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Citations for Thermo Fisher :
301 - 350 of 5122 citations for Human Death Receptors since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Membranes were incubated with the primary antibodies (α-TSPAN5, Aviva System biology #AV46640, 1:500; α-transferrin receptor, Thermo Fisher Clone H68.4 ...
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bioRxiv - Microbiology 2022Quote: ... cells and baby hamster kidney 21 cells expressing the MHV receptor (BHK-R)(47) were maintained in DMEM containing 10% FBS (Invitrogen), 100 U/ml penicillin ...
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bioRxiv - Immunology 2021Quote: ... or with an antibody against the nicotinic acetylcholine receptor ɑ7 (nAChr ɑ7; Alomone Labs) followed by an Alexa594 goat-anti-rabbit conjugate (Invitrogen). Nuclear counterstain with Hoechst 33342 (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... the receptor Fc-tagged ectodomains present in the conditioned media were captured on protein A-coated 384-plates (Thermo Scientific), and stored at 4 °C until use ...
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bioRxiv - Immunology 2020Quote: Up to 5×106 isolated cells were incubated for 30 min at 4 °C with anti-CD16/CD32 (Fc receptor) clone 93 (Invitrogen) to block non-specific binding and with fixable viability dye eFluor455UV (eBioscience ...
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bioRxiv - Biochemistry 2020Quote: ... , S1 subunit mutants and the extracellular domain of ACE2 receptor (GenBank NM_021804.3) were Baculovirus-free produced in High Five cells (Thermo Fisher Scientific) by transient transfection as previously described in Bleckmann et al ...
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bioRxiv - Immunology 2020Quote: ... For neutralization assays HEK 293T expressing the SARS-CoV-2 receptor ACE2 (HEK 293T/ACE2) were cultured in DMEM (Gibco), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: CHO cell lines overexpressing FPR2 and FPR1 receptors were generated in-house and propagated in Ham’s F12 medium (Gibco Biosciences) supplemented with 10% (v/v ...
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bioRxiv - Physiology 2020Quote: ... βTC3 cells were transfected with plasmids encoding mouse furin and/or mouse insulin receptor (pcDNA3.1 backbone) and/or mouse Atp6ap1/Ac45-Flag using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and 20uL was added to each well of a 96 well TaqMan Array plate with chemokine/chemokine receptor primers (ThermoFisher, Mouse Chemokines & Receptors Array plate ...
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bioRxiv - Immunology 2021Quote: ... against Fcγ receptor RI (CD64)-FITC, FcγRIII (CD16)-PE, CD14-PerCP (Becton Dickinson, USA) and FcγRII (CD32)-ACP (Life technologies, USA) and isotype and fluorochrome matched negative control mAbs (BD ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293A cells expressing dopamine receptor sensors or other constructs were prepared on cover-glass-bottom dishes coated with 10 μg/ml of fibronectin (Gibco). Various concentration of dopamine or other ligands were added to the cells ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells expressing either the paternal or maternal allele (or both) of the receptor studied were sorted and expanded for 1 week in RPMI 1640 (ThermoFisher) containing 200 U/mL recombinant IL-2 ...
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bioRxiv - Microbiology 2022Quote: ... E and S1 exposure were conducted in the presence or absence of neutralizing antibodies against toll-like receptor 2 and 4 (TLR2 and TLR4) antibodies (ThermoFisher Scientific Cat ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then processed for real-time polymerase chain reaction (RT-PCR) to assess the expression of the oxytocin receptor (primers and probe assay ID: Rn00564446_g1, purchased from Life Technologies). In particular ...
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bioRxiv - Cell Biology 2022Quote: Commercially available antibodies: mouse monoclonal antibodies to Transferrin Receptor/CD71 (clone H68.4; #13-6890, Thermo Fisher Scientific; western blots (WB) 1:3,000 ...
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bioRxiv - Biochemistry 2023Quote: ... 155-238) were fused to the C-termini of tagged receptors and cloned into the NheI-XhoI sites of pCEP4 (Invitrogen). Plasmids for HIV-1 MN gp160 and BaL gp160 (# 100919 ...
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bioRxiv - Microbiology 2023Quote: ... 293T-hACE-2 (BEI resources, NIH, Catalog No. NR-52511) cells expressing the ACE2 receptors were cultured in DMEM (Gibco) supplemented with 5% FBS (Fetal Bovine Serum) ...
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bioRxiv - Molecular Biology 2023Quote: ... fluorescently labeled GST-antibody (final concentration of 25 nM) and Nuclear Receptor Buffer F (A15668, A11131, PV4547, Thermo Fisher Scientific) supplemented with 1 μM compound or solvent only (DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with anti-CD16/32 (FC receptor block) at 1:100 and LIVE/DEAD Fixable (1:400-1:800; cat no., 15519340; Invitrogen) in 20 μl PBS for 10 min ...
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bioRxiv - Immunology 2023Quote: ... to block non-specific binding of fluorochrome-conjugated anti-human monoclonal antibodies to Fc receptors before being labeled with a 27-color panel of markers including the viability dye Live/Dead Blue (Invitrogen), following manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: HEK-293 cells overexpressing GluA2 or GluA2-TARPγ2 receptors were labeled with 1:4 ratio of maleimide derivatives of Alexa 555 (donor) and Alexa 647 (acceptor) fluorophores (Invitrogen) in extracellular buffer (135 mM NaCl ...
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bioRxiv - Physiology 2023Quote: ... nearly 106 cells were first incubated on ice for 30 minutes in a solution consisting of a 1:10 Fc Receptor Binding Inhibitor (eBiosciences) and 1:500 Live/Dead Aqua stain (Invitrogen) diluted in PBS ...
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bioRxiv - Biophysics 2024Quote: mRNA encoding the ρ1-EM GABAA receptor was produced by in-vitro transcription using the mMessage mMachine T7 Ultra transcription kit (Ambion) according to the manufacturer protocol ...
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bioRxiv - Immunology 2021Quote: ... IL-2 Human Uncoated ELISA kit and IFNγ Human Uncoated ELISA kit (both from Invitrogen) and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... rat anti-human (16G6) and rabbit anti-human polyclonal CD49a were all purchased from ThermoFisher. For collagen measurements 10 mg of allograft tissue/sample was analyzed with a Hydroxyproline Assay Kit (Sigma ...
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bioRxiv - Pathology 2022Quote: ... Aβ42 human ultrasensitive ELISA Kit and Tau (phospho) [pT231] human ELISA Kit from Thermo Fisher, and sAPPα and sAPPβ ELISA Kit from Mybiosource ...
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bioRxiv - Genomics 2021Quote: ... surviving human leucocytes in thawed samples were removed using anti-human CD45 DynaBeads (ThermoFisher Scientific). The resulting parasite pellet was washed to remove soluble human DNA (hDNA) ...
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bioRxiv - Genomics 2022Quote: Human total RNA was extracted from THP-1 human cells using TRIzol (ThermoFisher, cat# 15596026) as per manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... human kinome panel (Cat # 4475388) and human stem cell openarray panel (Cat # 4475390, ThermoFisher, USA). Briefly ...
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bioRxiv - Immunology 2019Quote: All the receptors used in this study were either cloned into bicistronic pEFIN3 vector (Euroscreen) for stable expression or pcDNA3 vector (Life Technologies) for transient expression (S1 Table) ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed for receptor cDNA using validated primers (S4 Table) and SYBR Green Power PCR Mix (Applied Biosystems, ThermoFisher) on an iQ5 real-time qPCR detection system (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed for receptor cDNA using validated primers (S4 Table) and SYBR Green Power PCR Mix (Applied Biosystems, ThermoFisher) on an iQ5 real-time qPCR detection system (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... was added together with labelled α-bungarotoxin (to visualise post-synaptic acetylcholine receptors) in blocking solution for 1.5 h at room temperature (RT) (α-btx, Life Technologies). Then ...
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bioRxiv - Cancer Biology 2020Quote: ... or Insulin Receptor beta (ab69508) overnight at 4°C followed by anti-rabbit secondary antibody conjugated to horseradish peroxidase (ThermoFisher Scientific). Proteins were detected by Luminata Crescendo immunoblot HRP substrate (WBLUR0500Millipore Corporation ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at room temperature with primary antibody against estrogen receptor β (ERβ; 1:500; PA1310B; Thermo Fisher, USA). All antibodies were made up in 1% serum ...
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bioRxiv - Immunology 2020Quote: The Fab fragments for anti-SARS-CoV-2 antibodies ION_300 and ION_360 and SARS-CoV-2 receptor binding domain (RBD) were expressed in Expi293F™ cells (Thermo Fisher, A14527). The antibody:RBD complexes were mixed and co-purified by size exclusion chromatography in 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... For staining FLAG tagged receptors we used anti-FLAG M1 antibody (1:100) labelled with DyLight594 (Thermo Scientific, Cat. no. 46412) dye in presence of 2 mM CaCl2 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The assay procedure is based on the manufacturer’s protocol for LanthaScreen™ TR-FRET Thyroid Receptor beta Coactivator Assay (Invitrogen, PV4686) with slight ...
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bioRxiv - Neuroscience 2020Quote: Cells were incubated with anti-human Fc receptor (0,005 µg/ml eBioscience, 14-9161-73) for 10 min in Medium A without phenol red (HBSS (Gibco, 14170-053) containing 15 mM HEPES (Lonza ...
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bioRxiv - Neuroscience 2020Quote: ... the P2X2 receptor was conditionally expressed into SST+ interneurons and selectively stimulated by laser uncaging of DMNPE-caged ATP (100μM, Life Technologies, UK). LSPS was performed using an ultraviolet (UV ...
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bioRxiv - Microbiology 2020Quote: ... hACE2 receptor (hACE2 Recombinant Rabbit Monoclonal Antibody, 1:50, Thermo-Fisher, Cat#SN0754) and DAPI nuclear stain (1:20,000, Invitrogen, Carlsbad, CA). The primary antibodies were detected with goat anti-rabbit-AP developed with Permanent Red warp (mach3 Biocare Medical).
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bioRxiv - Cancer Biology 2020Quote: ... SSOs specific to insulin receptor pre-mRNA were transfected in cells with either Lipofectamine 2000 (Catalog Number 11668030) from Life Technologies.
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bioRxiv - Plant Biology 2023Quote: ... coding sequences of receptor and effector genes with or without stop codons were either synthesized as pDONR221 entry clones from GeneArt (Thermo Scientific), or were published previously (Saur et al. ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... SNAP-tagged human β2-adrenoceptor or human EP2 receptors fused at the C terminus to a thermostable Nanoluciferase (Hek-ssβ2-AR-tsNluc) using Lipofectamine 3000 reagent (Invitrogen, Paisley, UK). Original SNAP tag and Nanoluciferase sequences were from NEB (Hitchen UK ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then incubated overnight at 4°C with a 1:100 dilution of leptin receptor antibody (cat#PA1-053, ThermoFisher Scientific) in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... A DNA fragment containing the nucleotide sequence of the membrane-bound EGFP link via a T2A cleaving site to the TVATC66 receptor Field 69 coding sequence was designed in an inverted orientation and ordered via GeneArts (ThermoFisher Scientific). This DNA sequence was subsequently amplified by Q5 Hot Start High Fidelity DNA polymerase-based PCR (New England Bioscience ...
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bioRxiv - Cancer Biology 2020Quote: ... using Taqman probes (human RBM10: Assay ID, Hs00275935_m1, human Bcl-xL: Assay ID, Hs00236329_m1, Applied Biosystems, human Bcl-xS ...
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bioRxiv - Biochemistry 2022Quote: ... Human serum (catalog # 4522 from human male AB plasma) and fetal bovine serum were from Gibco, Life Technologies ...