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Citations for Thermo Fisher :
3451 - 3500 of 10000+ citations for Mucin 1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... RPMI-1640 medium supplemented with 10% heat inactivated FCS and final concentrations of 100 U/mL penicillin and 100 μg/mL steptomycin and 2mM L-Glutamine (Gibco). Cells were incubated with OVA peptide (323-339 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured in Dulbecco’s modified eagle medium (DMEM) containing 10% fetal calf serum (FCS) (Thermo Fisher Scientific, Hamburg, Germany), 100 IU/ml penicillin and 100 μg/ml streptomycin (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... and cells were stained with Fixable Viability Dye (eBioscience) and primary antibodies in the presence of Fc block in flow buffer (DPBS [Gibco] supplemented with 2% FCS ...
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bioRxiv - Immunology 2020Quote: ... Biopsies were washed in PBS and transferred into wells containing RPMI-1640 + 10% FCS + 20 μg/mL G418 (Thermo Fisher) + 20 U/mL Pen/Strep and cultured for 24 hours.
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bioRxiv - Molecular Biology 2020Quote: ... the receptor Fc-tagged ectodomains present in the conditioned media were captured on protein A-coated 384-plates (Thermo Scientific), and stored at 4 °C until use ...
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bioRxiv - Cell Biology 2021Quote: ... BSF RNAi cell lines were cultured in HMI-9 medium 47 supplemented with 10% (v/v) low-tetracycline foetal calf serum (FCS; Gibco) at 37 °C in 5% C02 and maintained in 5 µg.mL-1 Hygromycin (InvivoGen ...
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bioRxiv - Cancer Biology 2020Quote: ... The CH12.F3 mouse B lymphocyte cell line (a generous gift from T. Honjo (Kyoto Univ.)) was cultured in RPMI/10% FCS/5% NCTC-109 (Invitrogen)/1X Penn-Strep/50μM αMeOH ...
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bioRxiv - Cancer Biology 2019Quote: ... All media were supplemented with 10% Foetal Calf Serum (FCS) (First Link, Birmingham, UK) as well as 100 units/mL penicillin and 100 μg/mL streptomycin (Gibco™ through Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: Up to 5×106 isolated cells were incubated for 30 min at 4 °C with anti-CD16/CD32 (Fc receptor) clone 93 (Invitrogen) to block non-specific binding and with fixable viability dye eFluor455UV (eBioscience ...
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bioRxiv - Immunology 2022Quote: ... Blots were imaged using the LiCor Odyssey® Fc system after treatment with ECL Western Blotting-Substrat (Thermo Fisher, 32209).
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bioRxiv - Immunology 2022Quote: ... femurs and tibia of mice were flushed with complete medium (RPMI-1640 and 10% heat inactivated fetal calf serum (FCS) (Gibco). Single cell suspensions were incubated with 5 mL of red blood cell (RBC ...
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bioRxiv - Immunology 2022Quote: Surface antibody staining was performed in PBS containing 5% FCS and 1mM EDTA and using a Fixable Aqua Dead Cell (Invitrogen) to determine viability ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: 3T3-F442A preadipocytes were grown and differentiated in a modified DMEM designed for isotope labeling in cell culture [Sigma Aldrich (Saint Louis, MO, USA)] supplemented with 10% dialyzed FCS (GIBCO-Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... HT29 and LIM1215 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% FCS (all from Life Technologies). Cells were maintained in a tissue culture incubator (Sanyo Europe ...
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bioRxiv - Cell Biology 2020Quote: ... Commercial colloidal force probes (CP-qp-CONT-BSA-A, NanoAndMore USA) were passivated with heat-inactivated fetal calf serum (FCS, Invitrogen) for 1h at room temperature to avoid non-specific adhesion of the bead to the cells ...
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bioRxiv - Cell Biology 2019Quote: ... Two x 106 cells/well of a 6-well plate were seeded in DMEM containing 10% FCS (Invitrogen, Carlsbad; USA), 1% HEPES (Carl Roth ...
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped by addition of 0.5 N HCl and absorbance at 450 nm was measured using a Muktiskan FC microplate photometer (Thermo Scientific).
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bioRxiv - Neuroscience 2019Quote: ... with 10% fetal calf serum (FCS) at 37°C in an atmosphere with 5% CO2 and transfected with lipofectamine (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Fc Block was diluted in deficient RPMI (dRPMI; RPMI-1640 supplemented with HEPES and 1g/L sodium azide [ThermoFisher Scientific]) with 10% heat-inactivated FBS (dRPMI+FBS) ...
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bioRxiv - Microbiology 2021Quote: ... The plate was incubated for approximately 5 min before the reaction was stopped with the addition of 2 M H2SO4 and the A450 was measured (Multiskan FC, ThermoFisher). A standard curve was established using serial dilutions of lysate from PA11 ...
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bioRxiv - Immunology 2020Quote: ... washed 2x with PBS 0.5% FCS and then stained with the Click-it Plus OPP Protein Synthesis Kit Alexa Fluor 647 (C10458, Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10 % foetal calf serum (FCS, Labtech) and 100 U/ml penicillin and 100 μg/ml streptomycin (Pen/Strep; Gibco). THP-1 cells were maintained in RPMI (Gibco ...
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bioRxiv - Immunology 2021Quote: ... cells from each vial were thawed in a water bath at 37°C and transferred to 10 mL thawing medium 10% FCS (Gibco) 1% Pen-Strep (Gibco ...
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bioRxiv - Immunology 2021Quote: ... or in sterile Sorting Medium [RPMI 1640 supplemented with 10% (v/v) Heat-Inactivated Fetal Bovine Serum (HI-FCS; A3840001; Gibco)] ...
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bioRxiv - Immunology 2021Quote: ... 1.25 × 105 J76 cells were co-incubated with 2.5 × 105 K562 cells transgenic for HLA-A*02:01 in 96 well plates filled with 200 μL of IMDM media 10% FCS (Gibco) containing serial dilutions peptide of interest in three independent replicates ...
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bioRxiv - Immunology 2022Quote: ... from healthy donors were isolated from whole blood by density centrifugation on Lymphoprep (Axis-Shield PoC) and cryopreserved at −150 °C in FCS (Gibco) + 10% DMSO.
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bioRxiv - Immunology 2022Quote: ... Cells were maintained in complete B cell medium (RPMI supplemented with 10%FCS, 25 mM Hepes, Glutamax, penicilin streptomycin (Invitrogen) and 1‰ß-mercaptoethanol (Sigma Aldrich) ...
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bioRxiv - Bioengineering 2022Quote: ... Stop reaction was added in each well and protein was measured at 450 nm in a Multiskan FC (Thermo Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... UM-SCC-47 cell line was cultured in three parts of Hams F-12 nutrient mixture medium and one part of DMEM with 10% FCS (Gibco) and 100 U/mL penicillin–streptomycin ...
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bioRxiv - Immunology 2022Quote: ... 2020) were cultured in R10 medium (RPMI1640 [Gibco Life Technologies, 31870-025], 10% FCS, 2 mM glutamate [Gibco Life Technologies, 25030-024] ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Content of two ampules containing the cell culture was resuspended in Hank’s solution supplemented with 2% FCS (fetal calf serum) (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... After two washes of the tissue with plating medium (DMEM containing 10% FCS, 2 mM glutamine, 50 mg/ml penicillin/streptomycin, Invitrogen), it was triturated in plating medium and subsequently cells counted ...
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bioRxiv - Immunology 2022Quote: ... the cells were washed thrice with PBS and Minimal Essential Medium α containing additional nucleosides and 10% FCS (Thermo Fisher) was added ...
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bioRxiv - Microbiology 2022Quote: ... and the absorbance at a wavelength of 595 nm was measured using a plate reader (Multiskan FC, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... The optical density was measured at wavelength 405 nm (OD405) by a microplate photometer (Multiskan™ FC, Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: E14tg2a ESCs (RIKEN Cell Bank) were cultured in Glasgow minimum essential medium (GMEM; Wako) containing 10% fetal calf serum (FCS; Invitrogen), 1 mM glutamine (Wako) ...
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bioRxiv - Immunology 2024Quote: ... A single dose of 10 plaque forming unit (PFU) of PVM in 10 µl or the same volume of vehicle (10%, v/v, FCS in DMEM; Gibco) was administered via the intra-nasal (i.n. ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated from whole blood as described for the PBMCs from the patients and cryopreserved at -150°C in fetal calf serum (FCS, Gibco) with 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Immunology 2024Quote: ... Enzymes were quenched with PBS with 2mM EDTA and 2% FCS on ice and then red blood cells were lysed with room-temperature ACK lysis buffer (Gibco) and passed through a 100μm cell strainer.
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bioRxiv - Microbiology 2024Quote: ... a final concentration of 0.1 μg/ml of VHH-Fc in PBS was mixed with 10X SYPRO Orange dye (Life Technologies). Dye binding to molten globule unfolding protein was measured over a 0.01 °C/s temperature gradient from 20 °C to 98 °C in a Roche LightCycler 480 qPCR machine.
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bioRxiv - Genetics 2023Quote: ... resuspended in 800 μL of PBS supplemented with 2 % FCS and analyzed on Attune NxT Flow Cytometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... to block non-specific binding of fluorochrome-conjugated anti-human monoclonal antibodies to Fc receptors before being labeled with a 27-color panel of markers including the viability dye Live/Dead Blue (Invitrogen), following manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: HeLa and HEK293T cells were grown in DMEM (Dulbecco’s modified Eagle’s medium) with GlutaMAX and 10% decomplemented-FCS (fetal calf serum) (Gibco, Life Technologies). HeLa WT and HeLa BST2 KO cells were a kind gift of Stéphane Fremont (Institut Pasteur ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with plasmids expressing bRSV N-GFP and P in DMEM supplemented with 2% FCS.16 h post transfection medium was replaced with Leibovitz’s L-15 medium without phenol red (Gibco, ThermoFisher Scientific), and imaged live with a Leica TCS SP8 confocal microscope using a 63× oil immersion objective ...
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bioRxiv - Immunology 2023Quote: ... were cultured for 24h in 100 μL of RPMI medium containing 10% FCS in the presence of 5 μL of anti-CD3/CD28 beads (ThermoFisher). After centrifugation (450g ...
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bioRxiv - Physiology 2023Quote: ... and grown in DMEM/F12 with 10% FCS and penicillin–streptomycin (Life Technologies, final conc., 50 U/mL of penicillin). Differentiation was achieved by culturing the cells in differentiation media (DMEM/F12 with 2% horse serum and penicillin–streptomycin) ...
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bioRxiv - Cancer Biology 2023Quote: Freshly FACS-sorted cells were plated into 96 wells at 400,000 cells per well for each cell type and cultured for 24-48 hours in 200 ul of RPMI medium with 10% FCS (Gibco), 2 mM glutamine (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: Surface antibody staining was conducted in PBS containing 0.1% FCS and 2mM EDTA for 30 minutes at 4°C to cells that had been pre-incubated with Fc block and LIVE/DEAD Blue (InVitrogen) staining ...
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bioRxiv - Immunology 2023Quote: ... and B16 were cultured inside tissue culture treated flasks with DMEM with 10% FCS (Hyclone) and 1X penicillin-streptomycin (100 units/ml, Gibco) at 37C and 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... All FCS measurements were carried out at ∼20 nM fluorescently labeled protein at 20°C in Nunc chambered coverslips (ThermoFisher). The chambers are pre-incubated with poly-lysine conjugated polyethylene glycol (PLL-PEG ...