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Citations for Thermo Fisher :
3551 - 3600 of 10000+ citations for Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Primary human CD4+ T cells were incubated with 20U/ml interleukin-2 (IL-2) alone or in combination with α-CD3/CD28 antibody-coated beads (100 μL/30 x 106 cells) (Gibco/Thermo Fisher) for 72 hours prior to infection with 100ng of p24 equivalent of pseudotyped pMorpheus-V5 ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 N and goblet cells were co-stained using rabbit anti-SARS-CoV-2 Nucleocapsid monoclonal antibody (HL453) (Invitrogen, cat# MA5-36272) (1:100 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed with 1 mL FCB to remove excess unbound antibodies and resuspended in FCB containing DAPI (Invitrogen D3571, dilution 1:10,000).
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bioRxiv - Microbiology 2021Quote: ... Unstained cell samples from each donor and compensation beads from the Invitrogen™ AbC™ Total Antibody Compensation Bead Kit (A10497; Thermo Fisher) were used to set voltages and create single stain controls ...
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Precision genetic cellular models identify therapies protective against endoplasmic reticulum stressbioRxiv - Neuroscience 2020Quote: ... cells were incubated for 30 min at RT with Alexa-488 labeled secondary goat anti-mouse antibody (ThermoFisher Scientific, cat. A32723, 1:1000), washed again twice with PBS and analyzed using flow cytometry on a FACSCelesta cytometer (BD Biosystems) ...
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bioRxiv - Pathology 2022Quote: ... anti dystrophin (Developmental Studies Hybridoma Bank MANDYS1 clone 3B7 cell culture supernatant) 25 % v/v antibody or anti Myosin-4 (Invitrogen, 14-6503-95) 1µg.mL-1 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed three-times with PBS and incubated with anti-mouse Alexa Fluor 594 labelled antibody (Invitrogen A-1105, diluted 1:500) for 30 min at RT ...
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bioRxiv - Developmental Biology 2020Quote: ... cells were stained with a mouse monoclonal antibody for cardiac isoform Ab-1 Troponin at 1:100 dilution (ThermoFisher Scientific #MS-295-P) or the isotype control antibody (ThermoFisher Scientific #14-4714-82 ...
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bioRxiv - Genomics 2021Quote: ... The cells were incubated with 1 mL diluted goat anti-rabbit secondary antibody with Alexa Fluor 488 (1:500 dilution) (Invitrogen, Cat# A-11008) in 5% BSA at 37 in 5% BSA at 37°C for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed three times with PBS and incubated with the secondary antibody (Goat anti-Rabbit Alexa Fluor 488, Thermo Fisher, 1:1000) and Alexa Fluor 568 Phalloidin (Thermo Fisher ...
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bioRxiv - Biophysics 2022Quote: A431 cells were resuspended at a concentration of 106 cells/mL and incubated for 15min in ice-cold starvation media containing either 40 μg/mL α-GFP antibody (A10262, Life technologies; Control) or 40 μg/mL DECMA-1 antibody (U3254 ...
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bioRxiv - Immunology 2022Quote: ... or three times (for anti-mCD180 antibody activated cells) and then suspended in prechilled 5% horse serum (Cytiva, SH3007403HI) solution in PBS with Ca2+/Mg2+ (Thermo Fisher Scientific, 14040133). After filtration (Falcon ...
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bioRxiv - Genetics 2022Quote: ... the cells were incubated with the appropriate secondary antibodies conjugated with Alexa Fluor 488 or Alexa Fluor 555 (Thermo Fisher Scientific; 1:500) for 30 min at room temperature ...
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bioRxiv - Immunology 2022Quote: Samples were FcR blocked then stained with antibodies and live/dead stain (LIVE/DEAD™ Fixable Near-IR Dead Cell Stain, Invitrogen, Cat#: L10119) for 30 minutes on ice shielded from light ...
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bioRxiv - Physiology 2022Quote: ... Cells were then incubated in Alexa Fluor 488 and Alexa Fluor 594-conjugated secondary antibodies against chicken (Molecular Probes Cat# A-11039, RRID:AB_142924) and rabbit (Thermo Fisher Scientific Cat# A-21207 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cells were washed 5x with blocking solution and a 1:500 dilution of secondary antibodies (Invitrogen Goat anti-rabbit IgG (H+L) Superclonal™/Invitrogen Alexa Fluor™ 594 goat anti-mouse IgG2a (γ2a ...
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bioRxiv - Neuroscience 2022Quote: The following secondary antibodies were used at a 1:1000 dilution for tissue and cell staining: donkey anti-rat Alexa Fluor 594 (Thermo Scientific A-21209), goat anti-rat Alexa Fluor 647 (Thermo Scientific A-21247) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were rinsed in PBS containing 10% FBS and centrifuged at 200xg for 5 minutes prior to labeling them with the anti-mouse CD31 monoclonal primary antibody (5ug/1×106 cells, Invitrogen, Carlsbad, CA, USA) in incubation buffer for 90 minutes at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed three times with 1X PBS and incubated with suitable fluorophore tagged secondary antibodies (1: 2000, Invitrogen, San Diego, CA, USA). Cells were washed with 1X PBS before mounting ...
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bioRxiv - Neuroscience 2022Quote: ... The following primary antibodies and dilutions were used: anti-GFAP (1:1000, Cell Signalling Technology, 3670) and anti-GFP (1:500, Molecular Probes, A-11122). The next day ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated for 45 minutes with secondary antibodies conjugated with Alexa Fluor 594 fluorescent dyes (Molecular Probes, 1:400 dilution in PBS). Next ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed and anti-rabbit Alexa Fluor 488 and anti-mouse Alexa Fluor 594 secondary antibodies were added (Thermo Fisher, MA, USA). After 1-2 hours ...
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bioRxiv - Microbiology 2022Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Microbiology 2022Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-N-WASP (Cell Signalling #4848S; 1:100) followed by Alexa Fluor 568 conjugated secondary antibodies (Invitrogen; 1:1000 in blocking buffer). Actin tails were labeled with Alexa Fluor 488 phalloidin (Invitrogen ...
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bioRxiv - Immunology 2023Quote: Another set of harvested lungs and spleen immune cells were stained with recommended dilutions of antibodies conjugated to fluorophores: CD3-FITC (Invitrogen, #11-0032-82) CD4-SB600 (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or antibodies was detected using an AF633 donkey goat anti-human IgG antibody (Iinvitrogen, A21091, 1/1000) and dead cells were stained using Live/Dead stain (Invitrogen, 15560607, 1/1000). Following 3 washes with PBS containing 0.5% BSA ...
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bioRxiv - Genomics 2023Quote: ... The human origin of PDX-derived cell lines was validated by flow cytometry using FITC anti-human EpCAM antibody clone VU-1D9 and (Thermo Fisher Scientific, #A15755) and PE/Cy7 anti-mouse H2Kd antibody clone SF1-1.1 (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 1 h with 488 (FLAG) and 594 (LCMV NP) Alexa Fluor antibodies (Life Technologies; 1:500 in blocking buffer), then washed four times with PBS.
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bioRxiv - Neuroscience 2023Quote: ... cells were washed three times in PBS and incubated with secondary antibody (Alexa-Fluor-647 goat anti-rat, Thermo Fisher, 1:200 dilution) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Immunology 2023Quote: ... cells were subsequently stained with a FITC-conjugated secondary goat anti-mouse IgG (H+L) polyclonal antibody (Invitrogen, cat# 62-6511; 1:200) or PE-conjugated F(ab’)2 goat anti-mouse IgG (H+l ...
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bioRxiv - Neuroscience 2023Quote: ... The neurons or the transfected cells were washed and reprobed with fluorescent conjugated secondary antibodies (anti-Rabbit AlexaFluor-568 1:1000 (Life Technologies, Carlsbad, USA) and anti-Mouse AlexaFluor-488 1:000 (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... samples were incubated in cell surface stains (a complete list of antibodies is included in table below) with either Fixable Viability Dye 780 (Invitrogen, 65086518, 1:4000) or Live/Dead Aqua (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... cells were then stained with 1:1000 secondary antibody Alexa647 for NS4B labeling (A-31573, donkey anti-rabbit, Thermo Fisher, Waltham, MA, USA), 1:4000 CellMask Orange (H32713 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were incubated with a secondary goat anti-rabbit IgG antibody (10–3) coupled to Alexa Fluor Plus 594 (Molecular Probes Life Tech.) for 60 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... APCs and patient-derived glioma cell lines were stained with HLA-A2 monoclonal antibody (Clone BB7.2, Thermo Fisher Scientific Cat. #17-9876-42). Approximately 1x106 cells per 100 μL FACS buffer (PBS supplemented with 1% BSA (Sigma Aldrich Cat ...
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bioRxiv - Microbiology 2023Quote: ... effector cells were stained with rabbit anti-SARS-CoV-2 S S1/S2 polyclonal antibody (Thermo Fisher Scientific, Cat# PA5-112048, 1:100). Normal rabbit IgG (Southern Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... cells were classified as Hypoxyprobe+ cells when they were labelled with the FITC pre-conjugated antibody and an Alexa Fluor™ 647 anti-mouse secondary (Invitrogen, cat. #A31571).
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bioRxiv - Immunology 2023Quote: ... and either stained with extracellular fluorescent antibodies at 4°C for 30 min and prior flow cytometric analysis SYTOX™ Blue Dead Cell Stain (1:1000, Thermo Fisher Scientific) was added ...
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CD56/NCAM mediates cell migration of human NK cells by promoting integrin-mediated adhesion turnoverbioRxiv - Cell Biology 2023Quote: ... The following antibodies and reagents were used for fixed cell staining at 1:100 dilutions: phalloidin Alexa Fluor 568 (Thermo Fisher Scientific A12380), CD56 Alexa Fluor 647 (clone HCD56 ...
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bioRxiv - Cancer Biology 2023Quote: ... Aliquots of transfected cells were retained for validation of HLA-A2 expression by staining with HLA-A2 monoclonal antibody (BB7.2, Thermo Scientific, 17-9876-42) and subsequent flow cytometry analysis.
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bioRxiv - Developmental Biology 2024Quote: ... the cells were incubated for 1 hour in the dark with anti-mouse Alexa Fluor 488 antibody (Thermo Fisher Scientific, catalog no. A28175) and anti-rabbit Alexa Fluor 594 antibody (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used to visualize cells after intracellular recording: anti-GFP polyclonal (1:1000, rabbit, ThermoFisher Scientific, MA, USA, LOT# 2477546) primaryantibody ...
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bioRxiv - Pathology 2024Quote: ... the cells were incubated for 45 min with Alexa Fluor 488-conjugated goat anti-mouse IgG (H+L) antibody (Invitrogen, Thermo Fisher Scientific, USA). Nuclear and kinetoplast DNA were stained with DAPI (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated with secondary antibody Alexa Fluor® 488-Goat anti-mouse IgG (Thermo Scientific. 1:500 in 1% BSA in PBS) for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were washed and stained with the following appropriate secondary antibodies: Alexa Fluor 594-goat anti-rabbit (Thermo Scientific, catalog no. A11012) and Alexa Fluor 488 goat anti-mouse (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 0.05% Triton X100 and incubated with secondary antibodies (Alexa 633 Goat anti-Rabbit antibody, Invitrogen A21071 ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibodies against the rabbit anti-GFP antibody was conjugated with Alexa Fluor 488 (Invitrogen) diluted at 1:500 in dilution buffer and incubated for 2 hours at room temperature or overnight at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... primary antibodies were detected with HRP-conjugated secondary antibodies and the TSA system (Molecular Probes). Embryos from the primary screen were live imaged on an Axio Observer 5 compound fluorescent microscope ...