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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Rat Carboxypeptidase A3 Mast Cell CPA3 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... The whole-cell protein concentrations are measured by the BCA protein assay (Pierce, Thermo Fisher Scientific). For SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Immunology 2024Quote: ... Proteins were expressed as secreted protein by transient transfection of HEK Expi293 cells (Thermo Fisher Scientific) and purified via CaptureSelect™ C-tag affinity matrix (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: The Subcellular Protein Fractionation Kit for cultured cells (ThermoFisher) was used to isolate the cytoplasm ...
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bioRxiv - Immunology 2021Quote: ... The protein was transiently expressed in Expi293F cells (ThermoFisher). Five to 7 days post-transfection ...
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bioRxiv - Immunology 2021Quote: ... All proteins were transiently expressed in Expi293F cells (ThermoFisher). 5 to 7 days post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were expressed in HEK293 Freestyle cells (Invitrogen) in suspension culture using serum-free media (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... Subcellular Protein Fractionation Kit for Cultured Cells (Fisher Scientific) was used and the protocol was executed through Step 5 to obtain the “membrane extract” ...
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bioRxiv - Microbiology 2020Quote: ... S2Pecto protein was expressed in FreeStyle 293 cells (ThermoFisher) or Expi293 cells (ThermoFisher) ...
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bioRxiv - Molecular Biology 2019Quote: ... All proteins were expressed in Hi-Five cells (Invitrogen) from Trichoplusia ni using Baculovirus and the cultures were harvested 60 hours after infection ...
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bioRxiv - Genetics 2020Quote: ... The protein was expressed in FreeStyle 293F cells (Invitrogen) grown in FreeStyle expression media ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained for surface proteins in PBS (Gibco) or Brilliant Stain Buffer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... proteins were expressed in Expi293F cells (Thermo Fisher Scientific) at 37 °C and 8% CO2 ...
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bioRxiv - Immunology 2022Quote: Viral proteins were produced in HEK293F suspension cells (ThermoFisher) and purified as previously described 8 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Recombinant proteins were produced in Expi293F cells (Gibco, #A14527) following the manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: Total protein was isolated from cells using Trizol (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were extracted using the Cell lysis buffer (Invitrogen). Seven micrograms of proteins were then stacked in the top of a 4-12% NuPAGE gel (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... Proteins were expressed in Expi293F cells (Thermo Fisher Scientific) at 37 °C and 8% CO2 ...
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: Total protein was isolated from cells using Trizol (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... and Mini- Protein Electrophoresis Cells (Invitrogen, Thermo Fisher Scientific). Prestained Protein Ladder (NuPAGE ...
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bioRxiv - Biochemistry 2023Quote: ... and Mini- Protein Electrophoresis Cells (Invitrogen, Thermo Fisher Scientific). Prestained Protein Ladder (NuPAGE ...
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bioRxiv - Microbiology 2023Quote: ... Cell protein lysates were quantified by Bradford assay (ThermoFisher), then separated by Tris-Glycine SDS-PAGE ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were expressed in Expi293F cells (Life technologies). Full-length spike proteins were cloned into the pCAGGS mammalian expression vector as previously described79,80 ...
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bioRxiv - Cell Biology 2019Quote: REF52 cells (an immortal line of post-crisis Fischer rat embryo cells were grown in Minimum Essential Medium α (MEM α) (Gibco/Invitrogen) supplemented with 10% bovine growth medium (BGS ...
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bioRxiv - Cell Biology 2021Quote: ... 105 cells/ml were re-suspended in 500 µl of 2.3 mgs/ml collagen rat tail (Gibco). A 100 µl volume of the collagen and cell mixture was dispensed in quadruplicate wells onto poly-D-Lysine (0.1mg/ml ...
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bioRxiv - Immunology 2021Quote: The INS-1 rat insulinoma cells were cultured at 37°C in RPMI-1640 (Thermal Fisher Scientific) containing 11.2 mM glucose and 2 mM L-glutamine and supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were incubated with CldU secondary antibody (goat anti-rat AF647, ThermoFisher A-21247; Lot# 1696458, 200538) or IdU secondary antibody (goat anti-mouse AF 546 ...
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Development of immortalized rhesus macaque kidney cells supporting infection with a panel of virusesbioRxiv - Microbiology 2022Quote: ... cells were reacted with secondary Alexafluor488-conjugated donkey anti-rat antibody (1:100; Invitrogen, Carlsbad, CA, USA). After three washes in FCS buffer cells were fixated in 2% PFA ...
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bioRxiv - Cell Biology 2022Quote: Cells were seeded into a multi-layer system of collagen (rat-tail collagen used throughout; ThermoFisher # A1048301) and fibrin as shown in Fig ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then stained with PE (Phycoerythrin) mouse/rat anti-CD61 (1:500, Invitrogen; 12-0611-82) and Alexa Fluorβ 647 mouse/human anti-SSEA1 antibodies (1:200 ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then stained with PE (Phycoerythrin) mouse/rat anti-CD61 (1:500, Invitrogen; 12-0611-82) and Alexa Fluor® 647 mouse/human anti-SSEA1 antibodies (1:200 ...
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bioRxiv - Bioengineering 2021Quote: ... were sub-cultured on 0.1 mg/ml rat tail collagen type I (rat tail collagen I, ThermoFisher) T-75 coated flasks and passaged at about 80% confluency ...
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bioRxiv - Neuroscience 2023Quote: ... secondary anti-rat-Alexa Fluor 594 and anti-rat-Alexa Fluor 647 antibodies (1:500, Life Technologies) were used to visualise the HA-tagged channels.
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bioRxiv - Neuroscience 2023Quote: ... primary antibodies were rat α-Kit (1:500, Rat α-Kit clone ACK4, ThermoFisher, product MA5-17836), mouse α-calbindin (1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S; Rabbit, Cell Signaling, cat #7074S; Rat, Cell Signaling, cat #7077S; Sheep, Thermo Fisher, cat #A16041) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein concentrations of cell lysate supernatants were assayed using a BCA protein assay kit (Thermo Scientific). Protein samples were resolved on 8–12% SDS–polyacrylamide gels and transferred to nitrocellulose membranes (Millipore) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were clarified by centrifugation and protein concentration was determined PierceTMBCA protein assay kit (Thermo Scientific). Samples containing equal amount of protein were resolved on 4-12% gradient SDS-PAGE gels and transferred to PVDF membranes ...
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bioRxiv - Biochemistry 2020Quote: The protein concentration of cell lysate was quantified using the Pierce 660 nm Protein Assay (Thermo Fisher).
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bioRxiv - Synthetic Biology 2019Quote: ... and cell lysate protein concentrations were measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). The reaction mixture for CFPS consisted of the following components in a final volume of 8–400 μL containing 1.2 mM ATP ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration of cells was measured using a Micro BCA Protein Assay Kit (Thermo Scientific, Rockford, IL) and used to normalize cytokine levels with final concentrations adjusted to an equivalent of 25 μg of protein for comparison across experiments.
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates containing 500 µg of protein were then incubated with Protein G Dynabeads (Thermo Fisher Scientific) and 2 µg anti-FLAG M2 antibody (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... The protein was transiently expressed as a soluble recombinant protein in mammalian Expi293 cells (Thermo Fisher Scientific). The secreted protein self -assembles into a protein nanoparticle ...
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bioRxiv - Molecular Biology 2023Quote: ... and total cell lysate protein concentrations were measured using the Pierce™ BCA Protein Assay Kit (ThermoFisher).
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bioRxiv - Molecular Biology 2023Quote: ... and total cell lysate protein concentrations were measured using the Pierce™ BCA Protein Assay Kit (ThermoFisher).
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bioRxiv - Microbiology 2022Quote: ... cell lysates were pelleted and protein concentration in supernatant was determined by BCA protein assay (ThermoFisher Scientific). 20 μg total protein from each sample was mixed with 4x Laemmli buffer ...
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bioRxiv - Systems Biology 2023Quote: EV surface protein enrichment was performed by Pierce cell surface protein biotinylation and isolation kit (Thermo Scientific) as previous study37 with a modification on washing procedure (replacing ultracentrifugation by ultrafiltration) ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentrations in cell lysates were quantified using the Micro BCATM protein assay kit (ThermoFisher, Cat# 23235). On a separate nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2023Quote: ... protein lysates were prepared from cultured cells with modified Tissue Protein Extraction Reagent (TPER) (Thermo Fisher Scientific) and a cocktail of protease and phosphatase inhibitors (Roche ...
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bioRxiv - Immunology 2024Quote: ... CyRPA protein were expressed as secreted protein by transient transfection of HEK Expi293 cells (Thermo Fisher Scientific) following the manufacturer’s protocol using ExpiFectamine™ (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... the cell mass was normalized quantifieng total protein using the BCA protein assay kit (ThermoFisher, Cat# 23225).
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bioRxiv - Cell Biology 2019Quote: REF52 cells (an immortal line of post-crisis Fischer rat embryo cells were grown in Minimum Essential Medium α (MEM α) (Gibco/Invitrogen) supplemented with 10% bovine growth medium (BGS ...