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Citations for Thermo Fisher :
3551 - 3600 of 10000+ citations for Progesterone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
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bioRxiv - Genetics 2021Quote: ... was purified from control and patient fibroblasts (seeded at 5 × 105 cells) using the PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific, K182001) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cDNA synthesis was performed with 0.5-5 μg of total RNA using the Maxima H minus Reverse Transcriptase kit (Thermo Scientific, Waltham, MA, USA) according to the manufacturer’s recommendations.
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragment was labeled with 5-(3-aminoallyl)dUTP by nick translation according to the manufacturer’s protocol (Ares DNA labeling kit, Molecular Probes, Eugene, OR, USA), and the incorporated dUTPs were labeled with amino-reactive Alexa Fluor 647 dye by using the same Ares DNA labeling kit ...
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bioRxiv - Microbiology 2020Quote: TaqMan RT-PCR was performed with the N2 primer/probe set and BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fischer Scientific) [31] using the QuantStudio 5 system (Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Molecular Biology 2020Quote: ... Fragments migrating above the 5 kb band of the marker were extracted using the Silica Bead DNA gel extraction kit (Thermo Fisher Scientific, K0513) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA of cells from cultures of 5-8 NT explants were extracted using the Ambion PureLink RNA Mini Kit (Invitrogen, cat. no. 12183018A) following the manufacturer’s guidelines ...
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bioRxiv - Genomics 2022Quote: ... Between 0.5-1 μg of RNA was further used for reverse transcription with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems cat.no. 4368814). Quantitative PCR was performed in triplicates using Fast SYBR™ Green Master Mix (Applied Biosystems cat.no ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 700 ng of total RNA was denatured at 65°C for 5 min and then reverse-transcribed using the Super-Script II kit (Invitrogen, Carlsbad, CA, USA) at 25°C for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... The DNA fragment was labeled with 5-(3-aminoallyl)dUTP by nick translation according to the manufacturer’s protocol (Ares DNA labeling kit, Molecular Probes, Eugene, OR, USA), and the incorporated dUTPs were labeled with amino-reactive Alexa Fluor 647 dye by using the same Ares DNA labeling kit.
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bioRxiv - Neuroscience 2024Quote: ... was generated with each of the 47 TaqMan probes (S1 Table 5) and Xeno primer (TaqMan Cells-to-Ct Control kit, Thermo Fisher Scientific, Sweden) and using the Taq-SSIII reaction mix (CellsDirectTM One-Step qRT-PCR Kit ...
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bioRxiv - Genetics 2023Quote: ... RNA was extracted in pools of 10 from 3-5 days old females randomly selected and unexposed to any insecticides using Arcturus PicoPure RNA isolation kit (Applied Biosystems, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: Cell cycle changes were additionally assessed using the 5-ethynyl-2’-deoxyuridine (EdU) assay using the Click-iT™ EdU Alexa Fluor™ 647 Flow Cytometry Assay Kit (ThermoFisher, C10418). HUH7.5 cells were incubated at 37 °C for 2 hours with 10 µM EdU using the Click-iT EdU Assay Kit (Thermofisher ...
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bioRxiv - Immunology 2023Quote: ... Cell suspensions were first stained for 5 minutes at room temperature with LIVE/DEAD® Fixable Red Dead Cell Stain Kit (ThermoFisher Cat# L23102). Surface proteins were then stained for 30 minutes at 4°C in FACS buffer (PBS containing 2% FBS and 0.1% NaN3 (MP Bio Cat# 2102891-CF)) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 μL ribodepleted RNA was mixed with 4 μL 5× TGIRT buffer (2.25 M NaCl, 25 mM MgCl2, 100 mM Tris-HCl, pH 7.5; Ambion RNase-free Buffer Kit AM9010), and 1 μL random hexamer primers (xGen) ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were subsequently washed in TBST and secondary antibodies conjugated to HRP were added in 5% milk and incubated for a minimum of 1 h at room temperature before developing with the ECL kit (Thermo Fisher Scientific, 34578).
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bioRxiv - Plant Biology 2020Quote: ... Four-day-old seedlings grown under constant white light (70 μmol s−1 m−2) were transferred to 96-well plates (136101 Nunc MicroWell White plate, ThermoFisher Scientific) with a 6 mm diameter dropper ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a 2 ml deep-well plate (1 ml of suspension per well, eight replicates per treatment) and a honeycomb plate (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... are thoroughly mixed and aliquoted column-wise according to the pattern of “Sample Plate 2” in a 96-well storage plate (AB0765, Thermo Fisher), using 100 µl of beads per well.
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bioRxiv - Molecular Biology 2020Quote: ... Nunc 96-Well Polypropylene DeepWell Storage Plates (cat. 278743) and 96-well Nunc MicroWell 96-Well Optical-Bottom Plates (cat. 265301) were purchased from Thermo Scientific. Molecular weight cut off filters were purchased from Millipore-Sigma ...
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bioRxiv - Developmental Biology 2019Quote: Cells were collected from either primed (E8 media on vitronectin-coated plates) or naïve (LIF-3i/MEF plates) conditions with Enzyme-Free Cell Dissociation Buffer (Gibco, 13151-014). Cells were washed in PBS and pelleted ...
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bioRxiv - Microbiology 2021Quote: ... Single cells were sorted on a BD FACSAria II into 96-well PCR plates (MicroAmp Endura Optical 96-Well Clear Reaction Plates, Life technologies) into lysis buffer as described (49).
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bioRxiv - Cancer Biology 2021Quote: ... 200nl of reconstituted siRNA from each well from the master plates was transferred to the same position of the corresponding black transparent bottom 384 well daughter plates (Thermo Fisher). Unused aliquoted plates were sealed with foil ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The plate was washed with PBS-T and the chemiluminescent signal was detected by a plate reader (Varioskan Flash, Thermo Scientific) after addition of ECL (1:1) ...
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bioRxiv - Cell Biology 2020Quote: ... PCR reactions were carried out on 96-well plates (MicroAmp®Fast Optical 96-well Reaction plate, Applied Biosystems, Life Technologies) and 2 μl cDNA (10-20 ng diluted in nuclease-free water ...
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bioRxiv - Cell Biology 2020Quote: ... PCR reactions were carried out on 96-well plates (MicroAmp®Fast Optical 96-well Reaction plate, Applied Biosystems, Life Technologies) and 2 μl cDNA (10-20 ng diluted in nuclease-free water ...
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bioRxiv - Immunology 2021Quote: ... in a 1:1 bead to T cell ratio and cultured in 1 ml (48-well plate) or 200μl (96-well plate) RPMI 1640 (Thermo Fisher, 21875) supplemented with 10% Fetal Bovine Serum ...
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bioRxiv - Neuroscience 2022Quote: ... The plate was washed with PBS-T and the chemiluminescent signal was detected by a plate reader (Varioskan Flash, Thermo Scientific) after the addition of ECL (1:1) ...
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bioRxiv - Cell Biology 2022Quote: ... 250.000 HEK293T cells were seeded per p35 plate (pre-coated Corning BioCoat Poly-D-Lysine 35 mm #354467 or uncoated Nunclon multidish 6 well plates, Thermo Scientific #140685 coated with 50 µg/ml Poly-D-Lysine solution (Gibco A38904-01 ...
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bioRxiv - Microbiology 2021Quote: ... The cells were mixed by inversion and 75µl of the suspension was dispensed to at least triplicate wells of 96-well blacked sided optical bottom plates culture plates (Thermo Scientific). At 40 hours post seeding ...
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bioRxiv - Cell Biology 2021Quote: ... were manually excised using 21G needles/scalpel blades and grown in low-binding 96 well plates (96 well Nunc Sphera Round Bottom Plates, ThermoFisher Scientific) in Retinal Differentiation Media (DMEM/F12 nutrient mix (3:1 ratio ...
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bioRxiv - Molecular Biology 2022Quote: ... 500ng circRNAs per well for 24-well plates (2 μg for 6-well plates) were transfected with lipofectamine MessengerMax (Invitrogen, LMRNA003) on the next day when the cell culture must have >90% viability and be 70% confluent ...
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bioRxiv - Systems Biology 2022Quote: ... 1 µl out of 1.1 µl of sample was picked up out of a 384 well plate (Thermo AB1384) placed on an auto sampler height adjuster for PCR plates (ThermoFisher 6820.4089) and sealed by an aluminum foil cover (ThermoFisher AB0626) ...
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bioRxiv - Cancer Biology 2022Quote: ... GSCs were cultured as spheres on non-tissue culture-treated 10cm plates or as adherent cultures on laminin on tissue culture-treated 10 cm plates (ThermoFisher Scientific). GSCs were grown in DMEM/F12 media ...
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bioRxiv - Synthetic Biology 2019Quote: Each plate containing the isolated clones was used to inoculate 200-µl aliquots placed on two replica 96-well plates for fluorescence measurement (Costar black plates with clear bottom; Thermo Fisher Scientific Inc. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 200 μl of each supernatant was transferred into a 96 well plate and the absorbance at 562 nm was measured using a Varioscan plate reader (Thermo scientific). In order to evaluate the degree of nanoparticle interference ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 μl of samples were transferred to a microwell plate (Nunc™ 3 84-Well Optical Bottom Plates # 242764, Thermo Scientific) and the time course of fluorescence-intensity (see Fig ...
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bioRxiv - Cancer Biology 2020Quote: ... by using a Fluorostar Omega plate fluorescence plate reader (540 nm excitation, 590 nm emission) after incubation with 10% Presto Blue (Life Technologies) for 1h at 37C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HK-2 was seeded in 6 well plates (1 × 106 cells/well) or 96 well plates (1 × 105 cells/well) and cultured in 90% RPMI1640 medium (Gibco, USA) supplemented with 100 U/mL penicillin ...
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bioRxiv - Cell Biology 2022Quote: ... pombe cells were pre-cultured to exponential phase in 180 μl of YES in 96-well culture plates at 32°C in the VarioSkan Flash plate reader (Thermo Scientific). 5 μl of the preculture were used to inoculate 175 μl of fresh YES in 96-well culture plates and incubated for ∼48 hours in the VarioSkan Flash plate reader at 32°C with background shaking (1080 spm ...
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bioRxiv - Systems Biology 2022Quote: ... filtered by positive pressure and the flowthrough was collected in a glass coated microtiter plate (WebSeal Plate+ 96-Well Glass-Coated, 300 - Fisher Scientific); 3 ...
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bioRxiv - Neuroscience 2023Quote: ... for ten minutes in a 96-well plate (protected from light) and the fluorescence was read at 520 nm using a Varioskan Flash plate reader (ThermoFisher, UK).
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bioRxiv - Neuroscience 2023Quote: ... neurons were dissociated and seeded (on coverslips inside a 6 well plate: 0.2E6 cells/well) onto plates containing NB plus [Neurobasal medium supplemented with B27 (Invitrogen GIBCO Life Technologies), GlutaMAX (GIBCO) ...
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bioRxiv - Plant Biology 2024Quote: ... 3 μl of the dilution series and cocktail mix were dispensed into duplicate wells on a 384-well ABI optical plate and the plate was loaded on the ABI 7900HT Fast Real-Time PCR System (Applied Biosystems). The FAM-Non Fluorescent Quencher detector and Rox passive reference were selected ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and then transfected with 100 ng/well (96-well plates) or 500 ng/well (24-well plates) indicated plasmids using Liopfectamine 3000 (Invitrogen, USA) for 24 h or as specified ...
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bioRxiv - Immunology 2024Quote: High-throughput screening of antibodies was done using Expi293 cells grown in 3 mL plates in 24 deep-well plates (Thermo Fisher). Cultures were grown for four days ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mM) and diluted in DMSO transferred to wells of PCR Plates (Micro Amp Fast 96-well Reaction Plates, Applied Biosystems) to ensure matching final DMSO concentrations.
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bioRxiv - Cancer Biology 2023Quote: ... siRNAs were transfected at 10ng/well in 384 well plates and 40ng/well in 96 well plates using Lipofectamine RNAiMAX (Invitrogen, #13778075). Imaging and drug incubation experiments were conducted in 384 well plates ...
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bioRxiv - Biochemistry 2022Quote: ... Bacterial growth was monitored by measuring OD600 and for growth experiments performed in airtight sealed microtiter plates (sealing tape for 96-well plates, Thermo Scientific), a Power Wave XS microplate reader (BioTek Agilent ...
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bioRxiv - Systems Biology 2023Quote: ... 150 μL aliquots of infected cells were deposited in a white 96-well plate (F96 MicroWell™ Plates, non-treated form Nunc) and the luminescent signal was measured with the help of the plate reader.