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Citations for Thermo Fisher :
3501 - 3550 of 10000+ citations for Recombinant Mouse Bace1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cells at the end of 1-day culture were pulsed for 20 minutes with 40 ng/ml of recombinant human IFN-γ (Gibco/Thermo Fisher), 100 IU/ml of recombinant human IL-2 (Roche Diagnostics ...
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bioRxiv - Cancer Biology 2023Quote: ... Single guide RNAs (sgRNAs) were incubated together with recombinant Cas9 (TrueCut Cas9 Protein v2, 5ug/ul, Thermo Fisher Scientific, cat. no. A36499) for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... were cultured in in K-SFM + Human recombinant Epidermal Growth Factor (rEGF) and Bovine Pituitary Extract (BPE) supplemented with 1% penicillin and streptomycin (all from Thermo Fisher Scientific). PCa cell line PC-3 (CRL-1435) ...
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bioRxiv - Genomics 2023Quote: ... Integrity of the GST tag was subsequently checked via western blotting of the purified recombinant protein using anti-GST antibodies (Invitrogen 71-7500) [1:1,500 dilution] ...
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bioRxiv - Cell Biology 2024Quote: ... and 33 µl of this buffer was added to the beads together with 10 µl Recombinant Proteinase K Solution (20 mg/ml, Thermo Fisher, AM2548) and 3 µl RiboLock RNase inhibitor (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... AcGFP-JEVcore recombinant protein and Alexa Fluor 594 or 555-conjugated antibody (2 mg/mL, Thermo Fisher Scientific, as an injection marker) were microinjected into the cytoplasm at a final concentration of 1 mg/mL ...
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bioRxiv - Biochemistry 2023Quote: ... Single colonies were picked and cultured overnight in LB medium + 50 µg/mL kanamycin and recombinant plasmids isolated using the GeneJET Plasmid Miniprep Kit (Thermo Fisher #K0503). Insert sequences in each plasmid were confirmed by Sanger sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... DD5A)29 and three controls (Ctl1B, Ctl7C, Ctl8F)47 were cultured on human recombinant laminin LN521 (Biolamina) in Essential-8TM medium (Thermo Fisher Scientific) and passaged using Trypl Express (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... the microtiter plates were washed 5 × with T-PBS and then incubated for 1 hour at room temperature with 2 μg/ml of biotinylated recombinant protein G (PierceTM, Thermo Fisher Scientific) for recognition of IgG antibodies from human ...
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bioRxiv - Neuroscience 2024Quote: ... Phosphorylation was induced by incubating 1 µg of recombinant human polo-like kinase 3 (PLK3) protein (Thermo Fisher Scientific, MA, USA; #PR7316B) with 50 µL of mouse WT α-synuclein in kinase reaction buffer for 8 hrs at 30°C ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 construct was tagged on the N-terminus with 10xHis and MBP tag cleavable by TEV protease ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA for human HDAC1 with C-terminal FLAG tag was cloned into the pFastBac donor vector using standard PCR-based site-directed mutagenesis techniques and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). Detection of gp64 was used to determine baculovirus titer (Expression Systems) ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 protein was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 construct was tagged on the N-terminus with 6×His cleavable by TEV protease ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 constructs were tagged on the N-terminus with 10xHis and MBP tag cleavable by TEV protease ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNAs for human KBTBD4 was cloned into the pFastBac donor vector and the recombinant baculovirus was constructed using the Bac-to-Bac protocol and reagents (Thermo Fisher Scientific). KBTBD4 medulloblastoma mutations were introduced into coding regions using standard PCR-based site-directed mutagenesis techniques ...
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bioRxiv - Genetics 2019Quote: ... Thirty-two libraries of 100 pM were mixed together and emulsion PCR amplified on the Ion PI™ Hi-Q™ ISPs using the Ion OneTouch™ 2 Instrument (Life Technologies, Carlsbad, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Template preparation was performed by the Ion PGM Hi-Q View kit on the Ion OneTouch™ 2 System (Life Technologies, Grand Island, NY, United States) and sequenced using the Ion PGM Hi-Q View sequencing kit (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... in HepG2 medium (Advanced MEM minus L-Glutamine [Gibco] + 10% [v/v] HI-FBS [Gibco] + 1% [v/v] penicillin-streptomycin + 2 mM Glutamax [Gibco] and 0.1% [v/v] amphotericin B). Once the HepG2 or HC-04 cells were grown to the desired concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary (anti-flag mouse 1:200, Cell Signalling) and secondary antibody (anti-mouse Alexa 488 1:500, Thermofisher) were diluted in blocking buffer solution and incubated overnight at 4°C or 1 hour at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary anti-mouse antibody conjugated to HRP (goat anti-mouse-HRP, 32430; ThermoFisher Scientific) at 1:20,000 in 5% milk/1xTBST for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... or with unconjugated 4.3.1.3 mouse MAb and secondary detection with goat anti-mouse Alexa-Fluor 647 (A-21235; Life Technologies). Immunofluorescence was used to boost GFP signal in TgBAC(lck:gfp)vcc6 specimens with a chicken anti-GFP primary (Abcam ...
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bioRxiv - Neuroscience 2022Quote: Total mouse tau ELISA measurements were carried out using a total mouse tau ELISA kit (Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2019Quote: Cells were obtained from mouse spinal cord and/or cultured mouse embryonic fibroblasts resuspended in Trizol (Thermo Fisher). RNA was extracted using miRNeasy Mini Kit (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... mouse anti-human IgG3 (HP6050) or mouse anti-human IgM (clone HP6083) at 1/1000 (Thermo Fisher Scientific) for 1 hour at RT ...
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bioRxiv - Genomics 2022Quote: ... anti-mouse 800 (only used in Figure 8E,F to detect mouse anti- FLAG) (1:5000, Invitrogen A32730). Anti-Sloth1 and Anti-Sloth2 antibodies (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... and with LIN (anti-mouse TER119-FITC, Thermo Fisher, 11-5921-82; anti-mouse CD31-FITC, Thermo Fisher, 11-0311-85 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Total RNA was extracted from mouse skin tissue or mouse/human EpSCs using Trizol reagent (Invitrogen, Massachusetts, USA), and used as a template for cDNA synthesize using HiScript II reverse transcriptase (Vazyme Biotech ...
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bioRxiv - Immunology 2023Quote: ... The secondary antibody reactive to mouse IgG (anti-Mouse IgG (H+L)-AF647) was purchased from Thermo Fisher Scientific.
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bioRxiv - Neuroscience 2023Quote: ... goat anti-mouse IgG Alexa546-labeled monoclonal antibody goat anti-mouse IgG Alexa633-labeled monoclonal antibody (ThermoFisher Scientific); DAPI ...
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bioRxiv - Microbiology 2023Quote: ... mouse primary antibody was visualised with 1:1000 Donkey α Mouse Alexa Fluor™ Plus 647 (Invitrogen, A32787) or 1:1000 Donkey α Mouse Alexa Fluor™ Plus 555 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: CLP was performed on WT and Plk3−/− mice following retro orbital injection (0.1μg/g mouse) of anti-mouse GPIX (Emfret) conjugated to AlexaFluor-750 (ThermoFisher) as previously described 21 ...
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bioRxiv - Neuroscience 2024Quote: ... or RGS14 (n=3, 1:500, mouse anti-RGS14 #75-170, Neuromab)/goat anti-mouse IgG2a (Life Technologies).
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bioRxiv - Immunology 2024Quote: ... mouse anti-human IgG3 (HP6050) or mouse anti-human IgM (clone HP6083) at 1/1000 (Thermo Fisher Scientific) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-KRT 14 (1:10, Invitrogen); rabbit anti-KRT 19 (1:10 ...
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bioRxiv - Cancer Biology 2021Quote: ... and Mouse anti-CD31 (ThermoFisher; 1:100). Secondary antibodies were Goat and Donkey anti-IgG and usually either Alexa Fluor-488 ...
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bioRxiv - Cancer Biology 2021Quote: ... Goat-α-mouse-Alexa-488 (A28175, Invitrogen) was used as the secondary antibody ...
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bioRxiv - Cell Biology 2019Quote: ... and Alexa Fluor 488 anti mouse (Thermofisher) diluted 1/500 in PBS-BSA 1% and 0.4 U.mL-1 RNAsin (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... and mouse anti-Pgk1 (22C5D8, Molecular Probes) antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-SSEA4 (1:100, Life Technologies); rat anti- SOX2 (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated goat anti-mouse IgG (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: Mouse anti-c-Myc (MA1-980, Thermofisher) and Biotin anti-IL-1β (511703 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse anti-αII-spectrin (1:200, Invitrogen), rabbit anti-β-actin (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-occludin (1/500, 331500, Invitrogen), mouse anti-E-cadherin (1/500 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-TfR (1:100, Invitrogen 905963A), mouse anti-Ubiquitin (1:25 ...
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bioRxiv - Cell Biology 2020Quote: ... CDK5 mouse monoclonal (Invitrogen, cat. no. AHZ0492) (1:100) ...
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bioRxiv - Cell Biology 2020Quote: ... mouse p21 (1:1000, Invitrogen, ma5-14353), rabbit p19 (1:200 ...
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bioRxiv - Genetics 2021Quote: ... mouse anti-GFP (Invitrogen, A11120; 1:300), rat anti-HA (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... monoclonal mouse OTX2 antibody (MA5-15854, Invitrogen); (7 ...
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bioRxiv - Biochemistry 2020Quote: ... Mouse anti-CPY (1:5,000; Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse-546 (Thermo Fisher Scientific #A-11030) and GP-647 (Thermo Fisher Scientific #A-21450).