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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Human MXRA8 Mouse Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and HA tag (#26183, Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... V5-tag (Invitrogen 46-0705), STING (CST D2P2F or D1V5L) ...
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bioRxiv - Cell Biology 2023Quote: ... anti-V5 tag (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... V5-tag (46-0705, Invitrogen)(1.12 ug antibody/500 ug protein IP ...
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bioRxiv - Immunology 2024Quote: ... anti-HA Tag (Invitrogen,26183), anti-Flag (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Detection was enabled by addition of 100 µL Goat anti-Human IgG Fc secondary antibody conjugated to horseradish peroxidase (ThermoFisher #A18817), diluted 1:50,000 into PBSM and incubated in the above plate shaker for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Serum samples at varying dilutions were incubated for 2 h followed by detection by a goat anti-human Fc-HRP secondary antibody (ThermoFisher Scientific). The plasma concentration of AvFc was calculated by interpolating from a standard curve ...
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bioRxiv - Genomics 2022Quote: ... Microarrays were then washed (three times with PBS-T for 1 minute) and peptide binding was detected with isotype-specific secondary goat anti-human IgG (Fc) DyLight680 (ThermoFisher Scientific) and goat anti-human IgA (alpha chain ...
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bioRxiv - Cell Biology 2023Quote: ... were cultured in complete RPMI (10% FCS, 1% Penicillin/Streptavidin, 1% HEPES) supplemented with recombinant human M-CSF (10 ng/mL, Life Technologies) for 7-10 days to differentiate monocyte-derived macrophages (MDM) ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed three times with flow staining buffer and labeled with a PE-conjugated anti-Human IgG Fc antibody (Thermo Fisher) for 30 mins ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed 3x in FACS Buffer and stained on ice for 1 hour with goat anti-Human IgG Fc Alexa Fluor™ 647 secondary antibody (ThermoFisher), protected from light ...
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bioRxiv - Cell Biology 2024Quote: ... human TIM4-Fc protein (FUJIFILM Wako Pure Chemical Corporation) was biotinylated with EZ-Link™ Maleimide-PEG11-Biotin (Thermo Fisher Scientific) and conjugated to Dynabeads™ MyOne™ Streptavidin C1 magnetic beads (65001 ...
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bioRxiv - Biophysics 2020Quote: ... while hNAA25 contained a Tobacco-etch virus (TEV)-cleavable N-terminal 6xHis-tag. Human NatB complex (hNAA201-163/hNAA25FL) was obtained by coexpressing these two plasmids in Sf9 (S. frugiperda) cells (ThermoFisher, cat# 12659017), and purified as described previously[35] ...
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bioRxiv - Immunology 2021Quote: ... and RBD-his tag protein-bound antibody was detected with goat-anti-human IgG1 (H+L) with horseradish peroxidase (HRP) conjugated (Invitrogen,1:1000) The plates were washed and developed using TMB substrate solution (Biolegend ...
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bioRxiv - Bioengineering 2021Quote: ... One of the following goat-anti-mouse secondary antibodies was used: IgG Fc-HRP (1:10,000, Invitrogen A16084), IgG1 heavy chain HRP (1:50,000 ...
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bioRxiv - Cell Biology 2020Quote: ... secondary antibody (AlexaFluor488 donkey anti mouse IgM 1/400, Life Technologies in Buffer A, 0.1% Saponin 2% FCS) was performed ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were pre-incubated with mouse-Fc block and viability marker prepared in calcium/magnesium-free PBS (Gibco), before staining with fluorescently-conjugated antibodies prepared in staining buffer (2mM EDTA ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse integrin β3 (armenian hamster monoclonal, 2C9.G3, Thermo Fisher Scientific, 13-0611-81; FC 1:200), anti-mouse integrin α5 (rat monoclonal ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse integrin β1 (Armenian hamster monoclonal, Hmb1-1, Thermo Fisher Scientific, 11-0291-82; FC 1:300), anti-mouse integrin β3 (armenian hamster monoclonal ...
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bioRxiv - Bioengineering 2023Quote: ... One of the following goat-anti-mouse secondary antibodies was used: IgG Fc-HRP (1:10,000, Invitrogen A16084), IgG1 heavy chain HRP (1:10,000 ...
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bioRxiv - Immunology 2021Quote: ... Cell pellets were then incubated with a mouse Anti-V5 tag monoclonal antibody (1.2 mg mL−1; Thermofisher Scientific) in PBS 0.1% BSA for 45 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Protein detection was performed by incubation with a 6x-His Tag mouse monoclonal antibody (clone: HIS.H8; Thermo Fisher Scientific), followed by a secondary goat-anti-mouse HRP antibody (clone NA9310 N ...
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bioRxiv - Developmental Biology 2023Quote: ... Anti-Myc-tag mouse antibody Magnetic beads were added and immunoprecipitated according to the manufacturer’s protocol (MBL and ThermoFisher). Western blotting was performed according to standard techniques.
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bioRxiv - Microbiology 2024Quote: ... Membranes were then incubated for 1 h at room temperature with the following primary antibodies: mouse anti-His-Tag (dilution 1:1,000, clone HIS.H8, Invitrogen MA121315), mouse anti-Flag (dilution 1:1000 ...
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bioRxiv - Immunology 2021Quote: ... and Pacific Blue-conjugated mouse anti-human CD274 (ThermoFisher Scientific, MA, USA) mAbs ...
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bioRxiv - Neuroscience 2020Quote: ... monoclonal mouse anti-human α-synuclein (1:1000, 4B12, Thermo Fisher Scientific); the goat anti-ChAT (1:100 ...
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bioRxiv - Microbiology 2021Quote: ... Mouse anti-human CD4 (clone OKT4; Thermo Fisher Scientific, Waltham, MA, USA) and mouse anti-human BST-2 (clone RS38E ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-human α-synuclein 1:1000 (Thermo Scientific; Rockford, IL, USA); goat anti-luciferase 1:5000 (Abcam ...
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bioRxiv - Pathology 2021Quote: ... and mouse monoclonal anti-human VCAM1 (dilution 1:25, clone 1.4C3, Invitrogen) for 1h at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... PE-labeled mouse anti human CD63 antibody (12-0639-42, Life technologies), Alexa 647 labeled mouse anti human ICAM-1 antibody (sc-107 AF 647 ...
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bioRxiv - Bioengineering 2022Quote: ... Mouse anti-human α-smooth muscle actin (αSMA) monoclonal antibody (ThermoFisher Scientific) was diluted 1:250 in 3% BSA/0.1% tween-20 (Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... monoclonal anti-human lamin B2 (IgG2a, mouse, 1:100) (MA5-17274, Invitrogen), polyclonal anti-human MPO (IgG ...
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bioRxiv - Microbiology 2023Quote: ... received 30 µL of mouse anti-human Ab (Cat# 05-4220, ThermoFisher) at 1 µg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody against human/mouse ABCA1 (#PA1-16789; RRID:AB_2288917) was purchased from Invitrogen Life Technologies (Carlsbad ...
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bioRxiv - Cancer Biology 2023Quote: ... and mouse anti-human CD45RO PE-Cy7 (Invitrogen Cat 25-0457-42). Flow cytometric data were analyzed using SpectroFlo (Cytek ...
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bioRxiv - Microbiology 2023Quote: ... human MARCH4 and mouse MARCH2 (mMARCH2) into pcDNA3.1/myc-His A (Invitrogen) and pBJ5 vector have been previously described (10) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then incubated with FAS Mouse anti-Human (Clone: 4F8H6, Invitrogen) at 1:500 dilution and the appropriate fluorochrome-conjugated secondary antibody (Alexa Fluor 647 anti-mouse IgG1 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Total RNA was extracted from mouse skin tissue or mouse/human EpSCs using Trizol reagent (Invitrogen, Massachusetts, USA), and used as a template for cDNA synthesize using HiScript II reverse transcriptase (Vazyme Biotech ...
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bioRxiv - Biophysics 2023Quote: Full-length human MRS2 (Uniprot Q9HD23) conjugated with a FLAG tag at the C-terminus was expressed in Expi293F cells (Thermo Fisher Scientific, A14527). Cells at a density of 2.0-3.0 × 106 cells/ml were transfected with the MRS2-encoding pCMV plasmid using polyethylenimine (PEI ...
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bioRxiv - Biochemistry 2019Quote: ... at 37°C with 5% CO2 and transfected by different expression constructs for human IgG-Fc with various signal peptide coding regions using LipofectAmine (Invitrogen, Calsbad, CA) as the transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... Recovered nanovials were washed two times with washing buffer and sequentially labeled with streptavidin and biotin goat anti-human IgG Fc (Thermo Fisher, A18821) following standard procedures described above ...
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bioRxiv - Immunology 2024Quote: ... tagged with human IgG Fc was transiently transfected using FreeStyle 293-F cells and purified using Protein G Plus Agarose (Pierce Thermo Fisher Scientific). RBD-SD1 was cleaved from the Fc by incubation with 3C protease (produced in-house ...
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bioRxiv - Microbiology 2021Quote: ... an 8×His-tag and a Twin-Strep-tag were produced in ExpiCHO-S cells (ThermoFisher) as described previously [10].
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bioRxiv - Developmental Biology 2021Quote: ... The following primary antibodies were used: mouse anti-V5-tag to amplify V5-tagged CFP (Thermo Fisher, R96025, 1:650), rat anti-mCherry (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... SAG1 was detected with rabbit anti-SAG1 polyclonal antibody and V5 was detected with mouse anti-V5 tag monoclonal antibody (Invitrogen). Primary antibodies were detected with goat polyclonal Alexa Fluor-conjugated secondary antibodies (Invitrogen) ...
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bioRxiv - Biochemistry 2019Quote: ... Primary antibody immunolabelling was carried out for 1hr at rt with 1:2000 diluted FLAG tag mouse monoclonal antibody (FG4R, ThermoFisher). After incubation with the secondary alkaline phosphatase-conjugated goat Anti-Mouse IgG (H+L ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The presence of AhR and ARNT proteins was verified by Western blot using anti-His-tag (mouse monoclonal, MA1-21315, dilution 1:1000, Invitrogen) anti-FLAG-tag (rabbit monoclonal ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1hr before being incubated overnight at 4°C in and primary antibodies (1:1000 mouse anti-V5 Tag monoclonal Antibody (Invitrogen) and anti-Actin monoclonal antibody (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... the secondary antibodies were chosen against the species of the primary antibody and with different Alexa Fluor color tags (1:500; goat anti-rabbit Alexa Fluor 488, A11004; goat anti-mouse Alexa Fluor 568; A11008, Invitrogen). The rest of the procedures were identical to 4.2.
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bioRxiv - Bioengineering 2024Quote: ... Alexa Fluor 647-conjugated anti-Fc Fab (Surface protein and CYpHER quantitation, ThermoFisher Zenon Labeling Kits, mouse IgG2a for anti-EGFR 199.12 quantitation [ThermoFisher Z25108] ...