Labshake search
Citations for Thermo Fisher :
3551 - 3600 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... Protein concentration of lysates was determined with the Pierce 660 Protein Assay Reagent (Thermo Scientific, Rockford, IL).
-
bioRxiv - Neuroscience 2020Quote: ... The tissue was homogenized and sonicated in T-Per lysis buffer (Thermo Scientific, Rockford, IL, United States). The protein concentration of the samples was determined using a Pierce BCA Protein Assay Kit (Thermo Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... Protein levels of IL-18 from intestinal biopsies were measured by ELISA (BMS618-3, ThermoFisher, Waltham, MA). Assays were performed according to the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2019Quote: ... Cell nuclei were counterstained with 0.5 μg/ml DAPI for 5 min (Thermo Fisher Scientific, Rockford, IL). The following primary antibodies were used ...
-
bioRxiv - Physiology 2021Quote: ... IL-6 (assay ID: HS00985639_m1) and TNF-α: (assay ID: HS00174128_m1) following manufacture’s protocol (Thermo Fisher Scientific). 18s (assay ID ...
-
bioRxiv - Cancer Biology 2020Quote: ... RNA was quantified using the Qubit™ RNA High Sensitivity Assay Kit (Thermo Fisher Scientific, Rockford, IL). Sample integrity was evaluated using the Agilent Bioanalyzer RNA Nano and Pico kits (Agilent Technologies ...
-
bioRxiv - Cell Biology 2020Quote: ... secondary antibody (diluted 1:10,000) and SuperSignal® West Femto Maximum Sensitivity Substrate (Thermo Scientific, Rockford, IL).
-
bioRxiv - Biophysics 2020Quote: ... or Zeba micro spin™ desalting colums 0.5 ml (7000 MWCO, Thermo Fisher Scientific, Rockford, IL, USA) were used ...
-
bioRxiv - Immunology 2022Quote: ... Protein was visualized using the SuperSignal West Pico ECL kit (Pierce – Thermo Fisher Scientific, Rockford, IL, USA).
-
bioRxiv - Molecular Biology 2020Quote: ... RNA was reverse transcribed with the SuperScript IV First-Strand Synthesis System (Thermo Fisher Scientific, Rockford, IL). cDNA was PCR amplified using the mEX11F and mEX13R primers and subjected to Sanger sequencing.
-
bioRxiv - Biochemistry 2021Quote: ... 0.1% SDS) supplemented with 1X Halt protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, Rockfold, IL, USA) and 1mM phenylmethylsulfonyl fluoride (PMSF ...
-
bioRxiv - Molecular Biology 2020Quote: Chromatographic separation was achieved on an Accucore C30 column (2.6µm, 3mm x 150mm, Thermo Scientific, Rockford, IL) with linear gradient elution consisting of mobile phases A (water/acetonitrile = 40:60 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was measured using a BCA reducing agent compatible assay kit (Thermo Scientific, Rockford, IL, USA). Proteins were precipitated by adding ice-cold acetone overnight ...
-
bioRxiv - Cancer Biology 2020Quote: ... Calibration was performed before each analysis using a positive calibration mix (Piercenet – Thermo Fisher, Rockford, IL, USA). Limits of detection (LOD ...
-
bioRxiv - Developmental Biology 2022Quote: ... The ATP concentration of the supernatant was calculated by the ATP Determination Kit (Thermo Scientific, Rockford, IL) relative to a standard ...
-
bioRxiv - Synthetic Biology 2019Quote: The optical density was measured at 600 nm using a spectrophotometer (GENESYS 30, Thermo Scientific, IL, USA). The dry cell mass was obtained by multiplication of the optical density of culture broth with a pre-determined conversion factor ...
-
bioRxiv - Microbiology 2019Quote: Amounts of IFNγ and IL-6 in BALF were estimated by ELISA Ready-SET-Go! Kit (Invitrogen) according to manufacturer’s protocol.
-
bioRxiv - Cancer Biology 2019Quote: ... cells were homogenized and resuspended in radioimmunoprecipitation assay (RIPA) lysis buffer (Thermo Fisher Scientific, Rockford, IL, USA) containing a cocktail of protease inhibitors (Roche ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Values were corrected for protein concentration as determined with a BCA assay kit (Thermo Scientific, Rockford, IL). All values were determined in triplicate ...
-
bioRxiv - Physiology 2019Quote: ... Gels were washed and proteins were stained with Imperial Protein Stain (Thermo Fisher Scientific, Rockford, IL, USA) for 2 hours ...
-
bioRxiv - Immunology 2019Quote: ... IL-2 mRNA amounts were determined with a SYBR Green PCR master mix from Life Technologies (4344463) relative to mRNA for β-2 microglobulin on a DNA Engine Opticon II System (Bio-Rad ...
-
bioRxiv - Physiology 2019Quote: ... The quantity of the RNA was determined on a Nanodrop ND-1000 (Thermo Scientific, Rockford, IL, USA). Reverse-transcriptase PCR was performed using the Multiscribe Reverse Transcriptase method (Applied Biosystems ...
-
bioRxiv - Cell Biology 2019Quote: ... and eluted with imidazole (gradient 10–200 mM) and dialyzed (prod. no. 68100, Thermo Scientific, Rockford, IL). In the case of Sec14 ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein concentration of cells was measured using a Micro BCA Protein Assay Kit (Thermo Scientific, Rockford, IL) and used to normalize cytokine levels with final concentrations adjusted to an equivalent of 25 μg of protein for comparison across experiments.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Total protein content in the homogenate was estimated using the BCA assay (Thermo Fisher Scientific, Rockford, IL), according to the manufacturer’s instructions ...
-
bioRxiv - Physiology 2019Quote: ... The blots were then developed using Super Signal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, Il, USA) and signals were captured by using high sensitive CCD camera-based imager (BioSpectrum Imaging System) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Equal amounts of conditioned media were analyzed by ELISA (IL-1 beta Human ELISA Kit, Thermofisher KHC0011), using manufacturer protocol ...
-
bioRxiv - Biochemistry 2021Quote: ... Chemiluminescent kit was a Supersignal West pico plus chemiluminescent substrate (34577) purchased from Thermo Scientific (Rockford, IL).
-
bioRxiv - Synthetic Biology 2021Quote: The optical density was measured at 600 nm using a spectrophotometer (GENESYS 30, Thermo Scientific, IL, USA). The dry cell mass was obtained by multiplication of the optical density of culture broth with a pre-determined conversion factor ...
-
bioRxiv - Pathology 2021Quote: ... hMADS adipocytes were differentiated in 2-well Lab-Tek Chamber Slides (Nalge Nunc International, Naperville, IL, USA), washed with PBS pH 7.4 and fixed with 10% paraformaldehyde in PBS for 10 min at RT ...
-
bioRxiv - Cancer Biology 2021Quote: ... blocked and permeabilized in a 10% BSA solution with Triton- X (Thermo Fisher Scientific, Rockford, IL, USA). Subsequently ...
-
bioRxiv - Bioengineering 2022Quote: Samples were denatured and reduced using 5× Lane Marker Reducing Sample Buffer (Thermo Scientific, Rockford, IL, US), boiled at 95°C for 10 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... Eight drops of the suspension (2 µl) were placed on Polysine slides (Thermo Scientific/Pierce, Rockford, IL), dried ...
-
bioRxiv - Microbiology 2022Quote: ... DNA concentration was measured with the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific, Rockford, IL, USA); DNA integrity was checked by gel electrophoresis.
-
bioRxiv - Physiology 2022Quote: ... and then stained for markers at 5 μg/ml for IL-17A-FITC (Thermofisher, 11-7177-81) and IFNg-PE (Thermofisher ...
-
bioRxiv - Bioengineering 2024Quote: ... T-cell cultures were supplemented with 200 U/mL IL-2 (Cat # 200-02, Peprotech (Thermo Fisher), Cranbury ...
-
bioRxiv - Neuroscience 2024Quote: ... cells were fixed and immunostained for Iba-1 and cleaved IL- 1β (1:100, PA5-105048, Invitrogen) or CD68 and cleaved caspase 1 (1:200 ...
-
bioRxiv - Immunology 2023Quote: Sandwich enzyme-linked immunosorbent assay detection (ELISA) kit for human IL-1β (Invitrogen, catalog # 88-7261-88) was used at the specified temperature and conditions according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2023Quote: ... and serum IL-6 and TNF-α levels were measured using a commercial ELISA kit (Thermo Fisher). Serum CD5L levels were measured using ELISA ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by permeabilization for 3 min with 0.15% Surfact-Amps X-100 (28314, Thermofisher Scientific, Rockford, IL) in PBS ...
-
bioRxiv - Biophysics 2023Quote: ... human IL-2 (Cat# PHC0026) and CellTrace™ CFSE (Cat# C34570) were purchased from ThermoFisher (Waltham, MA). Bovine serum albumin (BSA ...
-
bioRxiv - Microbiology 2023Quote: ... the concentration of peptides was measured using Pierce Quantitative fluorometric peptide assay (ThermoFisher Scientific, Rockford, IL, USA).The resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... following chemiluminescent detection (SuperSignal® West Pico PLUS and West Femto Chemiluminescent Substrate (Thermo Scientific, Rockford, IL).
-
bioRxiv - Cancer Biology 2023Quote: ... ANBL-6 cells were also supplemented with 5 ng/ml IL-6 (Thermo Fisher Scientific, Cat# 206IL010).
-
bioRxiv - Physiology 2023Quote: ... membrane was washed and developed using a SuperSignalTM west pico plus chemiluminescent substrate (Thermo Scientific, IL, USA). The blot was developed in iBright 1500 imaging system (Invitrogen ...
-
bioRxiv - Neuroscience 2023Quote: ... Some of the blots were then stripped (Restore PLUS Western Blot Stripping Buffer; Thermo Scientific, Rockford, IL) and reblotted if needed.
-
bioRxiv - Genomics 2023Quote: Quantitative measurement of water-soluble protein performed using Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL) [26] ...
-
bioRxiv - Immunology 2024Quote: ... The supernatant was collected on day 3 and analyzed by human IL-2 ELISA Kit (Thermo Fisher) according to the manufacturer’s protocol.
-
bioRxiv - Cancer Biology 2024Quote: ... qPCR quantification of miRNA expression was performed using TaqMan MicroRNA® Assays (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2024Quote: Murine TNF and IL-1β were quantified in cell supernatants using the ELISA™ Kit from Invitrogen and human IL-1β was similarly quantified with the Instant ELISA™ Kit from Invitrogen ...