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Citations for Thermo Fisher :
3401 - 3450 of 8578 citations for 7 CHLOROHEPTANOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The myotubes growth for 7 days in “Differentiation medium” (DMEM F12-Glutamax (10565-018, Gibco); 5% FBS (SH30070.02 ...
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bioRxiv - Microbiology 2021Quote: ... or with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) for 30 min at room temperature (1:150 in AnnexinV binding buffer-Biolegend) ...
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bioRxiv - Cancer Biology 2020Quote: ... Dead cells were excluded from the analysis with the viability dye 7-AAD (Life Technologies). ISCs were isolated as Lgr5-EGFPhiEpcam+ CD45−7-AAD− with a BD FACS Aria II SORP cell sorter into supplemented crypt culture medium for culture.
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bioRxiv - Biochemistry 2020Quote: ... The fluorogenic probe 7-diethylamino-3-(4’-maleimidylphenyl)-4-methylcoumarin (abbr. CPM, ThermoFisher, Cat# D346) in 100% DMSO was then added to both quench the enzymatic reaction and react with CoASH to yield the fluorescent CPM-SCoA complex for fluorescence quantification 27 ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative real-time PCR (RT-qPCR) was performed using the QuantStudio 7 Flex (Applied Biosystems) using TaqMan Fast Advanced master mix and TaqMan primer/probes (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... COS-7 cells were incubated with 250□nM MitoTracker Green FM (Thermo Fisher Scientific, M7514) in an HBSS medium (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: An Applied Biosystems ViiA™ 7 (Life Technologies, Inc., Applied Biosystems, Foster City, California, U.S.A.) quantitative thermocycler was used for eDNA amplification and quantification ...
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bioRxiv - Molecular Biology 2019Quote: Relative mRNA expression was measured by qPCR (QuantStudio 7 Flex, Applied Biosystems, Foster City, CA) using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Subcutaneous tumors were harvested and sectioned (7 μm) using an HM525 NX Cryostat (Thermo Scientific). The tumor tissue sections were fixed by 4% paraformaldehyde (PFA ...
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bioRxiv - Cancer Biology 2019Quote: ... 5(6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA; CA-DCF-DA; (C400, ThermoFisher Scientific)) at a stock concentration of 20 mM in DMSO was diluted in DMEM without phenol red to a concentration of 40 μM ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-E-cadherin (2.5 µg/mL, clone 36, BD Transduction Laboratories or SHE78-7, Invitrogen), anti-KI-67 (1:400 ...
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bioRxiv - Systems Biology 2019Quote: ... qPCR was performed on 384-well plates in a QuantStudio 7 Flex system (Applied Biosystems) using the default “fast” cycling conditions and a total reaction volume of 12μl ...
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bioRxiv - Plant Biology 2021Quote: qRT-PCR was carried out on the QuantiStudio 7 Flex RT - PCR system (Applied Biosystems) and relative gene expression was calculated as 2-(Ct Target gene – Ct housekeeping gene ...
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bioRxiv - Genomics 2019Quote: ... An ArrayControl RNA Spots and Spikes kit (with spike numbers 1, 4 and 7) (Ambion) were used to monitor technical variability ...
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bioRxiv - Biochemistry 2020Quote: ... on the QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems™, USA, 4485701). Primers were designed with the NCBI primer designing tool (primer-blast ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were run in duplicates in ViiA™ 7 Real-Time PCR System (Thermo Fisher). β-actin and 18S probes served as endogenous controls (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... 7 µl of commercially available PCR master mix (TaqMan Universal PCR Master Mix, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: C-LP cells pooled from 5-7 mice were sorted into TriZol® (Thermo Fisher) and cDNA was generated by using QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Microbiology 2019Quote: Huh-7 (human hepatoma) cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) containing 10% (vol/vol ...
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bioRxiv - Microbiology 2019Quote: LLC-MK2 cells (ATCC CCL-7) were maintained in minimal essential medium (MEM, Life Technologies) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2019Quote: The MCF 7 cells were cultured in RPMI 1640 media (ThermoFisher Scientific, cat. no. 11875093) supplemented with 10% Charcoal-stripped serum (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... was assessed by the cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (CFDA) (10 µg/ml, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... exchanged daily for 7 days and then substituted by STEP2 medium containing Neurobasal medium (Gibco) with 2 % MACS NeuroBrew-21 w/o Vitamin A (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2020Quote: Zebrafish RNA was isolated from 7 days postfertilization (dpf) embryos using Trizol (Thermo Fisher scientific). mRNA was reverse transcribed using polyT primers with the SuperScript™ III First-Strand Synthesis System (Thermo Fisher scientific ...
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bioRxiv - Physiology 2021Quote: ... Measurements were performed on a ViiA™ 7 Real-Time PCR System from Applied Biosystems. The following primer sequences were used ...
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bioRxiv - Biochemistry 2021Quote: ... Excess dye was removed with a Zeba™ 7 K MWCO spin desalting column (ThermoFisher) equilibrated with running buffer.
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bioRxiv - Biochemistry 2021Quote: ... 5-7 μg BACMID DNA was incubated with 4 μL Fugene (Thermo Fisher, Cat# 10362100) in 200 μL of ESF 921 media for 30 minutes at 23°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were stained with 2 μM propidium iodide (PI) and 7 μM Hoechst 33342 (ThermoFisher) for 5-10 min and imaged under a Zeiss Observer Z1 microscope ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 µl of ERCC RNA spike-in mix at 10:7 dilution (Life Technologies) and barcoded reverse transcription (RT ...
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bioRxiv - Neuroscience 2021Quote: ... Hippocampal neurons were transfected with sypHy at DIV 7 using Lipofectamine 2000 (ThermoFisher Scientific, 11668027) as per manufacturer’s instructions and imaged at DIV 13-15 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human Amphiregulin PE Cy 7 (1:25, clone AREG559, Invitrogen, cat # 25-5370-42), Anti-mouse Nur77 APC (1:25 ...
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bioRxiv - Immunology 2021Quote: ... LLC-MK2 cells (ATCC CCL-7) were maintained in minimal essential medium (MEM, Life Technologies) supplemented with 10% fetal bovine serum (Wisent ...
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bioRxiv - Molecular Biology 2020Quote: ... Biotinylated EVs were loaded onto Zeba spin desalting columns (7 K MWCO; Thermo Fisher Scientific) to remove the remaining free biotin ...
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bioRxiv - Immunology 2020Quote: ... according to the manufacturer’s instructions on a Viia 7 Real-Time PCR System (ThermoFisher Scientific). Expression levels were normalized to Actb (β-actin or Rpl32 (ribosomal protein L32) ...
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bioRxiv - Bioengineering 2021Quote: ... Quantitative PCR was performed using the QuantStudio 7 Flex Real-Time PCR System (ThermoFisher Scientific) using TaqMan Gene Expression Assays (ThermoFisher Scientific #4331182) ...
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bioRxiv - Cell Biology 2020Quote: HuH-7 and Hela cells were cultured in low glucose (5mM) DMEM (21331046, Thermo Fisher) supplemented with 10% heat inactivated FBS (10082147 ...
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bioRxiv - Genetics 2020Quote: ... 25 μL of media containing STS and CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) at final treatment concentrations of 1 μM and 2.5 μM ...
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bioRxiv - Genomics 2020Quote: ... Kidneys were cryosectioned at 7 micron thickness and mounted on Superfrost slides (Thermo Fisher Scientific). Sections were washed with PBS (3 times ...
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bioRxiv - Genetics 2020Quote: ... Capillary electrophoresis was performed on the 3130xl genetic analyzer using POP-7 (Thermo Fisher Scientific), and alleles were analyzed using GeneMaker v2.4.0 software (SoftGenetics ...
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bioRxiv - Cancer Biology 2020Quote: ... MCF-7 derivatives were developed and cultured in phenol red-free RPMI 1640 (Thermo Fisher) supplemented with 5% charcoal stripped fetal bovine serum (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... 7) rinsed with Milli-Q H2O and mounted with ProLong Diamond (Thermo Fisher Scientific, USA) and cured for 24-48 hours at room temperature.
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bioRxiv - Biophysics 2022Quote: ... The assay was run on QuanStudio 7 Flex Real-Time PCR systems (Thermo Fisher Scientific). Relative RNA levels were calculated using the ΔΔCT method and normalized to the level of housekeeping gene human HRPT1 within the same sample and further normalized to the sample from cells transfected with control empty vector ...
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bioRxiv - Biophysics 2022Quote: ... Unreacted biotin was removed with Zeba™ Spin Desalting Columns (7 MWCO, Thermo Fisher Scientific). The kinetic assay between DinB variants and RNAP core was carried out by immobilizing biotin-labeled proteins (0.5 μM Biotin-DinB ...
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bioRxiv - Biochemistry 2022Quote: Proteins were buffer exchanged using ZebaTM Spin Desalting Column (7 kDa MWCO, Thermo Scientific #89890) into 50 mM HEPES-KOH (pH 7.5 ...
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bioRxiv - Bioengineering 2022Quote: ... Excess reactants were removed via 7 kDa size exclusion chromatography (Zeba Spin, Thermo Fisher Scientific) in some experiments ...
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bioRxiv - Genetics 2022Quote: ... Real time PCR reactions were processed on a ViiA 7 RT-PCR system (Applied Biosystems). All reactions were standardized with peptidyl prolyl isomerase A (Ppia ...
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bioRxiv - Microbiology 2022Quote: ... Two-fold serial dilutions of each drug were prepared in RMPI+MOPS pH 7 (Gibco RPMI 1640 with L-glutamine [Cat# 11875-093] and 0.165M MOPS) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... After 7 days adherent macrophages were scraped from the petri dishes with versene (Gibco, 15040) and then plated into tissue culture grade plastic 96 well dishes and left for 24 h to re-attach before exposure to chemical compounds.
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bioRxiv - Neuroscience 2022Quote: ... The electrodes were labelled with DiIC18(7) (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindotricarbocyanine Iodide) (DiR) (Invitrogen) to enable post-mortem reconstruction of the electrode tracks in histological sections.
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bioRxiv - Physiology 2023Quote: ... followed by 7 days of decalcification with Shandon’s TBD-2 (Thermo Scientific, Waltham, MA, USA) at room temperature ...