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Citations for Thermo Fisher :
3501 - 3550 of 10000+ citations for 6 FLUOROCHROMANO 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... the cells were loaded with the calcium indicator Fluo-4 (Fluo-4 NW Calcium Assay Kit, Invitrogen) and 500 nM SiR-tubulin Kit (Cytoskeleton ...
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bioRxiv - Developmental Biology 2024Quote: 4×10^4 hESCs were seeded on 0.5 μg/cm2 Vitronectin-coated 12-well plate (Gibco, A14700) in Nutristem hPSC XF medium (Biological Industries ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were fixed for 30 minutes at 4°C with 4% paraformaldehyde in PBS (Thermo Fisher Scientific), briefly washed three times ...
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bioRxiv - Genomics 2022Quote: Total RNA was extracted separately from testes (n = 4) and ovary (n = 4) tissues using TRIzol (Invitrogen). For each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were separated on a 4-12% NuPAGE 4-12% Bis-Tris protein gels (Thermo Scientific) or 12% Bis-Tris protein gel (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein samples were separated on a 4-12% NuPAGE 4-12% Bis-Tris protein gels (Thermo Scientific) for 1 h at 170 V in 1× NuPAGE MOPS SDS running buffer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were loaded into 4-20% Tris-Glycine gel (Invitrogen Novex WedgeWell 4 to 20%, Tris-Glycine) and run at 150 V for 60 min ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was mixed in a 4:1 ratio with 4-methylvaleric acid (Thermo Fisher catalogue number AAA1540506) in 6% v/v phosphoric acid and copper sulfate pentahydrate ...
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bioRxiv - Neuroscience 2024Quote: ... the heads were dissected and fixed overnight at 4°C using 4% paraformaldehyde (Thermofisher, Cat#: J61899-AP). After several washes the heads were incubated in 20% Sucrose (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Stroma was thoroughly minced and incubated in 4 mg/ml collagenase type 4 (Thermo Fisher Scientific; 17104019) in DMEM with 1 U/ml RNAse inhibitor for 1 h at 37°C in a rotator ...
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bioRxiv - Plant Biology 2020Quote: ... One microgram of DNase-treated RNA was converted to cDNA (SuperScript™ III Reverse Transcriptase, Invitrogen) and used for RT-PCR ...
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bioRxiv - Plant Biology 2020Quote: ... One microgram total RNA was reverse transcribed into cDNA using Maxima Reverse Transcriptase (Thermo Fisher Scientific) as earlier described (Chen et al ...
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bioRxiv - Biophysics 2021Quote: ... One good grid was then imaged in a Titan Krios cryo-electron microscope (Thermo Fisher Scientific) with GIF energy filter (Gatan ...
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bioRxiv - Cell Biology 2020Quote: ... Patient fibroblasts (see above) were transfected with one of these two plasmids using Lipofectamine 3000 (ThermoFisher). Two days later ...
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bioRxiv - Cell Biology 2020Quote: ... RNA quality was determined by examining A260/A280 with NanodropTM One spectrophotometer (Thermo Fisher Scientific Inc). RNA Integrity was confirmed by 1.5% agarose gel electrophoresis ...
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bioRxiv - Microbiology 2019Quote: ... The qRT-PCR reactions were performed in a Step One real time PCR system (Applied Biosystems) using a Micro Amp Fast Optical 48-well reaction plate with 15 μ L reaction volume containing 1 x Power Sybr Green PCR Master Mix (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2021Quote: Single cells or organoids were cultured for one week in DMEM/F12 containing B-27 (Gibco), mouse EGF and mouse FGF basic protein (R&D Systems ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We then checked DNA quality and quantity with a NanoDrop™ One spectrophotometer (Thermo Scientific™).
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bioRxiv - Genomics 2020Quote: ... one set of 14 µl aliquots was resolved on 15% TBE-urea polyacrylamide gel (Life Technologies) at 150 V for 1 hrs and 20 min at room temperature in 1x TBE buffer ...
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bioRxiv - Bioengineering 2022Quote: ... washing solution was carefully removed and one drop of Prolong Gold antifade reagent (Thermo Fisher, #P36930) was placed on top of each organoid ...
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bioRxiv - Cancer Biology 2021Quote: ... with one half fixed and permeabilized with either eBioscience Foxp3/Transcription Factor Staining Buffer Set (Invitrogen), and the other half kept alive in PBS with 2% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... we transformed eight vials of chemically competent One Shot OmniMAX 2 T1R cells (ThermoFisher Scientific, C854003) in parallel each with 200 ng of DNA from the Plasmid Circularization Protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... was used for one-step RT-qPCR and TaqMan Fast Advanced Master Mix (4444556, Life Technologies) was used for non-reverse transcriptase reactions ...
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bioRxiv - Molecular Biology 2020Quote: qPCR reactions were carried out using a Step-One Plus RT-PCR thermal cycler (Applied Biosystems), Luna Universal qPCR Master Mix (New England Biolabs) ...
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bioRxiv - Molecular Biology 2020Quote: ... which was a gift from Robert Campbell (Addgene plasmid #14892).37 Libraries were transformed into One Shot TOP10 Electrocomp E.coli (Thermo Fisher Scientific, Waltham, MA, USA) and plated on LB agar with 0.02 % (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were then split into two fractions and one fraction was treated with TRIzolTM (ThermoFisher) for RNA extraction and the other was resuspended in Laemmli buffer (Bio-Rad laboratories ...
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bioRxiv - Genomics 2020Quote: ... The amplification process was done using SuperScript ™ IV One-Step RT-PCR System kit (Invitrogen) as follows ...
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bioRxiv - Biophysics 2019Quote: ... The plasmids were heat shocked into Escherichia coli One Shot® BL21 STAR™ (DE3) (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... and primer-probe assays (IDT) on a Step One Plus Real-Time PCR System (Applied Biosystems). The AR-V7 primer-probe assay included ...
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bioRxiv - Immunology 2020Quote: ... and amplified with the SuperScript III Platinum one-step quantitative RT-PCR system (Thermo Fisher Scientific). Samples were then run on a LightCycler480 (Roche ...
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bioRxiv - Bioengineering 2019Quote: ... RNA was isolated from one representative biological replicate of transfected cells using Trizol (ThermoFisher, Catalog #15596018) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitive real-time PCR was performed using Step One Plus real-time PCR system (Applied Biosystems) with SYBR premix ...
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bioRxiv - Plant Biology 2019Quote: mRNAs were extracted from the one before last visible leaf samples with Trizol (Invitrogen ref.15596018) and β-mercaptoethanol (SIGMA ref ...
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bioRxiv - Immunology 2019Quote: ... Amplification and analysis was conducted in a Step One Plus real-time PCR machine (Applied Biosystems). Mouse β-actin mRNA was used as an internal control for amplification and relative gene expression levels were calculated using the ΔΔCT method ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were amplified using a SuperScript III Platinum One-Step qRT-PCR kit (Invitrogen 11732-020) with a final reaction volume of 25 µL ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR was performed on a Step-One Plus real-time system (Applied Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Reactions for qRT-PCR were set up using the One-Step qRT-PCR Kit (Thermo Fisher SuperScript III Platinum ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR was performed using SSIII One-Step RT-PCR Kit with Platinum Taq (Invitrogen, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... RT-PCR was performed using SSIII One-Step RT-PCR Kit with Platinum Taq (Invitrogen, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... Five μL RNA was tested with TaqMan™ Fast Virus One-Step Master Mix (Applied Biosystems) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... with one primer containing a biotin residue for linkage to magnetic beads (Invitrogen MyOne Strep Dynabeads). Immobilized templates were incubated with yeast nuclear extracts ...
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bioRxiv - Biophysics 2021Quote: ... One hundred microliters of fixed cells was treated with 0.1 mg/mL RNAse (#EN0531, Thermo Scientific) and incubated in a shaker for 2 h at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Three microliter of ligation reactions were used to transform chemically competent One Shot Top10 cells (Invitrogen) that were then plated on LB agar supplemented with kanamycin (50 μg/ml) ...
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bioRxiv - Molecular Biology 2021Quote: ... One microgram of total RNA was reverse transcribed using SuperScript IV Reverse Transcriptase (Thermo Fisher Scientific) and Oligo dT primers (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... Isolated single neuronal nuclei were directly sorted into CellsDirect One-Step qRT-PCR (Thermo Fisher Scientific) pre-amplification buffers containing 0.14x Taqman gene expression assays and SNP genotyping assays ...
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bioRxiv - Cancer Biology 2020Quote: ... Two sequential sections were placed on one glass slide (SUPERFROST® PLUS, Thermo scientific, Ref J1800AMNZ), and labelled to mark the sectioning order ...
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bioRxiv - Cell Biology 2021Quote: ... RNA concentrations were determined using a NanoDrop 1000 or a NanoDrop One spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription was performed using the SuperScript™ III One-Step RT-PCR System (Invitrogen, 12574026) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... reactions were performed on a Step-one Real-time PCR machine from Applied Biosystems (California, USA) with Fast Universal SYBR Green Master Rox (Roche ...