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Citations for Thermo Fisher :
3401 - 3450 of 10000+ citations for Rat CCAAT Enhancer Binding Protein Alpha CEBPa Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the protein concentration was determined by means of a Pierce™ BCA protein assay (Thermo Fisher Scientific). For each sample the same amount of protein between 5 – 7.5 µg was loaded and separated by SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were assayed for protein concentration by the Pierce BCA protein assay kit (Thermo Fisher, Waltham, MA), and stored at −80°C prior to shipment to Vanderbilt University.
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were clarified by centrifugation and protein concentration was determined PierceTMBCA protein assay kit (Thermo Scientific). Samples containing equal amount of protein were resolved on 4-12% gradient SDS-PAGE gels and transferred to PVDF membranes ...
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bioRxiv - Neuroscience 2020Quote: ... and proteins were separated on NuPAGE®Novex® 4-12 % Bis-Tris Protein Gels (Life Technologies) followed by transfer onto a PVDF membrane (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... Complexes were captured with a 1:1 mixture of magnetic Protein A and Protein G Dynabeads (Invitrogen) for 3 h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal amounts of protein were separated on 4-12% Nupage Novex Bis-Tris protein gels (ThermoFisher Scientific). For ZFR immunoblots ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were identified by searching the data against Uniprot protein database using Proteome Discoverer (v2.4, Thermo Scientific). Search parameters included MS1 mass tolerance of 10 ppm and fragment tolerance of 0.2 Da ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration was determined by performing Pierce BCA Protein assay according to manufacturer’s instructions (Thermo Fisher Scientific). After Western blotting ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentrations were determined by a bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, Waltham, MA) and stored at −20°C until further handling.
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bioRxiv - Microbiology 2022Quote: ... The protein concentration of the supernatant was measured by Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). The total protein amount was adjusted and diluted with a 2× sample buffer ...
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bioRxiv - Genomics 2022Quote: ... The protein concentration of the lysates was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific). Immunoblotting was performed according to standard procedures ...
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bioRxiv - Synthetic Biology 2022Quote: ... the protein concentration of each lysate sample was measured by BCA Protein Assay Kit (Thermo Scientific, 23250) according to the manufacturer’s manual ...
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bioRxiv - Genetics 2022Quote: ... Supernatant containing protein was extracted and quantified using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, 23225). Protein was stored at -80 °C until needed ...
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Comprehensive profiling of antibody responses to the human anellome using programmable phage displaybioRxiv - Immunology 2022Quote: ... followed by a 4-hour IP with protein A- and protein G-coated magnetic beads (Dynabeads, Invitrogen). A first PCR was performed to amplify the peptide inserts ...
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bioRxiv - Immunology 2022Quote: ... The protein concentration was determined using a BCA protein assay with BSA as the standard (Thermo Fisher). Recombinant RelMtb has been shown previously to retain (p)ppGpp synthesis and hydrolysis activities and can serve as an antigen to measure RelMtb-specific T-cell responses ex vivo (3 ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was measured by using a BCA Protein Assay Kit (23225, ThermoFisher Scientific, Waltham, MA). Samples containing 20-50 µg protein were separated by SDS polyacrylamide gel electrophoresis and transferred to a nitrocellulose or polyvinylidene fluoride membrane (10600023 ...
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bioRxiv - Cell Biology 2022Quote: Native protein-protein interactions were stabilized by crosslinking with disuccinimidyl glutarate (DSG; Thermo Scientific, 20593 or A35392) as previously reported (15) ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein concentration was quantified using the Microplate Procedure with the Pierce BCA Protein Assay Kit (Thermo Scientific) according to the manufacturer’s instructions and used to calculate the concentration of the samples ...
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bioRxiv - Developmental Biology 2022Quote: ... 15 μL of protein A and 15 μL of protein G Dynabeads™ (Invitrogen 10002D and 10004D) were washed once with 1x PBST ...
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bioRxiv - Biophysics 2022Quote: ... Protein concentration was measured using a bicinchoninic acid (BCA) assay (Pierce BCA Protein Assay Kit, ThermoFisher Scientific), and 20 mg was boiled alongside sample loading buffer (Invitrogen NuPAGE LDS 4X ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by protein concentration estimation with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific Cat. # 23227). Approximately 40 μg of protein was run on a NuPAGE 4–12% Bis-Tris gel (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2019Quote: Protein concentrations of the samples were determined using the Pierce BCA Protein Assay Kit (Thermo Scientific 23227) according to the manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Protein concentration was determined using a BCA (Bicinchoninic acid) protein assay kit (#23228 and #23224, Thermo Scientific). The fluorimetric assay to assess the MAO-B activity was initiated by adding 100 μl of a reaction mixture containing Amplex Red reagent (400 μM) ...
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bioRxiv - Biochemistry 2019Quote: ... Protein concentration was measured by the bicinchoninic acid (BCA) protein assay (Pierce, Thermo Fisher Scientific, Northumberland, UK) in 1:20 dilutions of the resuspended pellets (final Triton X-100 concentration ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein lysate samples were run at 180v volts on NuPAGE 10% Bis-Tris protein gels from Invitrogen. Samples were blotted onto 0.2 µm nitrocellulose membrane and probed with p53 (clone DO1 ...
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bioRxiv - Neuroscience 2019Quote: ... Each sample’s total protein concentration was determined using Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA). The following ELISAs ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins contained in the eluted fractions were separated using NuPAGE™ 12% Bis-Tris protein gels (ThermoFisher) and transferred into nitrocellulose membrane as described previously ...
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bioRxiv - Cancer Biology 2019Quote: ... Two μL of resuspended protein sample were quantified using Pierce BCA protein assay kit (Thermo Fisher Scientific) according to the standard manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentration was quantified via bicinchoninic acid assay (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific, 23225) and Tecan i-control software pre-set BCA program ...
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bioRxiv - Neuroscience 2019Quote: Protein concentration was determined using a micro-BCA protein assay kit (Thermo-Fisher Scientific, Waltham, MA, USA). Prior to IB protein concentrations were corrected by silver stain (Silver Stain Plus™ kit ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentration was measured using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA; 23227). For Western blot analysis ...
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bioRxiv - Physiology 2019Quote: ... Gels were washed and proteins were stained with Imperial Protein Stain (Thermo Fisher Scientific, Rockford, IL, USA) for 2 hours ...
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bioRxiv - Synthetic Biology 2019Quote: ... The protein concentrations were measured using Pierce™ BCA Protein Assay Kit (Thermo Scientific, Cat no: 23225) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: The protein concentration of cell lysate was quantified using the Pierce 660 nm Protein Assay (Thermo Fisher).
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bioRxiv - Molecular Biology 2020Quote: ... Samples were loaded equally in terms of protein concentration into 10% Bis-Tris protein gels (Life Technologies) and blotted on nitrocellulose membranes ...
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bioRxiv - Developmental Biology 2020Quote: ... Prewashed beads (50% protein A and 50% protein G, catalog numbers 10002D and 10004D, respectively, Thermo Fisher) were added to the immunocomplex and incubated for 30 minutes at room temperature with rotation ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentration was quantified with a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, catalog no. 23225). Approximately 50 μg of total protein was loaded and separated on 12% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: OMVs were quantified by total protein dosage with the Pierce® BCA Protein Assay Kit (Thermo Scientific). OMVs were then analyzed by SDS-PAGE and immunoblotting ...
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bioRxiv - Neuroscience 2021Quote: ... Cell surface proteins were biotinylated and isolated using Pierce Cell Surface Protein Isolation Kit (#89881, Thermo Fisher). All the steps were performed at 4°C unless otherwise mentioned ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein extracts were clarified by centrifugation and then quantified using the Pierce BCA Protein Assay kit (ThermoFisher). 20μg of protein extracts were subjected to SDS-PAGE and then transferred onto a PVDF membrane ...
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bioRxiv - Bioengineering 2020Quote: Target proteins were immobilized to magnetic beads by first coating protein G magnetic beads (ThermoFisher Scientific, 10004D) with anti-Flag antibody (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... The fragmented chromatin was then pre-cleared with 5 μl of Dynabeads Protein A/Protein G (ThermoFisher) and immunoprecipitated overnight at 4 °C with the pre-prepared antibody-beads complex ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein concentrations were measured using the linearised Bradford method54 with Pierce Coomassie Protein Assay Kit (Thermo Scientific).
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bioRxiv - Synthetic Biology 2019Quote: ... and cell lysate protein concentrations were measured using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). The reaction mixture for CFPS consisted of the following components in a final volume of 8–400 μL containing 1.2 mM ATP ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration of cells was measured using a Micro BCA Protein Assay Kit (Thermo Scientific, Rockford, IL) and used to normalize cytokine levels with final concentrations adjusted to an equivalent of 25 μg of protein for comparison across experiments.
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bioRxiv - Neuroscience 2019Quote: ... and equal amounts of protein were then loaded on precast NuPage 10% Bis-Tris protein gels (Invitrogen). After electrophoretic separation ...
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bioRxiv - Neuroscience 2020Quote: ... Protein content was measured using the Pierce 660 nm protein assay reagent (Thermo Scientific, Waltham, MA, USA). Equal amounts of proteins were separated by denaturing electrophoresis in Mini-Protean TGX stain-free gels (Biorad ...
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bioRxiv - Biochemistry 2021Quote: ... Protein concentration of the clarified lysate was determined using Pierce BCA Protein Assay (Thermo Fisher Scientific, 23225), after which it was reduced by 100 mM dithiothreitol at room temperature for 30-60 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentration of protein-containing supernatant was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher). 10ug of each sample was loaded onto NuPage 4-12% Bis-Tris protein gels (Thermo Fisher ...
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Cwl0971, a novel peptidoglycan hydrolase, plays pleiotropic roles in Clostridioides difficile R20291bioRxiv - Microbiology 2021Quote: ... The intracellular total protein concentration was measured by using a BCA protein assay (Thermo Scientific, Suwanee, GA), and normalized to the same protein concentration for intracellular toxin analysis by ELISA ...