Labshake search
Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Corticosterone 17 Deoxycortisol CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: HEK293T/17 cells were cultured at 37°C and 5% CO2 in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, 10567-014) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Developmental Biology 2019Quote: Lysates of stage 17 embryo expressing a tracheae-specific Yki-V5 construct were immunoprecipitated (described above) using the mouse anti-V5 antibody (Invitrogen). After electrophoretic separation on SDS gel and Coomassie staining ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... and 0.5 µM of Oligo 17 (Supplementary File 2) as forward and reverse primers in 1x PCR buffer (Thermo Scientific), 0.2 mM dNTPs ...
-
bioRxiv - Cell Biology 2020Quote: HeLa and 293T/17 cells (UCSF tissue culture facility) were cultured in Advanced Dulbecco’s Modified Eagle’s medium (DMEM, Thermo Fisher Scientific) supplemented with 2% fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2022Quote: ... Liquid cultures of Mtb were grown at 37 °C with shaking in Middlebrook 7H9 (Thermo Fisher Scientific, DF0713-17-9) broth supplemented with 0.02 % tyloxapol and albumin (Millipore Sigma ...
-
bioRxiv - Immunology 2022Quote: ... stimulation for analysis of cytokine production using a Luminex-based multiplexed assay (Th1/2/9/17 18-plex Human ProcartaPlex Panel, ThermoFisher) per manufacturer recommendation ...
-
bioRxiv - Biochemistry 2023Quote: ... Protein material was separated via SDS– polyacrylamide gel electrophoresis (SDS-PAGE) (12, 15, or 17 well gels, 4-20% Bis-tris BOLT gels, Invitrogen), with 1X MOPS Buffer (Invitrogen ...
-
bioRxiv - Cancer Biology 2023Quote: ... APC or APC-eFluor780-conjugated CD45 (Thermo Fisher Scientific Cat# 17-0451-83, Thermo Fisher Scientific Cat# 47-0451-82); BUV563-conjugated CD49b (BD Biosciences Cat# 741280) ...
-
bioRxiv - Molecular Biology 2023Quote: ... immediately followed by a triple MS2 stem-linker sequence (PNAS September 17, 2002 99 (19) 12203-12207) was purchased from ThermoFisher. This was used as a template to make pcDNA3.0_NORAD_NRU78_MS2 ...
-
bioRxiv - Biochemistry 2023Quote: Agar plate preparation: 7.5g agar (BD Difco™ Dehydrated Culture Media: Potato Dextrose Agar, Fisher Scientific, Cat# DF0013-17-6) was added to the 500 mL of liquid media ...
-
bioRxiv - Bioengineering 2022Quote: ... and 200ng of pMD2.G) were transfected into 2 x 105 HEK293T/17 cells using Lipofectamine 2000 (Thermo Fisher Scientific) by the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... and HEK293T/17 cells (ATCC CRL-11268) were maintained in DMEM (L-glutamine, Sodium Pyruvate, Non-essential amino acids; Gibco) supplemented with 10% FBS (Gibco ...
-
bioRxiv - Bioengineering 2023Quote: Plasmids were transfected as a stoichiometric mixture (21 μg) in HEK293T/17 cells using Lipofectamine 2000 and Plus Reagent (Invitrogen) together with psPAX2 (15 μg ...
-
bioRxiv - Molecular Biology 2023Quote: ... as already described [17] and reseeded onto 0.2 mm bone slices labelled with N-hydroxysuccinimide ester-activated rhodamine fluorescent dye (ThermoFisher Scientific), at a cell density of 1x105 cells/bone slice (Boneslices.com) ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were stained for 30 min on ice with pre-optimized concentrations of the following fluorochrome-conjugated antibodies: AREG-APC (17-5370-42; Invitrogen), CD49f (ITGA6)-PE (561894/555736 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Lentiviral particles were produced by co-transfection of the sgRNA library with lentiviral packaging plasmids pMD2G and psPAX2 in HEK293T/17 cells using Lipofectamine 3000 (Invitrogen). pMD2.G and psPAX2 were gifts from Didier Trono (Addgene plasmid #12259 and #12260) ...
-
bioRxiv - Neuroscience 2024Quote: ... samples were diluted to 1 M urea with a mixture of 360 μL of 50 mM ammonium bicarbonate (17) and 5 μg of Trypsin (ThermoFisher). The samples were subsequently incubated overnight at 25 °C for digestion with trypsin ...
-
bioRxiv - Biophysics 2024Quote: ... On-grid lamellae were prepared at a stage tilt of 17° with a 45° pre-tilted shuttle (Thermo Fisher Scientific) resulting in a +-10° pre-tilt in the TEM similar to published protocols [22 ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were suspended in MACS buffer (Miltenyi) and incubated with 10μl/ml anti-EpCAM-APC (Thermo Scientific, 17-5791-83) and/or anti-NGFR-PE (eBioscience ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 mL of cells (OD600 < 1.0) was spun down at 10k g-force for 1 min (Thermo Fisher Scientific, Pico 17), resuspended in 200 μL SCGE and sonicated for 5 s (Bandelin electronics ...
-
bioRxiv - Molecular Biology 2019Quote: HEK-293T cells (293T/17, ATCC) were maintained in high-glucose DMEM supplemented with 10% fetal bovine serum (FBS, Life Technologies). U2OS cells (ATCC ...
-
bioRxiv - Cancer Biology 2021Quote: ... into single-cell suspension and labeled using double staining with anti-TRA-1-81 APC (#17-8883-42, Thermo Fisher Scientific) and SSEA4 Alexa Fluor 488 (#14-8843-80 ...
-
bioRxiv - Cell Biology 2021Quote: ... and pCMV-dR8.91 (Zufferey et al., 1997) (5 mg each) were transfected into HEK 293T/17 cells using Lipofectamine LTX (Thermo Fisher Scientific). Media containing lentivirus was collected 48 h and 72 h following transfection and filtered using a 13 mm syringe filter with a 0.45 µm PVDF membrane (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were seeded on coverslips at densities of 1.2–4 × 104 cells and transfected with siRNAs at final concentrations of 10–17 nM using RNAiMax (Life Technologies Corporation) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were seeded on coverslips at densities of 1.2–4 × 104 cells and transfected with siRNAs at final concentrations of 10–17 nM using RNAiMax (Life Technologies Corporation) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... using BamHI/EcoRV restriction sites included in the oligonucleotides (underlined) and then transferred to the pTcINDEX-GW and to pDEST™17 (Gateway System, Invitrogen) vector by recombination using LR clonase II enzyme mix (Invitrogen).
-
bioRxiv - Neuroscience 2022Quote: ... The footprint samples were size-selected using the 17 and 34 nt markers as a guide on a 15% TBE-Urea polyacrylamide gel (Thermo Scientific), while total RNA samples were randomly heat fragmented by incubating at 95 °C in an alkaline fragmentation solution (50 mM NaCO3 pH 9.2 ...
-
bioRxiv - Cell Biology 2022Quote: ... HEK293T/17 cells (ATCC) were seeded at a density of 6×104/cm2 in Eagle’s Minimum Essential Medium (EMEM, Thermofisher, Waltham, MA), containing 10% FBS ...
-
bioRxiv - Genomics 2022Quote: ... and a 1:400 dilution of Anti-Mouse Thy-1.2 antibody conjugated to APC (Thermo Fisher Scientific, Cat#: 17-0902-81).
-
bioRxiv - Neuroscience 2019Quote: ... constructs were injected into the syncytial gonad of young adult worms at concentrations ranging from 5 to 50 ng/µL with 50 ng/µL of the coinjection marker elt-2p::GFP or unc-122p::dsRED and 17 ng/µL of a 1-kb DNA ladder (Thermo Scientific) as carrier DNA.
-
bioRxiv - Molecular Biology 2019Quote: ... U2OS cells were resuspended in complete media and 1×105 cells were mixed with pAAVS1-PDi-CRISPRn knockin vector (0.25 ug) and each AAVS1 TALEN pair (0.1 ug) and nucleofected using the Neon® Transfection System (program 17; Life Technologies). Polyclonal population was selected with 2 μg/ml of puromycin (Bioshop cat# PUR555) ...
-
bioRxiv - Molecular Biology 2020Quote: ... ref.17-1318-02) before being subjected to SDS-PAGE gel electrophoresis (NuPage™ 4-12% Bis-Tris Gel Invitrogen, NP0322box) and semi-dry transfer to a nitrocellulose membrane (iBlot®2NC Mini Stacks Invitrogen ...
-
bioRxiv - Systems Biology 2021Quote: ... The final transformation mixture contained 10 mM LiAc (Lithium acetate dihydrate, 98%, extra pure, ACROS Organics™, CAS 6108-17-4), 50 µg ssDNA (UltraPure™ Salmon Sperm DNA Solution ...
-
bioRxiv - Genetics 2020Quote: ... cells were processed for surface marker / enucleation analysis by staining with anti-CD71 (PE-Cy7 conjugated, eBioscience #25-0719-42), anti-CD235a (APC conjugated, eBioscience #17-9987-42) and Hoechst 33342 (Invitrogen #H3570) following manufacturer’s recommendations for antibody concentration and flow cytometry data acquisition on the BD LSR Fortessa ...
-
bioRxiv - Cell Biology 2021Quote: A pENTR-D clone of the full-length Pex14 open reading frame (Baron et al., 2016) was transferred to pDEST-17 (Thermo Fisher) using LR ClonaseII (Thermo Fisher 11791-020) ...
-
bioRxiv - Biophysics 2021Quote: ... in which endogenous hASIC1a was removed by CRISPR/Cas9 [17], were grown in monolayer in T75 or T175 flasks (Orange Scientific, Belgium) in DMEM (Gibco, Denmark) supplemented with 10 % FBS (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2019Quote: ... equipped with a 488 nm 20 mW blue laser and a 633 nm 17 mW red laser for exciting SYBR Green1 (Invitrogen S7563) and CTFR Proliferation (Invitrogen C34564 ...
-
bioRxiv - Microbiology 2020Quote: ... ISP2 and ISP4 (International Streptomyces Project medium 2 and 4; premixed Difco™ ISP2 dehydrated medium (Fisher Scientific, #DF0770-17-9) and premixed Difco™ ISP4 dehydrated medium (Fisher Scientific ...
-
bioRxiv - Bioengineering 2020Quote: ... The attached gold nanorod-coated liposome suspension was concentrated by centrifugation at 6000 rpm for 30 min to achieve a higher drug concentration (accuSpin Micro 17, Fisher Scientific). After centrifugation ...
-
bioRxiv - Cancer Biology 2020Quote: ... centrifuged at 4 °C and 12000 x g for ten minutes (Heraeus Fresco 17 Centrifuge Refrigerated, Thermo Scientific, Waltham MA, USA) to remove crude debris.
-
bioRxiv - Biochemistry 2022Quote: ... Cells were washed and collected by scraping and subjected to lysis in 1X PBS + 1% SDS as described in a previously reported protocol.17 Protein concentrations were determined using BCA assay (Thermo Scientific).
-
bioRxiv - Neuroscience 2022Quote: ... HEK293T/17 (ATCC Cat# CRL-11268, RRID:CVCL_1926, female) cells were routinely cultured in DMEM supplemented with 10% FBS (Gibco Cat#11995-065). Cell lines used were obtained from ATCC ...
-
bioRxiv - Neuroscience 2022Quote: ... Supernatants were then immunoprecipitated on a rotator for two hours at 4 °C with antibody pre-bound to 17 μl Protein A Dynabeads (Invitrogen, 10001D). The following antibodies was used ...
-
bioRxiv - Neuroscience 2022Quote: ... The supernatant containing the cells was filtered through 70-μm strainers into a tube containing 90% isotonic Percoll (GE Healthcare, #17-0891-01, in 10□×□PBS pH 7.2 (Gibco, #70013032). The final volume was topped up with DMEM/F12 (Gibco ...
-
bioRxiv - Immunology 2023Quote: ... iNOS AF488 (CXNFT, 53-5920- 82), iNOS APC (CXNFT, 17-5920-82) and iNOS PE (CXNFT, 12-5920-82) from Thermo Fisher, and CCR2 PE (475301 ...
-
bioRxiv - Developmental Biology 2022Quote: ... followed by FACS analysis with anti-CD11b/Mac-1 (M1/70)-APC (17-0112-81, eBioscience/Thermo Fisher Scientific; 1:400), anti-granulocyte mAb ...
-
bioRxiv - Microbiology 2023Quote: ... HEK-293T/17 cells were maintained at 30° C in a low-glucose formulation of DMEM (Cat # 12320–032; ThermoFisher Scientific), as previously described (22).
-
bioRxiv - Cancer Biology 2023Quote: ... tumor pieces are transferred to a 50-mL conical tube and 40 mL of 0.5% collagenase solution (Gibco; 17-100-017) in DKFSM media (Gibco ...
-
bioRxiv - Cancer Biology 2023Quote: ... with alignment performed using STAR 16 and quantification performed with Salmon.17 qRT-PCR was performed as previously described using TaqMan Universal Master Mix II with UNG (ThermoFisher Scientific, Applied Biosystems) and TaqMan Gene expression assays (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... All bacterial stocks were stored as aliquots at -80°C in brain heart infusion media (BHI; Fisher Scientific, DF0037-17-8) to minimize freeze-thaw cycles ...