Scientific). Cells were pelleted and high-pressure frozen as described in [68] using an HPM100 system (Leica Microsystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 and 6 were directly cloned into pCR(tm)2.1-TOPO® using the TOPO® TA Cloning® Kit (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Zoology 2020Quote: ... Tissues were cut into small pieces with a scalpel and washed twice in ADPBS containing antibiotics prior to placing 4-6 tissue pieces into a 25 cm2 plug-seal tissue culture treated flask (BioLite, Thermo Scientific) containing 1.5 mL establishment medium to cover the tissue pieces ...
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bioRxiv - Cell Biology 2019Quote: ... 50 μg of enriched mitochondria were resolved approximately 6 cm into a pre-cast 4–12% Bis-Tris polyacrylamide gel (ThermoFisher Scientific). The lane was excised and cut in 8 approximately equal chunks and the proteins reduced ...
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bioRxiv - Biophysics 2021Quote: ... cells were transiently transfected with 4 μg mouse Piezo1-pIRES-EGFP in pcDNA3.1(+) or 4 μg YFP 40-48 hours before recording in 6-well plates using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... 4 µg of plasmid was transfected with 4 µL P3000 regent and 6 µL Lipofectamine 3000 as per scaled recommendations in the manufacturer’s protocol (Thermo Fisher Scientific). Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol red-free DMEM + 10% FBS + 2 mM L-glutamine (Thermo Fisher) ...
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bioRxiv - Bioengineering 2021Quote: ... 4 µg of plasmid was transfected with 4 µL P3000 regent and 6 µL Lipofectamine 3000 as per scaled recommendations in the manufacturer’s protocol (Thermo Fisher Scientific). Medium containing lipid complexes was removed 4 h post transfection and replaced with exosome-depleted phenol-free DMEM + 10% FBS + 2 mM L-glutamine ...
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bioRxiv - Plant Biology 2020Quote: ... Sections were cut at 4-6 μm and dried from a drop of dH2O onto Probe On Plus slides (ThermoFisher Scientific). Tissues sections were stained with 0.01% aqueous Toluidine Blue O ...
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bioRxiv - Cell Biology 2022Quote: ... the cerebral cortices of 4 to 6 P0 rats were dissociated and chopped in Ca++/Mg++-free Hank’s Balanced Salt Solution (HBSS; Invitrogen, Carlsbad, CA). The tissue pieces were dissociated in phosphate-buffered saline (PBS ...
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bioRxiv - Developmental Biology 2021Quote: ... transferred to 1.6 mL Eppendorf tubes and spun at 2000 rcf for 5 minutes at 4°C in an angle rotor (Thermo Scientific Fisher Heraeus Biofuge Primo R Centrifuge) ...
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bioRxiv - Cell Biology 2019Quote: ... 50 μg of enriched mitochondria were resolved approximately 6 cm into a pre-cast 4–12% Bis-Tris polyacrylamide gel (ThermoFisher Scientific). The lane was excised and cut in 8 approximately equal chunks and the proteins reduced ...
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bioRxiv - Microbiology 2021Quote: ... samples were unfrozen and aliquots of 6 ml per sample were fixed with Electron Microscopy Sciences 4% Paraformaldehyde (5025999, Fisher Scientific), stained with SYBR™ Gold Nucleic Acid Gel Stain (S11494 ...
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bioRxiv - Biochemistry 2020Quote: ... 500 mM 6-aminohexanoic acid) was added to lysates right before loading samples into a NativePAGE Bis-Tris gel 4-16% (Life Technologies). The proteins were separated according to Wittig et al.32 and transferred onto a PVDF membrane using a standard Tris-glycine transfer buffer with 0.05% SDS ...
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bioRxiv - Neuroscience 2024Quote: ... The lentiviral construct of interest was co-transfected with the packaging constructs pCMV-VSV-G and pCMV Gag/Pol at a ratio of 10 µg: 4 µg: 6 µg respectively using Lipofectamine 2000 (60 µL, Invitrogen 11668027). After 4 h ...
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bioRxiv - Immunology 2023Quote: ... a total of 6 – 9 consecutive 10-µm coronal sections were taken from of 4 – 6 equally distanced regions along the length of the spinal cord using a Shandon Finesse 325 microtome (Thermo Scientific). Sections were mounted on Superfrost Plus microscope slides (Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The isolated primary mouse hepatocytes were plated onto collagen-coated 6-well plates at a density of 500,000 cells/well in 4 ml of DMEM (Thermo Fisher Scientific) plus 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2023Quote: ... Grids were blotted for 6 s with a blotting force of 6 at 4 °C and 100% humidity and plunged into liquid ethane using a FEI Vitrobot Mark IV (Thermo Fisher). The data were collected with a Glacios Cryo-TEM (Thermo Fisher ...
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bioRxiv - Genomics 2023Quote: ... for at least 6 hrs at 4 °C with rotation in 0.5 % BSA (Jackson Immunology, cat# 001-000-161) in PBS (Gibco, cat# 14190250), followed by blocking with 1% BSA in PBS for 1 hr at 4°C with rotation ...
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bioRxiv - Biophysics 2024Quote: ... grids were blotted once for 2.5 – 6 s (4 °C and 100 % humidity) using a FEI Vitrobot Mark IV (Thermo Fisher Scientific) and plunge-frozen in liquid ethane ...
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bioRxiv - Developmental Biology 2023Quote: ... we washed the mature COCs in synthetic oviductal fluid medium (SOF) containing N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES-TL, Thermofisher Scientific, Waltham, MA) and SOF for fertilization (SOF-FERT ...
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bioRxiv - Neuroscience 2022Quote: ... Hippocampal neurons 13-15 days-in-vitro (DIV) were transfected with plasmid (2 h, 2-3 µg per dish) with Lipofectamine 2000 (Invitrogen, #52887, minimum 24 h). For pH imaging experiments ...
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bioRxiv - Plant Biology 2020Quote: ... each slide was incubated overnight at 4°C with 100 μL of rabbit polyclonal anti-β-glucuronidase antibody (N-Terminal, 5420, Molecular Probes, Invitrogen) diluted 1:200 in fresh blocking buffer (3% BSA in 1X PBS) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated at 4°C overnight with the primary antibodies (Mouse anti-Dyrk1a: Abnova, Cat. N. H00001859-M01; Rabbit anti-Camk2a: Molecular probes, PA5-14315). After washing ...
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bioRxiv - Biochemistry 2023Quote: ... n=4) collected in microcentrifuge tubes were subjected to a 30-min incubation in 30 μM iodoacetamide (IAM) (Thermo Fisher Scientific, Waltham, MA) or N-ethylmaleimide (NEM ...
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bioRxiv - Biophysics 2020Quote: ... 4 mM glutamine GlutaMAX™ (Gibco) and 1x non-essential amino acids (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 mM L-glutamine (Life Technologies), 1 mM sodium pyruvate (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... 4 μL of Lipofectin (Life Technologies) and 1 μg of plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... Isopropanol (Thermo Fisher Scientific - A416-4), LDL (Alfa Aesar - J65039) ...
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bioRxiv - Neuroscience 2021Quote: ... and 4% FBS (10042682; Fisher Scientific) (Placzek et al. ...
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bioRxiv - Immunology 2021Quote: ... and 4 units of RNaseOUT (ThermoFisher). Following sorting ...
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bioRxiv - Bioengineering 2019Quote: ... 4 mM L-glutamine (Life Technologies), 10 mM HEPES (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... 100ng/ml IL-4 (Gibco, USA) for 48h ...
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bioRxiv - Neuroscience 2019Quote: ... and 4-12% NuPAGE (Thermo Scientific) Bis-Tris gels for ECC proteins ...
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bioRxiv - Cell Biology 2020Quote: ... 4 nM Calyculin A (PHZ1044; Invitrogen) or 1 mM 4-hydroxyacetophenone (278564 ...
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bioRxiv - Developmental Biology 2021Quote: ... on the Qubit 4 fluorometer (Invitrogen). RNA quality was assessed using the RNA 6000 Nano kit total RNA assay (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... 10 ng/ml IL-4 (Gibco), 10 ng/ml IL-10 (Gibco) ...
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bioRxiv - Bioengineering 2021Quote: ... in 4% horse serum (ThermoFisher, 16050122) and then incubated overnight with primary antibodies in 4% horse serum at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... 4 mM Glutamax (Thermo Fisher Scientific) and 1% v/v antibiotics/antimycotics (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... separated on 4%-12% NuPAGE (Invitrogen), and then electroblotted onto a polyvinylidenedifluoride membrane ...