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Citations for Thermo Fisher :
301 - 350 of 8540 citations for Lupatadine fumarate EP Impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... followed by 10 cycles of 5’ 60 °C -> 5’ 24 °C (HDV cleavage of construct to release pure R3C) and DNAse I treatment (Thermo Scientific, USA). 75-85 nt randomised oligomer was expressed using artificial DNA template (Eurofins Genomics ...
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bioRxiv - Microbiology 2021Quote: ... followed by 30 minutes with 0.1% Tween/PBS at 37°C and 1 hour at 37°C with secondary antibody Alexa-488 goat anti-mouse (1:500) (Life Technologies #A11029) in 1% BSA/PBS before mounting with Prolong Gold with DAPI (Invitrogen #P36935) ...
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bioRxiv - Microbiology 2020Quote: ... 72°C for 1 min and a final extension at 72°C for 10 min in a thermal cycler (Applied Biosystems Veriti). Deletions in the PCR products were visualized by gel electrophoresis and confirmed by Sanger sequencing ...
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bioRxiv - Biochemistry 2021Quote: ... RBD-C-tag was purified from 116 h time point of the fermentation by C-tag affinity chromatography with the CaptureSelect C-tagXL resin (Thermo Fisher Scientific) according to the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2022Quote: ... 72°C for 5 minutes and then held at 4°C in a Veriti™ 96-well thermal cycler (Applied Biosystems™). Adaptors from the LSK-109 sequencing kit (Nanopore ...
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bioRxiv - Molecular Biology 2022Quote: ... from intron 37 to intron 41 containing the c.5461-10T>C mutation were digested with BoxI and BsiWI (Thermo Fisher Scientific) and ...
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bioRxiv - Developmental Biology 2021Quote: ... and frozen overnight at -80 °C in a NALGENE Cryo 1 °C freezing container (5100-0001, ThermoFisher Scientific, Hanover Park, IL, USA) filled with isopropanol (423830025 ...
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bioRxiv - Molecular Biology 2022Quote: ... 95°C for 20sec then 40 cycles of 95°C for 3sec and 58°C for 45sec on the QuantStudio 5 and 7 qRT-PCR systems (Applied Biosystems, CA). A known negative sample and non-template control (NTC ...
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bioRxiv - Neuroscience 2022Quote: ... 2 minutes at 80°C and then held at 20°C in a Veriti™ 96-well thermal cycler (Applied Biosystems™) until cleavage.
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bioRxiv - Neuroscience 2022Quote: ... supplemented with 5% Fetal Bovine Serum (FBS), 4°C) and incubated (37°C, 2 minutes) in protease solution (TrypLE express, Thermo Fisher 12604013) supplemented with DNase (100 U/ml ...
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bioRxiv - Microbiology 2022Quote: ... Plates were incubated at 4°C overnight and then blocked for 1 hour at 37°C using SuperBlock (ThermoFisher Scientific, Cat. # 37516), and then washed four times with PBS 0.05% Tween-20 (PBST) ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were subjected to a heating ramp from 25°C to 95°C at a rate of 1°C/s using the ABI 7500 Fast Real-Time PCR System (Applied Biosystems, USA). Data was analyzed by Protein Thermal Shift Software v1.4 to obtain melting temperature (Tm).
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bioRxiv - Cell Biology 2023Quote: ... The lysates were digested overnight at 37°C with 1-2 µL of the Lys-C (125-05061, Wako) and trypsin mixtures (Pierce, Thermo Fisher Scientific) (10 ng/μL each) ...
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bioRxiv - Immunology 2023Quote: ... tumor necrosis factor alpha (TNFα) and C-C motif chemokine ligand 2 (CCL2) concentrations were evaluated using the Ready-SET-Go!™ ELISA kits (ThermoFisher Scientific). CCL24 concentrations were evaluated using the Mouse CCL24/Eotaxin-2/MPIF-2 DuoSet ELISA kit (R&D Systems) ...
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bioRxiv - Bioengineering 2024Quote: ... The frozen organoids were sectioned at 8 µm-thick slices on a Leica CM3050S cryostat (-18°C chamber, - 20°C object, 3.5° blade angle) and mounted to silanated microscope slides (Superfrost Plus, Fisher Scientific, Hampton, NH). The slides with sectioned organoids were dried under laminar flow at room temperature and kept at -80°C until staining.
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bioRxiv - Microbiology 2023Quote: ... and 40 cycles of PCR (95°C for 3 seconds, 60°C for 30 seconds) on a QuantStudio 3 (Applied Biosystems, MA).
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bioRxiv - Immunology 2023Quote: ... which encodes for a C-terminal C-Direct tag containing a free thiol (cysteine) and an EPEA (Glu, Pro, Glu, Ala) purification tag (C-tag, Thermo Fisher Scientific). Vectors were transformed into yeast cells (Saccharomyces cerevisiae strain VWK18 (51)) ...
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bioRxiv - Neuroscience 2023Quote: ... We cooled water to 4 °C (Yeosen, PH-F3) and heated water to ∼55 °C using a hot plate (Fisher Scientific, HP88854200) set to 170 °C ...
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Engineering synthetic CopT/A-based genetic circuits for miRNA imaging and functional gene regulationbioRxiv - Synthetic Biology 2023Quote: ... 40 cycles of 94 °C for 30 s and 60 °C for 30 s with ABI 7700 PCR system (Applied Biosystems, USA). U6 snRNA was used as internal control ...
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bioRxiv - Immunology 2023Quote: ... followed by 40 cycles at 95°C for 15 sec then 60°C for 1 min using a QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific). Extracted MAC DNA was used as the quantification standard.
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bioRxiv - Molecular Biology 2023Quote: Visceral fat was flash frozen in liquid nitrogen and then kept at -80°C until thawed at -20°C in RNAlater (ThermoFisher, catalog # AM7030). The tissue was then homogenized in QIAzol Lysis Reagent (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR amplification was performed at 95 °C for 2 minutes and subsequently 40 cycles of 95 °C for 2 s and 60 °C for 20 s on a QuantStudio Real-Time PCR System (Thermo Fisher Scientific). The relative expression of different target RNAs in RNA samples isolated with different methods was analyzed using their Ct values in RT-qPCR ...
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bioRxiv - Cancer Biology 2022Quote: ... The oligos were annealed in a thermocycler with gradual T reduction from 95°C to 25°C at a rate of 5°C/min and subsequently diluted 1:20 into Nuclease free water (ThermoFisher Scientific AM9938). The pLentiCRISPRv2 plasmid was digested for 1 hr at 55°C with 1U per μg of DNA BsmBI-v2 (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... 3D artificial muscles were fixed with buffered 1% paraformaldehyde (PFA) (ThermoScientific, JI9943.K2) overnight at 4°C followed by 6 hours of blocking at 4°C [10% FBS (Gibco, 10270-106), 1% BSA (Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... Extracted protein samples were centrifuged at 20,000 g for 2 min at 4°C and the supernatant was collected for immunoprecipitation using the Pierce™ Anti-c-Myc Magnetic Beads (88843; Thermo-Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... into the reaction mix and incubating it at 37 °C for 15 min and 98 °C for 5 min in the Veriti Thermal Cycler (Applied Biosystems, USA). Quantitative analysis of IL-6 and IFNB expression in THP1 dual reporter cells was performed by StepOne Real-Time PCR Systems (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... were denatured for 10 min at 70°C then reverse transcribed at 37°C for 90 min in a buffer containing Superscript II reverse transcriptase (Thermo Fisher Scientific), DTT (10 mM) ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction mixtures were heated from 10°C to 70°C for approximately 1 hr by a QuantStudio 7 Pro Real-Time PCR System (Thermo Fisher Scientific). Approximately 770 evenly spaced measurements of fluorescence against the temperature gradient were recorded and processed by QuantStudio V1.3 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were sliced into 20-μm sections using a cryostat (temperatures: sample holder −13°C, blade −12°C) (ThermoFisher CryoStar™ NX70) and mounted on superfrost slides (Fisher #1255015) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The reaction was performed at 42 °C for 1 h and 75 °C for 10 min on the 2720 Thermal Cycler (Applied Biosystems, USA).
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bioRxiv - Immunology 2024Quote: ... were added to the eluted phages with a 30-minute incubation at 37°C without shaking and 30 min at 37°C with shaking at 650 rpm in a block heater (Thermo Scientific, USA). Overall ...
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bioRxiv - Plant Biology 2020Quote: ... using LA-Taq (Thermo Scientific, 60°C, 30 cycles). LMJ.RY0402.039504 was subcloned ...
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bioRxiv - Cell Biology 2020Quote: ... Cytochrome c oxidase subunit 1 (COX1; 459600) from Invitrogen; Cytochrome c oxidase subunit 2 (COX2 ...
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bioRxiv - Genetics 2020Quote: ... pAc5.1/V5-His version C (V411020, Thermo Fisher Scientific). In order to test the transferring ability of gypsy ...
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bioRxiv - Cell Biology 2022Quote: ... anti-c-Myc Magnetic Beads (Thermo Fisher Scientific, 88842), donkey anti-mouse IgG secondary antibody (Alexa Fluor 555 ...
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bioRxiv - Genomics 2020Quote: ... 100 µL Pierce c-Myc magnetic beads (Thermo Fisher) were washed twice in ice cold TBST+PI and then added to sonicated lysate and incubated for 16 h at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... Biotin Anti-C-tag Conjugate (Thermo Fisher Scientific, 7103252100); insulin receptor β (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... For qPCR either SYBR GreenER (Invitrogen; Figure S5B-C) or Luna (NEB ...
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bioRxiv - Immunology 2021Quote: ... Samples were stored at −80°C in RNALater (ThermoFisher).
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bioRxiv - Bioengineering 2021Quote: ... two fold dilutions of 3’3’-c-di-AMP (Invitrogen) were made in molecular grade water and added to the protein solution ...
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bioRxiv - Biochemistry 2020Quote: ... Mitomycin C (MMC) was from Thermo Fisher (Waltham, MA). Restriction enzymes from New England Biolabs (Ipswitch ...
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bioRxiv - Systems Biology 2021Quote: ... Frost® Cryo 1°C Freezing Container (ThermoFisher scientific) with Isopropyl alcohol at −80°C over-night ...
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bioRxiv - Microbiology 2020Quote: Tissues were frozen at −80°C in RNALater (Invitrogen). RNA was purified from tissues using QIAzol for lysis and extraction with a Qialyzer ...
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bioRxiv - Bioengineering 2022Quote: ... affinity purified using C-tag affinity resin (Thermo Fisher) using an AKTA chromatography system (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... HRP (ThermoFisher; 1:5000, 60 min at 4°C). Detection was achieved with Pierce™ ECL Western Blotting Substrate using a ChemDoc XR+ (BioRad).
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bioRxiv - Cell Biology 2022Quote: ... dissociated with Accutase (A11105013, Gibco/Invitrogen 5min, 37°C), centrifuged at 1200 rpm for 5min ...
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bioRxiv - Cell Biology 2022Quote: ... 4 °C and normalized by BCA (Thermo Fisher Scientific) using BSA as standard ...
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bioRxiv - Plant Biology 2021Quote: ... anti-c-Myc magnetic beads (Thermo Fisher Scientific, USA) were first washed in RIP dilution buffer (16.7 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... overnight at 4°C and donkey anti-rabbit (ThermoFisher) (1:400 ...
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bioRxiv - Plant Biology 2021Quote: ... We incubated the media at 28 °C (Fisher Scientific 6845 Isotemp Incubator 650D ...