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Citations for Thermo Fisher :
3501 - 3550 of 5129 citations for SPFH1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The searched results were filtered with a 1% false discovery rate and the detected peptides were confirmed by manual analysis for monoisotopic mass accuracy and retention time with Xcalibur 2.2 (ThermoFisher). Fragment ions were also manually confirmed and were accepted based on the presence of a monoisotopic mass within 20 ppm mass accuracy ...
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bioRxiv - Cancer Biology 2023Quote: ... peptides were directly sprayed into an Orbitrap Fusion Lumos mass spectrometer equipped with a nanoFlex ion source (ThermoFisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Peptides were separated using a 140 min gradient generated by an EASY-nLC 1200 liquid chromatography (Thermo Fisher Scientific) heated to 50 °C by a PRSO-V1 column oven (Sonation) ...
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bioRxiv - Cell Biology 2023Quote: Samples were pooled in equimolar ratio and subjected to High pH Reversed-Phase Peptide Fractionation kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Dissolved peptides were added to Fe-NTA Magnetic Agarose (80 µL of suspension used; Cat # A52284, Thermo Fisher Scientific) washed with 80% ACN in 0.1% TFA and mixed for 60 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... approximately 1 μg of peptides were loaded on a C18 precolumn (PepMap100, 5 μm, 300 Å, Thermo Fisher Scientific) and separated over a C18 column (50 cm × 75 μm ...
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bioRxiv - Molecular Biology 2023Quote: ... The trapped peptides were separated on an Acclaim PepMap RSLC nanoViper column (75µm x 500mm, C-18, 2µm, 100Å, Thermo Fisher) at 300 nL/min flow rate and 40 °C column temperature using water and acetonitrile with 0.1% formic acid as solvents A and B ...
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bioRxiv - Neuroscience 2023Quote: Fe-NTA enrichment was carried out with a High Select Fe-NTA phospho-peptide enrichment kit (Thermo Fisher Scientific), as previously described29,30 ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were separated using a 140 min gradient generated by an EASY-nLC 1200 liquid chromatography (Thermo Fisher Scientific) heated to 50 °C by a PRSO-V1 column oven (Sonation) ...
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bioRxiv - Cell Biology 2023Quote: ... HDFs were cultured for 72 hours in the presence of each peptide (at 3-12.5 ppm)in Dulbecco’s modified Eagle’s medium (DMEM, Gibco #21969-035) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... peptides were first loaded onto a trap cartridge (ThermoFisher PepMap, C18, 5 μm, 100 μm i.d. x 20 mm), then eluted onto a reversed phase Easy-Spray column (ThermoFisher PepMap ...
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bioRxiv - Systems Biology 2023Quote: ... with 2 μm bead size was used for peptide separation in an EASY-nLC 1200 HPLC (Thermo Fisher Scientific). Peptides were separated in a gradient composed as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted peptides were sprayed directly by electrospray ionization into a Q Exactive Plus Orbitrap mass spectrometer (Thermo Scientific). Mass spectrometry measurement was conducted in data-dependent acquisition mode using a top10 method with one full scan (mass range ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were then separated (EASY-Spray; PepMap RSLC C18, 75 μm × 50 cm, 2 μm particles, Thermo Fisher Scientific) at a flow rate of 250 ηL min-1 employing a two-step gradient ...
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bioRxiv - Neuroscience 2023Quote: ... was used to elute peptides from the capillary reverse-phase column (0.075 × 150 mm, Pepmap®, Thermo Fisher Scientific). Data were acquired using the Xcalibur software ...
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bioRxiv - Biochemistry 2023Quote: We labeled 10 µg of tryptic peptides from each experiment with a TMT10plex Isobaric Labeling Reagent kit (Thermo Scientific) (Zecha et al. ...
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bioRxiv - Systems Biology 2023Quote: ... the dried peptides were dissolved in 250 µL of buffer A (0.1% formic acid in H2O) (Thermo Fisher Scientific) with 1:2500 (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... Chromatographic separation of the peptides was performed by liquid chromatography on a nano-HPLC system (Ultimate 3000, Thermo Scientific) using a nano-LC column (Acclaim PepMapC100 C18 ...
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bioRxiv - Cell Biology 2023Quote: ... An aliquot containing ∼ 100 µg of peptides was removed and labeled with Tandem-Mass-Tag (TMT) reagent (ThermoFisher Scientific). Once labeling efficiency was confirmed to be at least 95% ...
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bioRxiv - Microbiology 2023Quote: ... and tryptic peptides were cleaned using a C18 column and dried with a SpeedVac concentrator (Thermo Fisher Scientific, USA). The peptides were quantified using the Pierce Quantitative Colorimetric Peptide Assay Kit ...
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bioRxiv - Microbiology 2023Quote: ... USA).The resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific, Rockford, IL, USA) at the Vienna Research Platform for Metabolomics & Proteomics and analyzed using the Proteome Discoverer v2.2.0.388 (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... Final peptide concentration was estimated by measuring absorbance at 280 nm on a NanoDrop 2000C spectrophotometer (Thermo Fisher Scientific). The resulting peptide mixtures from yeast were desalted by Stage-tips ...
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bioRxiv - Immunology 2023Quote: ... LC/MS data acquisition parameters: Peptides were separated and analyzed on an EASY nLC 1200 System (Thermo Fisher Scientific) in-line with the Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo Fisher Scientific ...
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Pan-tissue mitochondrial phenotyping reveals lower OXPHOS expression and function across tumor typesbioRxiv - Biochemistry 2023Quote: ... The multiplexed peptide sample was subjected to high pH-reversed-phase fractionation according to the manufacturer’s instructions (Thermo Fisher). In this protocol ...
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bioRxiv - Immunology 2023Quote: ... Separation of peptides was achieved using a DNV PepMap Neo separation column (75 µm x 150 mm, Thermo Scientific) and a gradient from 1 % to 40 % B within 120 min and a flowrate of 400 nL/min at 60 °C ...
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bioRxiv - Immunology 2023Quote: ... LC/MS data acquisition parameters: Peptides were separated and analyzed on an EASY nLC 1200 System (Thermo Fisher Scientific) in-line with the Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were gradient eluted from the column directly to Eclipse mass spectrometer using a 1 hour gradient (Thermo Scientific) using 2% acetonitrile and 0.5% acetic acid for solvent A and 80% acetonitrile and 0.5% acetic acid for solvent B.
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bioRxiv - Molecular Biology 2023Quote: ... applying settings from the literature for analyzing HLA peptides (Bassani-Sternberg et al. 2015) and ProteomeDiscoverer 2.3 software (Thermofisher). Raw data has been deposited to ProteomeXchange Consortium via the MassIVE partner repository with data set identifier PXD042439.
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... Each peptide extracts were loaded on a 300 µm ID x 5 mm PepMap C18 precolumn (Thermo Scientific, USA) at a flow rate of 10 µL/min ...
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bioRxiv - Microbiology 2023Quote: ... 70 ng of peptides according to the results of the Invitrogen Qubit protein assay (Thermo Fisher Scientific, Darmstadt, Germany) supplemented with 40 fmol of Hi3 Phos B standard for protein absolute quantification (Waters ...
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bioRxiv - Immunology 2023Quote: Peptide analysis using data-dependent mass spectrometry (DDA-MS) was performed on a Q Exactive HFX instrument (Thermo Scientific) connected to an Easy-nLC 1200 system (Thermo Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Peptides eluted from the column were introduced into a Finnigan LTQ-Obitrap Elite hybrid mass spectrometer (Thermo Scientific TM) with electrospray ion source setting at 2.5 kV ...
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bioRxiv - Microbiology 2023Quote: ... Peptide separation was carried out using an Acclaim PepMap RSLC C18 column (75 μm × 50 cm; Thermo Fisher Scientific) using standard reverse-phase gradients ...
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bioRxiv - Cancer Biology 2023Quote: ... Tryptic peptides were loaded onto a trap column (Pepmap 100 5μm, 5 × 0.3 mm, ThermoFisher Scientific, San Jose, CA) at a flow rate of 10 μL/min using 0.1% TFA as loading buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were identified using an ACQUITY M-Class UHPLC system (Waters) connected to an Orbitrap Elite mass spectrometer (ThermoFisher). Approximately 1 µg of samples was injected onto an ACUITY UPCL M-Class Symmetry C18 Trap Column ...
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bioRxiv - Biochemistry 2023Quote: ... The peptides were first concentrated on a precolumn (PepMap100 C18 3 μm; 75 μm × 2 cm; Thermo Fisher Scientific) and then separated on an EASY-Spray column (ES903 ...
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bioRxiv - Biochemistry 2023Quote: Tryptic peptides were analyzed by LC-MSMS using a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen, Germany). Peptide mixtures were fractionated by an Ultimate 3000 RSLCnano (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: The peptide mixture from the biological replicates was analyzed by LTQ Velos Orbitrap mass spectrometer (Thermo Fisher Scientific, USA) coupled with liquid chromatography-tandem mass spectrometry using an EASYnLC system (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2023Quote: ... Peptides were then vacuum dried and concentration was determined by measuring the absorbance at 280/260 (NanoDrop, Thermo Scientific). 200μg of peptides in 100mM HEPES pH=8-5 were then labeled with 400µg of 11-plex TMT (Thermo ...
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bioRxiv - Cell Biology 2023Quote: ... All native peptides not captured by the phosphopeptide enrichment kits were then separated by offline medium pH C4 peptide fractionation (Accucore 150-C4, 2.6um pore size, 150mm X 2.1mm, Thermo Scientific) using gradient mobile phase conditions as previously reported (76) ...
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bioRxiv - Cell Biology 2024Quote: ... peptides were resolved using an EASY-Spray capillary HPLC column (ES902A, 75 µm × 25 µm, 100Å, 2μm, Thermo Scientific) and a Vanquish Neo UHPLC (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Peptides were loaded onto an Acclaim PepMap100 nanoViper C18 trap column (100 µm inner diameter, 2 cm; Thermo Scientific) in HPLC buffer C with a constant flow of 10 µl/min ...
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bioRxiv - Neuroscience 2024Quote: Fractionated peptides were analysed by LC-MS/MS using a fully automated Ultimate 3000 RSLC nano System (Thermo Scientific). Peptides were trapped with a PepMap100 C18 5 μm 0.3X5 mm nano trap column (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2024Quote: ... Peptides were separated on a 75 µm × 50 cm EASY-Spray C18 column (2 µm, 100 Å; Thermo Scientific) at 50 °C using a linear gradient from 10% to 40% B in 153 min with a flow rate of 200 nL/min ...
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bioRxiv - Immunology 2024Quote: ... injections into both hind flanks of 0.5 μmol/mL MOG35-55 peptide emulsified in incomplete Freuds adjuvant (Cat# 77145; ThermoFisher) supplemented with 4 mg/mL heat-killed M ...
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bioRxiv - Physiology 2024Quote: ... Peptides were loaded in buffer A (0.1 % formic acid) onto a 110 cm mPAC HPLC column (Thermo Fisher Scientific) and separated with a non-linear gradient of 1 – 50 % buffer B (0.1 % formic acid ...
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bioRxiv - Cancer Biology 2024Quote: ... reconstituted peptides were analysed on a Dionex Ultimate 3000 system coupled with the nano-ESI Fusion Lumos (Thermo Scientific). Peptides were loaded on the Acclaim PepMap 100 ...
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bioRxiv - Plant Biology 2024Quote: ... Approximatively 200ng of each peptide sample were concentrated onto a C18 pepmap 100 (5mm x 300µm i.d.) precolumn (Thermo Scientific) and separated at 50°C onto a reversed phase Reprosil column (25cm x 75μm i.d. ...
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bioRxiv - Cancer Biology 2024Quote: ... Each peptide extracts were loaded on a 300 µm ID x 5 mm PepMap C18 precolumn (Thermo Scientific, USA) at a flow rate of 20 µL/min ...
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bioRxiv - Biochemistry 2024Quote: ... approximately 100 nanograms of peptide were analyzed by LC-MS/MS with an EASY-nLC 1200 (Thermo Fisher Scientific) and an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific ...