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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Cell Biology 2023Quote: Copper-inducible pGL1 fly expression vectors for N-terminal FLAG- and HA-tagged cDNAs were generated by using EcoRI and XhoI restriction sites to insert the tag and SalI/NotI restriction sites from pRK5-FLAG or pRK5-HA vectors into a pMT-V5-His backbone (Life Technologies), followed by mutation of 2070C>A to remove a SalI site in the backbone ...
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bioRxiv - Cell Biology 2023Quote: ... was added with His-tag and inserted into pcDNA3.4 TOPO vector by pcDNA3.4 TOPO TA cloning kit (Thermo Fisher Scientific, MA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... and the His-tag was cut off overnight at 4 °C by the addition of 10 U AcTEV Protease (Thermo Fisher). ErCry4a was further purified using anion-exchange chromatography as previously described44 ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-protein complexes were purified with Pierce(tm) His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, #21277) with the following modifications ...
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bioRxiv - Systems Biology 2021Quote: ... The His-tagged proteins were purified on Ni-NTA resin (Thermo Scientific #88222) and washed into a buffer containing 50 mM Tris (pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
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bioRxiv - Microbiology 2022Quote: ... recombinant proteins were detected using primary anti-His antibody (ThermoFisher, Cat. # PA1-983B) and an anti-RNAP antibody (BioLegend ...
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bioRxiv - Biochemistry 2023Quote: ... The His-tagged protein was purified using HisPur Cobalt Resin (Thermo Fisher Scientific) in 20 mM Hepes (pH 7.3) ...
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bioRxiv - Cell Biology 2024Quote: ... His-tagged proteins were subsequently purified using Ni-NTA–agarose beads (Thermo Scientific) and elution buffer (20 mM HEPES ...
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bioRxiv - Immunology 2021Quote: Vero E6 cells (ATCC-CRL-1586, African Green Monkey) were maintained in DMEM (Gibco) supplemented with 2% fetal calf serum and 1% penicillin-streptomycin in a humidified incubator with 5% CO2 at 37°C.
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bioRxiv - Molecular Biology 2021Quote: ... African green monkey (Vero) cells were cultured in M199 medium (Gibco, Waltham, MA, USA) in a 37°C incubator with 5% CO2.
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bioRxiv - Biophysics 2022Quote: African green monkey kidney cells (COS-7) were cultured in DMEM-Glutamax (Gibco 10566016) supplemented with 10% FBS in a cell culture incubator (37°C and 5% CO2) ...
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bioRxiv - Microbiology 2023Quote: VeroE6TMPRSS2 (African green monkey kidney) cells were maintained in DMEM (Gibco cat. No. 11965) with 10% fetal bovine serum (FBS) ...
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bioRxiv - Plant Biology 2021Quote: ... “Protein extracted” with T7 tag antibodies were incubated with protein A containing magnetic beads (Dyna beads, Thermo fisher) for 2 hours at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were labeled with TMT10plex isobaric mass tag labeling reagents (Thermo Scientific), at a concentration of 20 μg/μL in dry acetonitrile ...
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bioRxiv - Immunology 2024Quote: ... Proteins were purified via CaptureSelect™ C-tag affinity matrix (Thermo Fisher Scientific). A further SEC polishing step was performed on a HiLoad 16/60 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Proteins from virus-infected cells were immunoprecipitated using protein G magnetic Dynabeads according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). Briefly ...
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bioRxiv - Biophysics 2021Quote: ... the protein construct was transformed into the Escherichia coli BL21 (DE3 Invitrogen) competent cells ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting proteins were expressed in Escherichia coli BL21(DE3) cells (Invitrogen) after induction by 0.5 mM isopropyl β-D-1-thiogalactopyranoside at 18°C overnight ...
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bioRxiv - Molecular Biology 2019Quote: ... GST fusion proteins were expressed in Escherichia coli BL21 Star (DE3) (Invitrogen) or C41 (86 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the protein was expressed in Escherichia coli BL21(DE3) cells (Life Technologies) using a pET-46 tobacco etch virus (TEV ...
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bioRxiv - Synthetic Biology 2023Quote: All recombinant proteins were produced in Escherichia coli BL21(DE3) pLysS (Invitrogen) transformed with respective plasmids ...
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bioRxiv - Immunology 2019Quote: ... adult females aged 6 to 11 days after eclosure were injected with fluorescent bioparticles (E. coli K-12 Strain Bioparticles TexasRed Conjugate; Invitrogen) or pH sensitive fluorescent bioparticles (pHrodo E ...
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bioRxiv - Microbiology 2019Quote: ... and POPG (1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1’-rac-glycerol) (Avanti Polar Lipid). LTA (Lipoteichoic acid from S. aureus) and LPS (lipopolysaccharide from E. coli) (both obtained from Invitrogen) were also tested ...
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bioRxiv - Cell Biology 2023Quote: ... differentiated monocytes were preincubated for 30 mins with PBS or 25 μμM Cytochalasin D and then incubated with pHrodo red E coli bioparticles (Thermofisher) with or without 25 μM cytochalasin D for 1 h 30 mins ...
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bioRxiv - Biophysics 2019Quote: ... the MerMAID1 gene was subcloned with a C-terminal TEV protease restriction site and a 6× His-Tag into the pPiCZ vector (Invitrogen, Carlsbad, CA). Zeocin™-resistant positive clones were selected from electroporation-transformed yeast cells ...
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bioRxiv - Cell Biology 2020Quote: S1R was expressed with an N-terminal 6*His-tag fusion in Sf9 cells using Bac-to-Bac baculoviral expression system (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
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bioRxiv - Biophysics 2022Quote: ... followed by an 8× His tag and HRV 3C protease cleavage site was cloned into the modified pFastBac1 (Invitrogen, Carlsbad, CA, USA) vector ...
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bioRxiv - Biochemistry 2021Quote: ... through G-25 resin the peptide-mRNA:cDNA libraries were incubated with cross-linked linked SARS-CoV-2 Mpro (200 nM) immobilized on Dynabeads™ His-tag Isolation & Pulldown (Life Technologies) at 4 °C for 30 min after which the beads were washed with ice-cold selection buffer (3 x 100 μL) ...
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bioRxiv - Cancer Biology 2020Quote: ... with C-terminal twin-Strep-10x His dual tag followed by a stop codon was synthesized using GeneArt Gene Synthesis service (Invitrogen, Carlsbad, CA) with SgfI and MluI restriction sites at the beginning and the end of the ORF sequence ...
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bioRxiv - Immunology 2021Quote: ... an HRV3C protease cleavage site, a TwinStrepTag and Hexa-His-tag (McLellan et al., 2020) was transiently expressed in FreeStyle293F cells (Thermo Fisher scientific) using polyethylenimine (PEI ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat. #MA1-21315-1MG, Thermo Fisher Scientific) in PBS ...
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bioRxiv - Immunology 2021Quote: ... The RBD-6xHIS protein was purified by affinity chromatography of His Tag residues for metals immobilized on the AktaPure system with HisPur ™ Ni-NTA Chromatography Cartridges (Thermo Fisher) column according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The ScPif1-N-His protein was labeled by binding to a 6x-His Tag Monoclonal Antibody (500 nM) conjugated to a DyLight™ 550 Fluorophore (Cat. # MA1-21315-D550, ThermoFisher Scientific).
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bioRxiv - Immunology 2022Quote: ... and cloned Pst I/Not I in a pPPI4 expression vector containing a hexahistidine (his) tag with Gibson Assembly (Thermo Fisher Scientific). All S constructs were verified by Sanger sequencing and the protein was subsequently produced in human embryonic kidney (HEK ...
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bioRxiv - Microbiology 2022Quote: ... or SARS-CoV-2 E protein (1 to 75 amino acids) (ThermoFisher Scientific) in coating buffer [0.05 M sodium carbonate-sodium bicarbonate (pH 9.6)] at 4°C for overnight incubation ...
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bioRxiv - Developmental Biology 2022Quote: ... or Alexa Fluor 647 conjugated F(ab)2 fragment swine anti-rabbit secondary antibody (Molecular Probes). After washing in Tris-buffer ...
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bioRxiv - Microbiology 2022Quote: His-GspD and His-PilQOlut were expressed in Escherichia coli BL21 cells and purified according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA). The proteins were injected into rabbits to generate polyclonal antibodies according to standard protocols (His-GspD ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged proteins were purified using HisPur Ni-NTA resin (Thermo Fisher Scientific), at RT ...
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bioRxiv - Immunology 2021Quote: ... and the protein was purified on His-Pur Ni-NTA resin (Thermo Fisher Scientific) followed by size-exclusion chromatography ...
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bioRxiv - Immunology 2020Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher). To eliminate nonspecific binding proteins ...
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bioRxiv - Biochemistry 2022Quote: ... His-tagged protein was labeled with Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) per manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... The His-tagged dAsapPZA protein was expressed and purified following manufacturer guidelines (Invitrogen, USA).
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bioRxiv - Microbiology 2024Quote: ... the His-tagged protein was purified using the ProBond Purification System (Thermo Fisher Scientific) and the eluted protein was dialyzed against PBS ...
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bioRxiv - Microbiology 2021Quote: African green monkey kidney (Vero) cells (Nuvonis Technologies) were maintained in OptiPRO SFM (Life Technologies) containing 2X GlutaMAX (Gibco) ...
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bioRxiv - Microbiology 2021Quote: African green monkey kidney (Vero) cells (Nuvonis Technologies) were maintained in OptiPRO SFM (Life Technologies) containing 2X GlutaMAX (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... and African green monkey kidney Vero E6 cells were maintained in DMEM (Gibco; #11995-065) containing 10% FBS and 1X non-essential amino acids ...
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bioRxiv - Microbiology 2021Quote: ... Spike protein (C9-tag) on the surface of pseudovirus was detected using a mouse anti-C9-tag monoclonal antibody 1D4 (Invitrogen, Cat. No. MA1-722), and an HRP-conjugated goat anti-mouse IgG Fc secondary antibody (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... The proteins were purified via CaptureSelect™ C-tag affinity matrix (Thermo Fisher Scientific). A further SEC polishing step was performed on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare ...