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Citations for Thermo Fisher :
3301 - 3350 of 10000+ citations for Bcl2 Like Protein 14 BCL2L14 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... Protein concentration was quantified using the Pierce BCA Protein Quantification kit (ThermoFisher #23225). 20 μg of protein extract and 5 μL of WesternSure Pre-stained Chemiluminescent Protein Ladder (Li-cor #926-98000 ...
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bioRxiv - Developmental Biology 2024Quote: ... and protein concentration was determined using the Pierce BCA Protein Assay Kit (ThermoFisher). To detect mitochondrial protein expression ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using a BCA protein assay (Thermo Fisher Scientific, 23227). Equal amounts of 10 μg protein lysate were loaded and separated on a 4-12% SurePAGETM gel (GenScript ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were determined using a BCA Protein Assay Kit (Thermo Fisher Scientific) with bovine serum albumin as the standard protein.
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated on Nupage® 4–12% Bis-Tris Protein Gels (Invitrogen) and transferred on polyvinylidene fluoride (PVDF ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentrations were determined with the Pierce BCA Protein Assay Kit (Thermo Scientific). SDS-PAGE and wet transfer Western blotting were performed ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was quantified using a Pierce BCA Protein Assay (Thermo Fisher Scientific) according to the manufacturer instructions ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: ... Total protein concentrations were determined by bicinchoninic acid Protein Assay (Thermo Fisher Scientific). Protein lysates were size-fractionated by SDS-PAGE and electro transferred to a PVDF membrane in a wet blotting system for 1.5 h at 45 V ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was quantified using a Pierce BCA protein analysis kit (Thermo Fisher), and then 20μg of protein was run on NuPage 4-12% Bis-Tris gels (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were quantified with BCA Protein Assay Kit (Thermo Fisher Scientific, A53226). Protein concentrations were quantified by BCA analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein levels were quantified using the Pierce BCA Protein Assay Kit (Thermofisher #PI23225) and equilibrated before FLAG tagged proteins were immunoprecipitated using anti-Flag M2 agarose beads (Sigma #A2220).
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). SDS was then added to lysates and incubated at 95 °C for 5 min ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was determined using the Qubit protein assay kit (ThermoFisher Scientific, Q33211).
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bioRxiv - Neuroscience 2024Quote: ... and VMH protein lysates were determined using a BCA protein assay (Thermo Fisher). Samples were probed with antibodies against UCP1 (Thermo Fisher ...
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bioRxiv - Physiology 2024Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay (Cat# 23235, Thermofisher), following the manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... Proteins were quantified using commercially available Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was quantified using a PierceTM BCA Protein Assay (ThermoFisher Scientific, 237227) and frozen at -80°C until downstream analysis.
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bioRxiv - Neuroscience 2024Quote: ... protein concentration was quantified using a PierceTM BCA Protein Assay (ThermoFisher Scientific, 237227) and frozen at -80°C until downstream analysis.
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were measured using a BCA Protein Assay Kit (Thermo Fisher Scientific), alongside a BSA protein standard (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... total protein concentration was quantified using BCA Protein assay (Thermo Fisher Scientific, Inc.). A total of 30 µg of proteins in breastmilk samples were separated on 8 or 10% Tris-Glycine gels (Titan Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was quantified using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific), and 10 μg electrophoresed in 4–12% Bis-Tris denaturing gels (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was isolated using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific), and Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... Protein concentration was determined with the bicinchoninic acid (BCA) protein assay kit (ThermoFisher). Lysate (40-50 µg ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentration was measured using the detergent-compatible (BCA) protein assay (Thermo Scientific). Denatured lysates were separated on NuPage 8-16% Tris-glycine Midi Protein gels (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration was measured using Pierce™ BCA Protein Assay Kit (Thermofisher Scientific) and normalized to 1 mg/mL ...
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bioRxiv - Cell Biology 2024Quote: ... Bulk proteins were visualized with the No-Stain Protein Labeling Reagent (#A44717, ThermoFisher) and imaged using the iBright Imaging System (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was quantified using the Micro BCA Protein Assay Kit (Thermo Fisher Scientific). Lysates were subjected to SDS-PAGE electrophoresis and transferred to PVDF membranes using Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were determined by BCA Protein Assay Kit (Thermo Fisher Scientific, 23225), and 30 µg of the lysates were collected as input ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were determined by BCA Protein Assay Kit (Thermo Fisher Scientific, 23225), and 30 µg of lysate was collected as input ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations were determined by BCA Protein Assay Kit (Thermo Fisher Scientific, 23225) and 500 μg of lysates were incubated overnight at 4 °C on a rotating wheel with anti-HA antibody (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantification of protein concentration was processed using the BCA protein assay (Thermo fisher). A total protein at concentration per lane at 50 μg was loaded into the 10% SDS-PAGE and then transferred to PVDF membranes ...
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bioRxiv - Physiology 2022Quote: ... Protein abundance was normalized to total protein using Pierce™ Reversible Protein Stain Kit for PVDF Membranes (cat 24585; Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... and supernatant removed for protein concentration analysis using a BCA protein assay (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific; cat # 23227). Twenty or 30 µg samples were loaded into SDS-PAGE gels and transferred onto either Amersham Hybond-P 0.45 µm PVDF membranes (GE Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... Purity and integrity of all proteins were checked and protein concentrations estimated using quantitative densitometry on GelCode Blue-stained protein gels (Thermo Fisher Scientific) containing bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatants were kept and protein concentration was estimated using the micro-BCA Protein Assay Kit (Pierce BCA Protein Assay Kit (Thermo Scientific: 23225)) ...
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was measured using the Pierce 660nm Protein Assay Kit or the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific). For each sample ...
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bioRxiv - Immunology 2022Quote: ... and B cells (gating strategy shown in Supplement to Figure 1-Figure 2B and Supplement to Figure 4-Figure 2A) included Fixable Viability Dye-eFluorTM780 (ThermoFisher 65-0865-14); mouse α-pig CD45 (BioRad MCA1222GA ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were then sectioned at −17°C with a cryostat at 14 μm and mounted onto Superfrost Utra Plus slides (Thermo Scientific; Cat# J4800AMNZ). Coronal cerebellar sections were collected from bregma −5.80 mm and −6.80 mm ...
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bioRxiv - Cell Biology 2021Quote: a-DIC (mouse monoclonal IgG2b, Santa Cruz #sc-13524 forw WB and IP; mouse monoclonal IgG2b, Thermo Fisher #14- 97772-80 for IF)
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bioRxiv - Neuroscience 2019Quote: ... Brains were sectioned at −17°C with the cryostat at 14 µm and mounted onto Superfrost Utra Plus slides (Thermo Scientific; Cat# J4800AMNZ). Sections of brain were collected for the whole cerebellum from bregma −5.80 mm and −6.80 mm ...
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bioRxiv - Immunology 2021Quote: ... flat-bottom 96-well plates (Immunulon 4 HBX) were coated with 0.25μg/mL anti-IFN-γ (AN-18, Invitrogen Ref #:14-7313-85) at 4°C overnight ...
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bioRxiv - Genomics 2021Quote: ... Macrophages were cultured by plating 14*106 PBMC resuspended in 2 ml macrophage media (Macrophage-SFM [with L-Glutamine without Antibiotics], Fisher Scientific UK LTD). After 1h 30’ non adherent cells were removed and 1 ml fresh macrophage media added together with 400 μl of autologous serum ...
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bioRxiv - Neuroscience 2020Quote: ... Transcription levels were determined in a 14 mL volume in duplicate using SYBR Green (Applied Biosystem, Foster City, CA) and 7500qPCR (Thermo Fisher, Waltham, MA). We analysed transcription levels of ImpL2 ...
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bioRxiv - Cancer Biology 2020Quote: ... After 24 hours cells were treated once with the indicated doses of Bortezomib followed by culturing for 14 days in DMEM medium (Life Technologies, Darmstadt, Germany) supplemented with 10% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 10 × 106 frozen PBMC from 13 convalescent donors were prepared in Falcon® 5ml-round bottom polystyrene tubes at a final concentration of 14 × 106 cells/mL in RPMI 1640 medium (Gibco by Life Technologies, #11875-093) supplemented with 10% of fetal bovine serum (Seradigm ...
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bioRxiv - Bioengineering 2020Quote: ... During the course of the experiment, a syringe driver (Harvard, MKCB2159V) was used to deoxygenate the blood with 0.03 % w/v sodium hydrosulfite (ACROS Organics 7775-14-6) in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were cultured at density of 10 embryos in 10 µl KSOM medium supplemented with 3 mg bovine serum albumin/mL (14) in 60-well plates (LUX 5260, Nunc, Naperville, IL, USA) overlaid with 2 mm of heavy paraffin oil (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... primary cells were seeded on Mattek 35-mm dishes (P35G-1.5-14-C) overnight and incubated for 30 minutes in pre-warmed live imaging staining solution (ThermoFisher Scientific, catalog number: A14291DJ) containing 100µM MitoTracker reagent ...
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bioRxiv - Cancer Biology 2019Quote: ... Three 15 amino acid peptides (P1, P3 and P4) and one 14 amino acid peptide (P2) were synthesized (Thermo Fisher Scientific, Rockford, IL). The purity of the peptides was >95% and each peptide was modified by N-terminal acetylation and C-terminal amidation to enhance their stability.
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bioRxiv - Cancer Biology 2019Quote: ... supplemented with 10% (KG-1, HL-60) or 20 % (MOLM-14, KASUMI-1) heat inactivated fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific), 2 mM L-glutamine (Sigma-Aldrich) ...