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Citations for Thermo Fisher :
3301 - 3350 of 10000+ citations for TIM 1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... four expression vectors were transiently transfected into Expi293 cells using ExpiFectamine 293 transfection kit according to the manufacturer’s protocol (ThermoFisher). Antibody containing cell culture supernatants were harvested after 5 days of cultivation in shake flasks ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: pcDNA4TO-TS-SNAP-β2AR or pcDNA4TO-TS-SNAP-β2AR-nLuc were stably transfected into T-RexTM-293 cells (Invitrogen) using polyethylenimine (PEI) ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI- cells (ATCC CRL-3022, lot number 62430067) were cultured at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... Protein expression was achieved by transient transfection of FreeStyle human embryonic kidney 293 (HEK293-F) cells (Thermo Fisher Scientific). Cells were grown in non-baffled shake flasks (Corning ...
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bioRxiv - Microbiology 2024Quote: ... Flp-In T-REx 293 cell lines with reconstituted PKR were maintained in 5% FBS DMEM supplemented with 50µg/mL hygromycin B (Invitrogen) and 15µg/mL blasticidin ...
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bioRxiv - Developmental Biology 2020Quote: ... goat anti-mouse Alexa 555 and 488 (Life Technologies, 1:400 and 1: 200 respectively).
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bioRxiv - Cell Biology 2019Quote: ... goat anti-mouse Alexa 594 and 488 (Life Technologies, 1:400 and 1: 200 respectively). The following antibodies were used for western blotting ...
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bioRxiv - Cancer Biology 2019Quote: ... then incubated in 1% BSA/PBS with 1:500 goat anti-mouse (Thermo Fisher, A21424) or goat anti-rabbit (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... or donkey anti-mouse IgG AF647 (for CC1) (1:200 or 1:250; Molecular Probes). All slides were counterstained with Hoechst dye to identify cell nuclei ...
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bioRxiv - Neuroscience 2021Quote: ... stained with rabbit polyclonal antibodies against mouse ZO-1 (1:100, Invitrogen, Carlsbad, CA, USA) and visualized with Alexa Fluor 594 (Invitrogen) ...
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bioRxiv - Bioengineering 2022Quote: ... mouse monoclonal zona occludens-1 Alexa Fluor 594-conjugated antibody (Thermo Fisher Scientific; 1:200), mouse monoclonal beta-catenin Alexa Fluor 647-conjugated antibody (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... The following antibodies and dilutions were used: mouse anti-ZO-1 (339100 Invitrogen, 1:200), anti-pMLC2 (3671 Cell Signaling ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 1 h at RT with fluorescently labelled goat anti-mouse (1:10000, Invitrogen) in 5% milk in PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-mouse monoclonal pS202/pT205 1:1000 (AT8, 1:500, #MN, 1020 from Thermo Fisher) was used ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and anti-mouse IgG (gamma 1) Alexa Fluor 488 (goat polyclonal, Invitrogen, AB_2535764, 1:2000) were used as secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse IgG1 anti-alpha Tubulin Clone B-5-1-2 (1:500, ThermoFisher 32-2500), Rabbit anti-Myc polyclonal (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... An AF555 conjugated mouse antibody was used to stain ZO-1 (1:100, Thermofisher Scientific) followed by incubation with a secondary-antibody to amplify the fluorescent signal ...
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bioRxiv - Neuroscience 2024Quote: Mouse-hosted antibodies [anti-rat endothelial cell antigen (RECA-1; 1:50, MA1-81510, Invitrogen), anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cell Biology 2024Quote: ... Mouse anti-ZO-1 Alexa Fluor 647 conjugated antibody (1:100, ThermoFisher MA3-39100-A647) diluted in blocking buffer containing Hoechst 33342 (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... BrdU-labelled cells were marked by immunohistochemistry (primary antibody: MoBU-1, mouse, 1:250, ThermoFisher #B35128 ...
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bioRxiv - Cancer Biology 2023Quote: ... and Alexa Fluor™ 594 donkey anti-mouse (1 µg mL-1; Invitrogen A-21203). Images were acquired on a Nikon 90i microscope equipped with a Plan Apo VC 60X/1.40 NA oil objective and controlled by MetaMorph® (Molecular Devices) ...
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bioRxiv - Neuroscience 2023Quote: ... and 1% bovine serum albumin) containing 1:400 goat anti-mouse Alexafluor 594 (Thermo Fisher) and 1:400 Goat anti-rabbit AlexaFluor 488 (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated for 1 hour with Alexa 488/568 secondary (1:1000; Life Technologies, mouse) and DAPI (1:5000 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... mouse-anti-V5 (1:500 for IHC and 1:1,000 for WB, ThermoFisher R960-25), rabbit-anti-Bassoon (1:500 ...
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bioRxiv - Pathology 2021Quote: ... The supernatant was moved to a clean tube and incubated overnight with 0.5 μg of either goat anti-mouse KIM-1 or mouse anti-MUC1 (B27.29) antibodies and Protein G conjugated to Sepharose 4B (ThermoFisher). Beads were washed with 0.5 ml HEPES-buffered saline (HBS ...
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bioRxiv - Pathology 2023Quote: ... and then with the appropriate secondary antibody (Alex Fluor 488 anti-mouse IgG2b, Alex Fluor 555 anti-mouse IgG2a, or Alex Fluor 647 anti-rabbit IgG; 1:1,000, Invitrogen). Slides were observed using an LSM 710 Zeiss confocal laser microscope ...
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bioRxiv - Genetics 2024Quote: ... followed by HRP-conjugated goat-anti mouse secondary antibodies at 1/20,000 dilution (Goat anti-mouse-HRP conjugate, cat# 31430, Thermofisher). The proteins were detected by SuperSignal chemiluminescent substrate (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... Standard Western blotting procedures were used with the following antibodies: HIV-1 gp120 (NIH AIDS Reagent program catalog no. 288) and 6x-His Tag (ThermoFisher catalog no. MA1-21315-HRP).
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bioRxiv - Developmental Biology 2021Quote: ... C2cd6s was subcloned into pcDNA3.1/myc-His A vector (Cat. No. V800-20, Invitrogen). The others were TA-cloned to pcDNA3.1/V5-His TOPO TA vectors (Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... using the Ion PI™ Hi-Q™ OT2 200 kit (Invitrogen; Cat #A26434) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% heat-inactivated fetal bovine serum [HI-FBS] (Cat#SH30071.03, Thermo Scientific), penicillin [100 IU/ml]-streptomycin [100 ug/ml] (Quality Biological ...
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bioRxiv - Molecular Biology 2019Quote: ... BAC DNA extraction was performed using Hi-Pure Plasmid DNA Extraction Kit (Invitrogen K210017). Nick translation of BAC DNA was performed using the Nick Translation kit (Roche 11 745 808 910) ...
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bioRxiv - Molecular Biology 2019Quote: ... full-length MmEsco2 was cloned into the pcDNA3.1/myc-His vector (Thermo Fisher Scientific). Subsequently ...
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bioRxiv - Immunology 2021Quote: ... The extracellular domain of NKp46 was cloned into pcDNA Myc-His 3.1a vector (Invitrogen) or pCMV vector (Addgene plasmid #59314 ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleared lysate was incubated with 1mL His-Pur nickel-NTA resin (Thermo Fisher) with rotation at 4 °C for 1 hour ...
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bioRxiv - Molecular Biology 2020Quote: ... ZAP-S and ZAP-L were subcloned into pEF1-V5/His (Thermo Fisher Scientific) via the KpnI/XbaI sites to generate pEF1-ZAP-S-V5/His and pEF1-ZAP-L-V5/His ...
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bioRxiv - Neuroscience 2022Quote: ... the pEx-FGD4-His-V5 vector was generated by Gateway cloning technology (Thermofisher, USA). Transfection experiments were performed using promofectin reagent (#PK-CT-2000-50 ...
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bioRxiv - Synthetic Biology 2022Quote: All antigens were His-tagged and purified using HisPur™ Ni-NTA resin (ThermoFisher). Cell supernatants were diluted with 1/3rd volume wash buffer [20 mM imidazole ...
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bioRxiv - Immunology 2022Quote: ... unbiotinylated form were incubated with 2μg/mL anti-His biotin (Invitrogen, MA1-21315-BTIN) for 20 min at room temperature before being used to label the streptavidin beads ...
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Assessment of Human Renal Transporter Based Drug-Drug Interactions Using Proximal Tubule Kidney-ChipbioRxiv - Cell Biology 2022Quote: ... Heat inactivated fetal bovine serum (HI FBS) and trypan blue were procured from Gibco Life Technologies (Waltham ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 10% heat inactivated fetal bovine serum (HI-FBS, Life Technologies unless indicated), 100 U/mL penicillin-streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... 3 μg of mammalian expression vector plasmids based on pEF1α V5 His C (Invitrogen) encoding codon-optimized gH ...
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bioRxiv - Microbiology 2019Quote: Qst was fused to a His-tag using the pEXP5-CT/TOPO vector (Invitrogen) following the TA-cloning protocol provided by the manufacturer ...
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bioRxiv - Immunology 2019Quote: ... 1xPen/Strep/Glu and 10% ultralow IgG HI-FBS (Thermo Fisher Scientific, Waltham, MA), and pelleted at 350xg for 5min ...
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bioRxiv - Biochemistry 2019Quote: ... A full length cDNA of Matriptase constructed in pcDNA3.1-V5-His (Thermo Fisher Scientific) was provided by Dr ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged proteins were purified using HisPur Ni-NTA resin (Thermo Fisher Scientific), at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... Then the blot was incubated with HRP conjugated anti-His antibody (Invitrogen, LOT 1902132) at 1:5000 dilutions for 16h at 40 C ...
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bioRxiv - Immunology 2021Quote: ... and the protein was purified on His-Pur Ni-NTA resin (Thermo Fisher Scientific) followed by size-exclusion chromatography ...
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bioRxiv - Cancer Biology 2020Quote: ... HA-tagged RON was cloned into the expression vector pcDNA3.1/V5-His-TOPO (Invitrogen) by fusion PCR ...
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bioRxiv - Microbiology 2020Quote: ... 1U Platinum High Fidelity (Hi-Fi) proof reading Taq polymerase (Invitrogen, Carlsbad, CA, USA). Two colonies of each G ...