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Citations for Thermo Fisher :
3301 - 3350 of 10000+ citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The staining was visualized by fluorescence microscopy with fluorescent-conjugated secondary antibodies (goat anti rabbit Alexa Fluor 594, 1:400, ThermoFisher,Waltham, MA, cat. A-11034). Nuclei were counterstained with Hoechst 33348 dye (1:1000) ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells (1.5×105 cells/mL) were labeled with a fluorescent dye 2’,7’-bis(carboxyethyl)-5 (6)-carboxyfluorescein-AM (Thermo Fisher Scientific B1150; 1 mg/mL) in serum-free RPMI medium (Thermo Fisher Scientific 11875093 ...
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bioRxiv - Neuroscience 2020Quote: Each organotypic slice was incubated for 1 h prior to imaging with the fluorescent Ca2+-indicator dye Fluo-4-AM (Molecular Probes, Invitrogen, Carlsbad, CA, USA). The conditions for incubating the dye were standardized across different slices to avoid uneven dye loading ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue sections were washed with PBS for 10 minutes and then incubated at room temperature with fluorescent Nissl (NeuroTrace 640/660 Deep Red, ThermoFisher N21483, dilution of 1:100) in PBS for 1 hr to stain neuronal cell bodies ...
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bioRxiv - Neuroscience 2020Quote: ... The cultures were then washed three times with PBS and then incubated for two hours with the following fluorescent secondary antibodies: Alexa Fluor 568-conjugated donkey anti mouse (1:2000, Life Technologies-Invitrogen, Carlsbad, CA, USA), Alexa Fluor 488-conjugated donkey anti rabbit (1:2000 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated for 45min at room temperature with secondary antibodies conjugated with Alexa Fluor 594 fluorescent dyes (Molecular Probes, 1:400 dilution in PBS+). Next ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 1.5 hours at RT with the corresponding secondary fluorescent antibodies diluted 1:1000 in blocking solution: goat anti-chicken Alexa FluorTM 568 nm (Invitrogen ref. A-11041, RRID AB_2534098), goat anti-rabbit Alexa FluorTM 647 nm (Invitrogen ref ...
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bioRxiv - Neuroscience 2024Quote: ... followed by staining with Neurotrace 500/525 green (1:300 for two hours, NeuroTrace™ 500/525 Green Fluorescent Nissl Stain – Solution in DMSO, Thermo Fisher Scientific, MA USA). Following two PBS washes ...
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bioRxiv - Microbiology 2021Quote: ... the purified PCR products were normalized to equimolar concentration with the SequalPrep Normalization Plate Kit (Thermo Fisher Scientific) and the DNA library was sequenced on the Illumina MiSeq next generation sequencing system (Illumina Inc. ...
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Hypoxia-mediated suppression of pyruvate carboxylase drives tumor microenvironment immunosuppressionbioRxiv - Cancer Biology 2022Quote: ... Labeled cDNA was hybridized to the plate using GeneTitan Hybridization Wash and Stain Kit for WT Arrays (Affymetrix).
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bioRxiv - Molecular Biology 2022Quote: ... The same plate was used for evaluating SOD (Superoxide Dismutase Colorimetric Activity Kit – Thermo Fisher Scientific – ref EIASODC) and CAT (Catalase Colorimetric Activity Kit –Thermo Fisher Scientific – ref EIACATC ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were normalized with the SequalPrep Normalization Plate Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) and eluted in 20 μL elution buffer ...
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bioRxiv - Immunology 2023Quote: ... CpG content was measured in a 96 well plate using Ribogreen™ fluorescence staining kit from Thermo Fisher and a SpectraMax i3 plate reader (Molecular devices ...
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bioRxiv - Microbiology 2023Quote: ... Duplicate PCRs were combined prior to clean up and normalization with a SequalPrep Normalization Plate kit (ThermoFisher Scientific) following manufacturer instructions using 25 µL of PCR product per sample ...
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bioRxiv - Immunology 2023Quote: ... CpG content was measured in a 96 well plate using Ribogreen™ fluorescence staining kit from Thermo Fisher and a SpectraMax i3 plate reader (Molecular devices ...
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bioRxiv - Microbiology 2023Quote: ... triplicate first-step PCR reactions were pooled and purified and normalised with the SequalPrep Normalisation Plate Kit (Invitrogen) following the manufacturers’ instructions ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was quantified on a plate reader using the Pierce BCA protein assay kit (#23225, Thermo Scientific). Gel lanes were loaded with 30 µg of total protein ...
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bioRxiv - Immunology 2021Quote: ... The bottom chamber of the plate contained PBS with 20 ng/well monocyte chemoattractant protein-1 (MCP-1, Thermofisher Scientific, USA). Macrophages (5×104 cells in PBS ...
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bioRxiv - Immunology 2021Quote: ... 5×105 murine or human T cells have been stimulated at 1:1 ratio in 48well plate in presence of Sulfate Latex 4% w/v 5µm Beads (Molecular Probes S37227). For each experiment ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cells were washed four times with PBS (10min at RT for each wash) and then ECL (1:1) was added to detect the signal by the plate reader (Varioskan Flash, Thermo Scientific). The signal from the samples ...
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bioRxiv - Immunology 2020Quote: ... monocytes were plated in low-serum media (1% FBS) for 1-2 hours in 12-well Nunc-coated plates (ThermoFisher Scientific) to promote adherence ...
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bioRxiv - Evolutionary Biology 2022Quote: ... then mixed 1:1 and diluted 400-fold in 200 µl of fresh LB in 96-well plates (Thermo Scientific, Denmark), and incubated during 24 in the same conditions ...
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bioRxiv - Cancer Biology 2022Quote: Organoids were collected from culture plates and dissociated into single cells using a 1:1 mixture of TrypLE Express (Gibco, 12604013) & Accutase (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... and THP-1 cells (100 μl of 1 x 106 cells/mL) were seeded in 96 well tissue culture plate (Nunc™, Thermofisher) for 24 hours using DMEM and RPMI media respectively supplemented with 10% heat inactivated FBS in 5% CO2 incubator at 37°C ...
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bioRxiv - Immunology 2020Quote: ... were cultured for 16 h in each well of a 12 well plate in serum-free medium (MEM Glutamax, containing 1% v/v Penicillin-Streptomycin and 1%v/v sodium pyruvate [Gibco, Thermofisher]). SARS-CoV-2 clinical samples (100 TCID50/ well ...
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bioRxiv - Neuroscience 2023Quote: ... Rosettes were plated on growth factor reduced matrigel coated 6 well plates in ‘pNPC medium’ composed of a 1:1 mixture of Neurobasal (ThermoFisher Scientific) and DMEM/F12 ...
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bioRxiv - Neuroscience 2024Quote: ... coated organoids were returned to the 24-well ultra-low-attachment plates with differentiation media without vitamin A [1:1 mixture of DMEM/F12 and Neurobasal (Gibco, USA), 0.5% N2 supplement ...
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bioRxiv - Cancer Biology 2023Quote: ... the cell pellet was resuspended and cultivated in a 24-well plate with a 1:1 medium of defined Keratinocyte-SFM (Thermo Fisher) and complete embryonic fibroblast medium (Cell Biologics) ...
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bioRxiv - Cell Biology 2023Quote: ... Airyscan processed images were then registered to correct for chromatic shift from the microscope using either TetraSpeck™ Fluorescent Microspheres Size Kit (mounted on slide) (Thermo Fisher Scientific, T14792, 0.2 µm bead size) or TetraSpeck™ Microspheres ...
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bioRxiv - Microbiology 2021Quote: Ninety-six well flat bottom plates (Immulon 4 HBX plates, ThermoFisher Scientific) were coated with 50 μl/well of 2 μg/ml recombinant protein in 1x coating buffer (Seracare ...
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bioRxiv - Genetics 2019Quote: ... and the plates sealed using breathable plate seals (Thermo Scientific # AB-0718) and incubated overnight with vigorous shaking on a 2.5 mm radius orbital plate shaker overnight at 37°C ...
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bioRxiv - Systems Biology 2019Quote: ... The deep well plate was placed on a magnetic plate (Life technologies) and all but 300 µl of the supernatant removed and discarded ...
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bioRxiv - Microbiology 2019Quote: ... Robotic plate handling was used to load and unload plates (Thermo Scientific). The objective was a 10X S Fluor with 0.45NA ...
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bioRxiv - Cell Biology 2021Quote: ... the plate was sealed with adhesive PCR plate foil (Thermo Fisher Scientific) and kept in a dry and dark space ...
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bioRxiv - Microbiology 2022Quote: ... The wells of 96-well microtiter plates (Immulon 2HB plates, Thermo Scientific) were coated with 100 µL of each diluted aliquot in triplicate and incubated for 18-20 hours at 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... Well plates (Standard clear 96-well plate with 1mm polystyrene-base, ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The plates were sealed with an adhesive PCR plate seal (Thermo Scientific) and incubated for 24-hours at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... The plate was sealed with a plate sealer film (Nalgene Nunc International) and then incubated at 37 °C in a BMG FLUOstar Omega plate reader ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... aliquoted in 96-well V-bottom plates (V96 PP Plate, Thermo Fisher) and frozen at -20°C until the day of the treatment ...
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bioRxiv - Immunology 2024Quote: ... Plates were analyzed using a Multiskan FC plate reader (Thermo Fisher Scientific), and absorbance was measured at 450 nm and 630 nm ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Then 65 μL of 300 nM SYTOX green (Thermo Fisher Scientific, S-34860) was added to each well and the cultures were mixed and incubated at room temperature in the dark for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with human keratinocyte growth supplement (catalogue #S-001-5, Thermo Fisher Scientific) and 1% penicillin/streptomycin (catalogue #15140-122 ...
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bioRxiv - Molecular Biology 2021Quote: ... the sample(s) were pre-incubated with protein G-coupled magnetic beads (Invitrogen) for 1 hour ...
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bioRxiv - Bioengineering 2019Quote: ... This cell line was derived from CHO-S cells (Gibco, Catalog No. A11364). In order to find the best combination of basal media and feed that could result in optimum cell growth ...
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bioRxiv - Genetics 2019Quote: Transfections were performed with the sGCSF vector following ExpiCHO-S manuals (Life Technologies Corporation ...
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bioRxiv - Plant Biology 2021Quote: ... and incubated for 30 s with SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific). Detection of chemiluminescence signal was performed with Molecular Imager ChemiDoc XRS+ (Bio-Rad).
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bioRxiv - Bioengineering 2021Quote: ... Prepared hybridization cocktails were applied to Clariom™ S assay (Thermo Fisher Scientific). The assays were washed and stained using the GeneChip® Fluidics Station 450 (Fluidics Protocol FS450_0001) ...
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bioRxiv - Biochemistry 2021Quote: ... in the super-resolution mode using EPU software (ThermoFisher Scientific, U. S. A.). Movies were collected with a defocus range of −0.8 to −2.6 μm at a magnification of 81000 x ...
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bioRxiv - Microbiology 2020Quote: ... containing 2% FBS (Hyclone) and 100 U/ml Penicillium-Streptomycin (P/S; Gibco) were seeded in each well of black μCLEAR flat-bottom 96-well plate (Greiner Bio-one™) ...
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bioRxiv - Systems Biology 2021Quote: ... RNA was extracted and assayed using Affymetrix Clariom S chips (Thermo Fisher Scientific). The microarray data are available at the Gene Expression Omnibus database (http://www.ncbi.nlm.nih.gov/geo/) ...