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Citations for Thermo Fisher :
3501 - 3550 of 8181 citations for TSLPR Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... EVs were resuspended in D-MEM (0 % FCS) + fluorescent Bodipy Phosphocholine analogue at 2 μg/mL (D3793, Thermofisher Scientific) and incubated overnight at 4°C ...
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bioRxiv - Pathology 2021Quote: Cryosections were permeabilized and blocked for 1 h at RT in 0.1% PBTx and 10% fetal calf serum (FCS; Invitrogen), and incubated O/N at 4 °C with the primary antibodies in 0.1% PBTx plus 1% FCS ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% (v/v) heat-inactivated fetal calf serum (FCS) and 1% (v/v) Penicillin-Streptomycin (P/S, 10.000 U/ml, Gibco). Jurkat ...
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bioRxiv - Microbiology 2021Quote: ... or 20% (THP-1) (v/v) heat-inactivated fetal calf serum (FCS) and 1% (v/v) Penicillin-Streptomycin (10.000 U/ml, Gibco). THP-1 monocytes were treated with 100 nM 12-O-tetradecanoylphorbol-13-acetate (TPA ...
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bioRxiv - Cell Biology 2021Quote: Dulbecco’s modified Eagle’s medium (DMEM) and DMEM-F12 containing 10% heat-inactivated fetal calf serum (FCS) (Thermo Fisher Scientific) were used to culture human HEK293T ...
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bioRxiv - Immunology 2022Quote: ... Extracellular antibodies were diluted in FACS buffer (2% FBS and 5mM EDTA in PBS) with Fc Block (Life Technologies) and incubated for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... cell pellet was re-suspended in ice-cold proliferation growth medium (PGM) containing DMEM supplemented with 10% FCS (Invitrogen), 10% horse serum (Invitrogen) ...
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bioRxiv - Physiology 2022Quote: ... and grown for 4 days on untreated tissue culture plates in a humidified incubator with 5% CO2 at 37°C with culture medium (RPMI, 10% heat-inactivated FCS, 50 units/mL penicillin; 50μg/ml streptomycin -GIBCO) supplemented with 50 ng/ml rhM-CSF (Sino Biologicals) ...
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bioRxiv - Microbiology 2022Quote: ... with 7⍰.5% heat-inactivated fetal calf serum (FCS; Life Technologies with 1% penicillin/streptomycin (PS, 5000U.mL−1 and 5000μg.mL−1 respectively; Life Technologies) and supplemented with 1⍰% non-essential amino acids (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 μL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... and cells were stained with Fixable Viability Dye (eBioscience) and primary antibodies in the presence of Fc block in flow buffer (Dulbecco’s phosphate-buffered saline [Gibco] supplemented with 2% fetal calf serum (FCS) ...
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bioRxiv - Molecular Biology 2021Quote: ... Mouse embryonic fibroblasts (MEF) were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% heat-inactivated fetal calf serum (FCS, GIBCO), 10 U/ml penicillin/streptomycin ...
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bioRxiv - Molecular Biology 2021Quote: ... Germany) were cultivated in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FCS, Biochrom or GIBCO), 100 U/ml of penicillin and 0.1 mg/ml of streptomycin (PAN-Biotech) ...
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bioRxiv - Microbiology 2021Quote: ... J/4320/15) and the absorbance at 450 nm was read on a Multiskan FC Microplate Photometer (Thermo Scientific). Titres were determined by curve fitting data to a 4PL curve in GraphPad Prism 9 and accepting only data which produced an R2 value of >0.95 ...
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bioRxiv - Microbiology 2021Quote: ... 30-2003, ATCC) supplemented with 10% heat-inactivated fetal calf serum (FCS, Hyclone, USA) and 1% pen/strep (Invitrogen) at 37°C in a humidified 5% CO2 incubator (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... These cells were washed with PBS and then resuspended in PBS supplemented with 2% FCS or FBS and 5µM CM-H2DCFDA probe (Invitrogen) and incubated in the dark for 20min at 37°C under 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... with 7 .5% heat-inactivated fetal calf serum (FCS; Life Technologies with 1% penicillin/streptomycin (PS, 5000U.mL−1 and 5000µg.mL−1 respectively; Life Technologies) and supplemented with 1 % non-essential amino acids (Life Technologies ...
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bioRxiv - Immunology 2022Quote: ... They were cultured in RPMI 1640 medium supplemented with l-glutamine and 10% FCS (v/v) (Gibco, Massachusetts, USA). Vδ2+ γδ T cell expansion was stimulated using 5 μM zoledronic acid (Actavis ...
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bioRxiv - Cell Biology 2022Quote: ... The absorbance of each well was measured with a microplate reader (Multiskan FC, Thermo Fisher Scientific, Waltham, MA, USA) set to 450 nm ...
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bioRxiv - Immunology 2020Quote: ... pH 7.2) resuspended and incubated for 30 min in FACS buffer supplemented with Fc gamma receptor CD16/CD32 antibodies (ThermoFisher). Cells were then incubated with Alexa fluor 488-conjugated antibodies against F4/80 or rat IgG2a kappa isotype control ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissues were immersed in 2ml dissociation solution (2% FCS-PBS solution with approximate 145U/ml type 4 Gibco collagenase) and incubated at 37°C for 30 minutes ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... filtered through a 70 µm cell strainer, washed with FACS buffer (PBS supplemented with 2% FCS, 2 mM EDTA and 0.01% Sodium Azide (all Gibco)) and maintained at 4°C ...
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bioRxiv - Immunology 2021Quote: ... in R10 media (RPMI 1640 with 10% FCS, 1% penicillin/ streptomycin, and 2 mM L-glutamine, Thermo Fisher Scientific) at 37°C in 5% CO2 to differentiate quiescent MBCs into mASCs ...
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bioRxiv - Immunology 2021Quote: ... FACS-sorted cells were suspended at 0.5×106 cells/mL in RPMI 1640 medium supplemented with 10% HI-FCS and 1% PenStrep (10378016; Gibco). CD14+HLA-DRneg/low/CD14+HLA-DRhigh monocytes were cultured in 96 well plates (200μL/well ...
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bioRxiv - Immunology 2021Quote: ... The hACE-2-Fc content in the supernatant was quantified by ELISA using 96-Well MaxiSorp Immuno plates (ThermoFisher) coated overnight with 200 ng/well of RBD219-WT protein ...
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bioRxiv - Immunology 2022Quote: ... as per the manufacturer’s instructions.(Heideveld, Hampton-O’Neil et al. 2018) CD14+ cells were stored frozen in 50% foetal calf serum (FCS, Gibco), 40% PBS with 10% DMSO (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: HeLa cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) with Glutmax and 5% FCS (Gibco) and passaged every few days ...
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bioRxiv - Cell Biology 2022Quote: ... for 10 min at 37°C followed by trituration in DMEM supplemented with 10% FCS and penicillin/streptomycin (Gibco). Dissociated cells from one embryonic heart were plated on one glass coverslips (diameter 12 mm ...
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bioRxiv - Synthetic Biology 2022Quote: ... Idiotype antibodies and Fc-proteins were fluorophore-conjugated using DyLight 650 or DyLight 488 Microscale Antibody Labeling Kits (Invitrogen). ROR1 scFvs also contained a VSV-g tag that were detected by anti-VSV-g polyclonal antibody (FITC ...
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bioRxiv - Cancer Biology 2024Quote: ... BE(2)-C cells and SK-N-AS cells were grown in 75 cm2 cell culture flasks in Dulbecco’s Modified Eagle’s Medium (DMEM) + 10% fetal calf serum (FCS) (Life Technologies), and stable ATP13A3 KD and overexpression SH-SY5Y cells were cultured in DMEM with high glucose supplemented with sodium bicarbonate ...
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bioRxiv - Physiology 2024Quote: For MPS 4.0×104 NIH-3T3 fibroblasts were seeded onto 24-well plates and cultured in 10 % FCS/ DMEM (Gibco). Cells were treated for 60 min (at 37 °C and 50 rpm ...
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bioRxiv - Cell Biology 2024Quote: ... The absorbance of wells was subsequently measured at 450 nm using a Multiskan FC microplate reader (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: HeLa cells were cultured in Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) with Glutmax and 5% FCS (Gibco) and passaged every few days ...
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bioRxiv - Microbiology 2024Quote: Growth was assessed by measuring absorbance at 595 nm over time in a Multiskan FC microplate photometer (Thermo Scientific) using flat-bottom 96-well plates (Bioké ...
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bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 647-conjugated anti-Fc Fab (Surface protein and CYpHER quantitation, ThermoFisher Zenon Labeling Kits, mouse IgG2a for anti-EGFR 199.12 quantitation [ThermoFisher Z25108] ...
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bioRxiv - Cell Biology 2023Quote: Kidneys were harvested and transferred to MEM with Earle’s Salts (with 2.2 g/l NaHCO3, without L-Glutamine) medium (Biochrom) supplemented with 10% FCS (Life Technologies), 1x Non-Essential Amino Acids (NEAA ...
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bioRxiv - Molecular Biology 2023Quote: A549 cells were seeded in a 96-well black clear bottom plate at 2.5 × 104 cells/well in DMEM (10% FCS, 1% PenStrep (Gibco)) and cultured at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: A549 cells were seeded in a 96-well black clear bottom plate at 2.5 × 104 cells/well in DMEM (10% FCS, 1% PenStrep (Gibco)) and cultured at 37°C ...
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bioRxiv - Microbiology 2023Quote: HeLa and HEK293T cells were grown in DMEM (Dulbecco’s modified Eagle’s medium) with GlutaMAX and 10% decomplemented-FCS (fetal calf serum) (Gibco, Life Technologies). HeLa WT and HeLa BST2 KO cells were a kind gift of Stéphane Fremont (Institut Pasteur ...
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bioRxiv - Immunology 2023Quote: All single cell suspensions were prepared in Hank’s buffer (1×HBSS, 5% FCS, 10mM HEPES; all ThermoFisher, Life Technologies). For this ...
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bioRxiv - Immunology 2023Quote: All single cell suspensions were prepared in Hank’s buffer (1×HBSS, 5% FCS, 10mM HEPES; all ThermoFisher, Life Technologies). For this ...
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bioRxiv - Biochemistry 2023Quote: ... The established stable cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Nacalai Tesque) supplemented with 4∼5% fetal calf serum (FCS; Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... autoclaved and then supplemented with 0.2 to 1 mL (10% final) of fetal calf serum (FCS, Gibco Thermo Fisher); and 0.2-1 mL (10% final ...
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bioRxiv - Microbiology 2023Quote: ... autoclaved and then supplemented with 0.2 to 1 mL (10% final) of fetal calf serum (FCS, Gibco Thermo Fisher); and 0.2-1 mL (10% final ...
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bioRxiv - Immunology 2023Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 µL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... the remaining cells were stained for sorting in PBS containing 0.5% FCS at 4L°C with the following antibodies: Streptavidin-AF488 (1:100, Invitrogen), CD45-BV510(1:50 ...
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bioRxiv - Molecular Biology 2023Quote: A549 cells were seeded in a 96-well black clear bottom plate at 2.5 × 104 cells/well in DMEM (10% FCS, 1% PenStrep (Gibco)) and cultured at 37°C ...
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bioRxiv - Immunology 2023Quote: ... the corresponding cells were grown to near confluency (cultured in DMEM with 10% FCS (Benchmark) and 1% PSG (Gibco)) and harvested using 0.05% Trypsin-EDTA (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... A459 cells were grown in RPMI 1640 with L-glutamine and 10% heat-inactivated fetal calf serum (FCS, Gibco). Penicillin and streptomycin were added for routine culture but were removed the day before and during experiments.
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bioRxiv - Immunology 2024Quote: ... final volume of 200 µl RPMI 1640 containing 10% FCS and penicillin/streptomycin and 55 µM β-mercaptoethanol (Gibco) for the stated time periods in the presence of a range of [4Y]-MBP peptide concentrations ...