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Citations for Thermo Fisher :
3401 - 3450 of 10000+ citations for Anti NKCC1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... blocked and incubated with Anti-Loqs or anti-NS3 primary antibodies at a dilution of 1:200 followed by incubation with Alexa-Fluor 488 secondary antibodies (Invitrogen) at a dilution of 1:500 ...
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Epigenetic Interaction between UTX and DNMT1 Regulates Diet-Induced Myogenic Remodeling in Brown FatbioRxiv - Physiology 2020Quote: ... Fixed cells were incubated with anti-MyHC antibodies (MF20) overnight at 4°C and then incubated with anti-mouse secondary antibodies labeled with Alexa fluor 594 (Invitrogen) for 1 hour at room temperature and counterstained with DAPI ...
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bioRxiv - Neuroscience 2021Quote: ... followed by a 2 hour room temperature incubation in 10% NGS/90% antibody buffer containing the following fluorescently-conjugated secondary antibodies: Goat anti-mouse Alexa 488 (1:500; Invitrogen) and goat anti-rabbit Alexa 594 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... Nunc MaxiSorp ELISA plates were coated overnight at 4°C with 50 ml of capture antibody (anti-6xHis antibody, clone HIS.H8; ThermoFisher Scientific) at 2 mg/mL in PBS ...
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bioRxiv - Immunology 2021Quote: ... as the primary antibody and a 1:4,000 dilution of goat anti-mouse alkaline phosphate conjugate as the secondary antibody (ThermoFisher Scientific). Each dilution was tested in triplicate and neutralization curves were graphed using GraphPad Prism.
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bioRxiv - Immunology 2022Quote: ... Secondary antibodies used: F(ab’)2-Donkey anti-Goat IgG cross absorbed PE secondary antibody (Thermo Fisher Scientific Cat: 31860), anti-mouse APC secondary antibody (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Proteins were detected on a Li-Cor Odyssey Blot Imager using an anti-Caspase-11 primary antibody (cone 17D9) and Alexfluor-680 conjugated secondary antibody (Invitrogen).
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bioRxiv - Molecular Biology 2022Quote: ... equilibriated in 1X PBS were used to coat with anti-TLK1 or IgG isotype antibody (0.5μg antibody per reaction, Thermofisher, cat# 720397). To ensure the unfirom coating of beads ...
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bioRxiv - Microbiology 2022Quote: ... and with and Immunofluorescence using anti-rabbit GFP antibody (a gift from Jason Mercer, University of Birmingham, UK) and secondary anti-rabbit Alexa Fluor 488 antibody (Invitrogen). Nuclear staining was done by incubating the sections in Hoechst 33342 (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... for 1hr before being incubated overnight at 4°C in and primary antibodies (1:1000 mouse anti-V5 Tag monoclonal Antibody (Invitrogen) and anti-Actin monoclonal antibody (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed 3 times in TBS for 10 min each and incubated for 1 h at room temperature with a combination of 2 fluorescent dye-labelled secondary antibodies: a Cy3-conjugated goat anti-rabbit antibody (1:200; Invitrogen, Waltham ...
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bioRxiv - Microbiology 2022Quote: ... Spike protein was detected by primary antibody (1:1500, Sinobiological) followed by Alexa Fluor 647 anti-rabbit secondary antibody (1:1000, Invitrogen). The nucleus was labelled using DAPI (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 647-tagged goat anti-guinea pig antibody (catalog no.A21450, Thermo Fisher Scientific, USA), after which the sections were rinsed ...
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bioRxiv - Physiology 2022Quote: ... Hearts were then washed trice in PBS for 20 minutes and incubated with the secondary antibody (donkey anti-rabbit antibody conjugated to Alexa 555; A-31572, Invitrogen) that was diluted 1:100 for at least 2 days at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Primary antibody binding was detected by a goat-anti-rabbit antibody conjugated to horseradish peroxidase (HRP) (1:2000 dilution; ThermoFisher). Signals were detected using a chemiluminescence substrate and the Azure c600 Imaging System (Azure Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated with one of the following secondary antibodies at RT for 2 hours: anti-rabbit Alexa Flour-488 conjugated antibody (Cat# A-11008, Invitrogen), anti-rabbit Alexa Flour-555 conjugated antibody (Cat# A-21428) ...
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bioRxiv - Molecular Biology 2024Quote: ... The secondary antibodies used included Alexa Fluor 488-conjugated donkey anti-mouse IgG antibody (1:500, #A-21202, ThermoFisher Scientific), Alexa Fluor 488-conjugated donkey anti-rat IgG antibody (1:500 ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... incubated with primary antibody (anti-human ASCT2 from MedImmune diluted 1:3000 in FACS buffer) for 30 min and secondary antibody for 20 min (Goat anti-human IgG Fc PE secondary antibody (Invitrogen) diluted 1:250 in FACS buffer) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Blots were washed with TBST 3x for 5 min and incubated in corresponding secondary antibody for 1 hour room temperature: goat anti-rabbit antibody (Invitrogen) or rabbit anti-mouse antibody (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... they were incubated for 2h with the secondary antibody (Alexa Fluor 488-conjugated donkey anti-mouse antibody, 1:1000; Invitrogen). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... slices were stained with fluorescent secondary antibodies (Alexa Fluor 488 goat anti-rabbit secondary antibody, A-11008, Invitrogen, RRID: AB_143165) and DAPI at 1:500 dilution at 4°C for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Antibodies were labeled with appropriate host species secondary antibodies (Table S5): donkey anti-mouse IgG conjugated to Alexa 488 (Thermofisher, Cat No ...
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bioRxiv - Neuroscience 2024Quote: ... The following secondary antibodies were used in the study: donkey anti-goat antibody Alexa Fluor 647 (1:200, Invitrogen™), donkey anti-rabbit Alexa Fluor 546 (1:200 ...
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bioRxiv - Microbiology 2024Quote: ... Infected cells were then fixed for 20 min in 10% formalin and processed for immunofluorescence with 1 μg/ml mouse anti-RhCMV pp65B or 5 ug/ml mouse anti-Rh152/151 monoclonal antibody followed by a 1:500 dilution of goat anti-mouse IgG-Alexa Fluor 488 antibody (Invitrogen). Nuclei were stained with DAPI for 10 min (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... primary antibody for 1 h followed by Alexa Fluor 647 goat anti-rabbit secondary antibody (1:1000; Cat. A21245, Invitrogen) for 1 h to label the CCVs ...
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bioRxiv - Immunology 2023Quote: ... sections were incubated for 45[min at room temperature with the following secondary antibodies: CD41 was visualized with the antibody goat anti rat Alexa Fluor 488 (Catalog #A-11006, Invitrogen); the LSP1 ...
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bioRxiv - Developmental Biology 2023Quote: ... The primary antibodies were diluted 1:200 and the secondary anti-rabbit antibody (Alexa Fluor 594 or 488 coupled, Invitrogen) was diluted 1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... The following secondary antibodies were used here: Alexa Fluor 555-labeled donkey anti-rabbit secondary antibody (1:500, RRID: AB_162543, Invitrogen, USA), Alexa Fluor 488-labeled donkey anti-mouse secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then washed 3 times with Tween20 solution before a 1 h incubation with secondary antibody solution containing goat anti-mouse antibodies conjugated to AlexaFluor 647 (ThermoFisher), goat anti-rabbit antibodies conjugated to AlexaFluor 488 (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated in primary antibody diluted in blocking buffer using the following primary antibodies: anti-Phospho-Tau (Ser396) (1:500, 44-752G, Invitrogen), anti β-catenin (E-5 ...
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bioRxiv - Immunology 2023Quote: ... the pellet was resuspended in 90 μl of Secondary antibody solution (Blocking buffer + 1:500 Goat anti-rabbit IgG AlexaFluor 647 antibody [#A27040, Invitrogen]) and the samples were incubated at room temperature for 45 min in the dark ...
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bioRxiv - Microbiology 2023Quote: ... Each sample was blocked with 3% BSA in PBS then stained anti-β (1,3)-glucan antibody (Biosupplies Australia Pty Ltd., Australia) followed by secondary antibody of anti-Mouse IgG (H+L) Alexa Fluor 488 (ThermoFisher). The level of β-1,3-glucan exposure on the cell surface was then quantified on a Beckman Coulter CytoFlex 3-laser cytometers ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was transferred to a primary antibody solution with a 1:500 concentration of anti-parvalbumin antibody (Invitrogen, PA1-933), 2.5% normal goat serum ...
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bioRxiv - Neuroscience 2023Quote: ... as the primary antibody and anti-rabbit IgG antibody conjugated with Alexa Fluor 488 (goat, 1/400, #A11034, Molecular Probes) as a secondary antibody ...
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bioRxiv - Microbiology 2023Quote: ... The SARS-CoV-1 S protein was detected using the SARS-CoV-1 S rabbit polyclonal primary antibody (NR-4569, BEI resources) and Alexa Fluor 488-labeled goat anti-rabbit secondary antibody (Invitrogen). The resulting protein bands were visualized using a Chemidoc system with Image Lab image capture software (BioRad).
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bioRxiv - Developmental Biology 2023Quote: ... We washed the excess antibody solution by PBST and replaced the solution with a secondary antibody solution [anti–rabbit IgG Alexa Fluor 594 antibody (A11037, Invitrogen) diluted in 1/200] ...
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bioRxiv - Microbiology 2023Quote: ... 96-well plates were blocked with 2% BSA in PBS for 30 minutes and then stained with a 1:500 dilution of mouse anti-RhCMV pp65b antibody clone (VGTI Monoclonal Antibody Core) in PBS for 1 hour followed by a 1:1000 dilution of Alexa488-conjugated anti-mouse antibodies (Invitrogen) and 3 µM DAPI (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... the primary antibody was detected using a donkey polyclonal anti-rabbit IgG secondary antibody conjugated to Alexa Fluor 647 (Invitrogen) diluted 1:1000 in permeabilization buffer for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary Antibody (Cat # A10524) and Cy5 conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody (Cat # A10523) were purchased from Invitrogen.
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bioRxiv - Bioengineering 2023Quote: ... and then incubated with the secondary antibodies as follows: goat anti-rabbit IgG antibodies conjugated with Alexa 488 (1:500, Invitrogen) and donkey anti-mouse IgG antibodies conjugated with Alexa 594 (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... cells were incubated for 45 minutes in secondary antibody solutions with AF647 secondary antibodies (anti-mouse, 1:1000, A31571, Invitrogen or anti-rabbit ...
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bioRxiv - Bioengineering 2023Quote: ... wells were washed 3 times with PBS with 0.1% tween (PBST) and then incubated overnight at 4 °C with the anti-iNOS primary antibody (Rabbit polyclonal Antibody, PA5-1624, Invitrogen) at 0.002 ng/ml in 1% BSA in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... NF-L primary antibody was detected with a Alexa Fluor Plus 647-conjugated goat anti-rabbit IgG (H+L) secondary antibody (A32733, Invitrogen). Tissues were counterstained with DAPI and whole slide images were obtained using a Vectra Polaris slide scanner with a 40x objective (Akoya Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... incubated with anti-Col1a1 antibody (CST) and followed by Alexa-Fluor-488 conjugated secondary antibody (#A-11008; Thermo Fisher Scientific). All the slides were stained with the antifade reagent (with DAPI ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated with primary antibodies (Supplemental Table 3) followed by secondary antibodies (goat anti-rabbit IgG Alexa 488, 1:200, Invitrogen; goat anti-mouse IgG Alexa Fluor 594 ...
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bioRxiv - Neuroscience 2023Quote: ... The following secondary antibodies were used here: Alexa Fluor 555-labeled donkey anti-rabbit secondary antibody (1:500, RRID: AB_2762834, Invitrogen, USA), Alexa Fluor 488-labeled donkey anti-mouse secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... The following secondary antibodies were used here: Alexa Fluor 555-labeled donkey anti-rabbit secondary antibody (1:500, RRID: AB_162543, Invitrogen, USA), Alexa Fluor 488-labeled donkey anti-mouse secondary antibody (1:500 ...
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bioRxiv - Immunology 2023Quote: ... Infected cells were then fixed for 20 minutes in 10% formalin and processed for immunofluorescence with 1 μg/mL mouse anti-RhCMV pp65B or 5 ug/mL mouse anti-Rh152/151 monoclonal antibody followed by a 1:500 dilution of goat anti-mouse IgG-Alexa Fluor 488 antibody (Invitrogen). Nuclei were stained with DAPI for 10 minutes (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... as the primary antibodies and Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (diluted at 1:200, A-11008; Invitrogen) and Cy3-conjugated goat anti-mouse secondary antibody (diluted at 1:200 ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies consisted of goat and alpaca anti-rabbit antibodies conjugated with Alexa Fluor 488 (Molecular Probes, Thermo Fisher Scientific), Cy3 or Cy5 (Jackson ImmunoResearch) ...