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Citations for Thermo Fisher :
3301 - 3350 of 10000+ citations for Recombinant Human PDCD1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (ACE2, dilution 1:100; TMPRSS2, 1:500; CD147, 1:500; 6x-HIS-tag (Invitrogen MA1-21315), 1:1000 ...
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bioRxiv - Genetics 2020Quote: ... Purified N-HIS-FAM19A5 was then digested overnight at 30°C with AcTEV protease (Thermo Fisher Scientific, USA) to remove the His-tag ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were next treated with 1: 1000 dilution of primary antibody (6-His tag mouse anti-tag, Invitrogen) for 1 hr at RT ...
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bioRxiv - Immunology 2022Quote: ... Wildtype and mutant coding sequences were transferred to the pEF1/V5-His vector (cat. no. V920; Life Technologies) for overexpression ...
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bioRxiv - Synthetic Biology 2022Quote: ... Anti-His immunoblots were performed using 1:2,500 diluted rabbit Anti-6xHis antibody (PA1-983B, Thermo Fisher Scientific) in 5% w/v milk in TBST for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... we followed the vendor supplied protocol of the His-Pur Ni-NTA Purification Kit (3 ml, Thermo Fisher). The flow through ...
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bioRxiv - Molecular Biology 2024Quote: ... The washed membrane was then incubated overnight at 5°C with a 6x-His-Tag monoclonal antibody (Invitrogen) diluted 2500-fold in PBS-Tween ...
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bioRxiv - Immunology 2023Quote: HEK293 cells were transfected with pcTBK1-His or pcGSK3B-Flag plasmid using Lipofectamine 2000 (Invitrogen, Waltham, MA, US) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription for other transcripts was performed using the hi-capacity cDNA reverse transcription kit (Applied Biosystems #4368814) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... We diluted anti-stem Abs (starting at the lowest concentration, which yielded measurable HI) in C1q-DPBS (Gibco) (220 nM) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with a pMT Puro His-PC-SNAP-Elp3 vector using ExpiFectamine™ Sf(A389l5, Gibco) using manufacturer’s instruction ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:1000 dilution of chicken anti-His antibody (Invitrogen, PA1-9531) on ice for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 10% Hi-FBS (Thermo Fisher Scientific). BSC-1 cells (ATTC ...
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bioRxiv - Microbiology 2023Quote: ... TCID50) were calculated by IFA using HIS α NDV and Alexa FluorTM 488 α-rabbit secondary antibody (Invitrogen) in two independent experiments.
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bioRxiv - Microbiology 2024Quote: The HylB expression vector pAOJ66 was generated by amplifying the ef0818 gene with primers AOJ_651 and AOJ_652 and cloning into NcoI-HF/ApaI digested pBAD/myc his A (Invitrogen). The vector was propagated in E ...
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bioRxiv - Developmental Biology 2024Quote: Drosophila S2 cells were transfected with the indicated plasmids or blank pMT-V5-His vector (Invitrogen cat#V412020) using Effectene transfection reagent (Qiagen cat#301427) ...
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bioRxiv - Genetics 2024Quote: ... and hybridizing the Hi-C libraries with custom probes before enrichment via Dynabeads MyOne Streptavidin Beads T1 (ThermoFisher). After library enrichment ...
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bioRxiv - Immunology 2024Quote: ... Blots were blocked for 30 min to 1 h in 5% milk in TBST (1X Tris-buffered saline with 0.1% Tween 20) and then incubated with an anti-6X-His tag primary antibody (1:1,000 dilution, Invitrogen) (overnight ...
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bioRxiv - Plant Biology 2024Quote: Cloning and expression of His-mVenus-SUF4 was based on the Bac-to-Bac Baculovirus Expression system (ThermoFisher). The control pFastBac-mVenus and pFastBac-mVenus-SUF4 isoform 1 plasmids were obtained from Dr ...
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bioRxiv - Cell Biology 2022Quote: ... cells were co-stained with recombinant Cholera toxin subunit B conjugated to Fluor488 (Invitrogen C34775) at 1 μg/mL for less than 1 minute prior to fixing and staining for NINJ1 as described above ...
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bioRxiv - Physiology 2019Quote: Recombinant adenoviruses were generated using the pAd/CMV/V5-DEST Gateway recombination system (Life Technologies) after cloning the full-length cDNA into the pENTR vector ...
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bioRxiv - Biochemistry 2019Quote: ... The resulting antiserum was affinity purified using recombinant CTLMA2 immobilized on Aminolink resin (Thermo Fisher). Western blotting was performed with affinity purified anti-CTLMA2 ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant BACs were isolated from the bacterial hosts and used to transfect Sf9 cells (Invitrogen).
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bioRxiv - Biochemistry 2021Quote: ... The recombinant IgG (XBA14 and XBA18) was produced in FreeStyle 293 cells (Thermo Fisher Scientific), and purified by Protein A-Sepharose (Cytiva ...
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bioRxiv - Bioengineering 2019Quote: ... and genomic DNA was digested using Optizyme™ recombinant DNase-I digestion kit (Thermo Fisher) per manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... The expression of recombinant IL-18 was induced by 0.5 mM IPTG (Thermo-Fisher Scientific) for 8 h at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with recombinant EGF and bovine pituitary extract according to the manufacturer’s instructions (Thermo Fisher), as well as 100 μg/ml Primocin (Invivogen) ...
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bioRxiv - Microbiology 2020Quote: ... BMDCs were differentiated with recombinant murine granulocyte-macrophage colony-stimulating factor (20 ng/ml; Invitrogen), while BMDMs with macrophage colony-stimulating factor (10ng/ml ...
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bioRxiv - Biophysics 2021Quote: ... and 6xHis tags Recombinant baculoviruses were generated using the Bac-to-Bac system (Thermo Fisher). High Five cells (Thermo Fisher B85502 ...
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bioRxiv - Microbiology 2019Quote: ... Samples were shifted to RT and treated with 1 µl RNaseOUT Recombinant Ribonuclease Inhibitor (ThermoFisher) and 0.5 µl Thermo Scientific Maxima H Minus Reverse Transcriptase ...
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bioRxiv - Plant Biology 2021Quote: ... Gateway recombinant cloning was performed to subclone the aforementioned constructs into pDEST™24 (Invitrogen) using a LR cloning reaction (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... The recombinant plasmids were isolated by using Gene JET plasmid miniprep kit (Thermo Scientific, USA). The recombinant plasmids of SCD and SREBP1 genes were transfected into the CEH by using the Gene Pulser XcellTM Electroporation system (Biorad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant baculoviruses expressing A2AAR WT or mutants were prepared using Bac-to-Bac system (Invitrogen). Spodoptera frugiperda 9 (Sf9 ...
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bioRxiv - Neuroscience 2021Quote: ... 1 ng Cholera Toxin subunit B (CT-B) (Recombinant Alexa Fluor 488 conjugate, Life Technologies) was injected into the hindbrain ventricle81 ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant lentivirus was produced by transient transfection of 293T cells using Virapower packaging mix (Invitrogen). Cells were plated into 2 well LabTek chambered coverglass (Nunc ...
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bioRxiv - Cell Biology 2019Quote: ... Transposition and the generation of recombinant baculovirus were performed following manufacturer’s protocols (Thermo Fisher Scientific). For protein production ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant CP1Ag was expressed and initially purified via a nickel-affinity column (HisPur Thermo Fisher) as previously described [64] ...
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bioRxiv - Physiology 2023Quote: Balb/c mice were injected subcutaneously (s.c.) with 40IU of EPO (Recombinant HuEpo-alpha, Gibco) for four consecutive days ...
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bioRxiv - Physiology 2024Quote: ... according to the manufacture protocol and treated with recombinant Rnase-free DNAse I (Thermo Scientific). RNA concentration and purity were determined with NanoDrop 2000 Spectrophotometer (ND-2000 ...
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bioRxiv - Physiology 2024Quote: ... The Taq DNA Polymerase recombinant PCR kit (Cat no. 10342-020, Thermo Fisher Scientific, USA) was used following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... in presence of 40 units of RNaseOUTTM Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific #10777-019) and 200 units of Reverse Transcriptase per 20 µL of reaction mixture ...
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bioRxiv - Cell Biology 2023Quote: ... the recombinant adenovirus extract was injected into Slide-A-Lyzer Dialysis Cassettes (Thermo Fisher Scientific) and dialyzed in a dialysis buffer at 4 °C with stirring ...
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bioRxiv - Genomics 2024Quote: ... 50 µL of 20 mg/mL recombinant Proteinase K solution (Life Technologies, Catalogue number: AM2548) was then added ...
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bioRxiv - Microbiology 2024Quote: ... equi were prepared using a recombinant plasmid from the TA cloning® kit (Invitrogen, USA). A 10-fold serial dilution of the plasmid (from an initial concentration of 0.5x108 copy number/μL ...
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bioRxiv - Biochemistry 2024Quote: Recombinant bacmid production was carried out under manufacturer’s instructions (Bac-to-bac system, Life Technologies). Competent E ...
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bioRxiv - Biophysics 2023Quote: ... and subsequently incubated in the same buffer with 1:300 Tom20 Recombinant rabbit antibody (Invitrogen) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant baculoviruses were recovered after transfection of High Five cells using Cellfectin reagent (ThermoFisher Scientific) and amplification.
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bioRxiv - Molecular Biology 2023Quote: ... recombinant GR-LBD-GST (A15668) and Nuclear Receptor Buffer F (PV4547) were all from Invitrogen.