Labshake search
Citations for Thermo Fisher :
301 - 350 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... on QuantStudio 6 (Life Technologies). Primers were pre-validated for high efficiency and internally tested not to vary upon OP9 differentiation ...
-
bioRxiv - Microbiology 2022Quote: ... 6 days for blasticidin (Gibco), zeocin (Invivogen ...
-
bioRxiv - Biochemistry 2023Quote: ... 6% polyacrylamide gel (Invitrogen, EC68655BOX) and stained with SYBR-Gold (Invitrogen ...
-
bioRxiv - Biochemistry 2023Quote: ... 6% polyacrylamide gels (Invitrogen, EC68655BOX) to run in TBE (VWR ...
-
bioRxiv - Cell Biology 2023Quote: ... 6 mM L-glutamine (Gibco), 25 µg/mL G418 Sulfate (Gibco)] ...
-
bioRxiv - Microbiology 2023Quote: ... 6 mM L-glutamine (Gibco), and 100 mg/ml penicillin and streptomycin (P/S ...
-
bioRxiv - Cell Biology 2023Quote: ... 6 µL Lipofectamine 2000 (Invitrogen) and 2 µg plasmid ...
-
bioRxiv - Molecular Biology 2024Quote: ... 6 × loading buffer (Thermo Scientific) was added and the reaction mixture was separated on a 1% agarose gel in 1x TAE (40 mM Tris ...
-
bioRxiv - Biophysics 2020Quote: All the in vitro transcription experiments were performed in NEB’s transcription buffer (40 mM Tris-HCl, 6 mM MgCl2, 1 mM DTT, 2 mM spermidine) with 1 mM NTPs (R1481, Thermo Scientific), 22 wt% glycerol ...
-
bioRxiv - Cancer Biology 2021Quote: ... Culture medium was refreshed every 2–3 days and organoids were passaged 1:2–1:6 every 7–21 days using TrypLE Express (Thermo Fisher). For co-culturing ...
-
bioRxiv - Microbiology 2020Quote: ... just-subconfluent T150 flasks were transfected with 8.75 μg of HIV-1 YU2 or HIV-1 YU2 lacking Vpr (HIV-1 YU2 ΔVpr) and 30 μl Fugene 6 in 500 μl Optimem (Thermofisher Scientific). To make VSV-G pseudotyped HIV-1 GFP ...
-
bioRxiv - Immunology 2020Quote: PBMC (1-2*10^6 cells) and pDC (1-4*10^4) were maintained for 16 h in RPMI1640+10% FBS+ 1% (PS) (Gibco Laboratories) in 96 well round bottom plates ...
-
bioRxiv - Biochemistry 2020Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes).
-
bioRxiv - Developmental Biology 2021Quote: ... samples were washed in block six times for 1 hour and incubated in secondary antibodies conjugated to fluorescent dyes diluted 1:250 in block with added 4’,6-diamidino-2-phenylindole (DAPI; 1:500 dilution of 1 mg/ml stock, Thermo Fisher) for 2 nights at 4°C.
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... interleukin-6 and -8 (IL-6 and IL-8) human Instant Elisa™ kits and matrix metalloproteinase-1 (MMP-1) human Elisa kit from ThermoFisher Scientific.
-
bioRxiv - Cell Biology 2022Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes). Cells were imaged using a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
-
bioRxiv - Neuroscience 2023Quote: ... Rosettes were plated on growth factor reduced matrigel coated 6 well plates in ‘pNPC medium’ composed of a 1:1 mixture of Neurobasal (ThermoFisher Scientific) and DMEM/F12 ...
-
bioRxiv - Neuroscience 2021Quote: ... slices were incubated with 4’,6-diaminodino-2-phenylindole (DAPI, Life Technologies D-21490, 1:2000) for 15 min ...
-
bioRxiv - Neuroscience 2020Quote: ... The nuclear dye 4’,6-diamidino-2-phenylindole (DAPI) at 1 l ng/ml (Molecular Probes) was added to visualise all cells ...
-
bioRxiv - Physiology 2020Quote: ... Cell nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; ThermoFisher, Table S13). Confocal images were acquired using an inverted laser scanning confocal microscope (Nikon Eclipse C1) ...
-
bioRxiv - Neuroscience 2019Quote: ... Nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole, 1:500, Molecular Probes, Eugene, USA) and images were obtained using a scanning confocal inverted microscope (TCS ...
-
bioRxiv - Immunology 2021Quote: ... Nuclei were counterstained with 1/2500 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, ThermoFisher Scientific, USA). The cover slips were fixed in fluorescence mounting medium (Fluoromount-G ...
-
bioRxiv - Immunology 2021Quote: ... Nuclei were counterstained with 1/2500 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, ThermoFisher Scientific, USA). The cover slips were fixed in fluorescence mounting medium (Fluoromount-G ...
-
bioRxiv - Cell Biology 2019Quote: ... DNA is stained with 1 mg/ul 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher, Carlsbad, CA) 1/10,000 in PBS for 5 minutes and Vectashield mounting medium is applied (Vector Labs ...
-
bioRxiv - Cell Biology 2019Quote: ... DNA is stained with 1 mg/ul 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher, Carlsbad, CA) 1/10,000 in PBS for 5 minutes and Vectashield mounting medium is applied (Vector Labs ...
-
bioRxiv - Bioengineering 2019Quote: ... Sections were also counterstained with 4′,6-diamidino-2-phenylindole (DAPI; 1:1000; Invitrogen, cat. #D1306). Immunocytochemical images were acquired with a confocal microscope (Olympus FV1000 Laser Scanning Confocal ...
-
bioRxiv - Cell Biology 2019Quote: ... also confirmed by 4′,6-diamidino-2-phenylindole fluorescent dye (DAPI, 1:1000, Molecular Probes, USA) staining.
-
bioRxiv - Immunology 2021Quote: ... Chamberslides were then incubated in 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (1:5,000, ThermoFisher Scientific) in 1x PBS for 5 min ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were seeded (1 x 105 cells/ml) on gelatinised 6 well plates (Nunc, Roskilde, Denmark) and grown to ~80 % confluency ...
-
bioRxiv - Immunology 2020Quote: ... cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, Karlsruhe, Germany, 1:100) for 30 min at RT ...
-
bioRxiv - Genomics 2021Quote: ... enzymatic dissociation was performed for 4–6 minutes at 37°C in 1 mL TrypLE (Invitrogen), then cell pellets were washed with ice-cold PBS and lysed with 1 mL of TRIzol (Invitrogen) ...
-
bioRxiv - Cancer Biology 2022Quote: ... and 4′6-diamidino-2-phenylindole (DAPI, nuclei staining, 1:10000; Cat. no. D1306, Life Technologies) diluted in PBS for 30 min at RT ...
-
bioRxiv - Cancer Biology 2022Quote: ... Slides then were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) or with YOYO-1 (ThermoFisher). When HPV probe signal co-localized with YOYO-1 signal detecting DNA at 63x magnification ...
-
bioRxiv - Bioengineering 2022Quote: ... counterstained with 1 μg/mL of 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific, #D1306) for 30 minutes ...
-
bioRxiv - Immunology 2023Quote: ... Slides were washed and stained with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, D3571, 1:4000) in PBS for 10 min at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... enzymatic dissociation was performed for 4–6 min at 37 °C in 1 ml TrypLE (Invitrogen), then cell pellets were washed with ice-cold PBS and lysed with 1 ml of TRIzol (Invitrogen) ...
-
bioRxiv - Cell Biology 2022Quote: ... with 1 µl of the newly synthesized cDNA on the QuantStudio 6 Flex instrument (Applied Biosystems) in a 96 well format ...
-
bioRxiv - Biochemistry 2020Quote: ... After transfer and blocking of polyvinylidene fluoride (PVDF) membranes (Immobilon) in 5% BSA TBS Tween-20 buffer (Thermo Fisher Scientific), primary antibody directed against FADS2 (PA5-87765 ...
-
bioRxiv - Microbiology 2021Quote: ... Gels were run as per manufacturer prior to blotting onto a 0.2 µm polyvinylidene fluoride (PVDF) membrane using iBlot® system (Invitrogen). To enable the detection of HSP101 ...
-
bioRxiv - Microbiology 2023Quote: ... pH=7.5) and a cocktail of protease inhibitors (pepstatin A, leupeptin hemisulfate, aprotinin from Goldbio; phenylmethylsulfonyl fluoride from Acros Organics), bovine pancreas DNAase I (Goldbio) ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were spun down at 5000 rpm and resuspended in lysis buffer (300 mM NaCl, 50 mM Hepes, pH 7.0) with 100 mM phenylmethylsulfonyl fluoride (PMSF) (Thermo Scientific) and 0.2 U/mL DnaseI (Thermo Scientific) ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... Gels were transferred to a polyvinylidene fluoride (PVDF) membrane using the iBlot 2 Dry Blotting System (Thermo Fisher Scientific Inc). Membranes were stained with Ponceau S red solution (Fluka Analytical ...
-
bioRxiv - Microbiology 2020Quote: ... 5-(and-6)-carboxyfluorescein (FAM) and 5-(and-6)-carboxytetramethylrhodamine (TAMRA) were obtained from Invitrogen™ ...
-
bioRxiv - Plant Biology 2023Quote: ... 6 uL of 0.5 M EGTA (bioWORLD) and 6 uL of 0.5 M EDTA (Invitrogen) were added to quench the reaction at 65 °C for 10min ...
-
bioRxiv - Developmental Biology 2022Quote: ... for 5 minutes at RT and then split with 1:8 ratio into 6 well plates pre-coated with 1:200 diluted geltrex in DMEM/F12 medium (Thermo Scientific A1413201) for 30 minutes at 37C ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: HEK293T cells were seeded at the density of 106 cells/well in a 6-well plate and were grown overnight in complete DMEM/F12 1:1 Glutamax ™ (Thermofisher, UK). On the next day ...
-
bioRxiv - Zoology 2023Quote: ... stained with 4′,6-diamidino-2-phenylindole (DAPI) (1:2000 dilution) and Alexa Fluor-conjugated donkey-anti-mouse (Molecular Probes, 1:1000), in PBST at room temperature for 2 hours ...
-
bioRxiv - Zoology 2024Quote: ... Tissue samples were subsequently rinsed three times with DPBS again and then transferred onto microscope slides in mounting buffer containing 1:1 DPBS: glycerol and 1µg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Molecular Probes, Eugene, OR) to stain cell nuclei in prepared tissues and examined under a Lumen Dynamics XCite™ 120Q Nikon fluorescence microscope (Nikon ...
-
bioRxiv - Cancer Biology 2019Quote: ... and 5-6 pieces per well were cultured in a 6-well culture plate (Fisher Scientific). For up to two weeks tissue pieces were cultured in Advanced Dulbecco’s modified Eagle’s medium (DMEM ...
-
bioRxiv - Microbiology 2021Quote: ... using 6 mL of cell suspension and 6 mL of Prolong Gold Antifade mountant (ThermoFisher Scientific) that were mixed on an object glass and then covered with a special STED cover glass ...