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Citations for Thermo Fisher :
3451 - 3500 of 10000+ citations for Fatty acid binding protein FABP1 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... and 0.1 mM Non-Essential Amino Acids (Gibco). iROCK (10 uM ...
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bioRxiv - Microbiology 2024Quote: ... the inoculum was reconstituted in DMEM GlutaMAX (Gibco)/0.1 mM non-essential amino acids (NEAA) (Gibco) at a concentration of 2x 107 CFU/ml ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was purified using Acid-Phenol:Chloroform (Invitrogen) extraction ...
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bioRxiv - Immunology 2024Quote: ... 1x non-essential amino acids (#11140050 Gibco Thermofisher), 10mM HEPES (#15630080 Gibco Thermofisher) ...
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bioRxiv - Microbiology 2024Quote: ... and 1× non-essential amino acids (NEAA; Gibco). All these cells were cultured at 37°C with 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... SYBR Gold Nucleic Acid Gel Stain (S11494, Invitrogen), Thapsigargin (586006 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% MEM Non-Essential Amino Acids (Gibco 11140050), 1% Sodium Pyruvate (Gibco 11360070) ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% MEM non-essential amino acids (Gibco). After 7 days of culture ...
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bioRxiv - Immunology 2024Quote: ... 1x non-essential amino acids (#11140050 Gibco Thermofisher), 10mM HEPES (#15630080 Gibco Thermofisher) ...
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bioRxiv - Neuroscience 2024Quote: ... non- essential amino acids (# 11140050; Thermo Fisher Scientific), monothioglycerol (# M1753 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1x non-essential amino acids (Thermo Fisher Scientific), 50 µM β-Mercaptoethanol (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... and 1X non-essential amino acid mixture (Gibco). Wells in a flat-bottom 96-well plate were loaded with 106 TMCs ...
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bioRxiv - Microbiology 2019Quote: ... lipid membranes were incubated with a 6x-His tag polyclonal antibody HRP conjugate (MA1-21315, ThermoFisher) in blocking buffer for one hour at room temperature followed by three washes with wash buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse SGK1.1 (wild type and constitutively active mutant S515D) cloned into pcDNA3.1/V5-His-TOPO (Invitrogen) were kind gifts from Dr ...
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bioRxiv - Genomics 2019Quote: ... Hi-C DNA bound to capture RNA was isolated using a DynaMag-2 magnet (Life Technologies). Washes (15 min in 500 μl wash buffer I at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Completed reactions were ethanol precipitated and re-suspended in deionized formamide (Hi-Di formamide, Thermo Fisher) with the G-ladders ...
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bioRxiv - Biochemistry 2020Quote: ... optiMEM (1X) reduced serum medium and heat-inactivated fetal bovine serum (HI-FBS) were from Gibco chemicals ...
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bioRxiv - Neuroscience 2021Quote: ... and labeled with 6x-His Tag Antibody coupled to Alexa 555 (Thermo Fisher, MA1-21315-A555). Coverslips were mounted on slides (Aqua-Poly/Mount ...
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bioRxiv - Plant Biology 2021Quote: ... BgtCDA1 coding sequences with a 6XHIS tag were amplified and ligated into pPIC9K-HIS vector (Invitrogen), and then transformated into Pichia pastoris strain GS115 (Invitrogen) ...
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An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: ... All cloning was performed using a pcDNA 3.1-V5/His TOPO-TA cloning kit (Invitrogen K480001) per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... This mix was added to 50 µl pre-washed His-Pur magnetic Nickel beads (Thermofisher Scientific) and incubated for 1 h at 4 °C on a rotor ...
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bioRxiv - Microbiology 2020Quote: ... NR-52285) using the primers in Table II and cloned into pCDNA-V5/His TOPO (Invitrogen). We introduced a FLAG tag to JUNV GP by PCR amplifying it from a previously described expression plasmid (58 ...
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bioRxiv - Developmental Biology 2022Quote: S2 cells were transfected with indicated plasmids or blank pMT-V5-His vector (Invitrogen, Cat# V412020) using the Effectene transfection reagent (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... an 8×His-tag and a Twin-Strep-tag were produced in ExpiCHO-S cells (ThermoFisher) as described previously [10].
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bioRxiv - Cell Biology 2019Quote: ... The his-tag was removed by cleavage overnight with AcTEV™ protease (Invitrogen, Carlsbad, California, USA) at 4ºC according to the provider’s protocol.
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bioRxiv - Biochemistry 2021Quote: 1-2 μg His-tagged calcineurin was first bound to magnetic Dynabeads (Thermo Fisher Sci. USA) in base buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... with 10% FBS (Nucleus Biologics, #AU FBS-500ml L1 HI) and 1% Penicillin-Streptomycin (Gibco, #15140122) and transferred to a new tube ...
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bioRxiv - Neuroscience 2024Quote: ... The buffer was then exchanged to the wash solution (10 % Hi-DiTM Formamide (Thermofisher scientific; 4311320) in 2x saline sodium citrate (SSC) ...
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bioRxiv - Cell Biology 2023Quote: ... The inserts were released by Bam-HI and Eco-RI digestion and ligated to pENTR4 (Invitrogen; previously digested with the same restriction enzymes) ...
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bioRxiv - Plant Biology 2023Quote: ... His-tagged AtKu70/80 was isolated from the supernatant using HisPur Ni-NTA resins (Thermo Fisher) and dialysed in 20 mM HEPES pH7.5 ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid CassetteAv2_pBAD contains the CRISPR03 array cloned into the pBAD/Myc–His B backbone (Life Technologies).12 It was used to prepare CRISPR DNA substrates by PCR with primers MMB1Lead40-5 and MMB1crisp3-r1 using Phusion High-Fidelity DNA polymerase according to the manufacturer’s protocol (New England Biolabs or ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatant fraction was incubated with 2 ml Ni-NTA His-Pur resin (Thermo Fisher, PI88222) for 1 h ...
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bioRxiv - Biophysics 2022Quote: ... The His tagged RBD construct was purified from the culture supernatant of Expi293 cells (Thermo Fisher) that were transfected with the plasmid DNA using FectoPRO (Polyplus Inc.) ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were incubated 10 minutes at 4°C with Dynabeads™ His-Tag Isolation (Invitrogen, 10103D). After one wash with NLB buffer ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... brucei rRAP1-His at 10 µM was incubated with 10 mM SYPRO Orange dye (ThermoFisher Scientific), filtered before use with a 0.22 μm sterile filter (Fisher scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen) has been described previously (13) ...
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bioRxiv - Microbiology 2024Quote: ... The digestion cocktail for RNase HI-negative samples was further supplemented with 120 U RNaseOUT (Invitrogen), while the digestion cocktail for RNase HI-positive samples were supplemented with 10 U recombinant Escherichia coli RNase HI (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... before preparation for sequencing with using Ion PGMTM Hi-QTM View Sequencing Kit (Thermo Fisher Scientific). The library was added to an Ion 318TM Chip Kit v2 BC (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was incubated with an anti-His Rabbit primary antibody (Thermo Scientific, Massachusetts, United States) (1:1000) ...
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bioRxiv - Immunology 2022Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were incubated for 6 hours at 4°C with 5 μg H3K27me3 (07-449 ...
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bioRxiv - Plant Biology 2023Quote: ... Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4 °C for 2 hours with rotation ...
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bioRxiv - Immunology 2022Quote: ... Protein A or protein G Dynabeads (Invitrogen) were prepared according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Protein A and Protein G Dynabeads (Invitrogen) premixed at 1:1 ratio were used for pre-clearing for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2023Quote: Protein-protein crosslinking using BMOE (Thermo Scientific) was carried out according to the product manual ...
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bioRxiv - Immunology 2020Quote: ... Precipitated proteins were bound to 50:50 Protein A: Protein G Dynabeads (Invitrogen) for one to two hours and washed extensively with IP wash buffer (50 mM Tris pH 8 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were incubated with fast-flow protein G- or protein A-Dynabeads (Invitrogen) for 2 hours and centrifuged to eliminate nonspecifically bound proteins ...
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bioRxiv - Cancer Biology 2021Quote: ... using a binary solvent system of 2% acetonitrile with 0.1% formic acid (Solvent A) and 80% acetonitrile with 0.1% formic acid (Solvent B) in an Easy nLC-1000 system (Thermo Fisher Scientific). 2 μL of peptide solution was loaded using Solvent A onto an Acclaim PepMap100 C18 trap column (2 cm x 75 μm inner diameter) ...