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Citations for Thermo Fisher :
3451 - 3500 of 10000+ citations for PKC beta 2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Recombinant MARV-Angola GPdTM (IBT Bioservices) was tethered to Fluospheres NutrAvidin-Microspheres yellow-green or red (Thermo Fisher Scientific, Waltham, MA) using the EZ-link Micro Sulfo-NHS-LC-Biotinylation kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... Organoids were cultured in basement membrane extract (BME, Trevingen) and overlaid with growth medium consisting of murine recombinant epidermal growth factor (EGF 50 ng/ml, Life Technologies), murine recombinant Noggin (100 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... 50 mM β- Mercaptoethanol and 1 ng/ml human recombinant leukemia inhibitory factor) for 24 h and then treated with 0.1 μg/ml Colcemid (Gibco, 15210-040) for 2.5 h ...
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bioRxiv - Genetics 2022Quote: ... Secondary antibodies: Goat anti-Mouse IgG (H+L) Superclonal Recombinant Secondary Antibody Alexa Fluor 555 (1:200, ThermoFisher Scientific catalogue #A28180), Goat Anti-Rabbit IgG H&L Alexa Fluor 488 (1:200 ...
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bioRxiv - Microbiology 2022Quote: ... The CHO cell growth and Spike recombinant plasmid transfection were performed according to the ExpiCHO expression system User Guide (MAN0014337, ThermoFisher website). The S-trimer expression was under the control of a strong CMV promoter ...
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bioRxiv - Molecular Biology 2022Quote: ... generally according to the suppliers’ recommendations (1× EPAP buffer, 1 mM ATP, 1 mM DTT, and 0.8 U/μl RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), incubation at 37°C for 1 h) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded in 96-well-plates and treated with 10μg/ml OM-85 concentrate (OM-Pharma) or 100 ng/mL of human recombinant IFN-β protein (ThermoFisher Scientific). The concentration of 10μg/ml of OM-85 was based on previous data with RSV we recently generated [9] ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 700µl FACS buffer with 3.5ul recombinant RNase inhibitor and Hoechst dye (Thermo Fisher, H3570, 1:2000) and incubated 4min on ice covered from light ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ & mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a layer of 0.02 mg ml-1 human recombinant laminin (BioLamina) in DPBS containing Ca2+ and mg2+ (Thermo Fisher Scientific) was deposited on the electrode array ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Genomics 2022Quote: ... 2.5uL of the Slic Reaction is used for subcloning to generate a recombinant donor pFast vector with the Bac-to-Bac™ Baculovirus Expression System (Gibco) as per manufacturer instructions using TOP10 E ...
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bioRxiv - Neuroscience 2024Quote: ... 6-well plates were coated with 20 µg/mL Human recombinant laminin-521 (Biolaminin; Biolamina; LN521-05) in 1x dPBS++ (Gibco; 14040117) and incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... HIV-1 Env and ELC07 Fab used for cryo-EM were produced by transient transfection of Expi293 cells with endotoxin-free preparations of recombinant plasmids using ExpiFectamine 293 (Fisher Scientific). To produce ELC07 Fab ...
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bioRxiv - Developmental Biology 2024Quote: ... 293 cells were transfected with plasmids encoding His-tagged recombinant proteins as described in the Plasmids section using lipofectamine 2000 (Invitrogen, #11668019) following the manufacturer’s instructions (four 100 mm plates each plasmid) ...
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Biochemistry 2024Quote: ... Recombinant neuraminidase and recombinant hemagglutinin were biotinylated in a 20:1 molar ratio of biotin to antigen with EZ-Link NHS-Biotin (ThermoFisher, 20217) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: SK-Mel28 cells were grown on coverslips and transiently transfected with ribonucleprotein complexes consisting of purified recombinant Cas9 protein (Truecut Cas9 Protein v2, Invitrogen, #36499) and synthetic guide RNAs (Invitrogen TrueGuide Synthetic sgRNA #35514 or Negative Control ...
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bioRxiv - Immunology 2024Quote: ... SARS-CoV-2 spike-specific F(ab’)2 was isolated by affinity chromatography using recombinant antigen (1 mg HexaPro) coupled to 0.05 mg dry NHS-activated agarose resin (Thermo Fisher Scientific) as follows ...
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bioRxiv - Bioengineering 2024Quote: ... The cell clumps in Matrigel dome were cultured in the EM supplemented with 25 ng/mL human active BMP7 recombinant protein (Gibco; PHC7204) initially for 3 - 6 days to regenerate and form Exp-HLOs using BMP7 ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5 ng/ml human recombinant epidermal growth factor (EGF) and 50 µg/ml bovine pituitary extract (BPE)(all Thermo Fisher). Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 Spike RBD 319-541 recombinant protein (OBA0101, Ovodan Biotech) was expressed in 25 mL culture using the Expi293F expression system (#A14635; ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... the elutes of GlnR147 (his-TEV GlnR C60SL147C) from Ni-NTA column were cleaved with recombinant tobacco-etch virus protease (Life Technologies) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... and the indicated number of successful recombinant clones was randomly selected and sequenced using a 3500 Genetic Analyzer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... were cultured in complete RPMI (10% FCS, 1% Penicillin/Streptavidin, 1% HEPES) supplemented with recombinant human M-CSF (10 ng/mL, Life Technologies) for 7-10 days to differentiate monocyte-derived macrophages (MDM) ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were treated with the appropriate concentration of recombinant proteins and YO-PRO (Thermo Fisher Scientific, diluted at 1:2000) and incubated at 37°C ...
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bioRxiv - Bioengineering 2023Quote: 20µg of rhEPO and reference product Eprex® were treated with 20 units of recombinant PNGase F (Thermo Fisher Scientific, USA) at 37 ºC for hours ...
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bioRxiv - Cell Biology 2022Quote: ... the His-tagged target protein was eluted with 100% elution buffer at 1 mL min−1 flow rate and the collected fractions checked for recombinant ZNF524 expression on 4-12% NuPAGE Novex Bis-Tris precast gels (Thermo Scientific). His-MBP-ZNF524 of about 75% purity was sent to Pineda Antikörper-Service ...
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bioRxiv - Genetics 2023Quote: ... The presence of recombinant CENH3 proteins on the membrane was always verified by detection with the HisG epitope tag antibody (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: Stem cells were grown on culture plates coated with 5micro μg ml-1 recombinant human Vitronectin (rhVTN-N, Life Technologies, #A14700). HESCs were grown in mTeSR1 (StemCell Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... PIK3CA/PIK3R1 (p110a/p85a) recombinant human protein for use in the experiment shown in Figure 4F was purchased from Thermo Fisher Scientific (catalog number PV4788).
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bioRxiv - Immunology 2023Quote: ... designed using CRISPOR) and 7.5 μg recombinant Cas9 protein (Vienna Biocenter Core Facilities) using the Neon Transfection System (Thermo Fisher Scientific). A week later ...
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bioRxiv - Cancer Biology 2023Quote: ... except for RWPE1 which were maintained in Keratinocyte Serum Free Medium supplemented with bovine pituitary extract and human recombinant epidermal growth factor (manufacturer supplied, Life Technologies). For spheroid cultures ...
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bioRxiv - Cancer Biology 2023Quote: ... LN single cell suspension were stimulated for 5h at 37°C with 5ug/ml of LCMV-GP61-80 in the presence of 50 U/ml recombinant murine IL2 (Thermofisher P2747) and 1mg/ml Brefeldin A (Sigma) ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant containing extracted soluble proteins was used to purify recombinant ChlI by affinity chromatography using a gravity flow column with nickel-nitriloacetic acid resin (Thermo Scientific). The column was equilibrated with 10 column volumes of Tris buffer (see above ...
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bioRxiv - Cancer Biology 2023Quote: H357 and H376 cells at ∼80 % confluence were treated either with distilled water (as control) or with 20 ng/ml of Human Recombinant FGF2 (PHG0026, ThermoFisher Scientific) for 48 h ...
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bioRxiv - Biochemistry 2023Quote: Recombinant trimeric spike proteins (5 µg/mL) were plated in 50 µl in each well on a MaxiSorp (Thermo Fisher Scientific) microtiter plate in 1xPBS and left to incubate for at least 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The concentration of cytokines in the sample was determined by a standard curve generated with recombinant IP-10 (Thermo Fisher Scientific) or rekombinant IFN-α (IFN-a2a ...
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bioRxiv - Genomics 2023Quote: ... HeLa cells were electroporated with the IDT Alt-R CRISPR-Cas9 system (recombinant Cas9, tracrRNA, crRNA) and paired gRNAs using the Neon system (ThermoFisher Scientific). Clonal lines were isolated using the limited dilution method in a 96-well plate format ...
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bioRxiv - Molecular Biology 2023Quote: About 3 ml of bleed 3 was diluted in 7 ml of sterile 1X PBS and passed through 0.2 μM filter and incubated with recombinant protein G sepharose 4B beads (Life Technologies 101242) at 4℃ overnight ...
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bioRxiv - Cell Biology 2023Quote: Saturating amounts of recombinant human (rh) EGFR-Biotin and rhPD-L1-Biotin (ACRO Biosystems) were coated onto separate Streptavidin-coated DynaBeads (Thermo Fisher) using manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... cells were transfected as follows: 400 µg MaxiPrepped recombinant DNA (pcDNA3.1_Strep-Strep-TEV-hPADI6) was diluted in 20 mL Opti-MEM® (Gibco™, #11058021), 1.2 mL of 1 mg/mL PEI 25K™ (Polysciences ...
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Microbiology 2024Quote: ... The endotoxin content of all recombinant antigens was measured using the Limulus amebocyte lysate (LAL)-based Chromogenic Endotoxin Quantitation Kit (Thermo Fisher) following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The mouse was immunized with 50 mg of recombinant hUNG-flRBM-C2 in Imject™ Alum Adjuvant (Thermo Scientific, Catalogue #. 77161). The primary immunization was through IP (Peritoneal injection) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then transfected overnight with 100 pmol of tracrRNA and crRNA complexed with 4 pmol recombinant cas9 protein using RNAiMAX (Thermo Fisher). Medium was then replaced and cells were grown for 48 h before assessing fluorescence and sorting.
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... 2 mM L-glutamine (Gibco) and 100 IU/ml penicillin/streptomycin (Gibco ...
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bioRxiv - Bioengineering 2021Quote: ... N-2 supplement (Gibco, 17502048), B27 supplement minus vitamin A (Gibco ...
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bioRxiv - Bioengineering 2021Quote: ... + 2% human serum albumin (ThermoFisher) + 5 μM ROCKi (Tocris) ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 mM glutamine (Gibco, 25030081), and 1mM sodium pyruvate (Sigma ...