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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Mouse Relaxin 3 RLN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... for 1 h at RT and then incubated for 3 days at 4°C with the monoclonal mouse anti-NeuN antibody conjugated with Alexa-488 (clone A60, Life Technologies) at a concentration of 0.5 µg mL-1 into blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed 3 times in PBS and incubated with the corresponding secondary antibody: goat anti-mouse Alexa-488 (Invitrogen, A32723); donkey anti-mouse Alexa-647 (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2021Quote: ... neurospheres were washed 3 times with PBS and incubated with secondary antibody goat anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific) diluted at 1:1000 for 45 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... We cloned the wild type Cas13a with a 3’ V5 tag (Cas13a-V5) and appended a 3’ UTR from mouse alpha-globin (Genbank accession # NM_001083955) in a pMA7 vector (GeneArt, Thermo Scientific, USA). Additionally ...
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bioRxiv - Cancer Biology 2022Quote: EC stimulation was achieved using 30 ng/ml VEGF-A164 (VEGF-A) (mouse equivalent of VEGF-A165) post 3 hours incubation in serum-free medium (OptiMEM®; Invitrogen). VEGF-A was made in-house as previously described by Krilleke et al [56].
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bioRxiv - Microbiology 2019Quote: ... JE-CVax hyper-immune and non-immune heat inactivated mouse serum samples (starting dilution of 1/9) were serially diluted 3-folds in RPMI 1640 medium (Gibco, ThermoFisher Scientific) supplemented with 4% low IgG serum ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by 3 stringent washes in PBS-tween and application of Alexa flour® 596 conjugated goat anti mouse secondary antibody (A11005, Invitrogen) for 1 hour followed by 3 washes in PBS tween ...
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bioRxiv - Cell Biology 2020Quote: ... A pool of 4 siRNAs targeting mouse SNAP-47 and control Luciferase siRNA (Target Sequence: 5’-CGTACGCGGAATACTTCGA-3’) (Dharmacon, Thermofisher Scientific) were electroporated along with GFP into E15.5 cortical neurons (2 µg GFP + 50 pmol siRNAs) ...
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bioRxiv - Neuroscience 2022Quote: ... primary mouse cortical glial isolated from postnatal day 3 ICR pups were added to the transduced cultures in MEM (Life Technologies) and 10% donor equine serum (HyClone) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times with PBS and AlexaFluor (AF488, AF568) conjugated anti-rabbit or anti-mouse antibodies (#A-11008, A-11004 Fisher Scientific) were added and incubated for 1h at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... The coverslips were washed 3 x in PBS and labeled with donkey anti-mouse AlexaFluor 647 (1:1000, A31571, Thermo Fisher), for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibody incubation was for 3 h with Alexa Fluor 647 goat anti-mouse IgG (1:1000, Thermo Fisher Scientific, A21236) and Alexa Fluor 488 goat anti-rabbit IgG (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... Slides were again washed 3 times with 50 mL of PBS and incubated with anti-mouse IgG-Alexa 488 secondary antibody (1:1000; ThermoFisher A28175) at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were then washed with cold 1X PBS three times for 3 min each and incubated with Alexa 555-labeled anti-rabbit and Alexa 488-labeled anti-mouse secondary antibody (Thermo Fisher) at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 3 times with PBS-T and incubated with 1.6 μg/ml biotin-conjugated goat anti-mouse IgM (Thermo Fisher Scientific) or 4 μg/ml biotin-conjugated goat anti-mouse IgG1 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with secondary antibody (AlexaFluor 546 donkey anti-mouse for PV, goat anti-rabbit for GluR2/3 or NPY, both 1:400, Thermo Fisher). Secondary antibody was dissolved in 1% normal donkey serum for PV or 1% normal goat serum for GluR2/3 or NPY ...
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bioRxiv - Bioengineering 2023Quote: ... and detection antibodies goat anti-mouse-Alexa Fluor 647 (Cat. #A21240) and goat anti-rabbit-Cy3 (Cyanine 3, Cat. #A10520) were obtained from Fisher Scientific.
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bioRxiv - Cell Biology 2024Quote: ... hiPSCs were seeded on CF1 mouse embryonic feeders and maintained for 3 days in ESC medium (KO-DMEM (ThermoFisher, 10829-018), 20 % KO-Serum (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... 10 mins) and resuspended in 3 mL MAGIC buffer supplemented with 60 µL Rabbit anti-Mouse IgM FITC Secondary Antibody (Invitrogen, 31557). The sample was gently rocked at 4 °C for 20 hrs ...
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bioRxiv - Microbiology 2021Quote: ... Neutrophils were labelled with 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530) 15 min prior to recordings ...
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bioRxiv - Microbiology 2021Quote: ... 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit, ThermoFisher Scientific, #53024) and 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G ...
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bioRxiv - Microbiology 2021Quote: ... the murine vasculature was labelled with 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit, ThermoFisher Scientific, #53024) and the human vasculature with 100μg of Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin ...
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bioRxiv - Microbiology 2021Quote: ... and 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530) were injected to label and visualize the human vessels ...
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bioRxiv - Immunology 2021Quote: The total IgG concentration in mouse serum was determined using the IgG (Total) Mouse Uncoated ELISA Kit (Invitrogen, Cat no. 88-50400) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... as previously described [63] and neutrophils were labelled by were labeled with 2.5 µg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530). Mice were infected by intravenous injection of 100 µl of the bacterial inoculum (107 CFU total ...
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bioRxiv - Cell Biology 2020Quote: ... EndoB1 siRNA 5’-UGUUUAUACGACUUGGAGCUU-3’ and 3’-AAGCUCCAAGUCGUAUAAACA-5’ (Invitrogen), control siRNA (Ambion) ...
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bioRxiv - Microbiology 2021Quote: ... FluoZin-3 (FluoZin™-3, AM, cell permeant, Thermo Fisher) was added at 1 mM ...
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bioRxiv - Immunology 2020Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific). The activated beads were washed three times with 50 mM MES pH 5.0 and added to SARS-CoV-2 S protein which was diluted in 50 mM MES pH 5.0 ...
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bioRxiv - Immunology 2022Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific) and incubated for 30 min on a rotator at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... siPALS1 (5’-UUCCUUAUGAUGAACUGGCtt-3’) and siPATJ (5’-CCAGAUACUCACACUUCAGtt-3’, Ambion), siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’ ...
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bioRxiv - Biochemistry 2023Quote: ... DiIC12(3) (1,1’-Didodecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate) (Invitrogen, D383) was used ...
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bioRxiv - Developmental Biology 2023Quote: ... mounting 3 dpf embryos in 3% methylcellulose (Thermo Scientific, 258111000). After imaging ...
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bioRxiv - Developmental Biology 2022Quote: ... 3% FBS (Gibco), 1% MEM non-essential amino acids (Gibco) ...
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bioRxiv - Genomics 2019Quote: ... 3 (Applied Biosystems).
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bioRxiv - Microbiology 2021Quote: ... DiOC2(3) (Invitrogen) was added to a final concentration of 30µM or DiSC3(5 ...
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bioRxiv - Cancer Biology 2022Quote: ... 3% FBS (Invitrogen), 20 ng ml−1 EGF (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... SUMO2/3 (Invitrogen), β-catenin (BD Transduction Laboratories) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 3 (Applied Biosystems). We assembled forward and reverse reads using the Geneious (https://www.geneious.com ...
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bioRxiv - Neuroscience 2023Quote: ... 3% FBS (Gibco), 0.1mM MEM-NEAA (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... Luminex kits that were used are as follows a custom mouse IFN kit (IFN alpha, IFN beta, IL-28, Invitrogen), mouse cytokine 23-plex (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ELISAs for mouse studies were performed for the quantitative measurement of total mouse IgG production in plasma samples using IgG (Total) Mouse Uncoated ELISA Kit (Invitrogen, #88-50400-22) for BALC/c cohorts and IgG (Total ...
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bioRxiv - Physiology 2023Quote: Thirty-six cytokines and chemokines were measured in young and old mouse plasma by luminex multiplex technique using a Cytokine & Chemokine 36-Plex Mouse ProcartaPlex™ Panel 1A kit (Thermo Fisher Scientific). The luminex multiplex assay was performed using undiluted plasma samples following the manufactures instruction.
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bioRxiv - Neuroscience 2019Quote: ... Neurons were then washed 3 times with PBS + TritonX and a secondary anti-mouse antibody Alexa Flour 488 (Life Technologies: 1:1000) was added for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Fifteen μl of a solution at 3 µg/mL of mouse anti-AP (IgGAb-1 clone 8B6.18 Thermo Fisher Scientific; Waltham, MA) in 1X PBS was added to each well of 384-well plates using an automated multichannel pipette (Viaflo Assist ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation of RNAPII was done using 3 μL of Rpb3 antibody (W0012 from Neoclone) coupled to magnetic Pan Mouse Dynabeads (Thermo Fisher Scientific). ChIP and Input samples were labeled with mono-reactive NHS ester fluorescent Cy5 and Cy3 dyes (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunoprecipitation of RNAPII was done using 3 μL of Rpb3 antibody (W0012 from Neoclone) coupled to magnetic Pan Mouse Dynabeads (Thermo Fisher Scientific). ChIP DNA was analyzed by qPCR using primers targeting the 5’ (forward ...
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bioRxiv - Microbiology 2019Quote: ... The slides were then washed with TBST for 3 minutes thrice before adding 1:100 goat anti-mouse IgG-AlexaFluor488 (ThermoFisher, A-11029) or 1:250 goat anti-rabbit IgG-AlexaFluor647 (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... The next day samples were washed 3 times with 1X PBS and incubated with secondary antibody (goat anti- mouse IgG H+L Alexa Fluor 594 (Invitrogen, A-11032)) diluted in blocking buffer for 1 hr at room temperature in the dark ...
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bioRxiv - Microbiology 2019Quote: ... JE-CVax hyper-immune and non-immune heat inactivated mouse serum samples (starting dilution of 1/9) were serially diluted 3-folds in RPMI 1640 medium (Gibco, ThermoFisher Scientific) supplemented with 4% low IgG serum ...
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bioRxiv - Microbiology 2020Quote: ... incubating with primary antibodies (rabbit anti-β-actin, 1:1000, Thermo Fisher Scientific, PA1-183; mouse anti-claudin-2, 3:500, Invitrogen, 325600 ...