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Citations for Thermo Fisher :
301 - 350 of 5881 citations for Gamma enolase ENO2 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... the media was changed and two days later the cells were collected and plated on gamma irradiated MEF feeders (Gibco, A34181) in human ES media (DMEM/F12 (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... using the Bio-Rad Protein Assay Dye Reagent Concentrate (5000006) with 2 mg/mL Pierce bovine gamma globulin standard ampules from Thermo Fisher Scientific (23212 ...
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bioRxiv - Immunology 2023Quote: ... Eight days after subcutaneous immunisation of C57BL/6 mice with 50 μg of 4-Hydroxy-3-nitrophenyl acetyl hapten conjugated chicken gamma globulin (NP-CGG) in Imject Alum Adjuvant (ThermoFisher Scientific), 4.3 µg MAR hypoxia detecting probe (Goryo Chemical ...
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bioRxiv - Microbiology 2023Quote: ... Cells were always normalized to their optical density at 750 nm using a Helios Gamma UV-Vis Spectrophotometer (Thermo Fisher Scientific). Experiments in day-night conditions were performed in a separate day-night chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... TRAIL blocking ab was purchased from Santa Cruz TRAIL Antibody (RIK-2): sc-56246, and IFN gamma blocking Monoclonal Antibody (NIB42, #6-7318-81) was purchased from Thermo Fisher. The blocking mAb was added when NK cells were combined with tumor cells.
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bioRxiv - Bioengineering 2024Quote: ... under the proliferative condition at 33°C with 10 units/mL mouse interferon gamma supplemented in fresh in RPMI 1640 basal media containing HEPES and GlutaMAX (Gibco, #72400120) with 10% FBS and 1% pen-strep ...
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bioRxiv - Biochemistry 2023Quote: Cells were seeded and allowed to recover for 24 hours prior to being treated with 10 ng/mL interferon gamma (Gibco PHC4031) or vehicle (40 mM Tris pH 7.4) ...
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bioRxiv - Molecular Biology 2021Quote: ... and Western blotting was performed with anti-His (ThermoFisher MA1-135), anti-HA (Roche 11583816001) ...
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bioRxiv - Developmental Biology 2020Quote: ... The Ion Hi-Q Sequencing 200 Kit (Thermo Fisher Scientific, UK) and the Ion Proton platform were used for analysis ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein was purified using His-Pur Ni-NTA Resin (Thermo Scientific), equilibrated using 2 bed volumes of PBS pH=7.4 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... for His-tagged proteins or GST-agarose resin (Thermo Fisher Scientific) for GST-tagged proteins ...
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bioRxiv - Cell Biology 2021Quote: ... pRSET-His-GFP-3C-Survivin (pRSET vector from Thermo Fisher Scientific), pMCNcs-INCENP1-58 and pETM-His-TEV-Borealin10-280 or pETM-His-TEV-Borealin10-221 (pETM vector ...
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bioRxiv - Biophysics 2021Quote: ... Clarified lysate was bound to His-Pur Cobalt resin (Thermo Scientific) for 2 hours with rocking at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The Ion PGMTM Hi-QTM View OT2 kit (Life Technologies, USA) and Ion OneTouch DL instrument (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: ... These fragments were inserted into the pIZ-V5/His vector (Invitrogen) using the In-FusionHD Cloning Kit (Takara) ...
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bioRxiv - Molecular Biology 2022Quote: ... ceylanicum were subcloned into the pMT/BiP/V5-His vector (ThermoFisher) in frame with the BiP signal sequence and the C-terminal V5 and six-histidine tags ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were directly incubated with DyLight680-conjugated anti-His antibody (Thermofisher), washed ...
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bioRxiv - Microbiology 2021Quote: ... 5x-His mouse anti-tag was used at 1:500 (Invitrogen). Anti-mouse HRP secondary antibody was used at 1:10,000 (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The His-MspGH25 cloned into pGAPZαA vector (Invitrogen, Carlsbad, CA, USA) under the control of a constitutive promotor with an α-factor signal peptide for secretion ...
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bioRxiv - Zoology 2019Quote: ... Blots were probed with rabbit anti-His antibody (Thermo Fisher Scientific) at 1 ...
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bioRxiv - Immunology 2020Quote: ... Recombinant His-HMGB1 was produced in 293-freestyle cells (ThermoFisher Scientific). Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant CEACAM-His proteins were expressed in Expi293F cells (Life Technologies) and purified by affinity chromatography (ÄKTA Pure ...
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bioRxiv - Microbiology 2021Quote: ... using an Ion PGM Hi-Q Sequencing Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions to ensure a high degree of sensitivity required and the deal with the high complexity of our samples under investigation.
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bioRxiv - Biophysics 2019Quote: ... 1 nM LanthaScreen Elite Tb-anti-HIS Antibody (Thermo Fisher Scientific), and 5 nM Fluormone Pan-PPAR Green (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... and His-BMAL1 FL were co-infected into monolayer HighFive (Invitrogen) insect cells for protein expression ...
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bioRxiv - Evolutionary Biology 2021Quote: ... suspended in Hi-Di formamide (Applied Biosystems, Foster City, CA, USA) and run on an ABI 3730 at the University of Georgia ...
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bioRxiv - Immunology 2020Quote: ... and 10% heat-inactivated fetal bovine serum (HI-FBS, Invitrogen, 12484028). DMEM supplemented media consisted of 2 mM GlutaMAX ...
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bioRxiv - Immunology 2021Quote: ... washed with 1% HI-FBS RPMI-1640 (ThermoFisher Scientific cat. 11875085) and red blood cells were lysed using 1x RBC Lysis Buffer (eBioscience ...
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bioRxiv - Immunology 2021Quote: ... 1◻nM LanthaScreen Elite Tb-anti-His Antibody (ThermoFisher #PV5895), and 400 nM FITC-labeled PGC1α peptide (residues 137–155 ...
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bioRxiv - Molecular Biology 2020Quote: ... The empty coding vector pcDNA3.1/MYC-His was obtained from Invitrogen and pCMV-β-Galactosidase was obtained from Clontech/Takara Bio.
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bioRxiv - Molecular Biology 2022Quote: ... the following antibodies were used: 6x-His Tag monoclonal antibody (ThermoFisher, cat ...
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bioRxiv - Microbiology 2023Quote: ... 1-2 μl of primary anti-His (PA1-983B, Thermo Scientific) or anti-alpha RNAP (4RA2 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µL of His-Tag magnetic beads (Dynabeads™ Invitrogen #10103D) was added to each tube ...
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bioRxiv - Microbiology 2023Quote: ... and inserted into similarly digested pcDNA4-To/myc-His B (Invitrogen), resulting in pcDNA4-L3HYPDH ...
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bioRxiv - Microbiology 2023Quote: ... 10% heat-inactivated fetal bovine serum (HI-FBS; Hyclone and Gibco), and 100 U/mL penicillin/streptomycin (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... His-HcpGA1 was purified using the ProBond Purification System (Life Technologies) and the eluted protein was dialyzed against PBS and used as immunogen for antiserum generation.
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% heat-inactivated fetal calf serum (HI-FCS, Gibco) and 1% of Penstrep (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were taken for immunoblotting using anti His-tag antibody (ThermoFisher). Crosslinking and control reactions intended for scramblase activity assays were stopped with 1 M Tris pH 7 to a final concentration of 10 mM ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% (v/v) heat-inactivated FBS (HI-FBS; Gibco). Immortalised adult human C20 microglial cells were kindly provided by Dr David Alvarez Carbonell ...
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bioRxiv - Biochemistry 2023Quote: ... All media was supplemented with 10% HI FBS (Gibco cat # 10438026).
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bioRxiv - Biochemistry 2023Quote: ... Hi Five cells grown in Sf-900 II SFM media (Gibco) at a density of 2.0 × 106 cells/mL were infected with baculovirus at 1.5% v/v ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with Novex Hi-Density TBE Sample buffer (ThermoFisher Scientific, LC6678) and analysed on 10% Novex TBE gels (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 10% fetal bovine serum (Gibco FBS HI, Cat# A5256701) and 1% penicillin-streptomycin (Thermofisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... followed by Western blot analysis using anti-His antibody (Invitrogen, USA).
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bioRxiv - Microbiology 2023Quote: ... 2 µL of Novex Hi-Density TBE 5X Sample Buffer (Invitrogen) was mixed with 8 µL of the completed EMSA reaction and loaded on an 8% acrylamide gel ...
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bioRxiv - Biophysics 2023Quote: ... To cut the His-tag HRV 3C protease (Thermo-Fisher Scientific) was added to a molar ratio of 1:800 (enzyme ...
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bioRxiv - Synthetic Biology 2023Quote: The determination of serum TNF-α and IFN-γ concentration was performed using Mouse IFN gamma Uncoated ELISA and Mouse TNF alpha Uncoated ELISA (Invitrogen). The protocols recommended by the vendor were strictly adhered to.
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bioRxiv - Biophysics 2023Quote: ... Absorbance values over 600-900nm were measured using a Thermo Spectronic Helios Gamma UV-Vis Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). This was compared to analytical data from Aymard et al ...
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bioRxiv - Plant Biology 2019Quote: ... The mixture was analyzed by immunoblotting anti-6×His (Invitrogen, #MA1-135), anti-MBP (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2022Quote: ... His-tagged proteins were purified using HisPur Ni-NTA Resin (Thermo Fisher). Resin-bound proteins were washed (25 mM Imidazole ...