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Citations for Thermo Fisher :
3451 - 3500 of 7572 citations for Recombinant Human MME Fc Chimera since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Purified recombinant S2P or RBD were biotinylated at a theoretical 1:1 ratio using the Easylink NHS-biotin kit (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3×106 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Genomics 2021Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3E6 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Cell Biology 2021Quote: ... Macrophages were then activated by the addition of 0.2 μg/ml LPS and 0.1 ng/ml murine recombinant interferon-γ (IFNγ) (both ThermoFisher Scientific) for 24 h ...
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp, Thermo Fisher Scientific, USA). RBD-specific nanobodies were mixed with HRP-conjugated recombinant RBD protein (0.2 μg/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... for 3 hr at 37 °C with 10 μg/mL recombinant laminin-521 (BioLamina, LN521-05) diluted in PBS+/+ (Gibco). Single-cell suspensions were then incubated for 3 hr at 37 °C in HUESM medium supplemented with 20 ng/mL bFGF and 10 μM Rock inhibitor Y27632 ...
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bioRxiv - Biochemistry 2020Quote: ... in a donor vector (pDONR-221) and transferred by recombinant cloning into the glutathione S-transferase (GST) fusion vector pDEST15 (Invitrogen). The plasmid was used to transform Escherichia coli ...
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bioRxiv - Immunology 2021Quote: ... LPS-primed THP-1 cell lysates or purified recombinant NLRP3 protein were pre-cleaned using streptavidin magnetic beads (88816, ThermoFisher) to remove nonspecific binding ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then purified by immobilized metal affinity chromatography by using HisPur Ni-NTA (Purify His-tagged recombinant fusion proteins using high-capacity nickel-nitrolotriacetic acid methal-chelate beads) spin columns (ThermoFisher). Recombinant proteins were refolded on the column by gradually removing urea until reaching to a concentration 0 M and then dialyzed against PBS ...
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bioRxiv - Immunology 2022Quote: ... supplemented RPMI (RPMI-1640 supplemented with 10% fetal bovine serum, penicillin-streptomycin, Sodium Pyruvate, MEM NEAA, 30U/ml recombinant IL2 (ThermoFisher) and 55 µM β-mercaptoethanol ...
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bioRxiv - Neuroscience 2022Quote: Recombinant baculovirus was produced using sf9 insect cells following the instructions of Bac-to-Bac TOPO Expression System (Invitrogen A11339). Suspended HEK293S GnTI- cells at the concentration of 3.5-4.0×106/mL in 37°C were infected with P2 virus ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant Breg and GFP protein was labeled with membrane non-permeable biotin according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... Recombinant protein was expressed in 8L culture of Spodoptera frugiperda SF9 cells using the Bac-to-Bac system (Thermo Fisher). Cells were cultured at 27°C ...
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bioRxiv - Plant Biology 2022Quote: ... NiHa-OSR and NoFa-ICL: Fractions containing recombinant protein were pooled and dialyzed using a Slide-A-Lyzer (ThermoFisher Scientific) cassette (20 kDa MWCO ...
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Cbln1 directs axon targeting by corticospinal neurons specifically toward thoraco-lumbar spinal cordbioRxiv - Neuroscience 2022Quote: CSN that project to lumbar spinal segments were retrogradely labeled at P5 with an Alexa Fluor 555-conjugated cholera toxin subunit B (CTB-555) recombinant retrograde tracer (Invitrogen). For these injections ...
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bioRxiv - Zoology 2022Quote: ... Cardiac muscle was detected with a rabbit recombinant polyclonal primary antibody to cardiac troponin I (cTnI, Invitrogen, 1HCLC, 1:600) and a donkey anti-rabbit antibody conjugated to the fluorophore Alexa 647 nm (Invitrogen A31573 ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant plasmid and the empty pBI121 vector underwent digestion using the restriction enzymes XbaI and SacI (Thermo Scientific TM). The purified digested insert and vector ligated using T4 DNA ligase at a 3:1 ratio ...
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bioRxiv - Microbiology 2024Quote: ... One fluorescent variant of aEPEC 1711-4 was obtained by transforming the wild-type strain with the recombinant plasmid pDP151 (Invitrogen), which encodes the fluorescent protein mCherry and confers resistance to ampicillin.
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bioRxiv - Biochemistry 2023Quote: Plasmids for recombinant protein expression were constructed using mega primer insertion PCR [64] using the plasmid Champion pET303/CT-His vector (Invitrogen). The double-stranded DNA inserts (Integrated DNA Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... Cholera Toxin Subunit B (Recombinant) Alexa Fluor™ 488/555/647 Conjugate (CTB-488/555/647, 0.1% wt/vol, 80 nL, ThermoFisher C34775/C34776/C34778 ...
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bioRxiv - Systems Biology 2023Quote: ... assembly was performed by mixing 2-3 sgRNAs (a total of 120 pmol) with 8.5 μg recombinant Cas9 (Invitrogen A36499). The resulting RNP mix was electroporated into 0.3×106 MV411 cells using a Lonza 4D Nucleofector ...
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bioRxiv - Molecular Biology 2023Quote: Introduction of recombinant genes into the parental HEKR4 cell line was performed according to the manufacturer’s guidelines (Thermo Fisher Scientific). In brief ...
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bioRxiv - Neuroscience 2023Quote: ... The recombinant pCS2+ plasmids containing the ORs were purified using the PureLinkTM HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). The plasmids were linearized by NotI (Promega ...
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bioRxiv - Biophysics 2023Quote: ... These cells were then permeabilised using 20 µM digitonin for 5 min and labelled with 8 µg/mL recombinant GST-2xFYVE (69) which was detected using a GST primary antibody (71-7500, Invitrogen) and a Goat anti-Rabbit Alex-aFluor647 secondary antibody (A-21245 ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant GFP and RPH1 proteins were enriched and purified by affinity chromatography using Ni-NTA Superflow resin (Thermo Scientific). Protein concentrations were calculated based on a standard curve created using bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2023Quote: The 6xHis-NBSNIK1 recombinant plasmid has been previously cloned in pDEST17 via LR-mediated recombination (Gateway Invitrogen Life Technologies, Inc). It harbors the NIK1 kinase domain fused to 6xHis under the control of an isopropyl-β-d-thiogalactopyranoside (IPTG ...
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bioRxiv - Cell Biology 2023Quote: Pooled HUVEC sample sets were grown to confluence before treatment with or without recombinant tumour necrosis factor alpha (TNF; 10 ng/mL) (ThermoFisher) in cell culture medium ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant bacmid DNA that carried the mini-HA domain was generated using the Bac-to-Bac system (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... High titre (>3 × 108 viral particles per ml) recombinant baculovirus was obtained using the Bac-to-Bac expression system (Invitrogen) in Sf9 (Spodoptera frugiperda ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant baculovirus generation and large-scale expression in Sf9 cells were carried out using the Bac-to-Bac™ (Invitrogen) expression system.
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bioRxiv - Plant Biology 2023Quote: The 6xHis-NBSNIK1 recombinant plasmid has been previously cloned in pDEST17 via LR-mediated recombination (Gateway Invitrogen Life Technologies, Inc). It harbors the NIK1 kinase domain fused to 6xHis under the control of an isopropyl-β-d-thiogalactopyranoside (IPTG ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific), and recombinant MBP-tagged proteins purification were performed according to the manufacturer’s instructions of Amylose Resin (NEB).
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bioRxiv - Developmental Biology 2023Quote: ... transgenic fragments were further shuffled into the pLenti-Gateway vector via Gateway LR recombinant reaction using Gateway LR Clonase II Enzyme Mix (Invitrogen). In the case of KCNA2 and OVOL1 ...
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bioRxiv - Cell Biology 2023Quote: Generation of replication-defective recombinant adenovirus expressing N-SREBPs under CMV promoter was performed using ViraPower Adenoviral Expression System (#K4930-00, Invitrogen) as described earlier (54) ...
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bioRxiv - Microbiology 2023Quote: Recombinant baculoviruses (AcNPV) were generated using pFastBac1 AcGFP-JEVcore-FOS and a Bac-to-Bac system (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Each tissue was homogenized in 500 μL of lysis buffer containing 400 units of RNase inhibitor (RNaseOUT Recombinant Ribonuclease Inhibitor, 10777-019, Invitrogen) and 0.1% Triton-X100 in low sucrose buffer (0.32 M sucrose ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of the reverse transcription reaction was used to amplify regions of interest using gene-specific primers in a 50 µL PCR reaction using recombinant Taq DNA Polymerase (Invitrogen) (see Table S2) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Recombinant PrP substrates containing the D178N or E200K mutations were created by site-directed mutagenesis with the GeneTailor system (Invitrogen) using the bank vole (BV ...
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bioRxiv - Microbiology 2023Quote: ... and intracellular staining was performed using recombinant hu-mouse anti SeV-NP antibody conjugated with AlexaFluor-647 (1:1000) (Invitrogen) in combination with either anti-Krt5 (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant Rpb4/Rpb7-6His protein (5 μg) was incubated in a 50 μL slurry of HisPur Cobalt Resin (Thermo Scientific) in binding buffer (20 mM Tris [pH 7.5] and 50 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting plasmid was utilized for the generation of recombinant Baculoviruses according to the Bac-to-Bac expression system protocol (Invitrogen). Exponentially growing Sf9 cells (2 x 106 cells/mL in Lonza Insect-XPRESS medium supplemented with 0.1 mM biotin ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... These plasmids were used for the generation of recombinant baculoviruses following Bac-to-Bac expression system protocols (Invitrogen, Catalog # 10359016).
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bioRxiv - Microbiology 2024Quote: ... 1μg recombinant PolyHis-p62 was adsorbed to 5 μl pre-equilibrated packed Ni-NTA resin (Thermo Fisher Scientific, Cat# 88221) via 1 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was produced using random hexamer primers (Mycrosynth) with recombinant M-MuLV reverse transcriptase (RevertAid, Thermo-Fisher Scientific, Cat #K1691) and used for quantitation of TTP-bound RNA and total RNA by RT-qPCR with HOT FIREPol EvaGreen qPCR Supermix (Medibena ...
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bioRxiv - Immunology 2024Quote: Purified recombinant protein vaccines were analyzed on SDS-PAGE by staining with GelCode Blue Safe Protein Stain (Thermo Fisher Scientific). Proteins separated by SDS-PAGE were also transferred onto nitrocellulose membranes for immunoblotting ...
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bioRxiv - Microbiology 2024Quote: ... while the digestion cocktail for RNase HI-positive samples were supplemented with 10 U recombinant Escherichia coli RNase HI (Invitrogen). After digestion ...
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bioRxiv - Immunology 2024Quote: ... or 50 ml of 0.5 mg/ml MSP2 recombinant antigen (McCarthy et al., 2011a) in PBS were coated to 96-well flat bottom MaxiSorb plates (Nunc) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... at 4°C with 2µg anti-CD3 (mouse: eBioscience 14-0031-86; human: eBioscience 16-0037-85) and 1µg anti-CD28 (mouse: Invitrogen 14-0281-86; human: 16-0289-85) and either 7µg of Recombinant PD-L1/B7-H1 Fc Chimera Protein (mouse ...