Labshake search
Citations for Thermo Fisher :
301 - 350 of 4269 citations for Nipah virus Nucleoprotein N His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: Wild-type and mutant (C139A) USP39 with a Flag-tag at its N-terminal were cloned into the pLenti6/V5 lentiviral vector (Invitrogen, California, USA). Specific shRNA oligonucleotides targeting USP39 were cloned into the pLL3.7 lentiviral vector (Addgene) ...
-
bioRxiv - Biochemistry 2024Quote: ... hamster (Syrian Hamster Isoform X1 – UniProt: A0A1U8BWQ2) and macaque (Macaque – UniProt: F6SVR2) with an N-terminal cMYC-tag were ordered to GeneArt (Thermo Fisher Scientific) and cloned into a phCMV backbone (GeneBank ...
-
bioRxiv - Biochemistry 2024Quote: ... and cloned into a pRK5-derived plasmid with a C- or N-terminal FLAG tag using the gateway vector conversion system (Invitrogen, cat # 11828029). All gene constructs were verified by DNA sequencing.
-
bioRxiv - Microbiology 2019Quote: ... GST-tag and 3xFLAG-tag fusions were achieved using pDEST27 (Invitrogen) and pCI-neo-3xFLAG vector ...
-
bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
-
bioRxiv - Neuroscience 2020Quote: ... which was generating by replacing a V5 tag with a 3HA tag or a 3flag tag from pcDNA3.1-nV5-DEST vector (Invitrogen), and E ...
-
bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
-
bioRxiv - Microbiology 2021Quote: ... 10% HI FBS (Gibco), 1X ultraglutamine (Lonza) ...
-
bioRxiv - Microbiology 2021Quote: ... His (ThermoFisher, MA1-21315), FLAG (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies ...
-
bioRxiv - Microbiology 2021Quote: ... HI FBS (2273356P, Gibco). HEPES (54457 ...
-
bioRxiv - Immunology 2020Quote: ... or anti-His (Invitrogen) for recombinant ZVrecE80 antigen in 0.1M carbonate buffer pH 9.6 ...
-
bioRxiv - Immunology 2020Quote: ... + 10% HI-FCS (Gibco) + 1% Peniciliin/Streptomycin (100x ...
-
bioRxiv - Microbiology 2022Quote: ... anti-penta HIS (Invitrogen) and anti-HSP70 (26 ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10% HI-FBS (Gibco), 1% penicillin/streptomycin (Gibco) ...
-
bioRxiv - Microbiology 2022Quote: ... and expression clones with N-terminal fusion tags were produced simply by Gateway cloning (Gateway™ LR Clonase™ II Enzyme mix, Invitrogen, 11791020). The Lenti-NSP2 was constructed by using the destination vector ...
-
O-GlcNAcylation reduces phase separation and aggregation of the EWS N-terminal low complexity regionbioRxiv - Biochemistry 2021Quote: ... The LCRN coding sequence was inserted in frame with a N-terminal hexahistidine (His6)-SUMO tag in a Champion pET SUMO expression vector (Invitrogen, Carlsbad, CA, USA) by Gibson Assembly (New England Biolabs ...
-
bioRxiv - Biochemistry 2022Quote: ... was cloned into the pFastBac1 vector with a 10xHis tag appended at the N terminus and was expressed in Sf9 cells (Gibco, catalog number 12659017). The cells were cultured at 27 °C for 60 h after being infected with baculoviruses using the Bac-to-Bac system (Life Technologies) ...
-
bioRxiv - Biochemistry 2021Quote: ... NiV RdRp P/L complex was purified using Ni-NTA affinity chromatography based on the P protein N-terminal eight-histidine tag according to the manufacturer’s specifications (Thermo Scientific, Rockford, IL, USA). The protein identities of the purified HCV nsp5b RdRp and NiV RdRp P/L complex were confirmed by mass spectrometry (MS ...
-
bioRxiv - Bioengineering 2021Quote: ... N,N,N’,N’- tetramethyl ethylenediamine and glycylglycine were purchased from Acros Organics (New Jersey, USA). Protogel 30% acrylamide ...
-
bioRxiv - Microbiology 2021Quote: ... 1,2-Bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid tetraacetoxymethyl ester (BAPTA-AM, Invitrogen), calcium Ionophore A23187 (Sigma) ...
-
bioRxiv - Genetics 2020Quote: ... or HA-Tag (Invitrogen) antibody for 1 h at room temperature ...
-
bioRxiv - Systems Biology 2023Quote: ... TMT10plex tags (Thermo Scientific) were dissolved in anhydrous ACN and added to the peptide sample in a 1:10 peptide:TMT ratio ...
-
bioRxiv - Biochemistry 2023Quote: ... anti-SNAP tag (Invitrogen), and DAPI ...
-
bioRxiv - Bioengineering 2021Quote: ... N,N′-Diisopropylcarbodiimide (DIC, Fisher Scientific) and 10 equivalents of DIPEA in DMF for 4 h ...
-
bioRxiv - Biochemistry 2022Quote: ... followed by C-terminal tags (Hisx8-tag, Strep-tag, and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). For SARS-CoV-2 RBD VOCs proteins ...
-
bioRxiv - Immunology 2022Quote: ... followed by a foldon trimerization motif and C-terminal tags (Hisx8-tag, Strep-tag, and AviTag) were synthesized and cloned into pcDNA3.1/Zeo(+) expression vector (Thermo Fisher Scientific). For competition ELISA experiments ...
-
bioRxiv - Neuroscience 2020Quote: ... virus with Trypan Blue (Gibco, 1µl) was injected into the lateral ventricle using glass pipette (WPI) ...
-
bioRxiv - Immunology 2022Quote: ... Virus was diluted in OptiMem (ThermoFisher) and applied apically at a MOI 0.1 for two hours at 34,5°C with 5% CO2.
-
bioRxiv - Bioengineering 2023Quote: ... Varicella zoster virus solution (Fisher Scientific) was dropped onto a No.1 coverslip and dried on top ...
-
bioRxiv - Cell Biology 2023Quote: Cytotune OSKM Sendai virus (A16517, Invitrogen) and EmGFP reporter Sendai Virus (A16519 ...
-
bioRxiv - Immunology 2023Quote: ... which encodes for a C-terminal C-Direct tag containing a free thiol (cysteine) and an EPEA (Glu, Pro, Glu, Ala) purification tag (C-tag, Thermo Fisher Scientific). Vectors were transformed into yeast cells (Saccharomyces cerevisiae strain VWK18 (51)) ...
-
bioRxiv - Bioengineering 2022Quote: ... Final water-PAAm mixtures were crosslinked with 0.5% APS and 0.15% N,N,N′,N′-tetramethylethylenediamine (ThermoFisher, 17919). 50 μL of the mixtures were added to each glass and allowed to crosslink for 30 minutes at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: ... His-tagged CDC50A was detected using a His-probeTM -HRP from Thermo Scientific (15165). Precast stain-free gradient gels for tryptophan fluorescence (4568084 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Vesicular stomatitis virus glycoprotein-pseudotyped virus was produced by co-transfecting 293T cells using Lipofectamine 2000 (Invitrogen) with an shRNA transducing vector and 2 packaging vectors ...
-
bioRxiv - Molecular Biology 2021Quote: ... 10% HI FBS (Gibco A31604), and pen/strep and seeded into 96-well plates 24 hr prior to treatment ...
-
bioRxiv - Neuroscience 2022Quote: ... and pcDNA3.1/myc-His (Invitrogen). Constructs for hTara mutants were prepared by cloning into pEGFP-C3 ...
-
bioRxiv - Bioengineering 2022Quote: ... Primary antibody (Anti-His; Invitrogen, Waltham ...
-
bioRxiv - Immunology 2022Quote: ... + 10% HI-FBS (Gibco, 16140071) + 10% HI-Horse Serum (Gibco ...
-
bioRxiv - Microbiology 2020Quote: ... mouse anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies ...
-
bioRxiv - Molecular Biology 2023Quote: ... His-bind magnetic Dynabeads (Invitrogen) were washed with TBS containing 0.05% Tween-20 (TBS-T ...
-
bioRxiv - Microbiology 2022Quote: ... and 0.5% HI FBS (Gibco).
-
bioRxiv - Microbiology 2022Quote: ... and 0.5% HI FBS (Gibco). Neutrophils were recovered from the bottom of the tube.
-
bioRxiv - Microbiology 2023Quote: ... GFP-His (Thermo Fisher # A42613), NL63 N-His (Sino Biological # 40641-V07E) ...
-
bioRxiv - Cancer Biology 2023Quote: ... with 2% HI FBS (Gibco) and 0.4% 0.5M EDTA (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... anti-His (ThermoFisher scientific, rd230540a), anti-SETD6 (Genetex ...
-
bioRxiv - Microbiology 2024Quote: ... and 10% HI FBS (Gibco). Media were supplemented with interleukin-2 (IL-2 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Hydroxybenzotriazole (HOBt) and O-(benzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HBTU) were purchased from Fisher Scientific. Trifluoroacetic acid (TFA ...