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Citations for Thermo Fisher :
3301 - 3350 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... overnight at 4°C and with secondary antibodies (Alexa fluor, Invitrogen 1:800) during 1h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) was purchased from ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... at 4 °C overnight or rabbit anti-HA (Invitrogen #MA5-27915, 1:1000) at 4°C over two nights ...
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bioRxiv - Developmental Biology 2023Quote: ... and mouse monoclonal anti-Elavl3/4 IgG2b (Invitrogen Molecular Probes, A21271, 1:250). The following secondary antibodies were used from Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... and mouse monoclonal anti-Elavl3/4 IgG2b (Invitrogen Molecular Probes, A21271, 1:250). The following secondary antibodies were used from Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated overnight at 4°C with AlexaFluor-568 streptavidin (1:2000, Invitrogen) and anti-somatostatin antibody (1:500 ...
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bioRxiv - Genomics 2020Quote: ... followed by AlexaFluor 647 F(ab’)2 goat anti rabbit IgG (H+L) (Invitrogen), CD3 (Clone UCHT1 ...
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bioRxiv - Biophysics 2020Quote: ... and Alexa Fluor 633 goat anti-chicken IgG (H+L) 2 mg/ml (Invitrogen) in PBS with 10% FBS and 0.1% saponin ...
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bioRxiv - Immunology 2022Quote: ... kept on ice for 2 h and induced with 0.25 mM IPTG (ThermoFisher, R1171) overnight at 25 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... the blot was incubated for 2 h in Superblock blocking buffer TBS (Thermo Scientific), followed by incubation with a 1:5000 dilution of IRDye800CW Streptavidin (LI_COR Bioscience ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were incubated with Alexa Fluor-conjugated secondary antibodies for 2 h (Invitrogen). Microscopic images of the stained sections were obtained with a Nikon 800 fluorescent microscope using Nikon imaging DS-R12 color cooled SCMOS ...
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bioRxiv - Molecular Biology 2023Quote: ... and followed by another 2 h incubation with Dynabeads MyOne Streptavidine C1 (62001, ThermoFisher). The beads were washed twice with low and high salt buffers at 37C ...
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bioRxiv - Immunology 2023Quote: ... and F(ab’)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed-APC (Invitrogen). eFluor-780 Fixable Viability Dye (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... then they were incubated for 2 h in 5% normal donkey serum (NDS, Gibco), 0.2% Triton-X100 in PBS to block nonspecific binding ...
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bioRxiv - Neuroscience 2023Quote: ... A-11001)—for 2 h followed by staining in DAPI (Thermo Fisher Scientific, Catalog no. EN62248) for 1 min ...
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bioRxiv - Molecular Biology 2021Quote: ... was cultured in Dulbecco’ ss Modified Eagle Medium (DMEM) supplemented with 7% fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic (Gibco) at 37°C with 5% CO2.
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bioRxiv - Molecular Biology 2021Quote: ... Reactions with RNase T2 were done at room temperature for 10 minutes in 1× Structure Buffer (10 mM Tris pH 7, 100 mM KCl and 10 mM MgCl2; Ambion). Nuclease S1 cleavage was carried out for 15 minutes at room temperature in 1× Reaction Buffer (40 mM sodium acetate pH 4.5 ...
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bioRxiv - Genetics 2022Quote: ... were combined and concentrated to ∼1 mL and dialyzed using 7 KMWCO Snake Skin dialysis tubing (Thermo Scientific, Waltham, Massachusetts) into 20 mM Tris 150 mM NaCl pH 8.4 ...
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bioRxiv - Biochemistry 2021Quote: 3 biological replicates of wild type and 2 different clones METTL5-KO HeLa cells (clone 1 and 7) were grown to confluency prior to lysis in Trizol (Invitrogen). Total RNA was purified as described above (‘RNA purification’ ...
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bioRxiv - Immunology 2024Quote: ... or 96-well flat-bottom plates at a concentration of 7 × 105 – 1 × 106 T cells/mL in T cell media with 10 U/mL rhIL-10 (Gibco). For both workflows ...
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bioRxiv - Microbiology 2023Quote: ... Five to 10 μg of total RNA were separated on a 7% (v/v) polyacrylamide gel (7.3 M urea, 1× TBE) and compared to RiboRuler LowRange RNA ladder (ThermoFisher Scientific). RNA from the gel was then blotted on a nylon membrane (Roche ...
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bioRxiv - Immunology 2024Quote: ... Each well was aspirated of residual cell culture media and incubated for 7 minutes at 37°C with 1 mL 1X TrypLE (Gibco) to detach the cells from the plate ...
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bioRxiv - Bioengineering 2024Quote: ... 7–35) were routinely grown as described with high glucose (4.5 g/L) and 1% (v:v) GlutaMax (Thermo Fisher Scientific), supplemented with 10% (v:v ...
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bioRxiv - Biochemistry 2024Quote: ... and 17 µL of 1x RNA Sequencing Buffer (20 mM sodium citrate pH 5, 1 mM EDTA, 7 M urea) (Ambion). The mixture was split across three tubes (9/8/8 µL) ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was then nutated overnight at 4 °C with 1 ml of Protein G Sepharose 4 Fast Flow slurry (Invitrogen) per 25ml of supernatant ...
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bioRxiv - Neuroscience 2020Quote: ... free-floating coronal hippocampal slices of 50 µm thickness were produced and incubated overnight in staining solution (4% normal goat serum, 0.4% Triton-X 100 and 4% BSA) with anti-GFAP antibody (1:1000, ThermoFisher, rat) and anti-Iba1 antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: Handpicked islets (~180) from control (n=4) and diabetic (n=4) mice were placed into 1 mL Trizol (Cat# 15596018, Ambion) and processed for RNA extraction ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% (KCL-22, K562, REH, NB-4) or 20% (MOLT-4, OCI-AML3, MOLM-13, Kasumi-1) fetal bovine serum (Gibco) and 1% penicillin–streptomycin (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... tissues were washed 3x for 1 hr in adult brain wash solution and incubated overnight at 4°C or for 4 hr at room temperature with AlexaFluor488 anti-rat (1:250) (Invitrogen) and DAPI (1:250 ...
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bioRxiv - Physiology 2023Quote: ... For cell viability assays 7-AAD (7-Aminoactinomycin D, Invitrogen, A1310) and Hoechst 33342 (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... and incubated with secondary antibodies including DAPI (0.1 µg/ml) for 1 h at RT in the dark: Alexa Fluor 488 (Thermo Fisher Scientific, 1:300, sheep/mouse/rabbit), Alexa Fluor 568 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... sections were washed 2 times for 8 minutes in DPBS containing 0.3% Triton X100 and then incubated for 1 hour in blocking solution containing the secondary antibody (1/200, Invitrogen P10994 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed with PBS and incubated for 1 h with Alexa Fluor 568 or 488-conjugated secondary antibodies (Thermo Fisher Scientific, Rockford, IL; 1:500), Alexa Fluor 488 Phalloidin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... PVDF membranes were blocked overnight in 0.5% fish gelatin in TBST solution and incubated for 1 h with horse radish peroxidase (HRP)-conjugated streptavidin (PierceTM High Sensitivity Streptavidin-HRP, ThermoFisher Scientific, Waltham, MA, USA, 1:20,000). Blots were imaged using the ClarityWestern ECL substrate (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... the top and middle membranes were incubated at RT for 1 h with HRP-conjugated anti-mouse secondary antibody (Thermo Fisher Scientific, USA; #31430, 1:8,000) and the lower part of the membrane with HRP-conjugated anti-rabbit secondary antibody (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... sections were washed two times for 8 min in DPBS containing 0.3% Triton X100 and then incubated for 1 hr in blocking solution containing the secondary antibody (1/200, Invitrogen P10994 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody ...
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bioRxiv - Neuroscience 2023Quote: The brains of P9-11 C57 mice were immersed and fixed for 3 days in cold 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide HCl (EDC, Thermo Fisher Scientific, in 0.1 M PB, pH 7.4) at 4°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Isolated B cells were activated at 1 x 106 cells mL-1 for 2 days in IMDM medium (ThermoFisher) with 10% fetal bovine serum ...
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bioRxiv - Genomics 2020Quote: ... 0.5-1×106 proliferating TILs were seeded with 100×106 normal donor peripheral blood mononuclear cells irradiated with 5,000 rad in a 1:1 mixture of TIL media and AIM-2 media (ThermoFisher), supplemented with 30 ng/mL OKT3 (Miltenyi Biotec ...
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bioRxiv - Genomics 2021Quote: ... After 2 days of transduction hNGN2-eGFP media was replaced with 1:1 ratio of Neurobasal A (ThermoFisher, 10888022) and DMEM/F12 medium containing GlutaMAX ...
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bioRxiv - Biochemistry 2020Quote: ... air-drying overnight and incubating with 2 μM TOTO-1 iodide (1 mM solution in DMSO, Thermo Fisher Scientific) for 15 min.
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bioRxiv - Microbiology 2020Quote: ... The monolayers were then overlaid with 1 to 2 ml of a mixture of 1 part 0.6% agarose (Invitrogen) and 1 part complete Eagle Minimal Essential Medium (VWR ...
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bioRxiv - Plant Biology 2019Quote: ... Rh (10 µg L-1) and Ir (5 µg L-1) in 2% trace analysis grade (Fisher Scientific, UK) HNO3 ...
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bioRxiv - Microbiology 2021Quote: ... 800 µl of isolation medium (DMEM, supplemented with 2% FBS, 1% penicillin-streptomycin and 1% amphotericin B, ThermoFisher Scientific) was added to each well ...
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bioRxiv - Physiology 2020Quote: ... the medium was switched to DMEM/F12 (1:1) plus 2% New Zealand fetal bovine serum (Thermo Fisher Scientific), hormones ...
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bioRxiv - Immunology 2021Quote: ... at 1:0.5:2 molar ratio using Lipofectamine™ 2000 (0.6 ul per 1 ug DNA; Invitrogen™, 11668019); growth medium was refreshed 16 h after transfection ...
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kinesin recruitment by adapter SKIP on melanosomes is dynamically controlled by LC3B phosphorylationbioRxiv - Cell Biology 2021Quote: ... The tumor was excised and was washed in homogenization buffer (0.25 M sucrose [Merck, CAS 57–50–1], 10 mM HEPES, 1 mM EDTA, 2% antibiotic and antimycotic [ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 h at room temperature and stained for 10 min with 4,6-diamidino-2-phenylindole (DAPI; 1 mg/ml stock solution diluted 1:50,000 in PBS; Thermo Fisher) at room temperature with at least four PBS washes in-between ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 1 mL nuclei wash buffer (2% BSA and 0.2LULul−1 RNase inhibitor (Thermo Fisher, cat. no. AM2694) in 1X PBS ...
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bioRxiv - Biochemistry 2024Quote: ... and p976 were packaged in HEK-293T cells at a ratio of 2:1:1 by Lipofectamine 2000 (Invitrogen). The ratio of liposome to DNA was 2:1 ...