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Citations for Thermo Fisher :
3301 - 3350 of 10000+ citations for Mouse IL 17D ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: One million cells PBMCs were incubated in 24 well plates with 30 IU/mL IL-2 (NCI) and with or without 25 μL of Human T-Activator CD3/CD28 dynabeads (ThermoFisher). Following 48 hours ...
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bioRxiv - Bioengineering 2022Quote: ... resuspended in assay medium (RPMI with 10% FBS and 20 IU/mL IL-2), and centrifuged (300xg, 5 mins) (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Skeletal muscle were stained with Myosin 4 monoclonal antibody (MF20, MyHC-2) (1:200; Thermo Fisher Scientific, Rockford, IL, USA) and Laminin gamma 1 Antibody (A5 ...
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bioRxiv - Plant Biology 2022Quote: ... and dialyzed overnight against water using 3.5 kDa molecular weight cut off Snake Skin dialysis tubing (Thermo Scientific, IL, USA). Dialyzed filtrate was loaded onto a HiPrep SP XL 16/10 cation exchange column (GE Healthcare Piscataway ...
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bioRxiv - Neuroscience 2022Quote: CeA punches were homogenized and assessed for protein concentration using a Pierce Bicinchoninic Acid (BCA) assay (Thermo Scientific, Rockford, IL) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: PER2::LUC rhythms were monitored for 42 hours prior to the addition of 10 ng/mL of IL-1α (R&D) or matching vehicle (UltraPure DNase/RNase-Free Distilled Water, Invitrogen). Explants were heat shocked immediately or three hours after IL-1α addition.
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bioRxiv - Bioengineering 2024Quote: ... the fluorescence intensity of the stained IFNγ and IL-2 antibodies was quantified using an Attune NxT Flow Cytometer equipped with an autosampler (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Complete M-PER buffer was prepared through addition of Protease & Phosphatase Inhibitor Cocktail (1X final) (Thermo Scientific, Rockford, IL, USA) to the M-PER buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 20 ng/mL TPO and 40 ng/mL IL-3 from day 1 to day 6 with a media refresh every 2 days (all cytokines from Thermofisher). On day 8 ...
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bioRxiv - Neuroscience 2023Quote: ... Lateral striatum was dissected from each hemi-section and placed in HABG media (Hibernate A Media, BrainBits, Springfield, IL, HA500; 2% B27 Supplement, ThermoFisher, Cat #17504044 ...
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bioRxiv - Immunology 2023Quote: ... Primary T lymphocytes stimulated with PHA and IL-2 (2 × 105, 100 µl/well) were resuspended in HBSS buffer (Gibco) containing 0.1% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Amplification of the cDNA samples was carried out using PowerUpTM SYBRTM Green Mastermix (Thermo Fisher Scientific-A25742, Rockford, IL, USA). The real-time PCR reactions were performed using the QuantStudio™ 3 Real-Time PCR System (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were washed a further two times in wash buffer before incubation with IL-6-PE (12-7069-82, Invitrogen) and IL-8 FITC (BH0814 ...
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bioRxiv - Immunology 2023Quote: ... penicillin/streptomycin (10 μg/mL) and supplemented with the cytokines IL-4 (500 U/mL) and GMCSF (800 U/mL) (both Gibco) [42] ...
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bioRxiv - Microbiology 2023Quote: ... at the Vienna Research Platform for Metabolomics & Proteomics and analyzed using the Proteome Discoverer v2.2.0.388 (ThermoFisher Scientific, Rockford, IL, USA) at the Life Science Computer Cluster (LiSC ...
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bioRxiv - Cell Biology 2023Quote: ... The LC-MS/MS analysis was performed using an LTQ Orbitrap Elite mass spectrometer (Thermo Scientific, Rockford, IL Waltham, MA) coupled online to an Easy-nLC 1000 in the data-dependent mode ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were obtained using RIPA buffer with the Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher, Rockford, IL, USA). The conditioned media (1.8 ml ...
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bioRxiv - Immunology 2023Quote: ... and complementary DNA (cDNA) was prepared by using Maxima™ H Minus cDNA Synthesis Master Mix (Thermo Scientific, Rockford, IL). Real-time qPCR (qRT-PCR ...
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bioRxiv - Immunology 2023Quote: 5×105 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were treated with 20ng/mL IL-4 for 5 hours before cells were detached using PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were stimulated with IL-4 (20ng/mL) for 24 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were stimulated with IL-4 (20ng/mL) for 24 hours before lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: 1 × 106 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 or PBS for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Immunology 2022Quote: IFNγ mRNA and protein were measured in IL-2 expanded MAIT cells (with or without stimulation with CD3/CD28 Dynabeads (Gibco)) and their culture supernatant by qPCR or ELISA respectively ...
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bioRxiv - Immunology 2022Quote: ... and activated in complete RPMI supplemented with 30 U/mL IL-2 with anti-human CD3/CD28 dynabeads (11131D, ThermoFisher) at a 1:1 cell-to-bead ratio ...
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bioRxiv - Immunology 2023Quote: ... using permeabilization buffer (eBioscience) and stained with the following cytokine antibodies for 30 min at 4°C: IL-17A (eBio17B7, FITC, Invitrogen), IFN-ψ (XMG1.2 ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were digested into peptides by adding 0.95 µg of MS grade trypsin (Thermo Scientific Pierce, Rockford, IL, USA) solubilized in 40 µl of ABC buffer to the filters and incubating for 16 hours in a wet chamber at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Gene expression levels were measured by quantitative real-time PCR using the SYBR Green assay (Thermo Scientific, Rockford, IL, USA)on a StepOne instrument (Thermo Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... in a final volume of 10□µl using an ABI Prism 7000 Sequence Detection System (Thermo Scientific, Rockford, IL, USA). The PCR reactions were initiated with a 10-min incubation at 95□°C followed by 40 cycles of 95□°C for 15□s and 60□°C for 60□s ...
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bioRxiv - Immunology 2024Quote: ... Untreated 96 flat bottom plates were coated overnight at 4°C with capture antibody for IFNγ or IL-17A from Invitrogen kits (Cat# 88-7314-22 and Cat# 88-7371-88 respectively from Invitrogen ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The membranes were then treated with secondary antibodies (biotinylated goat anti-rabbit IgG (#65-6140, Invitrogen, Rockford, IL, United States), biotinylated goat anti-rat IgG (#405428 ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of the supernatant for immunoprecipitation was measured via the bicinchoninic acid assay (Thermo Scientific, Rockford, IL, USA) (Walker ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse IgGs were stained with Alexa Fluor 568 conjugated donkey anti-mouse IgG (Invitrogen, Cat#A-10037). Panleucocytes were identified using rat anti-mouse CD45 antibodies (BD Pharmingen Inc ...
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bioRxiv - Neuroscience 2021Quote: ... biotinylated horse anti-mouse IgG (H+L) and biotinylated goat anti-mouse IgG2a (Life Technologies, ThermoFisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... biotinylated horse anti-mouse IgG (H+L) and biotinylated goat anti-mouse IgG2a (Life Technologies, ThermoFisher Scientific).
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bioRxiv - Bioengineering 2022Quote: ... Mouse IgG2a Fc Tag (Acro Biosystems) incubation followed with APC Goat anti-mouse IgG2a Fc Antibody (Invitrogen) staining and RFP expression ...
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bioRxiv - Molecular Biology 2020Quote: ... and mouse antibodies were coupled to Dynabeads coated with Pan Mouse IgG antibodies (Thermo Fisher Scientific, 11042). 50 μL of the appropriate Dynabeads pre-coupled with the indicated antibody were added to the chromatin sample and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... goat anti-mouse Alexa Fluor 568 or goat anti-mouse Alexa Fluor 488 secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Mouse monoclonal antibodies were detected with a goat anti-mouse IgG HRP-linked antibody (62-6520; Invitrogen) diluted 1:10,000 (v/v) ...
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bioRxiv - Immunology 2020Quote: ... Isotype control and mouse monoclonal antibody to mouse IFNβ (Clone: MAR1-5A3) were purchased from Thermo scientific. SP600125 and C-16 purchased from Calbiochem ...
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bioRxiv - Microbiology 2022Quote: ... CA) or Alexa Fluor 488 anti-mouse antibody (for LP11 and mouse anti-myc; Invitrogen, Waltham, MA) at a 1:250 dilution in FACS medium ...
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bioRxiv - Neuroscience 2024Quote: ... BV-2 cells were fixed and sequentially incubated with Mouse-on-Mouse IgG Blocking Solution (Invitrogen R37621) and Navenci blocking solution at 37°C for 60 min each in a pre-heated humidified chamber ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor 488 goat anti-mouse and anti-rabbit IgG (Invitrogen, Waltham, MA; catalog # A-11001 (mouse) and A-11008 (rabbit) ...
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bioRxiv - Neuroscience 2024Quote: ... and 647 (1:500 for all; PIA32773-mouse; A21202-mouse; PIA32731TR-rabbit; PIA32794-rabbit; A21448-sheep, Invitrogen). The sections were examined using an AX-R Confocal Microscope coupled to a fully automated Ti2-E system (Nikon Instruments Inc ...
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bioRxiv - Microbiology 2024Quote: ... and amastigotes were labeled with mouse anti-P8 and A488-conjugated goat anti-mouse secondary antibody (Invitrogen); samples were co-stained with DAPI ...
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bioRxiv - Microbiology 2024Quote: ... and amastigotes were relabeled with mouse anti-P8 and A488-conjugated goat anti-mouse secondary antibody (Invitrogen); samples were co-stained with DAPI ...
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bioRxiv - Physiology 2021Quote: Skeletal muscle was isolated from left thigh of the mouse and mitochondria were isolated from using mitochondria isolation kit (Thermo Scientific TM, Catalog no. 89801) using manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The diluted protein was coated in an increasing gradient (25-200 ng well-1) on a 96-microtiter ELISA plate (Nunc, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Immunology 2019Quote: ... The reaction was stopped with 3 N H2SO4 and read at 492 nm on a MultiSkan GO ELISA plate reader (Thermo Fisher Scientific, Vantaa).