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Citations for Thermo Fisher :
3501 - 3550 of 10000+ citations for Mouse Anti MERS Coronavirus Spike S1 Antibody 3873 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Slides were fixed with 4% paraformaldehyde for 10 min before addition of anti-rat Alexa Fluor 555 and anti-mouse Alexa Fluor 488 secondary antibodies (Thermo Fisher Scientific). Fluorescently-labelled DNA fibers were imaged using a Nikon Eclipse Ni microscope with NIS-Elements software (Nikon Instruments) ...
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bioRxiv - Biophysics 2019Quote: ... The cells were then washed and incubated for 70 minutes at room temperature with Alexa 488/561-conjugated anti-rabbit and anti-mouse secondary antibodies (Molecular Probes, Invitrogen). The double labeled cells were then washed and kept a 4° C until confocal imaging ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies were: Alexa-488 goat anti-mouse IgG and Cy3 goat anti-rabbit IgG (both 1:100 dilution; ThermoFisher, Leicester, UK). Brains were viewed using an Olympus FV1000 confocal microscope ...
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bioRxiv - Cell Biology 2019Quote: ... For the negative control primary antibodies mouse anti-plasmepsin V (From D. Goldberg, 1:1) and rabbit anti-GFP A-6455 (Invitrogen, 1:200) were used.
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bioRxiv - Genomics 2021Quote: ... Appropriate secondary antibody (goat anti-mouse-Alexa-Fluor-488, goat anti-rabbit-Alexa-Fluor-488 or 568, 1:500; Invitrogen, Carlsbad, CA) was used ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were washed 3x 5 minutes in PBST then incubated with anti-rabbit and anti-mouse antibodies (LI-COR, 1:15,000 or ThermoFisher, 1:10,000) diluted in 4% milk/PBST for 1.5 h at RT ...
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bioRxiv - Biochemistry 2021Quote: ... and secondary antibodies were goat anti-mouse Alexa Fluor 596 and goat anti-rabbit Alexa Fluor 488 (both ThermoFisher Scientific; 1:1000).
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bioRxiv - Biochemistry 2021Quote: ... and secondary antibodies were goat anti-mouse Alexa Fluor 596 and goat anti-rabbit Alexa Fluor 488 (both ThermoFisher Scientific; 1:1000).
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bioRxiv - Immunology 2021Quote: ... Secondary antibodies included goat anti-mouse Alexa Fluor-488 or -568 and goat anti-rabbit Alexa Fluor-488 or -568 (Thermo Fisher Scientific). Western blots were processed using the iBind Flex device (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed with PBS-Tx and incubated with secondary antibodies (Alexa Fluor 488 donkey anti-mouse, 1:500, Life Technologies; Alexa Fluor 555 donkey anti-rabbit, 1:500, Fisher Scientific) for 2 h at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... were incubated with fluorescent secondary antibodies (Goat anti-mouse IgG DyLight 800 ThermoFisher #SA5-10176 and Goat anti-rat IgG DyLight 800 ThermoFisher #SA5-10024), and images acquired on a LI-COR Odyssey instrument ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were incubated overnight at 4°C with primary antibody and then washed three times with 2% BSA in PBS for 5 min each and incubated with Alexa Fluor 488- and/or 568-conjugated anti-mouse and/or anti-rabbit IgG secondary antibodies (1/500, Thermo Fisher Scientific) for 1 hr at RT ...
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bioRxiv - Cell Biology 2022Quote: ... -647-conjugated anti-mouse-IgG and anti-rabbit-IgG secondary antibodies (A32723, A32731, A11004, A11011, A11032, A11037, A21236, A21245; Thermo Fisher Scientific). For western blot analysis the following secondary antibodies were used ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were washed three times with PBS and incubated with Alexa Fluor anti-rabbit and anti-mouse secondary antibodies diluted in goat serum (respectively ref #A-21244 and #A-11017, Thermo Fisher Scientific) for 1h at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... Secondary antibodies: goat anti-rabbit or goat anti-mouse IgG2a conjugated to Alexa-647 (Thermo Fisher, A21245 and A21241 respectively, 1:400) and donkey anti-rabbit conjugated to Alexa-568 (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... washed for 24 hours and incubated in secondary antibodies (donkey anti-mouse 555, 1:1000, donkey anti-chicken 488, 1:1000, Invitrogen, A31570, A21202) for four days at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... Alexa Fluor secondary antibody (Goat anti-Rabbit IgG, Alexa Fluor 488, A-11034; Goat anti-Mouse IgG, Alexa Fluor 488, A-11029, Thermo Fisher Scientific) were used for double color detection ...
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bioRxiv - Physiology 2022Quote: ... Slides were then incubated in the dark for 1h at room temperature in secondary antibodies (1:500 Goat anti-Guinea Pig, Alexa Fluor® 594 and Goat anti-Mouse Alexa Fluor® 488, Invitrogen), washed 3 times for 5 minutes in PBST ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated with secondary antibodies (anti-mouse-Alexa Fluor 488 and anti-rat-Alexa Fluor 647) and NeutrAvidin-DyLight 594 (ThermoFisher Scientific 22842) in blocking buffer (10% goat serum ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated for 1 h with the secondary-antibody horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse (1:1000; Thermo Fisher Scientific) in 5% milk in TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... After washing, secondary antibodies (488 anti-mouse Jackson 715-545-151, 594 anti-rabbit Jackson 711- 585-152) and DAPI (Life Technologies D1306) were added at 1:1000 in PBST with 5% BSA overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... membranes were washed with 1% BSA/PBS and incubated with 1:500 dilution of secondary antibodies (goat anti-mouse or goat anti-rabbit) conjugated with Alexa Fluor-488 (Thermo Fisher Scientific) at RT for 1.5 h ...
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bioRxiv - Microbiology 2022Quote: ... The slides were then incubated with the corresponding secondary antibody solution (anti-mouse or anti-rabbit AF488 or AF647, Thermo Fisher Scientific) in 1x PBST for 2 hours at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... or 53BP1 clone 6B3E10 (Santa-Cruz) and visualized using Alexafluor-488-conjugated goat anti-rabbit and Alexfluor-546-conjugated goat anti-mouse antibodies (Invitrogen™, Life technologies) along with 4′,6-diamidino-2-phenylindole ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were washed with PBS three times for 5 min and incubated with secondary antibodies (goat anti-rabbit-AlexaFluor488: 1:1000, Thermo Fisher Scientific, Cat. #A-11034; goat anti-mouse-AlexaFluor488: 1:1000, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... sections were washed three times with PBS and incubated with Alexa 488 (labeled anti-mouse or rat, 1:400) or Alexa 594 (labeled anti-rabbit, 1:400)-conjugated secondary antibody (Invitrogen, Waltham, MA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were incubated with primary antibodies (Rabbit-anti-GFP: 1:1000, Thermo Fisher Scientific, Cat. #G10362; mouse-anti-c-myc: 1:200, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... each membrane was probed for using the following primary antibodies: mouse anti-CFTR 596 (1:1000) (UNC) or CAR anti-rabbit (CXADR) (1:100) (PA5-31175, Invitrogen, Waltham, MA) was applied for 2 hours ...
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bioRxiv - Bioengineering 2023Quote: ... the embryos were stained with secondary antibodies with Alexa 488 goat anti-rat IgG and Alexa 594 goat-anti-mouse IgG (1:250, Invitrogen, Carlsbad, CA) in blocking buffer overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were subsequently washed 3 x 5 mins with PBS and incubated in fluorescently conjugated secondary antibodies (goat anti-mouse Alexa Fluor 488, ThermoFisher #A11001, 1:1,000; goat anti-rabbit Alexa Fluor 546, ThermoFisher #A11010, 1:1,000) diluted into blocking buffer together with Phalloidin Alexa 647 (ThermoFisher #A22287 ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were washed with PBS and incubated with secondary antibody (goat anti-mouse Alexa Fluor 555, cat#A21422Invitrogen, 1:250; goat anti-rabbit Alexa Fluor 647, cat#21244, Invitrogen, 1:250) for overnight at 4°C under agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... Fluorophore tagged secondary antibody (Alexa Fluor 568 anti-mouse IgG of Invitogen, A-11004 and Alexa Fluor 488 anti-rabbit IgG of Invitrogen, A-11001) were used in 1:200 dilution and incubated for 1 hrs at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Donkey anti-mouse Alexa Fluor 488 and donkey anti-rabbit Alexa Fluor 594 secondary antibodies were purchased from Invitrogen (Carlsbag, CA, USA). Goat anti-mouse and goat anti-rabbit immunoglobulin-horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2023Quote: ... together at 4°C for 18 hours followed by appropriate secondary antibodies (donkey anti-mouse IgG [H+L]; A48257 and donkey anti-sheep IgG [H+L]; A11016; 1:1000; Invitrogen, Waltham, MA) for 1 h ...
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bioRxiv - Physiology 2023Quote: ... at the final concentration of 5 µM and one of the following anti-human mouse monoclonal antibodies (5 µl per sample) conjugated with allophycocyanin (APC): anti-CD9 (clone MEM-61, Thermo Fisher Scientific), anti-CD63 (clone MEM-259 ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed with PBS and incubated in respective secondary antibodies (donkey anti-mouse AF 488/donkey anti-rabbit AF 568, 1:1000, Thermo Fisher Scientific) and DAPI diluted in blocking buffer (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then washed and probed with horseradish-peroxidase-conjugated donkey anti-mouse or anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (SA1-100, Invitrogen; 31438, Invitrogen) for 1h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... slices were washed with PBS three times for 10 minutes in PBS and incubated with secondary antibodies (respectively: 1:500 goat anti-rabbit IgG, Life Technologies, or 1:500 goat anti-mouse IgG1, Life Technologies) for three hours ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were washed 3x with PBS followed by incubation with Alexa Fluor 488 or 594-conjugated donkey anti-rabbit or anti-mouse secondary antibodies (Invitrogen, Carlsbad, CA) for 1h at RT in the dark ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with the appropriate secondary antibodies (anti-rabbit-A488 Life Technologies, 1:1000; anti-mouse-A568 Life Technologies, 1:1000) for 1 h ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibodies used were goat anti-mouse or goat anti-rabbit polyclonal IgG (H+L) HRP conjugates (ThermoFisher Scientific; #31430 or #32460) (1:5000 - 10,000).
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bioRxiv - Neuroscience 2023Quote: ... then immersed in diluted blocking buffer containing Alexa-Fluor-conjugated secondary antibodies (Goat anti-Rabbit and Goat anti-Mouse IgG1, Invitrogen / Thermo-Fisher), each at 1:800 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were rinsed and incubated in the secondary antibody cocktail (1:200, anti-mouse; Alexa-594, Cat. # A11005 and 1:200, Alexa-488 anti-rabbit, Cat. # A11008; ThermoFisher Scientific, USA) for 4- 5hrs at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The secondary antibodies used were as follows: Alexa Fluor 488 either anti-mouse or anti-rabbit IgG (A11001, A21206, Thermo Fisher Scientific), Alexa Fluor 647 either anti-mouse or anti-rabbit IgG (A21236 ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor 546 goat anti-rabbit IgG and Alexa Fluor 546 goat anti-mouse IgG secondary antibodies were purchased from Invitrogen (Carlsbad, CA).
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bioRxiv - Immunology 2023Quote: ... After three washes of blocking solution, cells were stained with goat anti-mouse (AlexaFluor594, A11005) and anti-rabbit (AlexaFlour488, A11034) antibodies (Invitrogen, 1:1,000) for 1h at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast cells were incubated with 1:1000 dilution of mouse anti-Myc antibody and 1:1000 dilution of chicken anti-His (Invitrogen, PA1-9531) antibodies on ice for 20 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and for 30 min with secondary Alexa488/647-conjugated antibodies (anti-mouse-/anti-rabbit-IgGs: Thermo Fisher Scientific, A-21202/A-31573). Sections were imaged using an Evos FL microscope and marker expression was quantified using CellProfiler 63.
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed and exposed to anti-guinea pig or anti-mouse AF405- or AF488-conjugated secondary antibodies (1:500, Thermo Fisher Scientific) for one hour ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies used for hiPSC-derived neurons and microglia were as follows: anti-rabbit or anti-mouse antibodies conjugated with Alexa Fluor 488 (Invitrogen, 1:1000), anti-rabbit or anti-mouse antibodies conjugated with Alexa Fluor 555 (Invitrogen ...