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Citations for Thermo Fisher :
3501 - 3550 of 10000+ citations for Human Anti NT5E Recombinant Antibody clone Hu101 28 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Goat anti-mouse antibody conjugated with alexa fluorophore 647 and goat anti-rabbit antibody conjugated with alexa fluore 488 (Invitrogen, Inc. CA, USA) were used for immuno-fluorescence (IFA ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunolabeling with performed with mouse anti-B23 primary antibody (1:300; 2h) then detected with Alexa 488 donkey anti-mouse antibody (Invitrogen; 1:500; 1h), then further fixed with 4% paraformaldehyde in DEPC-PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... the secondary antibody (1/400 in blocking buffer, goat anti-rabbit Alexa 594 antibody, Thermo Fisher, A-11037 or donkey anti-mouse Alexa 488 antibody, Thermo Fisher, R37114) was added in blocking buffer for 30 min at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... for use in experiments with anti-HA and anti-catalase antibodies as the primary antibodies and 1:1000 Alexa Fluor™ 488 goat anti-mouse IgG (H+L) (Thermo Fisher Scientific) for use in experiments with the anti-α-tubulin antibody as the primary antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then probed with anti-MyHC antibody (M4276) followed by labeling with secondary anti-mouse antibody Alexa Fluor 488 (A31627, Invitrogen, Carlsbad, CA, USA). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... and the endogenously C-terminal HA-tagged cytochromes were visualized with a Roche Rat anti-HA monoclonal 3F10 primary antibody and FITC-conjugated Donkey anti-Rat 2° antibody (Invitrogen Life Technologies A18746). Images were taken on DIC/brightfield ...
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bioRxiv - Neuroscience 2024Quote: ... incubated for 1.5 hours at room temperature with goat anti-rabbit HRP-conjugated antibody or donkey anti-chick HRP-conjugated antibody (Thermo Fisher Scientific, Massachusetts). Protein expression was detected by chemiluminescence using ECL (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: Microglia activation was also assessed using the rabbit polyclonal Anti-Iba1 antibody (Wako Chemicals; 1:800) and the secondary antibody Donkey Anti-rabbit IgG conjugated with Alexa-594 (Thermo Fisher; 1:500), as previously described ...
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bioRxiv - Microbiology 2023Quote: ... the parasite mitochondrion was visualized using a polyclonal Rabbit anti-HSP60 antibody (Novus NBP2-12734) and AlexaFluor 647-conjugated Goat anti-Rabbit 2° antibody (Invitrogen Life Technologies A21244), the nucleus was stained with ProLong Gold Antifade Mountant with DAPI (Invitrogen Life Technologies P36931) ...
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bioRxiv - Microbiology 2024Quote: ... Alexa Fluor 568 donkey anti-rabbit antibody (Invitrogen, A11011, 1:500, and Alexa Fluor 488 goat anti-rabbit antibody (Invitrogen, A11008, 1:500) in PBS for 1h at RT ...
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bioRxiv - Microbiology 2024Quote: ... cells were probed with Alexa Fluor 568-conjugated goat anti-rat antibody or 488-conjugated goat anti-rabbit antibody (Invitrogen-Molecular Probes, Paisley, U.K.), diluted 1:200 or 1:1000 in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... 1:200 anti-PD-L1 (clone MIH1, eBioscience) and 1:2000 near-infrared (NIR) viability dye (Life Technologies) or isotype controls (FITC ...
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bioRxiv - Bioengineering 2023Quote: ... samples were incubated overnight at 4◦C with either anti-ZO1 (1:100; Clone ZO1-1A12, ThermoFisher Scientific) or anti-CK19 (1:400 ...
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bioRxiv - Biochemistry 2023Quote: ... eFluor™ 450 rat anti-mouse TER-119 clone TER-119 (ThermoFisher, 48-5921-82; 1:100 dilution), APC Mouse Anti-Mouse CD36 clone CRF D-2712 (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: EGR-G01 ES cells were generated in the Ikawa Lab (28) and cultured in KnockOut DMEM (108297-018, Thermo Fisher Scientific) supplemented with 1% Penicillin-Streptomycin-Glutamine ...
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bioRxiv - Microbiology 2021Quote: ... The solution was equally divided between two tubes (72 μL each) and incubated for 30 min at 28 °C with either 8μL of 50mM NMIA (ThermoFisher Scientific, USA) in DMSO (+ reaction ...
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bioRxiv - Synthetic Biology 2021Quote: ... were directly added to yield 50 μL PURE system reaction mixtures [28] containing 13C6 15N4 L-Arginine (R*) and 13C6 L-Lysine (K*) (Thermo Scientific) as a substitute for non-labeled L-Arginine and L-Lysine ...
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bioRxiv - Cell Biology 2021Quote: ... Embryos older than 24 hpf were permeabilized in 10 μg/mL Proteinase K solution (8 min for 24 hpf, 28 min for 48 hpf, 30 min for 5dpf and 7dpf embryos) (Invitrogen, 25530015). ISH was then performed as described previously (Thisse and Thisse ...
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bioRxiv - Developmental Biology 2021Quote: Forelimb buds (E10.0, 28-29 somites) were collected into ice-cold PBS and incubated in cold 2% Trypsin (Gibco 15090-046)/PBS at 4°C for 30min ...
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bioRxiv - Cell Biology 2021Quote: Trypanosoma brucei brucei procyclic SmOxP927 cells (Poon et al., 2012) were cultured at 28°C in SDM-79 medium (Life Technologies) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... albopictus C6/36 (ATCC CRL-1660) and U4.4 were cultured at 28 °C in Leibovitz-L15 medium (Gibco, Carlsbad, CA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... RNA integrity was confirmed by the presence of 18S and 28S bands on a 2% E-Gel EX (Invitrogen cat# G402002). RNA libraries were prepared with NEBNext Poly(A ...
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bioRxiv - Developmental Biology 2022Quote: ... SK- MEL-28 cells were transfected with constructs encoding components of RNP complexes using the Lipofectamine CRISPRmax Cas9 transfection reagent (ThermoFisher #CMAX00015) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated for a further 16 hours at 28°C before collection and storage in Trizol (Thermo Fisher Scientific) ahead of RNA extraction described above ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated for a further 16 hours at 28°C before collection and storage in Trizol (Thermo Fisher Scientific) ahead of RNA extraction ...
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bioRxiv - Immunology 2020Quote: ... and co-cultured in 96 well plates at the ratios indicated in the figure legends in the presence or absence of CD3/28 (Life Technologies) (1:10 bead to cell ...
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bioRxiv - Biochemistry 2023Quote: Carboxysomes were purified as described previously.28 The cells from 1L expression cultures were lysed with 25 mL B-PER bacterial lysis reagent (Thermo Fisher) supplemented with 0.01 mg/mL DnaseI ...
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A modular CRISPR screen identifies individual and combination pathways contributing to HIV-1 latencybioRxiv - Microbiology 2022Quote: The HIV-1 latency cell line J-Lat 5A8 [29] and J-Lat 10.6 [28] were grown in RPMI-1640 medium (Thermo Fisher Scientific) supplemented with 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Neuroscience 2022Quote: ... This assay was conducted in an enclosure that maintains a constant internal temperature (28°C, using Fisher Scientific IsoTemp 6200 R28) and isolates experiments from ambient light and noise ...
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bioRxiv - Neuroscience 2022Quote: ... Plates were incubated at 28°C for three days and isolated colonies present in each plate were counted using a digital colony counter (Fisher Scientific). Five colonies for each morphological type were re-streaked into new plates ...
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bioRxiv - Cancer Biology 2023Quote: ... The siRNA was reverse transiently transfected into SK-MEL-28 and SK-MEL-2 cells using Lipofectamine RNAiMAX® (Life Technologies) following the manufacturer’s instructions and incubated for 72 h/37°C ...
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bioRxiv - Plant Biology 2023Quote: ... CO2 mole fraction inside chamber was controlled by passing all airflow through the CO2 scrubber (indicating soda lime, 4-8 mesh size, CAS: 8006-28-8, Thermo Scientific) while carbon dioxide was supplied by an external cylinder (CAS 124-38-9 ...
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bioRxiv - Immunology 2024Quote: ... Drosophila hemocyte-derived S2 cells were maintained at 28°C in tissue culture flasks containing Schneider’s Drosophila medium (Gibco, Waltham, MA) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... The IFN-β-containing fractions were pooled (∼28 mL) and injected into a 30 mL Slide-A-Lyze cassette with 10K MWCO (ThermoFisher Scientific). The cassette was then dialyzed against 1.8 L of 50 mM sodium phosphate buffer ...
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bioRxiv - Immunology 2024Quote: ... We propagated the virus in C6/36 cells maintained at 28°C on Leibovitz’s L-15 Medium (Gibco, REF:11415-049) with 2% FBS (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... Mice were euthanized 28 days after the injury and identity of traced cells were identified by immunofluorescent staining of podoplanin (PDPN, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells were immobilized in 8-well poly L-lysine-coated IBIDI chambers and stimulated by the addition of CD3/28 Dynabeads (cat. 11452D, Thermo Fisher) for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... as previously published.28 All iPSC lines (control, SLC35A2S304P/Y, SLC35A2-/Y) were maintained under feeder-free conditions on Geltrex-coated plates (Gibco, A1413302) in TeSR-E8 (Stem Cell Technologies ...
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bioRxiv - Immunology 2020Quote: ... and CD4–PE-Cy5.5 (clone S3.5) and IL-17A–PE (clone eBio64DEC17) from Thermo Fisher Scientific.
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bioRxiv - Immunology 2024Quote: ... IgM (clone MADNP5), IgG2b (clone MADNP3) from PARIS-anticorps (Cergy-Pontoise, France) and IgG2c (Invitrogen) were used as standards ...
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bioRxiv - Immunology 2024Quote: ... rat or rabbit IgG (Invitrogen Clone A10043 Lot 753765; Invitrogen Clone A11055 Lot 2211210; Invitrogen A21447 Lot 872645 ...
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bioRxiv - Biochemistry 2024Quote: ... phospho-V2R [pT359/pT360] antibody (1:2000 dilution, 7TM antibodies) followed by HRP-coupled anti-rabbit secondary antibody (1:5000 dilution, Thermo Fisher) for 1 hour each ...
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bioRxiv - Plant Biology 2020Quote: ... Five microliters of the following antibodies were used for immunoprecipitation assays: anti V5 antibody (Invitrogen, #R96025), and purified IgG (A2609 ...
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bioRxiv - Plant Biology 2020Quote: ... The primary antibodies are anti-mCherry monoclonal antibody (16D7) at 1:200 dilution (Thermo Fisher, MA) and anti-α-tubulin antibodies (clone B-5-1-2 ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies used: goat-anti-mouse Alexa Fluor 488 antibody conjugate (1:100, Thermo Fisher Scientific), goat-anti-rabbit Alexa Fluor 555 antibody conjugate (1:50 ...
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bioRxiv - Developmental Biology 2021Quote: ... The following primary antibodies were used: mouse anti-TNNT2 antibody (Invitrogen, Catalog # MA5-12960, 1:50), Chicken anti-GFP antibody (Abcam ...
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bioRxiv - Immunology 2020Quote: ... The following primary antibodies were used for immunofluorescence staining: polyclonal rabbit anti-ZO-1 antibody (Invitrogen, Rockford ...
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bioRxiv - Neuroscience 2020Quote: ... primary antibodies were added (mouse anti-phospho-Tau [Ser202, Thr205] monoclonal antibody [AT8], 1:150, ThermoFisher cat#MN1020 ...
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bioRxiv - Bioengineering 2021Quote: ... The secondary antibody was goat anti-rabbit antibody conjugated with Alexa Fluor 488 (A-11034, Invitrogen™ ...
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bioRxiv - Cancer Biology 2020Quote: ... E-cadherin and β-catenin antibodies followed by anti-rabbit antibody conjugated with Alexa-568 (Invitrogen) and anti-mouse goat antibody conjugated Alexa 488 ...