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Citations for Thermo Fisher :
3251 - 3300 of 7745 citations for Recombinant Human PPIA His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Immunology 2021Quote: PBMC derived CD4+ T cells were isolated from HIV-infected MACS participants using EasySep™Human CD4+ T Cell Isolation Kit and activated overnight with Human T-Activator CD3/CD28 Dynabeads® (Life Technologies). The next day Dynabeads were separated from the CD4+ T cells by manual dissociation followed by magnet isolation ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
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bioRxiv - Cell Biology 2022Quote: Human cerebral organoids are generated from human induced pluripotent stem cells (iPSCs) which were purchased from GibcoTM (Gibco by Thermofisher, Waltham, Massachusetts, USA). iPSCs were plated in a dish pre-coated with Matrigel® hESC-Qualified Matrix (Corning ...
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bioRxiv - Cell Biology 2023Quote: We profiled the expression of transcripts in human aortic vascular smooth muscle cells using the Clariom D human DNA microarray (Thermo Fisher Scientific Inc.). This platform includes information from probe sets representing known and predicted exons and introns on both strands of the genome that have been mapped to more than genes ...
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bioRxiv - Immunology 2023Quote: Customized cytokine 25-Plex Human ProcartaPlex Assay (customized from Cytokine 25-Plex Human ProcartaPlex Panel 1B with the catalog # EPX250-12166-901, Thermo Fisher Scientific, USA) was used to measure multiple cytokines in the supernatants of stimulated human PBMCs.
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bioRxiv - Cell Biology 2020Quote: ... Established BMEC lines were transduced with GFP-tagged RNAi lentivirus (BLOCK-iT HiPerform Lentiviral Pol II miR RNAi Expression system, Invitrogen Cat# K4934-00) targeting Raptor or Rictor ...
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bioRxiv - Molecular Biology 2021Quote: ... coupled to a Q Exactive hybrid mass spectrometer (Thermo) (N-terminal FLAG-tagged datasets) or an Orbitrap Fusion Lumos Tribrid (Thermo Fisher Scientific, Waltham, MA) mass spectrometer (C-terminal FLAG-tagged datasets) ...
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bioRxiv - Developmental Biology 2019Quote: ... C- and N-terminal GFP-tagged Nubp1 and Nubp2 expression plasmids [32] were transfected using Lipofectamine® 3000 (Thermo Fisher Scientific, Waltham, MA, USA) and imaged using a Nikon C2 Confocal microscope.
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bioRxiv - Biochemistry 2019Quote: ... Site-directed mutagenesis of plasmids to generate the C-terminal Myc tagged ECH1 catalytic mutants and the C-terminal V5 tagged UCP3 truncation mutants was carried out with Platinum pFX DNA polymerase from (Life Technologies, Grand Island, NY), following the manufacturer’s protocols for site-directed mutagenesis ...
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bioRxiv - Genomics 2022Quote: ... we performed end repair and then selectively purified biotin-tagged DNA using streptavidin coated beads (DYNAL™ MyOne™ Dynabeads™ Streptavidin C1, Invitrogen 65001). A-tailing and Illumina TruSeq adapter ligation (Illumina ...
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bioRxiv - Biophysics 2023Quote: ... Total tubulin was labeled using a primary antibody against alpha-tubulin and a Cy3-tagged secondary antibody (Thermo Fisher Scientific Inc., Waltham, MA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were further incubated for 2 h at room temperature with HRP-tagged secondary antibody of goat anti-mouse (1:1000) (Life Technologies, Thermo Scientific, USA). The sections were then washed with TBST three times ...
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bioRxiv - Cell Biology 2023Quote: Flag-tagged Parkin was inserted into pMSCV vector by using the pCR®8/GW/TOPO® TA Cloning® Kit (Thermo Fisher Scientific). To perform the pCR®8/GW/TOPO® cloning ...
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bioRxiv - Genomics 2022Quote: ... the HA-tagged protein of interest was detected with 1:500 diluted HA Tag Alexa Fluor 647 conjugated mouse antibody (ThermoFisher, Catalog no. 26183-A647). After antibody incubation ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor™ 488-tagged anti-SUMO2/3 antibody was labelled using an Alexa Fluor® 488 Antibody Labeling Kit (Invitrogen-ThermoFisher, Waltham, MA). Unless otherwise specified ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa Fluor™ 488-tagged anti-SUMO2/3 antibody was labelled using an Alexa Fluor® 488 Antibody Labeling Kit (Invitrogen-ThermoFisher, Waltham, MA). Unless otherwise specified ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids encoding fluorescently tagged mutant CMV 2b proteins were generated using Gateway® cloning following the protocol provided (Invitrogen, Thermo-Fisher Scientific, Paisley UK) with attB-flanked DNA fragments cloned into the pDONR221 donor plasmid ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmids encoding fluorescently tagged mutant CMV 2b proteins were generated using Gateway® cloning following the protocol provided (Invitrogen, Thermo-Fisher Scientific, Paisley UK) with attB-flanked DNA fragments cloned into the pDONR221 donor plasmid ...
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bioRxiv - Systems Biology 2020Quote: ... An Ion Torrent PGM was used with the Hi-Q 400 template preparation and sequencing kit and a 318v2 sequencing chip (Thermo Fisher, MA). All the quality control steps were performed using Bioanalyzer 2100 (Agilent ...
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bioRxiv - Molecular Biology 2019Quote: The pFastBacNKI-his-3C-m6A-Tracer construct was used for creation of baculovirus according to the Bac-to-Bac system (Thermo Fisher Scientific). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... of 8 mice were then resuspended in 10 ml DMEM supplemented with 10 % heat inactivated new born calf serum (HI NCBS, LifeTechnologies / Gibco, # 26010-074) and plated on two 10 cm dishes (Corning ...
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bioRxiv - Genetics 2021Quote: One microliter of GlobalFiler™ or GlobalFiler™ Express amplified product was added to 9.5 μL Hi-Di™ formamide (ThermoFisher Scientific) and 0.5 μL GeneScan™ 600 LIZ® size standard (ThermoFisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... the SBT3.8 ORF was amplified from genomic DNA with a reverse primer including six His codons and first cloned into pCR2.1-Topo (Life Technologies, Carlsbad, CA). EcoRI sites from pCR2.1 were used for ligation into pART7 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Libraries were then pooled and loaded onto chips and sequenced on Ion Torrent PGM using Ion PGM Hi-Q Sequencing Kit (Thermo Fisher Scientific). Fast-q or BAM files from sequencing runs were analysed in Geneious ...
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bioRxiv - Plant Biology 2019Quote: ... with induction by 0.5 mM IPTG (Isopropyl β-D-1-Thiogalactopyranoside, 16°C, 12 h) and then purified using Ni-NTA His binding resin (Thermo Scientific) according to the manufacturer’s manual ...
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bioRxiv - Immunology 2019Quote: ... PCR product was fused by restriction enzyme-compatible ends with the CD8α-CD28-CD3ζ domain contained in the pcDNA3.1/V5-His TOPO TA (Invitrogen, Carlsbad, CA, USA). CD16158F-CR and CD32131R-CR were subcloned into NcoI and MluI sites of the SFG retroviral vector ...
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bioRxiv - Biophysics 2019Quote: ... the MerMAID1 gene was subcloned with a C-terminal TEV protease restriction site and a 6× His-Tag into the pPiCZ vector (Invitrogen, Carlsbad, CA). Zeocin™-resistant positive clones were selected from electroporation-transformed yeast cells ...
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bioRxiv - Cell Biology 2019Quote: ... conjugated with GFP at the C-terminus (Par-6-GFP and aPKC-GFP) was inserted into pAc5.1/V5-His B plasmid (Invitrogen, Thermo Fisher Scientific). To construct an expression vector for Par-3 under control of the Gal4-UAS system ...
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bioRxiv - Genetics 2019Quote: ... using 1 μl PCR product in 9.5 μl Hi-Di™ formamide containing 0.5 μl GeneScan™ 600 LIZ® Size Standard v2.0 (Thermo Fisher Scientific). One microliter of allelic ladder was included per 24 sample injection ...
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bioRxiv - Bioengineering 2021Quote: ... THP-1 cells were expanded in RPMI medium (ATCC® 30-2001™) supplemented with 10% heat inactivated fetal bovine serum (HI-FBS, Gibco, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: S1R was expressed with an N-terminal 6*His-tag fusion in Sf9 cells using Bac-to-Bac baculoviral expression system (Thermo Fisher Scientific) according to manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2020Quote: The Taq polymerase-amplified PCR product of the rdLRP was inserted into the TOPO Cloning site of a pcDNA3.1/V5-His-TOPO vector (Thermo Fisher Scientific, MA). The vector was transfected to 6×105 cells of HEK293T cells using Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: The full-length OpIE2 promoter was obtained from the pIB/V5-His by PCR using Phusion High fidelity DNA Taq polymerase (ThermoFisher Scientific, USA) according to the following program ...
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bioRxiv - Biophysics 2022Quote: ... followed by an 8× His tag and HRV 3C protease cleavage site was cloned into the modified pFastBac1 (Invitrogen, Carlsbad, CA, USA) vector ...
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bioRxiv - Molecular Biology 2022Quote: ... The sequencing was performed on Ion 318 v2 chip and Ion Torrent PGM machine employing the Ion PGMTM Hi-QTM View Sequencing kit (Life Technologies, USA). All procedures were carried out according to manufacturer’s instructions and each run was expected to produce approximately 150,000 reads per sample ...
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bioRxiv - Microbiology 2022Quote: ... and Omicron RBD-his protein were coated overnight at 4 °C in a 96-well plate (MaxiSorp Nunc-immuno, Thermo Scientific, USA). Wells were blocked with 5% non-fat milk (Biofroxx ...
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bioRxiv - Microbiology 2022Quote: ... and Omicron RBD-his protein were coated overnight at 4 °C in a 96-well plate (MaxiSorp Nunc-immuno, Thermo Scientific, USA). Wells were blocked with 5% non-fat milk (Biofroxx ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride membrane [36] and detected with the anti-His (C-terminal)-HRP antibody (Thermo Fisher/Invitrogen) according to the manufacturer's instructions ...
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bioRxiv - Biochemistry 2021Quote: ... through G-25 resin the peptide-mRNA:cDNA libraries were incubated with cross-linked linked SARS-CoV-2 Mpro (200 nM) immobilized on Dynabeads™ His-tag Isolation & Pulldown (Life Technologies) at 4 °C for 30 min after which the beads were washed with ice-cold selection buffer (3 x 100 μL) ...
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bioRxiv - Neuroscience 2020Quote: ... The ORF thus PCR-amplified was cloned into the pcDNA™3.1/V5-His TOPO™ TA vector (ThermoFisher Scientific, Cat # K480040) following manufacturer’s protocol.
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bioRxiv - Evolutionary Biology 2022Quote: ... 0.5 μl of fivefold-diluted PCR product was mixed with 9.25 μl of Hi-Di formamide and 0.25 μl LIZ600 size standard (ThermoFisher Scientific, Waltham, MA, USA), denatured for 10 min at 95°C and then immediately cooled on ice ...
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bioRxiv - Cell Biology 2019Quote: ... and FLAG-dDYNLL1 (EcoRI/XbaI) fragments were inserted into the S2-cell expression vector pAc5.1/V5-His-A (Thermo Fisher Scientific, V411020).
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bioRxiv - Molecular Biology 2019Quote: ... using 1 μl PCR product in 9.6 μl Hi-Di™ formamide containing 0.4 μl GeneScan™ 600 LIZ® Size Standard v2.0 (Thermo Fisher Scientific). One microliter of allelic ladder was included per 24 sample injection ...
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bioRxiv - Microbiology 2019Quote: ... The lysates were run on a 15% PAGE and developed using monoclonal anti-His antibody (Biospecs, BTL1010) with super signal west femto maximum sensitivity substrate (Thermo Scientific, 34095).
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bioRxiv - Cancer Biology 2020Quote: ... with C-terminal twin-Strep-10x His dual tag followed by a stop codon was synthesized using GeneArt Gene Synthesis service (Invitrogen, Carlsbad, CA) with SgfI and MluI restriction sites at the beginning and the end of the ORF sequence ...
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bioRxiv - Genetics 2020Quote: The PCR products were diluted 1:1 with milliQ water and 1μL of the diluted PCR product was added to a 384-format genotyping plate containing 4μL of a mix of Hi-Di™ formamide (Applied Biosystems™) and Genescan™ 600 LIZ™ dye size standard v2.0 (Applied Biosystems™ ...
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bioRxiv - Immunology 2021Quote: ... an HRV3C protease cleavage site, a TwinStrepTag and Hexa-His-tag (McLellan et al., 2020) was transiently expressed in FreeStyle293F cells (Thermo Fisher scientific) using polyethylenimine (PEI ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat. #MA1-21315-1MG, Thermo Fisher Scientific) in PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... and T2/Onc2.r1 5’-ATC TAT GGC TCG TAC TCT ATA GGC-3’ designed to amplify the pT2/Onc2 vector excluding the MSCV 5’ LTR minimal promoter and Lun-SD sequences using platinum Hi-Fi DNA Taq polymerase (Invitrogen #11304-028) in 1X Hi-Fi PCR buffer (Invitrogen #52045 ...