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Citations for Thermo Fisher :
3351 - 3400 of 10000+ citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and from original strains (C2865, C2853, C2854, C2855) were digested with 1 Unit of S1 enzyme (1 min at 37°C) (Thermo Fisher Scientific) for plasmid linearization ...
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Guidelines for accurate genotyping of SARS-CoV-2 using amplicon-based sequencing of clinical samplesbioRxiv - Genomics 2020Quote: ... The number of viral genome copies for the strain specified in the experiment was spiked into 50 ng of Universal Human Reference RNA (ThermoFisher Scientific #QS0639) to the final volume of 11 μl ...
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bioRxiv - Biochemistry 2022Quote: ... The raw data was searched against the Mycolicibacterium smegmatis (strain ATCC 700084 / MC2 155) UniProt Reference Proteome (Proteome ID UP000000757) using Sequest HT (University of Washington and Thermo Fisher Scientific). Iodoacetamide-mediated cysteine carbamidomethylation was set as a static modification ...
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bioRxiv - Molecular Biology 2024Quote: Wild type genomic DNA of Mycobacterium tuberculosis strain H37Ra was obtained from ATCC (Cat#25177DQ) and quantified by Nanodrop spectrophotometer (Thermo Fisher Scientific). DNA fragments with wild type or mutation MTB DNA sequences were synthesized and cloned into pUC19 plasmid by GenScript (Figure 2) ...
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bioRxiv - Microbiology 2023Quote: ... with the resulting plasmid confirmed as correct by Sanger sequencing (Genewiz). The Escherichia coli (E. coli) DH5-α strain (Thermo Fisher Scientific) was used for plasmid amplification ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were strained through a 35 µm strainer and incubated at 37°C for 30 minutes with 0.5 µL Vybrant™ Dyecycle™ Ruby strain (Invitrogen, V10309) per mL of FANS buffer.
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bioRxiv - Molecular Biology 2023Quote: ... coli EPI300 strains were cultured at 37 °C and at 250 rpm in the Luria broth medium (Fisher Scientific, Pittsburgh, PA, USA).
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bioRxiv - Genetics 2023Quote: ... Drosophila MAGs were prepared by dissecting accessory glands from 50 fly abdomens (Canton-S strain) into 50 ul of PBS (Gibco, pH 7.2) and homogenized with a pellet pestle motor (Kimble) ...
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bioRxiv - Synthetic Biology 2022Quote: ... aureus (200 ng/mL each) were added into the mixed culture containing three DNA sensor strains in separate 14 mL Falcon tubes (Thermo Fisher Scientific). The DNA sensor strains were incubated at 37°C with shaking (250 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... The correct strains were selected on YPD agar plates supplemented with 200 mg/l of G418 (Thermo Fisher Scientific, cat no 10131019), and confirmed by PCR.
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bioRxiv - Genomics 2023Quote: ... Genomic DNA samples used for loading comprised around 50–150 ng per strain (measured by Qubit 4 Fluorometer; ThermoFisher Scientific, Waltham, USA). The sequencing ran for 48 hrs using the MinKNOW software versions 2.2 ...
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bioRxiv - Biochemistry 2023Quote: ... Digested scFv inserts were subcloned in pET22b(+) vector (EMD Biosciences) and transformed into BL21(DE3) E-coli strain (Thermo Fisher Scientific, USA). Amber stop codon in CDR regions of scFvs were mutated to glutamine by site-directed mutagenesis kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Triplicates of 200 uL for each strain cell culture were transferred into Nunc 96-well black/clear bottom plates (Thermo Scientific, 165305). mNG detection was performed using Ex ...
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bioRxiv - Microbiology 2023Quote: ... A 10 µl volume of phage lysate was added to a log-phase culture of the Klebsiella host strain in LB supplemented with 5 mM CaCl2 (Thermo Fisher Scientific) and 5 mM MgCl2 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Procyclic cells (PCF) derived from Trypanosoma brucei subspecies brucei strain Lister 427 were grown in SDM-79 JRH 57453 media (Life Technologies UK) supplemented with 7.5 mg/l of hemin (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2023Quote: ... after culturing of the strain in permissive medium and extraction of the genomic DNA by PureLink™ Microbiome DNA Purification Kit (ThermoFisher Scientific). Short read whole genome sequencing ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA of the cloned strain of marseillevirus was prepared from the supernatant of infected A.castellanii using PureLinkTM Genomic DNA Mini Kit (Invitrogen, Waltham, United States) following manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... were determined in each strain in aerobic or microaerobic conditions by staining with 20 μM of ROS-sensitive dye (H2DCFDA, Thermo Fisher Scientific) for 30 minutes covered from light ...
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bioRxiv - Microbiology 2020Quote: ... The total protein content was measured using the Pierce BCA Protein Assay kit (Thermo Fisher) and 7.5 µg of lysate was run on a 4-12% Bis-Tris sodium dodecyl sulfate (SDS)-polyacrylamide gel with MOPS running buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations were measured using BCA protein assay kit (ThermoFisher Scientific; Waltham, MA, United States) in accordance with manufacturer’s instructions and 10 μg of protein was loaded per lane (N = 3 - 4) ...
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bioRxiv - Cancer Biology 2021Quote: One mg protein sample was precleared with Dynabeads Protein A (Thermo Fisher, UK, Cat.100002D) and 1 μg isotype IgG2a (Cell Signalling ...
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bioRxiv - Cancer Biology 2021Quote: ... p53-associated protein were pulled down by 5 μl DynabeadsTM protein G beads (#10004D, Invitrogen) at 4 °C for 1-1.5 h ...
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bioRxiv - Cancer Biology 2021Quote: ... and the protein concentration was determined by a Pierce BCA protein assay (Life Technologies, #23225). Equal amounts of protein were loaded into 8% Bolt™ Bis-Tris Plus gels (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein quantification was performed using a Pierce Bicinchoninic acid (BCA) protein assay kit (Thermo Scientific) and 300 μg of proteins were used for O-glycans enrichment adapted as previously described (25) ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein levels were normalised across samples using a Pierce™ BCA protein assay kit (ThermoFisher) according to manufacturers instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was collected for protein concentration quantification with BCA Protein Assay Kit (ThermoFisher Scientific). An equal amount of protein from each sample was subjected to quantitative analysis of IL-8 and IL-1β using ELISA kit (R&D system ...
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bioRxiv - Cell Biology 2020Quote: ... the proteins were separated with the NuPAGE system (ThermoFisher, 4-12% Bis-Tris protein gel), and transferred to PVDF membrane (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: ... and the protein concentration was determined using the BCA Protein Assay Kit (#23227, Thermo Scientific).
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bioRxiv - Cell Biology 2020Quote: ... Total protein concentration was measured using a Pierce BCA protein assay kit (Thermo Scientific Fisher). After boiling the samples in Laemmli buffer at 95°C for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was estimated using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) according to manufacturer protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined with the Pierce 660nm Protein Assay (Thermo Fisher Scientific, cat# 2262). Proteins were resolved by standard SDS polyacrylamide gel electrophoresis using pre-stained PAGE RULER plus (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... Equal amounts of protein were loaded in NuPAGE bis-tris protein gels (Thermo Fisher Scientific) and electroblotted to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2020Quote: ... The protein concentration was assessed using the Pierce BCA Protein Assay kit (Thermo Fisher Scientific). The mitochondria were pelleted by centrifugation (14,000 x g ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration from rat plasma was quantified using bicinchoninic acid protein assay (ThermoFisher Scientific, USA). Samples were diluted with Laemmli SDS Sample Buffer and heated at 95-100°C for 5 min followed by centrifugation at 16,000 x g at 4°C for 2 min ...
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bioRxiv - Microbiology 2019Quote: ... The proteins concentration was determined using a BCA Protein Assay Reagent Kit (Thermo Fisher Scientific) and stored at −80°C.
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bioRxiv - Genomics 2020Quote: ... 5 µg antibody was coupled to 50 µl Protein A or Protein G Dynabeads (Invitrogen). The following antibodies were used ...
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was quantified using the BCA Protein Assay Kit (Fisher Scientific, Cat# 23227). Samples were normalized to a fixed amount of protein ...
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bioRxiv - Biochemistry 2022Quote: ... Total protein content was estimated using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... and 0.01% bromophenol blue) and protein concentrations were measured using Pierce 660nm Protein Assay (ThermoFisher).
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentrations were measured using Pierce ™ BCA Protein Assay Kit (23225; Thermo Scientific) with a Albumin BSA Standards ranging from 2000 μg/mL to 0 μg/mL using a Nanodrop Spectrophotometer cuvette system (ND-2000C ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were determined using the BCA protein assay kit (ThermoFisher Scientific, Rockford, IL, USA). 30 µg of WCE were separated on mini protein TGX stain-free gel and electroblotted using the PVDF transfer membrane (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... then protein concentration was measured with Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). 300 μg lysates were pre-cleared with 2 μl Protein A Dynabeads (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein-protein complexes were eluted in NuPAGE™ LDS Sample Buffer (4x) (Thermo Fisher Scientific) at 100 °C for 10 min.
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentration was quantified using the Pierce ™ BCA Protein Assay kit (Thermo Fisher Scientific). 30 µg of total protein was loaded onto a 10% polyacrylamide gel and transferred to a nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2020Quote: ... the protein quantity was measured using the Micro BCA protein assay kit (Thermo Fisher Scientific) and a SpectraMax microplate reader.
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration of cell lysate was measured using the Coomassie protein assay kit (Thermo Scientific). Proteins were separated on SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... and protein levels in the postnuclear supernatants measured with Pierce BCA Protein Assay Kit (Thermofisher). After equalizing protein amount and concentration in all samples ...
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bioRxiv - Immunology 2021Quote: ... Protein concentrations for each fraction were determined by the BCA protein assay kit (Thermo Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... and measured the protein concentration using the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific). For comparing DAgs of different deltaviruses ...
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bioRxiv - Cancer Biology 2019Quote: ... The cultured cells were treated to extract proteins using a protein extraction kit (Thermo Fisher), with bovine serum albumin as the standard ...