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Citations for Thermo Fisher :
3551 - 3600 of 5129 citations for VAV2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Approximatively 200ng of each peptide sample were concentrated onto a C18 pepmap 100 (5mm x 300µm i.d.) precolumn (Thermo Scientific) and separated at 50°C onto a reversed phase Reprosil column (25cm x 75μm i.d. ...
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bioRxiv - Cancer Biology 2024Quote: ... Each peptide extracts were loaded on a 300 µm ID x 5 mm PepMap C18 precolumn (Thermo Scientific, USA) at a flow rate of 20 µL/min ...
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bioRxiv - Developmental Biology 2024Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Biochemistry 2024Quote: ... approximately 100 nanograms of peptide were analyzed by LC-MS/MS with an EASY-nLC 1200 (Thermo Fisher Scientific) and an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... tryptic peptide mixtures were analyzed by nano-scale high-performance liquid chromatography (Proxeon EASY-Nano system, Thermo Fisher Scientific) coupled with online nanoelectrospray ionization tandem MS (Q-Exactive HF-X mass spectrometer ...
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bioRxiv - Microbiology 2024Quote: ... peptides were subjected to electrospray ionization and loaded into either an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific) or a Velos Orbitrap Pro ion-trap mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... peptides were analyzed on a Q-Exactive HFX Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Rockford, IL, USA) coupled with a high-performance liquid chromatography system (EASY nLC 1200 ...
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bioRxiv - Microbiology 2024Quote: ... peptides were resuspended in 10.5 µL of 0.1% FA containing 40 fmol of DionexTM Cytochrome C digest (Life Technologies) (CytoC ...
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bioRxiv - Physiology 2024Quote: ... Peptides were loaded in buffer A (0.1 % formic acid) onto a 110 cm mPAC HPLC column (Thermo Fisher Scientific) and separated with a non-linear gradient of 1 – 50 % buffer B (0.1 % formic acid ...
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bioRxiv - Immunology 2024Quote: ... in presence of 0.1 μM [4Y]-MBP peptide in a final volume of 200 μl RPMI1640 + L-glutamine (GIBCO) containing 10% FCS and 1% penicillin/streptomycin (Life Technologies) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and then incubated in the dark for 1 h with secondary antibodies in 200 μl of blocking buffer (polyclonal goat anti-mouse Alexa Fluor® 488, 1:200 (A32723, ThermoFisher) and goat anti-rabbit Alexa Fluor® 647 ...
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bioRxiv - Immunology 2021Quote: ... Plates were blocked with blocking buffer made with 2% bovine serum albumin (BSA) Fraction V (Thermo Fisher Scientific, Waltham, MA, USA) and 1% gelatin from bovine skin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Coverslips were again washed twice in blocking buffer for 10min and mounted on slides with ProLong Diamond Antifade (Thermo Fisher #P36965) for imaging ...
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bioRxiv - Cell Biology 2020Quote: ... and treated with donkey Alexa Fluor 488-conjugated anti-rabbit IgG secondary antibody (A21206, Life Technologies; 1:1000 in blocking buffer) overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... They then had further blocking solution added to them containing Alexa Fluor 488/568 secondary antibodies (1:1000; Thermo Fisher Scientific) in addition to DAPI (1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... slices were washed in TBS-T and incubated for 1 h at RT with a mixture of the following secondary antibodies diluted 1:200 in blocking solution: anti-guinea pig Alexa Fluor 488 (Life Technologies) and anti-rabbit Alexa Fluor 568 (Life Technologies ...
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bioRxiv - Biophysics 2022Quote: ... in blocking buffer followed by 10 min incubation at room temperature with goat anti-rabbit IgG/horseradish peroxidase-conjugate (1:10,000 dilution of 1 mg/mL stock in blocking buffer) (A16110, Thermo Fisher Scientific). The blot was developed using enhanced chemiluminescence detection reagents (Pierce™ ECL Western Blotting Substrate ...
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bioRxiv - Bioengineering 2022Quote: ... The following secondary antibodies were used at 1:1000 dilution in blocking solution: Alexa Fluor 647 anti-Rabbit (Thermo Fisher, #A21244), Alexa Fluor 647 anti-Goat (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... A secondary antibody mixture was made using the same permeabilization/blocking solution with 1:1000 dilution of anti-mouse Alexa Flour 488 (Thermo Fisher) overnight at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... then incubated in blocking buffer composed of 1×PBS supplemented with 10% Gibco Fetal Bovine Serum (Thermo Fisher Scientific, Cat. A4766801) and 0.1% Triton X-100 ...
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bioRxiv - Neuroscience 2019Quote: To perform immunocytochemistry cells were fixated with 4% Paraformaldehyde and blocked with blocking buffer containing 5% Normal Goat Serum (Gibco®), 0,1% bovine serum albumin (SigmaAldrich ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were subsequently incubated for 1 hour at room temperature in blocking buffer containing the Alexa Fluor goat anti-mouse 488 or Alexa Fluor goat anti-rabbit 546 (Life Technologies) secondary antibodies (each at 1:1000 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... After a final wash in blocking solution the coverslips were mounted on glass slides by inverting them onto mounting solution (ProLong Gold antifade, Molecular Probes). For the characterization of the HEK293 Flag-BirA*-LUZP1 line ...
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bioRxiv - Biophysics 2020Quote: ... cells were incubated in blocking buffer with 4 ng/µl of multiple-labeled goat anti-rabbit IgG secondary antibodies (Invitrogen, 31212) with different DOLs for the α-tubulin ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated overnight (4°C) in the same blocking solution containing the primary rabbit anti-PV antibody (1:1000; Thermo Scientific) and mouse anti-SST antibody (1:250 ...
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bioRxiv - Plant Biology 2020Quote: ... Slides were washed three times for 5 min in 1X PBS solution and then incubated for 3 h at room temperature in 100 μL blocking buffer containing Alexa 488-conjugated goat anti-rabbit secondary antibody (Molecular Probes, Invitrogen), diluted 1:200 in fresh blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies were diluted in 0.5% BSA in PBS blocking solution: 1:400 ZO-1 (Cat#40-2200, Thermo Fisher Scientific); 1:1000 MUC5AC (Cat#MA5-12178 ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed in TBS-T buffer and then incubated for an hour at room temperature with the corresponding horseradish peroxidase-conjugated preadsorbed secondary antibody (1/2000, blocking solution corresponding, Molecular Probes). Membranes were washed in TBS-T buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then rinsed again in buffer solution and then immersed in secondary antibody solution (blocking solution and 1:500 donkey anti-mouse IgG conjugated with Alexa Fluor 488 (Thermo Fisher Scientific Cat# A-21202 ...
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bioRxiv - Neuroscience 2020Quote: ... was added together with labelled α-bungarotoxin (to visualise post-synaptic acetylcholine receptors) in blocking solution for 1.5 h at room temperature (RT) (α-btx, Life Technologies). Then ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then placed in blocking solution with secondary antibody [Alexa Fluor 790 Goat anti-Rabbit and/or Alexa Fluor 680 Goat anti-Mouse (Thermo Fisher)] at a dilution of 1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were washed in TBS-T buffer and then incubated for an hour at room temperature with the corresponding horseradish peroxidase-conjugated pre-adsorbed secondary antibody (1:5000, blocking solution corresponding, Molecular Probes). Membranes were washed in TBS-T buffer and immunoreactive bands were visualized with the SuperSignal West Pico Chemiluminescent Substrate (Pierce) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated in blocking solution for 2 h at room temperature with fluorescent donkey anti-goat alexa fluor 488 (1:500, Invitrogen, A11055) and goat anti-rabbit alexa fluor 647 (1:500 ...
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bioRxiv - Physiology 2021Quote: ... Slides were washed with blocking solution and incubated with appropriate secondary antisera (donkey or goat Alexa Fluor® 488, 546, 555, 633 or 647; Invitrogen) diluted 1:400 and Hoechst diluted 1:1500 for 1 hr ...
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bioRxiv - Neuroscience 2020Quote: ... This was followed by washing with PBS three times and incubating for 1h at RT in blocking buffer containing Alexa-Fluor-conjugated isotype-specific secondary antibodies (Thermo Fisher). Cells were washed three times with PBS and DAPI (0.4 μg/ml in PBS ...
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bioRxiv - Systems Biology 2020Quote: ... Then DNA oligo-conjugated primary antibodies listed below were incubated in the blocking solution with 100-fold diluted SUPERase In RNase Inhibitor (Invitrogen AM2694) at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed twice with PBS and blocked with the superblock blocking buffer in PBS (Thermo Scientific, catalog number 37515) for 1 h at 37°C ...
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bioRxiv - Systems Biology 2021Quote: ... with an estimated each concentration of 1-5 ng/μl were incubated in the blocking solution with 100-fold diluted SUPERase In RNase Inhibitor (Invitrogen AM2694) at room temperature for 18-24 hours ...
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bioRxiv - Neuroscience 2020Quote: ... then placed overnight at 4 0C in rabbit anti-GFP primary antibody (1:1000 in blocking solution, Invitrogen, La Jolla, CA). Following incubation ...
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bioRxiv - Microbiology 2021Quote: ... The plates were washed with PBS containing 0.05% Tween-20 (PBST) and blocked with Sea Block blocking buffer (Thermo Fisher Scientific) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 1 h (at RT with gentle shaking) in a blocking solution containing 10% normal goat serum (16210064, Life Technologies), 0.4% Triton X-100 and 0.1% glycine in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Slides were washed in blocking solution three times and incubated with goat anti-rat Alexa 488 secondary antibody (1:500 dilution, Molecular probes) at room temperature for 1 h ...
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bioRxiv - Systems Biology 2020Quote: ... The following primary antibodies were diluted in the blocking solution and applied overnight at 4°C: mouse anti-TAU clone HT7 (1:500, Thermo Scientific), chicken anti-MAP2 (1:10,000 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed twice with PBS and then incubated in blocking buffer containing the Alexa Fluor 488 secondary antibody (Life Technologies) (1:200 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were again washed with Blocking Buffer twice for 1 h and mounted on microscope slides using ProLong Glass (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... The cells were then incubated for 45 min with secondary antibody diluted in blocking buffer (1:500, Alexa Fluor 568, Molecular Probes) and washed twice with blocking buffer and once with PBS ...
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bioRxiv - Synthetic Biology 2022Quote: ... the gel was transferred to a 0.2 µm nitrocellulose membrane and blocked for 1 h at room temperature with SuperBlock™ blocking buffer (Thermo Scientific). Membranes were then washed four times in TBST buffer before incubation in a 1:5000 dilution of Monoclonal ANTI-FLAG® antibody (Monoclonal ANTI-FLAG® M2-Peroxidase (HRP ...
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bioRxiv - Biophysics 2022Quote: ... followed either by blocking and primary antibody staining or directly by 30 minute staining of nucleus (NucBlue Fixed Cell ReadyProbes, Life Technologies) and actin (ActinGreen 488 ReadyProbes ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were rinsed with PBS and incubated in a permeabilization/blocking solution of PBS containing 0.1% saponin and 5% goat serum (ThermoFisher Scientific 16210064) overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... incubated at room temperature for 2 hours in blocking buffer consisting of PBS supplemented with 10% bovine serum albumin (BSA; fraction V, Fisher Scientific), and then rewashed with PBS containing 0.05% Tween 20 ...