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Citations for Thermo Fisher :
3301 - 3350 of 5159 citations for Placental lactogen Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were washed with PBS and incubated in blocking buffer for 1 h with Streptavidin-AlexaFluor 488 (S11223, Molecular Probes), goat anti-Mouse IgG-AlexaFluor 555 (A-21424) ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting cell pellet was suspended in a blocking solution consisting of FACS buffer (1X PBS with 5% fetal bovine serum (Gibco) and 0.1% sodium azide ...
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bioRxiv - Cell Biology 2023Quote: ... ALFA-FAM134s were targeted directly using an anti-ALFA nanobody carrying a 5xR1 docking strand (5-TCCTCCTCCTCCTCCTCCT-3’) (Massive-TAG-Q Anti-ALFA Custom, Massive Photonics, GmbH) which was diluted in permeabilization/blocking buffer (10% FCS (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies rabbit anti-HA (1:500) was subsequently added in permeabilization/blocking buffer (10% fetal calf serum (FCS) (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... samples were permeabilized and blocked in blocking buffer (PBS+0.1% Triton-X100 plus 3% BSA and the Image-IT reagent (Thermo Scientific)) ...
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bioRxiv - Biochemistry 2024Quote: ... cells were permeabilized and blocked in 0.22 µm filter-sterilized 1X blocking buffer (10% normal goat serum (Thermo Scientific; 50197Z), 0.3% Triton X-100 ...
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bioRxiv - Bioengineering 2023Quote: ... the gels were washed with wash buffer three times for 5 min followed by permeabilization and blocking in 5% goat serum (Gibco), 1% BSA ...
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bioRxiv - Immunology 2024Quote: ... The wells were then preincubated with either 200µl of BSA blocking buffer (Thermo Scientific; 5% bovine serum albumin in PBS) for total binding (TB ...
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bioRxiv - Cancer Biology 2024Quote: ... Followed by two more PBS rinses prior to 1 hour incubation with SuperBlock™ Blocking Buffer (Thermo Scientific™,37515) for blocking non-specific antibody binding ...
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Deep brain stimulation induces white matter remodeling and functional changes to brain-wide networksbioRxiv - Neuroscience 2024Quote: ... these sections were incubated for 24 h with blocking solution (5% normal goat serum (Cat #PI31873, Invitrogen, Waltham, MA, USA) and diluted with PBS solution containing 0.3% Triton X-100 (TX-100) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed 1-2 times with 1X PBS and incubated for 2 hours in Alexa Fluor conjugated goat anti-rabbit/ chicken or donkey anti-goat/rabbit secondary antibodies along with DAPI (dilution 1:1000 X in blocking buffer) (Invitrogen) at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... followed by a 1-hour incubation in secondary antibody (diluted in blocking buffer, 1:1000, Alexa Fluor 488 donkey anti-goat (Invitrogen), 1:1000 Alexa Fluor 488 donkey anti-rabbit (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated 1 hour in blocking solution and incubated at 4 °C overnight with secondary antibodies conjugated with AlexaFluor (Molecular probes) diluted in blocking solution ...
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bioRxiv - Physiology 2024Quote: ... Cells were then immunostained using CDH5 antibody [36] diluted 1:100 in blocking buffer for 30 min followed by Alexa Fluor 555–conjugated goat anti–rabbit antibodies (ThermoFisher) diluted 1:200 in blocking buffer together with Hoechst ...
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bioRxiv - Immunology 2024Quote: ... for 1.5 hours (100 V constant, 4°C).The membrane was transferred into SuperBlock T20 (TBS) blocking buffer (Thermo Scientific) and blocked overnight while shaking at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... We then incubated membranes with secondary antibody (in 5% Blocking Buffer) for 1 hour at room temperature (Invitrogen cat. A16110; Invitrogen cat ...
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bioRxiv - Cell Biology 2024Quote: ... Microtubules were then stained using the rat anti-tubulin primary antibody (1:500 in blocking/permeabilization buffer, MA1-80017, Invitrogen) with a 4 °C overnight incubation ...
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bioRxiv - Neuroscience 2024Quote: ... and revealed with appropriate Alexa fluorophore-conjugated secondary antibodies (1:1000 in blocking buffer, A11013 and A21429, Thermofisher, Courtaboeuf, France) and DAPI (10 µg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... Then secondary antibodies were then added in blocking buffer over night at 4 ºC (Goat anti Rabbit 647, Thermo Fisher Scientific A21245 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were also labeled with phalloidin conjugated to Alexa Fluor 594 in blocking buffer according to manufacturer protocol (Thermofisher A30107). After incubation with DAPI for 5 min and subsequent PBS wash ...
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bioRxiv - Genomics 2024Quote: ... and pellets were resuspended in ice-cold bovine serum albumin (BSA) blocking solution (1× phosphate-buffered saline (PBS) (AM9625, Ambion), 0.5% BSA (VWR ...
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bioRxiv - Biophysics 2020Quote: ... 1 nmol of biotinylated 4-heptad CTD peptides (PeptideSynthetics) were incubated with 0.5 mg of Streptavidin-coupled magnetic Dynabeads M-280 (Invitrogen) in 300 µL of buffer A (25mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: Peptide fractions were analysed by nanoflow HPLC-MS/MS using an Ultimate 3000 nano RSLC system (Thermo Fisher Scientific) coupled with a Q-Exactive HF mass spectrometer fitted with a nano spray Flex ion source (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... the peptides were analyzed using nano flow reverse phase liquid chromatography on a Proxeon Easy-nLC 1000 (Thermo Scientific) coupled to an Orbitrap Elite (Thermo ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were separated using an analytical C18 Acclaim PepMap column (75 µm × 250 mm, 2 µm vesicles; Thermo Scientific) and a 180-min gradient at a flow rate of 300 µl/min ...
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bioRxiv - Cell Biology 2019Quote: Fractionated peptides were separated by nanoscale C18 reverse-phase liquid chromatography using an EASY-nLC II 1200 (Thermo Scientific) coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Peptide yield from each sample was determined to be approximately 20 µg based on BCA Protein Assay (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... was used to elute peptides from the capillary reverse-phase column (0.075 × 500 mm. Pepmap®. Thermo Fisher Scientific). Data were acquired using the Xcalibur 4.0 software ...
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bioRxiv - Cell Biology 2020Quote: ... The digested peptides were dried in a SpeedVac and labelled with TMT10plex as instructed by the manufacturer (Thermo Fisher). Labeled peptides were mixed ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Microbiology 2020Quote: ... The peptides were separated by C18 reversed phase liquid chromatography using an EASY-nLC 1000 system (Thermo Fisher Scientific). A set-up of pre-column and analytical column was used ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The resulting tryptic peptide mixtures were separated using Thermo EASY nLC 1200 series system (Thermo Fisher Scientific, MA, USA) coupled online with a Thermo Orbitrap Fusion™ Mass Spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: Peptides were measured using LC-MS instrumentation consisting of an Easy nanoflow HPLC system (Thermo Fisher Scientific, Bremen, Germany) coupled via a nanoelectrospray ion source (Thermo Fischer Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... LC-MS/MS analysis of the resulting peptides was conducted on a Q Exactive mass spectrometer (Thermo Fisher Scientific) coupled with an Easy-nLC1000 liquid chromatography system (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... 5µl of each peptide extracts were loaded onto 300 µm ID x 5 mm PepMap C18 precolumn (ThermoFisher, Dionex) at 20 µl/min in 2% acetonitrile ...
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bioRxiv - Cell Biology 2022Quote: For peptide analysis raw data processing and database searches were performed with Proteome Discoverer software 2.1.1.21 (Thermo Fisher Scientific). Peptide identifications were done with an in-house Mascot server version 2.5.1 (Matrix Science Ltd ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: Peptide fractions were analyzed by online nanoflow LC-MS/MS using a Proxeon easy-nLC 1200 system (Thermo Scientific) connected to a Q Exactive HF-X mass spectrometer (Thermo Scientific) ...
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bioRxiv - Biochemistry 2019Quote: ... Peptides were desalted (Acclaim PepMap 100, 75µm × 2 cm, nano viper, C18, 3 µm, 100 Å, ThermoFisher, Waltham, MA) and separated (Acclaim ...
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bioRxiv - Cell Biology 2019Quote: ... The tryptic peptides (∼1 µg loaded) were separated on a 50 cm × 75 µm (i.d.) column (Thermo Fisher Scientific) using a 120 min gradient of 12–45% acetonitrile ...
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bioRxiv - Microbiology 2019Quote: TMT-labelled tryptic peptides were subjected to HpRP fractionation using an Ultimate 3000 RSLC UHPLC system (Thermo Fisher Scientific) equipped with a 2.1 mm internal diameter (ID ...
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bioRxiv - Genetics 2019Quote: ... The identified proteins were filtered using the high peptide confidence setting in Protein Discoverer (version 2.2, Thermo Fisher Scientific), which correspond to a filter for 1% FDR on peptide level ...
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bioRxiv - Biochemistry 2019Quote: ... ATS-bound peptides were washed with 50 mM ammonium bicarbonate (3×) and eluted with 1 mM TCEP (Thermo Scientific) for 30 min at 37°C with constant rotation ...
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bioRxiv - Microbiology 2019Quote: ... The resulting peptides were subsequently labeled using a TMT-sixplex Isobaric Mass Tagging Kit (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... Peptides were trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... LC-MS/MS peptide analysis was performed on a linear trap quadrupole (LTQ) Orbitrap XL mass spectrometer (Thermo Fisher) and coupled to an Ultimate 3000 RSLC Nano UHPLC System (Dionex) ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting tryptic peptides were analysed by LC-MSMS using a Q Exactive coupled to an RSLCnano3000 (ThermoFisher Scientific). Raw files were processed using MaxQuant 1.5.2.8 using Andromeda to search a human Uniprot database (downloaded 03/12/14) ...
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bioRxiv - Immunology 2019Quote: ... Digested peptides were analysed by LC-MS/MS over a 30 min gradient using LTQ XL-Orbitrap (Thermo Scientific) at the Central Proteomics Facility (http://www.proteomics.ox.ac.uk ...
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bioRxiv - Cancer Biology 2020Quote: Desalted peptides from each sample (500μg) were labeled with 10-plex Tandem Mass Tag (TMT) reagents (ThermoFisher Scientific, 90110) (58) ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 µg of total peptides per condition were labeled with tandem mass tag (TMT10plex and TMT11, Thermo Fisher Scientific). After >98% TMT incorporation rate was confirmed ...
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bioRxiv - Biochemistry 2021Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...