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Citations for Thermo Fisher :
3551 - 3600 of 10000+ citations for Mouse Anti Hepatitis B Surface Antigen Antibody 1837 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Membranes were then washed three times in TBS-T and incubated for 1 h at RT with goat anti-rabbit or goat anti-mouse HRP conjugated secondary antibodies (31460 and 31430, respectively, Thermo Fisher Scientific) diluted 1:10000 in TBS-T ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with fluorescent secondary antibodies (goat anti-rabbit Alexa Fluor 488, and/or goat anti-mouse Alexa Fluor 488; Invitrogen, 1:1,000), and finally washed with PBS three times for 5 min each ...
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bioRxiv - Cancer Biology 2023Quote: ... Fluorophore tagged secondary antibody (Alexa Fluor 568 anti-mouse IgG of Invitogen, A-11004 and Alexa Fluor 488 anti-rabbit IgG of Invitrogen, A-11001) were used in 1:200 dilution and incubated for 1 hrs at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then washed with PBS with 0.03% NaN3 for 15 mins RT × 4 and incubated in secondary antibodies at 1:1000 dilution (goat anti-mouse 488, Thermo Fisher Scientific# A-28175, goat anti-rabbit 647, Thermo Fisher Scientific# A-27040 ...
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bioRxiv - Cancer Biology 2023Quote: ... or 53BP1 clone 6B3E10 (Santa-Cruz) and visualized using Alexafluor-488-conjugated goat anti-rabbit and Alexfluor-546-conjugated goat anti-mouse antibodies (Invitrogen™, Life technologies) along with 4′,6-diamidino-2-phenylindole ...
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bioRxiv - Immunology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse secondary antibodies were used to assess protein signal (Thermo Scientific, Cell Signaling Technologies). Chemiluminescence was measured using an Odyssey Fc Imaging System (Licor ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were washed and incubated with either an AF488-conjugated goat anti-rabbit or an AF488-conjugated goat anti-mouse secondary antibody as appropriate (1:300, Thermo-Fisher Scientific, UK) in 1 % FCS ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were blocked in 6% BSA and immunolabeled with anti-α3 antibody (mouse Anti-ATP1A3 xVIF 9-G10, MA3-915, Invitrogen, Thermo Fisher scientific) for 2 hours 200 times in PBS1x containing 1%BSA or at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Cross-absorbed secondary antibodies Alexa Fluor™ 568nm- and 647nm-conjugated goat anti-rabbit and anti-mouse (Life Technologies #A11004 and #A21240) were used at 1/600 ...
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bioRxiv - Neuroscience 2024Quote: ... the same procedure was followed but using anti-β-Tubulin (1:1000, DSHB, USA) as the primary antibody and Goat anti-Mouse AF 546 (1:300, Invitrogen, Germany) as secondary antibody.
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bioRxiv - Molecular Biology 2024Quote: ... donkey anti-mouse Alexa Fluor 546 (1:500) or goat anti-rabbit Alexa Fluor 488 (1:500) secondary antibodies (Thermo Fisher Scientific). For staining with anti-centromere antibody ...
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bioRxiv - Microbiology 2024Quote: ... Sections were rinsed before incubation with a 1:1,000 dilution of an appropriate Alexa Fluor 546–conjugated (catalog A11018) or A488-conjugated (catalog A11070) goat anti-mouse or anti-rabbit antibody (Thermo Fisher Scientific). After a final wash with PBS ...
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bioRxiv - Cell Biology 2024Quote: ... rinsed 3 times in PBT and incubated for 4 h at room temperature with anti-mouse or anti-rat secondary antibody coupled to Dylight 488 (Thermo Fisher Scientific), or an secondary alpaca nanobody anti-mouse 488 SMS1AF488-1-100 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were then incubated in appropriate secondary antibodies (goat anti-rabbit-HRP, Invitrogen G21234, 1:10,000; goat anti-mouse IgG1, Invitrogen PA174421, 1:5,000) for 30 minutes at room temperature before washing and enhanced chemiluminescence with (SuperSignal™ West Femto Maximum Sensitivity Substrate ...
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bioRxiv - Cancer Biology 2022Quote: ... human antibody HLA-A,B,C (1:100, W6/32, #17-9983-42) (Thermo Fisher). To distinguish live/dead cells ...
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bioRxiv - Bioengineering 2021Quote: ... a glass surface with Tetraspeck beads (Thermo Scientific) was imaged with alternating 488 nm ...
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bioRxiv - Bioengineering 2022Quote: ... to image the cell surface and DAPI (Invitrogen) or Alexa Fluor™ 594-conjugated anti-human Prox1 (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... a glass surface with Tetraspeck beads (Thermo Scientific) was imaged with alternating 561 nm and 647 nm excitation to create a nanometer precise map to allow the correction of chromatic shift ...
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bioRxiv - Bioengineering 2023Quote: ... pre-coated surfaces in MCDB-131 medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2024Quote: ... and surface binding was detected using Alexa Fluor® 488 anti-IgG dye (Thermo Fisher Scientific; 1:500). Nucleus staining was done using DAOI or Hoechst ...
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bioRxiv - Immunology 2021Quote: ... Antigens were purified on C-tag column (Thermo Fisher) and quality-controlled by SDS-PAGE and for their level of endotoxin ...
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bioRxiv - Immunology 2020Quote: ... Antigens were retrieved using 2.5% trypsin (Thermo scientific, 15090046) in 0.1% HEPES buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... for antigen retrieval and 3% H2O2 (H325, Fisher Scientific) to inactivate endogenous peroxidase ...
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bioRxiv - Neuroscience 2023Quote: ... and antigen retrieved in citrate buffer pH 6.0 (ThermoFisher) in water bath at 80°C for 5 mins ...
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bioRxiv - Immunology 2020Quote: ... To be able to gate on NK cells the following surface antibodies were used: Live/dead aqua dead cell stain (Thermofisher), APC/Cy7 anti-human CD3 (HIT3a ...
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bioRxiv - Immunology 2020Quote: ... To be able to gate on NK cells the following surface antibodies were used: Live/dead aqua dead cell stain (Thermofisher), APC/Cy7 anti-human CD3 (HIT3a ...
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bioRxiv - Immunology 2021Quote: ... for 10 minutes prior to the addition or a surface antibody cocktail (Table S9) Cells were washed with FACs buffer and permeabilized with FoxP3 fix/perm (Invitrogen) overnight ...
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bioRxiv - Bioengineering 2022Quote: ... Single cell suspensions of pericytes were stained for 30 minutes at RT using fluorescence-labelled antibodies for pericyte surface markers (488-NG2 from Invitrogen, APC PDGFR-β and APC CD105 from Biolegend ...
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bioRxiv - Immunology 2022Quote: ... cells were stained with saturating concentrations of surface antibodies and a fixable viability dye diluted in 1:1 PBS (Invitrogen): Brilliant Violet Buffer (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... for 30 min at 37 °C and then surface stained at 4°C for 20 min with different combinations of surface antibodies in the presence of fixable live/dead stain (Invitrogen). Cells were then fixed and permeabilised (CytoFix/CytoPerm ...
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bioRxiv - Immunology 2023Quote: ... Cells were subsequently stained with surface antibodies and then either fixed using 1% paraformaldehyde or permeabilized using a FoxP3/Transcription factor fixation/permeabilization kit (ThermoFisher). Samples undergoing permeabilization were then stained with intracellular antibodies ...
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bioRxiv - Microbiology 2023Quote: ... permeabilized (for intracellular staining only) and then incubated or directly incubated (surface staining only) with FITC labeled MHC ABC antibody (Invitrogen) or PE labled CXCR4 (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivity to the antigen was visualized using Alexa Fluor 488- and Alexa Fluor 594-conjugated secondary antibodies (Molecular Probes, Eugene, OR). Nuclear staining was performed with Hoechst 33258 (Polysciences ...
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bioRxiv - Microbiology 2023Quote: ... The antibody-antigen complexes were incubated overnight at 4°C and eluted following Pierce Co-Immunoprecipitation (Co-IP) Kit (Thermo Fisher). Samples were then separated by gel electrophoresis followed by immunoblotting ...
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bioRxiv - Plant Biology 2024Quote: ... Chemiluminescence detection of antigen-antibody complexes was performed with SuperSignalTM West Pico or Femto Western substrate (Thermo Fisher Scientific, Darmstadt, Germany). As a loading control ...
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bioRxiv - Pathology 2023Quote: ... and then with the appropriate secondary antibody (Alex Fluor 488 anti-mouse IgG2b, Alex Fluor 555 anti-mouse IgG2a, or Alex Fluor 647 anti-rabbit IgG; 1:1,000, Invitrogen). Slides were observed using an LSM 710 Zeiss confocal laser microscope ...
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bioRxiv - Neuroscience 2023Quote: ... Sections stained with mouse primary antibodies were blocked in ReadyProbes “Mouse on Mouse” IgG blocking solution (Thermofisher) for 1 hour before the initial blocking step ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well multi-dish Nunc UpCell Surface cell culture plates or 10 cm Nunc UpCell Surface cell culture dishes (Thermo Fisher) and differentiated to human monocyte-derived macrophages (hMDMs ...
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bioRxiv - Physiology 2023Quote: Cell surface proteins from transfected HEK293 cells were biotinylated using the Pierce Cell Surface Protein Isolation Kit (ThermoFisher Scientific., cat# 89881). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... Alexa 555-conjugated mouse IgG antibody (secondary antibody; A28180, Thermo Fisher Scientific). Cell nuclei were stained with DAPI (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... Paraffin sections of all tumors and normal skin were deparaffinized and antigen retrieval was performed using heat induced epitope retrieval method (HIER) with 1X Antigen Retrieval solution (Invitrogen #00-4955-58) in a pressure cooker at 95 for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... or 568 in anti-mouse or anti-rabbit (Invitrogen). Coverslips were mounted onto glass slides using ProLong Gold Antifade Reagent (Cell Signaling Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... anti-rabbit and anti-mouse (1:1000, Molecular Probes) in blocking buffer containing 4ʹ,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-rabbit or anti-mouse magnetic beads (Thermo Fisher) were conjugated to antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... They were then rinsed in block for >30 min at room temperature before incubation in secondary antibody (1:250 Invitrogen #A21-42 AF488 anti-mouse IgM; 1:250 Life Technologies #A11029 AF488 anti-mouse IgG1; 1:250 Invitrogen #A21141 AF488 anti-mouse IgG2b) in blocking solution for > 1 hour at room temperature or overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... Alexa Fluor 488 anti-mouse IgG or Alexa Fluor 568 anti-mouse IgG (Thermo Fisher Scientific) at 1:500 for 2 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-mouse Alexa A647 (1:1000; goat anti-mouse IgG Alexa Fluor 647; Invitrogen #A21236) for 2h at RT ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated in 1:1000 anti-mouse AlexaFluor488 or anti-mouse AlexaFluor594 (Thermo Fisher Scientific) and 4’,6-diamidino-2-phenylindole counterstain (DAPI ...
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bioRxiv - Cancer Biology 2023Quote: ... and subsequently with fluorophore-labeled anti-mouse (goat anti-mouse Alexa Fluor 488; 1:2000; Invitrogen) and anti-rabbit secondary antibodies (goat anti-rabbit Alexa Fluor 594 ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-mouse-IgG2 Alexa 568 and goat anti-mouse-IgG1 Alexa 488 (1:500, Invitrogen).