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Citations for Thermo Fisher :
3401 - 3450 of 10000+ citations for Puumala Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... The T cells were isolated using the EasySep human T cell isolation kit (Stemcell) and activated with human T-activator CD3/CD28 Dynabeads (Gibco) at a bead:cell ratio of 1:1 ...
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bioRxiv - Molecular Biology 2022Quote: In vitro sumoylation reactions were performed on protein array slides with more than 9000 human proteins spotted (ProtoArray® Human Protein Microarray v5.0, Invitrogen). The reaction was performed by the technical service of Invitrogen with our purified enzymes ...
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bioRxiv - Immunology 2022Quote: ... For Cytokine Multiplex of Human Inflammation Panel (Invitrogen™ Inflammation 20-Plex Human ProcartaPlex™ Panel) (Catalog # EXP20012185901, eBioscience/Invitrogen) procedures were performed following manufacturer protocol and the plate was processed on BioRad Bio-Plex 200 system with Bio-Plex-HTF attachment (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from human NSCLC cells and human clinical NSCLC tumor and normal samples by homogenization in Trizol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... supernatants were diluted 4-fold and assayed with the Amyloid beta 40 Human ELISA Kit and either the Amyloid beta 42 Human ELISA Kit or the Amyloid beta 42 Human ELISA Kit Ultrasensitive (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme-linked immunosorbent assays (ELISAs) for human IL-8 were performed using the Human IL-8 ELISA Kit (ThermoFisher SCIENTIFIC) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: HMF and MRC5 fibroblasts were transfected with a pool of 3 siRNAs targeting each of the 710 human kinases (Silencer Human Kinase siRNA Library, #A30079 Thermofisher). Fibroblasts (1250 cells/well ...
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bioRxiv - Immunology 2019Quote: ... human PBMC derived T cells were purified by Human T cell isolation kit from Invitrogen (Invitrogen Dynal AS, Oslo, Norway) according to manufacturer’s instruction ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were rinsed and re-suspended in flow buffer containing 25 μL mL−1 of each of the following monoclonal anti-human antibodies per sample: anti-human CD3-AF700 (56-0037-42, ThermoFisher), - CD40L/CD154-FITC (11-1548-42 ...
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bioRxiv - Immunology 2021Quote: ... and total T cells were purified from PBMC suspensions by negative immunomagnetic selection (purity >90%) using the Human Myeloid DC Enrichment Kit (STEMCELL) and the Untouched total human T cell (Invitrogen) kits ...
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bioRxiv - Cancer Biology 2022Quote: ... primary human breast CAFs were transfected with 75 nm human periostin-targeting stealth siRNA (si-POSTN) (ThermoFisher siRNA ID: HSS116400) or 75 nm non-targeting control siRNA (si-Control ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™ ...
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bioRxiv - Microbiology 2022Quote: ... Surface PCDH1 was stained using human anti-EC7 mAb-3677 (5 µg/mL) followed by anti-human Alexa FluorTM 555 (ThermoFisher) for 1 h at 4°C each ...
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bioRxiv - Molecular Biology 2023Quote: ... human ETAR (residues 21-406) and human ETBR (residues 27-424) DNA was cloned into a modified pFastBac vector (Invitrogen), which contains an N-terminal FLAG tag (DYKDDDD ...
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bioRxiv - Neuroscience 2023Quote: ... and secreted signaling proteins were measured in culture supernatants by the Human Immune Monitoring Center (HIMC) at Stanford University using a Human 48-plex Luminex Procarta Immuno-assay (ThermoFisher).
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bioRxiv - Neuroscience 2023Quote: ... The human FUS or human TARDBP cDNA was subcloned into the pENTR™/D-TOPO® vector (Thermo Fisher Scientific). To generate the Gateway destination vector pUAST-DEST ...
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bioRxiv - Neuroscience 2023Quote: Human colorectal adenocarcinoma (ATCC, #SK809) and human pancreatic carcinoma (ATCC, #CRL1469) cells were grown in McCoy’s 5A medium (Gibco, #16600082) and Dulbecco’s Modified Eagle’s medium (Gibco ...
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bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
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bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
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bioRxiv - Molecular Biology 2024Quote: ... Adult human dermal fibroblast (HDFa) cells (Cascade Biologics C-013-5C) were maintained in Human Fibroblast Expansion Basal Medium (Gibco) supplemented with Low Serum Growth Supplement (Gibco).
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of 100 μM oligonucleotide bridge 2 and 2 units of T4 PNK (ThermoFisher) were added to 20 μl of the purified smDNA solution ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA encoding the short transcriptional variant of human DA D2 receptor with an N-terminal FLAG tag (SF-D2R-S) was inserted into mammalian expression vector pcDNA 3.1(+) (Invitrogen)62 ...
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bioRxiv - Genetics 2020Quote: ... To generate donor plasmids the desired homology arm’s regions and epitope tag (either V5 or FLAG) were synthetized by Invitrogen GeneArt Gene Synthesis and cloned into the vector backbone pMK-RQ ...
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bioRxiv - Molecular Biology 2021Quote: ... and C-terminal foldon trimerization motif followed by an octa-histidine tag was cloned into a pcDNA3.1 expression vector (Invitrogen). Further ...
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bioRxiv - Molecular Biology 2020Quote: ... vector using Gateway cloning in frame with the C-terminal V5 tag (Gateway LR Clonase II mix; Life Technologies). The Gli2 P1-6A mutant was previously described (15) ...
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bioRxiv - Molecular Biology 2021Quote: ... and the C-terminal foldon trimerization motif followed by an 8×His-tag was cloned into the pcDNA3.1(+) expression vector (Invitrogen). Furthermore ...
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bioRxiv - Developmental Biology 2019Quote: ... GenBank accession number BC057894.1) with an N-terminal hemagglutinin (HA) tag were reverse transcribed with SuperScript III Reverse Transcriptase (Invitrogen) and PCR-amplified using Phusion high-fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Cancer Biology 2020Quote: Desalted peptides from each sample (500μg) were labeled with 10-plex Tandem Mass Tag (TMT) reagents (ThermoFisher Scientific, 90110) (58) ...
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bioRxiv - Molecular Biology 2019Quote: ... These sequences and corresponding WT sequences were polymerase chain reaction (PCR) amplified (with the inclusion of a FLAG tag for REL sequences) and inserted into the pcDNA3.1(+) vector (Invitrogen) and pTRE-TIGHT vector (Takara Bio) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... extended with a nucleic acid sequence encoding for 6 histidine residues (His-tag) and cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen). The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 µg of total peptides per condition were labeled with tandem mass tag (TMT10plex and TMT11, Thermo Fisher Scientific). After >98% TMT incorporation rate was confirmed ...
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bioRxiv - Biophysics 2021Quote: Full-length HA-tagged p75NTR and full-length Myc-tag TRADD were expressed from a pcDNA3 vector backbone (Invitrogen). The HA-p75E345A ...
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bioRxiv - Biophysics 2021Quote: ... as specified by the commercial protocol) and a fluorescent tag (Alexa Fluor® succinimidyl esters, Invitrogen™, Molecular Probes® ...
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bioRxiv - Neuroscience 2020Quote: ... and both WT and mutant VAMP2 sequences were shuttled into Gateway™ pDEST™ 26 Vector (6xHis tag, Invitrogen). The in-frame V5-STX1a and 6xHis-(m)VAMP2 sequences were verified by DNA sequencing.
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bioRxiv - Immunology 2021Quote: ... Cell pellets were then incubated with a mouse Anti-V5 tag monoclonal antibody (1.2 mg mL−1; Thermofisher Scientific) in PBS 0.1% BSA for 45 min at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasma triacylglycerol (TAG) and cholesterol were measured with colorimetric assays (TR22421 and TR13421, respectively, ThermoFisher Scientific, Middletown, VA, USA). Plasma ALT and AST concentrations were measured with kinetic spectrophotometric assays (A524-150 and A559-150 ...
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bioRxiv - Cancer Biology 2022Quote: ... RNAs were labeled with a desthiobiotin tag by using a Pierce RNA 3′ End Desthiobiotinylation Kit (20163; Thermo Scientific). Pull-down of RNA– protein complexes was performed using a Pierce™ Magnetic RNA-Protein Pull-Down Kit (20164 ...
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bioRxiv - Biochemistry 2022Quote: Bait and tag proteins (10-15 μg in 100 μL PBS) were immobilised in 96-well MaxiSorb plate (NUNC) o.n ...
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bioRxiv - Immunology 2022Quote: ... and C-terminal foldon trimerization motif followed by hexa-His tag) were used to transiently transfect Expi293F cells (Gibco). Four days after transfection ...
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bioRxiv - Biophysics 2022Quote: The human wild-type S1PR5 gene (UniProt ID Q9H228) with an N-terminal 3 × HA epitope (YPYDVPDYA) tag was cloned into pcDNA3.1+ (Invitrogen) at KpnI(5’ ...
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bioRxiv - Plant Biology 2022Quote: ... fused with an N-terminal 6×His-tag and cloned into expression vector pPIC9 (Thermo Fisher Scientific, California, USA). Correctness of the resulting constructs was confirmed by DNA sequencing prior to introduction into Pichia pastoris strain GS115 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Protein expression was confirmed by western blot using a 6x His-Tag HRP conjugated Monoclonal Antibody (Thermo Fisher Scientific). Once verified ...
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bioRxiv - Biochemistry 2021Quote: ... labelled with Tandem Mass Tag (TMT) six or ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled.
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bioRxiv - Microbiology 2021Quote: ... a recodonized TgHypo cDNA sequence fused to a C-terminal 3×HA tag was synthesized by GeneArt (Life Technologies). This fragment was cloned into the BamHI and HindIII sites of a modified version of the parental plasmid p5RT70loxPKillerRedloxPYFP-HX3 ...
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bioRxiv - Microbiology 2020Quote: ... which also encodes 3x Flag tag was first cloned into a pBS SK vector (Thermo Fisher, Waltham, MA USA). The pEPkan-S plasmid was used as a source of the kanamycin cassette ...
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bioRxiv - Biochemistry 2021Quote: ... the membranes were incubated 1 mg L-1 of anti-6X-His tag monoclonal antibody [HIS.H8] with an HRP conjugate (ThermoFisher) suspended in 10 mL 1X TBST for 0.5 hours ...
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bioRxiv - Cancer Biology 2020Quote: The trigger peptides required for the TOMAHAQ method were generated by labeling with TMT-super heavy tags (TMTsh, +235.18, ThermoFisher) on the AssayMAP Bravo ...
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A quantitative tri-fluorescent yeast two-hybrid system: from flow cytometry to in-cellula affinitiesbioRxiv - Biochemistry 2019Quote: ... A second copy of the Tag-BFP (primSB_0120 and 121) was inserted in our new NheI site (Thermo Scientific), by homologous recombination to generate the pBFP2 plasmid ...
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bioRxiv - Biophysics 2020Quote: ... were cloned into the plasmid pCellFree_03 for expression with N-terminal GFP tag.30 Each plasmid (60 nM) was added to Leishmania tarentolae extract31,32 supplemented with RnaseOUT (1:1000, Invitrogen) and the mixture was incubated at 28 °C for 2.5 h.
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bioRxiv - Cell Biology 2020Quote: ... nucleotide fragments encoding the fusion Flag tag protein and full-length of Myocardin and encoding the fusion Myc tag protein and full-length of STAT3 were cloned to mammalian expression vector pCDNA3.1 (Invitrogen) (Flag-pCDNA3.1-Myocardin ...