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Citations for Thermo Fisher :
3501 - 3550 of 10000+ citations for Recombinant Human TNFSF18 Protein Fc Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... The biotinylated primers and undigested library members were removed using Dynabeads M-270 Streptavidin (Thermo Fisher Scientific, Waltham, MA), using the recommended “Immobilize nucleic acids” protocol ...
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bioRxiv - Bioengineering 2019Quote: ... was biotinylated using a 5:1 molar excess of Ez-Link™ Sulfo-NHS-LC-Biotin (Thermo-Fisher Scientific) overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: The input (total Nav1.5 expression) and biotinylated (cell surface-expressed Nav1.5) samples were separated on NuPAGE 4–12% Bis-Tris gels (Invitrogen, ThermoFisher) and transferred to nitrocellulose membrane (iBlot transfer system ...
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bioRxiv - Molecular Biology 2021Quote: ... Biotinylated RNA was purified also as described (Duffy and Simon, 2016) using 80 μl MyOne Streptavidin C1 Dynabeads (Invitrogen) + 100 μl biotinylated RNA for 15 min at RT with rotation and under foil ...
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bioRxiv - Bioengineering 2019Quote: ... the prepared bacterial suspension was incubated with 10 µg/mL biotinylated Salmonella polyclonal antibody (Thermo Scientific, Waltham, MA, USA) on a vortex mixer at 500 rpm at room temperature for 1 hr ...
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bioRxiv - Immunology 2020Quote: ... S-specific B cells were identified using biotinylated SARS-CoV-2 S probe conjugated to Streptavidin-PE (Thermo Fisher). Streptavidin-BV510 (BD ...
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bioRxiv - Genomics 2022Quote: Biotinylated informative DNA ligation products were pulled down using Dynabeads MyOne streptavidin C1 paramagnetic beads (Thermo Fisher cat. #65001). After thorough washing of the ligation products-beads complex and having the sample in 35.7μl of volume ...
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bioRxiv - Synthetic Biology 2022Quote: The RBDs were biotinylated by amine coupling with sulfo-NHS-SS-Biotin according to the manufacturer’s manual (Thermo Fisher). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... the concentration of biotinylated Igs after desalting was measured at 280 nm via spectrophotometer (Nanodrop ND-1000, ThermoFisher Scientific); biotin incorporation was measured using the Pierce Biotin quantitation kit (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... 5303 SPRN SPRN-5) was biotinylated using the EZ-Link NHS-PEG4 Biotinylation Kit (Thermo Scientific, catalog No. PI21455) and used at a concentration of 1µg/ml ...
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bioRxiv - Microbiology 2024Quote: ... both RBD and HEL were site-specifically biotinylated in house using EZ-link maleimide-PEG2-biotin (ThermoFisher, CAT#A39261) so that the binding epitopes on these proteins were not modified ...
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bioRxiv - Molecular Biology 2023Quote: ... an amino acid analog of methionine containing an alkyne moiety that can be biotinylated by click-it chemistry (ThermoFisher), for nascent protein translation labeling followed by SDS-PAGE and HRP-streptavidin Western analysis ...
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bioRxiv - Genomics 2023Quote: ... Biotinylated RNA was purified also as described (Duffy and Simon, 2016) using 80 μl MyOne Streptavidin C1 Dynabeads (Invitrogen) + 100 μl biotinylated RNA for 15 min at RT with rotation and under foil ...
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bioRxiv - Neuroscience 2023Quote: ... Biotinylated hSA4 antibody (in-house antibody) was generated using 200 μg Sulfo-NHS-LC Biotin (Pierce, Thermo Fisher Scientific), diluted 1:200 in blocking buffer and added to the plate for 1 hour at 37 °C ...
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bioRxiv - Physiology 2024Quote: ... sections were washed 3 times with PBS and incubated at room temperature with biotinylated universal secondary antibody (Life technologies). After washing with PBS x3 ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated IgG retained on plates was reacted with a 1:10,000 dilution Streptavidin-HRP conjugate (Thermo Scientific, cat. N504) in ADA-WB containing 10 mg/ml BSA for one hour at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Stromal cells were suspended in DMEM: F12 Nutrient Mixture (Hyclone) containing 10% heat-inactivated FCS (Gibco), 50 units/mL penicillin ...
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bioRxiv - Immunology 2019Quote: ... Optic density was then measured at 405 nm on a Multiskan FC microplate photometer (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... Digestion was stopped with RP-HE (RPMI 1640, Thermo Fisher; 10% FCS (Corning, #35-079-CV), 10 mM EDTA (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... The plates were read with a Multiskan FC microplate reader (Thermo Fisher Scientific Inc., Waltham, MA) at 570 nm ...
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bioRxiv - Biochemistry 2020Quote: ... An in-frame fusion between ACE2 and Fc was constructed in pcDNA3 vector (Invitrogen, Carlsbad, CA). Site-directed mutagenesis by PCR was performed to create the panel of ACE2-Fc mutants ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial cell density was measured as an optical density (OD, 595 nm; Multiscan FC Thermo Scientific) and colony forming units per ml (CFU/ml ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with final 10 % (v/v) fetal calf serum (FCS, Thermo Fisher Scientific, Waltham, MA, USA), 1x non-essential amino acids (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Biowest, Nuaillé, France) and antibiotics (Gibco, Waltham, MA, USA). The human NSCLC cell line NCI-H23 (H23 ...
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bioRxiv - Cell Biology 2021Quote: ... in Ham’s F10 medium supplemented with 10% dialyzed FCS and 20 mM HEPES buffer (both Gibco). Cells were labeled for 30 minutes using 400 µM EU (MRC-5 cells ...
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bioRxiv - Biophysics 2020Quote: ... ACE2-Fc was purified to homogeneity from the conditioned medium using PoRos XQ column (Thermo Fisher) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... S2R+ Cells (2 × 105) were seeded in Schneider medium plus 10% FCS (Gibco 21720024, Sigma F9665) in a 24-well plate ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10 % fetal calf serum (FCS) (Biochrom, Ltd., Berlin, Germany) and 1 % penicillin-streptomycin (Invitrogen). The human colorectal adenocarcinoma cell line Caco-2 was provided by Jindrich Cinatl Jr ...
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bioRxiv - Microbiology 2022Quote: THP-1 cells were diluted to 5 x 105 cells/mL in RPMI + 10% FCS (Gibco) and treated with 100 nM phorbol 12-myrystate-13-acetate (PMA ...
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bioRxiv - Immunology 2020Quote: Single cell preparations were pre-incubated with Fc-Block (eBioscience, Thermo Fisher Scientific, Les Ulis, France) for 20 min at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... and absorbance were subsequently measured at 450 nm using a Multiskan™ FC photometer (Thermo Fisher).
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with anti-CD16/32 (Fc Block) antibodies and Fixable Live/Dead stain (Invitrogen) for 15 min before staining with antibodies for surface markers in 2% BSA/PBS buffer for 30 mins on ice ...
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bioRxiv - Immunology 2019Quote: ... Cells were cultured in DMEM (Wisent) or RPMI1640 supplemented with 10% FCS and penicillin/streptomycin (Gibco). Media were supplemented with 200 UI/mL IL-2 (Proleukin – Novartis Pharmaceuticals ...
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bioRxiv - Biophysics 2019Quote: ... supplemented with 10% (v/v) heat-inactivated Hyclone fetal calf serum (FCS; Thermo Scientific, UT, USA), 2mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 20% fetal calf serum (FCS) and 0.5% gentamycin (all Gibco Life Technologies, Carlsbad, USA), grown at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 20% fetal calf serum (FCS) and 0.5% gentamycin (all Gibco Life Technologies, Carlsbad, USA), grown at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: Mouse bone marrow cells were isolated by crushing entire long bones into DMEM/3% FCS (GIBCO). Bone fragments were removed by filtration through a 70 μm filter mesh ...
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bioRxiv - Microbiology 2020Quote: COS7 and HEK293T cells were maintained in DMEM supplemented with 10 % FCS and GlutaMAX (Life Technologies), 5 % CO2 ...
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bioRxiv - Immunology 2021Quote: ... washed in TBS and blocked with StartingBlock T20 Blocking buffer containing Fc blocker (Thermo Scientific, 37543). Sections were incubated with unconjugated L ...
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bioRxiv - Immunology 2021Quote: ... mice were directly perfused with PBS/ 2 % FCS/ 1 mM EDTA and liver digest medium (ThermoFisher Scientific/ Gibco ...
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bioRxiv - Immunology 2021Quote: ... The optical density was measured at 450 nm with a Multiscan FC Plate Reader (Thermo Scientific).
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bioRxiv - Immunology 2021Quote: ... was measured as absorbance at 450 nm (OD450) by Spectrophotometer (Thermo Scientific, Multiskan FC Microplate Photometer). The titers were defined from the reciprocal value of the absorbance at OD450.
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bioRxiv - Immunology 2020Quote: ... ACE2-Fc was purified to homogeneity from the conditioned medium using PoRos XQ column (Thermo Fisher) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: Cells were then subjected to Fc blockade with anti-CD16/CD32 (Thermo Fisher Scientific, Waltham, MA), washed with 1XPBS ...
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bioRxiv - Immunology 2022Quote: ... The isolated lungs were placed in RPMI-1640 containing 2% (vol/vol) FCS (Thermo Fisher Scientific), 50 μg/ml Liberace TM (Roche ...
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bioRxiv - Immunology 2022Quote: ... explanted organs were stored in 600 μL DMEM containing 10% FCS and 1% Penicillin/Streptomycin (Gibco). After mechanical disruption by shaking at 25/s over 4 min with metal beads (TissueLyser II ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10% (vol/vol) fetal calf serum (FCS, Biochrom) and antibiotics (1x Pen/Strep, Gibco). tRF were generated from primary rhesus fibroblasts (RF ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were cultivated in DMEM-F12 medium containing 20% fetal bovine serum (FCS, Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: MetR medium was prepared by titrating 0% MetR medium (complete DMEM, ThermoFisher, 21013-024, 10% FCS) with 100% MetR medium (complete DMEM without L-methionine and L-cysteine ...
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bioRxiv - Systems Biology 2023Quote: ... cells were washed and maintained in regular culture medium (DMEM GlutaMAX™, Gibco®, 10% FCS) for 5 days ...