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Citations for Thermo Fisher :
3301 - 3350 of 6821 citations for Recombinant Human EFNA3 None tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... CRC human organoids were cultured in organoid media (advanced DMEM/F12 (Gibco) supplemented with EGF (R&D Systems) ...
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bioRxiv - Immunology 2023Quote: ... were coated with either goat anti-human Ig (10 μg/ml, Invitrogen) or recombinant SARS-CoV-2 Hu-1 −RBD or −S or BA.1-RBD or −S protein (2.5 μg/ml each ...
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bioRxiv - Cell Biology 2023Quote: ... antibody and goat anti-human Alexa-Fluor 633 (Life Technologies, 1:200). To visualize Aurora kinase C ...
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bioRxiv - Immunology 2023Quote: ... Human CD3/CD28 T-cell expansion Dynabeads were purchased from Thermo Fisher Scientific.
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were probed with donkey-anti-human Alexa Fluor 488 (Invitrogen) for 2 hours at room temperature and mounted in Prolong Gold (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... washed and detected with HRP conjugated anti-human IgG (Thermo Fisher, #A18811) or anti-mouse IgG (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... Alexa Fluor 488-conjugated goat anti-human IgG secondary antibody (Invitrogen, #A11013) diluted 1:500 was used to detect human primary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Alexa Fluor 488-conjugated goat anti-human IgG secondary antibody (Invitrogen, #A11001) diluted 1:500 was used to detect human primary antibodies ...
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bioRxiv - Systems Biology 2023Quote: Different human cell lines (HeLa, HEK293) were cultured in DMEM (Gibco, Invitrogen), supplemented with 10% fetal bovine serum ...
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bioRxiv - Cancer Biology 2023Quote: ... and Dynabeads human T-activator CD3/CD28 (aCD3/CD28; #11131D, Gibco, USA) in a 1:1 cell-bead ratio38 ...
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bioRxiv - Cancer Biology 2023Quote: ... rabbit anti-human phosphor-CJUN antibody (1:2500, PA5-40193, Invitrogen, USA), rabbit anti-human CJUN antibody (1:2500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody against human/mouse ABCA1 (#PA1-16789; RRID:AB_2288917) was purchased from Invitrogen Life Technologies (Carlsbad ...
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bioRxiv - Bioengineering 2023Quote: Primary human T cells were cultured in serum-free media (Thermo Fisher) containing 100 IU/ml recombinant human IL-2 ...
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bioRxiv - Bioengineering 2023Quote: A human-codon optimized bPAC gene was synthesized (GeneArt, ThermoFisher, Waltham, MA) from the available bPAC sequence (accession number ...
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bioRxiv - Cell Biology 2023Quote: Human iPSCs were cultured in feeder-free condition with Stemflex (Gibco, A3349401) or mTeSR plus (Stemcell Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... or anti-human CD68 (IgG3, kappa, PG-M1, ThermoFisher Scientific, MA, USA) antibodies were diluted 1:50 in PBS supplemented with 0.1% BSA and applied on the devices overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... A549 human lung epithelial cells (ATCC) were cultured in F12K media (Gibco) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... SW13 human adrenocortical carcinoma cells were cultured in high glucose DMEM (Invitrogen) supplemented with 10% fetal calf serum and antibiotics ...
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bioRxiv - Cell Biology 2023Quote: ... and/or Alexa Fluor 647-conjugated human transferrin (25 μg/ml; Invitrogen) were added to the cells for some experiments.
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... goat anti-human IgG (H+L) Alexa Fluor™ 647 (Thermo Fisher Scientific Cat# A-21445 ...
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bioRxiv - Biophysics 2023Quote: ... incubated with horseradish peroxidase-conjugated anti-human IgG (InvitrogenTM, ThermoFisher, Waltham, MA) and anti-mouse IgG (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... Leptin levels were determined using Human Leptin Instant ELISA kit (Invitrogen BMS2039INST) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... and Alexa Fluor594-conjugate human transferrin (T13343) were obtained from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... PE-coupled mouse anti-Human CD86 (Invitrogen, 12-0869-42; 1/100), APC-coupled mouse anti-Human CD83 (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2024Quote: Human iPSCs from the WTC11 background were cultured in StemFlex Medium (GIBCO/Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... or human siCIC (Horizon L-015185-01-0020) using Lipofectamine RNAiMAX (Invitrogen). Differentiation media was then changed two and four days after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... and mouse anti-human CD45RO PE-Cy7 (Invitrogen Cat 25-0457-42). Flow cytometric data were analyzed using SpectroFlo (Cytek ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 5 ng/mL of human epidermal growth factor (hEGF, ThermoFisher), 20 ng/mL of nerve growth factor (NGF ...
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bioRxiv - Cell Biology 2024Quote: ... the medium was further changed to Human Endothelial SFM (Thermo Fisher Scientific) supplemented with 5% Knockout serum replacement (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... goat anti-human H+L conjugated Alexa 488 (Thermo Fisher Scientific A11013) as the secondary antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... incubated with a rabbit anti-human CRIP1 primary antibody (ThermoFisher, #PA5-24643). Cells were then incubated with an Alexa-647 anti-rabbit secondary antibody and washed with flow buffer ...
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bioRxiv - Immunology 2024Quote: ... human iPSCs were induced to undergo definitive endoderm differentiation using RMPI1640 (Gibco) supplemented with Activin A (100 ng/ml ...
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bioRxiv - Genomics 2024Quote: ... Human GPR108 (NCBI RefSeq NP_001073921) was synthesized as GeneArt Strings by Invitrogen and cloned into pSBbi using Gibson cloning.
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bioRxiv - Developmental Biology 2024Quote: ... human or murine aortic SMCs were transfected with Lipofectamine 2000 (Life Technologies) containing Scr RNA or siRNA targeting ELN ...
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bioRxiv - Systems Biology 2024Quote: ... mouse anti-human Tau monoclonal antibody-HT7(1:2000; Thermo Fisher # MN1000); mouse anti-S396/S404 phospho-Tau monoclonal antibody-PHF1 (1:500 ...
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bioRxiv - Molecular Biology 2019Quote: ... and pol μ antibodies were affinity purified from rabbit serum by coupling each full-length recombinant protein to AminoLink Coupling Resin (Thermo Scientific) and following manufacturer’s instructions for IgG purification.
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bioRxiv - Biochemistry 2021Quote: Recombinant baculovirus carrying hXkr8-TH-mGFP or hBSGΔ cDNA was obtained with the Bac-to-Bac Baculovirus Expression System (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Recombinant biotinylated proteins (sE, sE-cvD and DIII) were expressed at 28°C using ExpiFectamine™ 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Genomics 2021Quote: Cryopreserved stocks of the KH004 parent and the Mal31×KH004 uncloned bulk recombinant progeny pool and were thawed and grown in complete media (CM) [RPMI 1640 with L-glutamine (Gibco, Life Technologies), 50 mg/L hypoxanthine (Calbiochem ...
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bioRxiv - Biochemistry 2022Quote: ... Rhodopsin expressed at the plasma membrane of recombinant cells was labeled with a DyLight 550–conjugated anti-HA antibody (ThermoFisher, Waltham, MA). Labelled cells were then fractionated into quartiles according to surface immunostaining intensity using a FACS Aria IIu fluorescence activated cell sorter (BD Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... purified CD4+ T cells were maintained for two days in complete RPMI 1640 medium containing recombinant interleukin-2 (IL-2) (300 IU/ml) and phytohemagglutinin (1 μg/ml, Thermo Fisher Scientific), followed by 3 days in the presence of IL-2 alone ...
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bioRxiv - Bioengineering 2021Quote: ... Qiagen miniprep Kit`for recombinant plasmid isolation and purification and SDS-NuPAGE precast gels for molecular characterization of expressed enzyme were obtained from Invitrogen (Carlsbad, CA). Polygalacturonic acid (PGA ...
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bioRxiv - Microbiology 2019Quote: ... 125ng of total recombinant protein was separated on 4-20% or 10-20% Novex WedgeWell Tris-Glycine precast gels (Thermofisher, Waltham, MA). For silver stain ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant bacmids were selected by plating on Luria-Bertani (LB) agar plates with 50 μg/ml kanamycin (Gibco from Thermo Fisher Scientific) and 10 μg/ml tetracycline (MilliporeSigma ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant bacmids were selected by plating on Luria-Bertani (LB) agar plates with 50 μg/ml kanamycin (Gibco from Thermo Fisher Scientific) and 10 μg/ml tetracycline (MilliporeSigma ...
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bioRxiv - Microbiology 2021Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained with a polyclonal antibody raised in rabbit against purified recombinant BT1636 (BT1636Ab, Cocalico Biologicals) and detected with an Alexa Fluor® 488-conjugated goat anti-rabbit IgG secondary antibody (Molecular Probes). Images were taken with Zeiss Apotome using the same exposure time between samples.
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bioRxiv - Cell Biology 2020Quote: ... specimens were incubated with 10 nM purified recombinant POLArISact protein or 2-5 nM Alexa Fluor 488 Phalloidin (AF488-phalloidin, A12379, Thermo Fisher Scientific) and 10 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Immunology 2019Quote: ... In some groups culture media was supplemented with recombinant osteopontin (2 μg/ml) (R&D) or anti-CD44 (5 μg/ml) (Thermofisher; clone IM7). Cells were cultured in 5% CO2 at 37°C ...
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bioRxiv - Immunology 2020Quote: His-tagged recombinant mouse CD47 (as well as proteins and antibodies noted later) was labeled using AlexaFluor488 NHS Ester (Succinimidyl Ester, Thermo Fisher Scientific) reconstituted in anhydrous DMSO (dimethylsulfoxide ...