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Citations for Thermo Fisher :
3251 - 3300 of 7808 citations for Recombinant Human F10 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... PE-Cy7 anti-human C27 (clone O323, ThermoFisher Scientific), AlexaFluor 700 anti-human CD38 (clone LS198-4-3 ...
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Metabolic specialization drives reduced pathogenicity in Pseudomonas aeruginosa CF clinical isolatesbioRxiv - Microbiology 2023Quote: ... previously coated with human type I collagen (Gibco, A1048301). ALI was established once cells reached full confluency by removing media from the apical chamber and replacing media in the basolateral chamber with Pneumacult-ALI maintenance medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2023Quote: ... 50 ng/mL human IL-21 (Thermo Fisher Scientific), 50 ng/mL human IL-4 (Miltenyi) ...
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bioRxiv - Neuroscience 2023Quote: ... human Bmp7 (100 ng/ml, ThermoFisher Scientific, 120-03P) or human Semaphorin3d (400 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... 2 ng/ml human Tgfb2 (ThermoFisher Scientific, 100-35B), 10 ng/ml murine Csf1 (Biolegend ...
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bioRxiv - Neuroscience 2023Quote: ... human Pleiotrophin (100 ng/ml, ThermoFisher Scientific, 450-15), human Midkine (100 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... human Midkine (100 ng/ml, ThermoFisher Scientific, 450-16), human Bmp7 (100 ng/ml ...
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bioRxiv - Cell Biology 2024Quote: ... primary human islets were first dissociated with TrypLE (Gibco) prior to incubation with a mouse monoclonal anti-human NTPDase3 antibody (Ectopeptidases ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... (Telangana, India) with human suspension hepatocytes (HMCS1S, Gibco USA). 200 µL of cell suspension containing 2 x 106 hepatocytes/mL (>95% viability ...
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bioRxiv - Systems Biology 2024Quote: ... ANXA1 (rabbit anti-human, 1:1000, ThermoFisher Scientific, UK), phosphotyrosine-21 ANXA1 (rabbit anti-human ...
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bioRxiv - Cell Biology 2024Quote: ... or two siRNAs targeting human PLA2G15 (ThermoFisher s24296, s24297) using RNAiMax (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... human astrocytes at P4 (K1884, Thermo Fisher Scientific Gibco) were seeded in each well ...
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bioRxiv - Neuroscience 2024Quote: ... human astrocytes at P4 (K1884, Thermo Fisher Scientific Gibco) were seeded in each well ...
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bioRxiv - Neuroscience 2024Quote: ... for human HTT and Alexa fluor 647 (Thermofisher # B40958) for mouse Htt ...
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bioRxiv - Neuroscience 2024Quote: ... AT8 human PHF-Tau (Thermo Scientific, MN1020, 1:250). The following secondary antibodies were used ...
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bioRxiv - Bioengineering 2024Quote: Human dermal fibroblast (HDF) cells (C0135C; ThermoFisher Scientific, UK) were cultured in flasks with Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit anti-human CD3 (clone SP7, Thermo Fisher Scientific); sheep anti-human CD34 (#AF7227-SP ...
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bioRxiv - Developmental Biology 2024Quote: ... in 2 mL complete human medium (DMEM/F12 [Gibco, Massachusetts ...
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bioRxiv - Immunology 2024Quote: ... Dynabeads™ Human T-Activator CD3/CD28 (ThermoFisher Scientific) were added to the culture ...
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bioRxiv - Microbiology 2024Quote: ... Goat anti-human IgG (H + L) (Thermo Fisher Scientific) or Goat anti-Human IgG Fc recombinant (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Goat anti-human IgG (H+L) (Thermo Fisher Scientific) pre-coupled to Alexa Fluor 647 were used as secondary antibodies in flow cytometry experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... Human PA-1 cells were cultured in MEM (Gibco) supplemented with GlutaMAX ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the human σ2 receptor was cloned into pcDNA3.1 (Invitrogen) mammalian expression vector with an amino-terminal protein C tag followed with a 3C protease cleavage site and transfected into Expi293 cells (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... RT-PCR was performed to obtain cDNA using Superscript III reverse transcriptase and RNaseOUT recombinant ribonuclease inhibitor (Invitrogen). QPCR was further performed using Bio-Rad SsoAdvanced universal SYBR green supermix on the Biorad CFX Connect real-time PCR system ...
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bioRxiv - Molecular Biology 2020Quote: ... Immulon 2 HB plates were coated with 2μg/ml Pierce recombinant protein A/G (ThermoFisher catalog no: 77677) or purified SARS-CoV-2 receptor binding domain (provided by Florian Krammer ...
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was collected 6 days later and recombinant mAb was purified using protein A agarose (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: Recombinant STUB1 protein (aa25-aa153) was dialyzed overnight with Slide-A-Lyzer cassette (7K MWCO, Thermo Scientific, #66373) in 1 liter of dialysis buffer (PBS ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant constructs were transfected into HEK293 cells either individually or together using Lipofectamine® 3000 reagent (Invitrogen) following the instructions provided ...
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bioRxiv - Immunology 2022Quote: ... Recombinant antibodies were produced by transient co-transfection of Freestyle™ 293-F suspension cells (Thermo Fisher Scientific) using PEI-precipitation method as previously described (Lorin and Mouquet ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant protein was expressed in the Pichia pastoris strain SMD1168H (Philosof et al., 2017) (Thermo Fisher Scientific). The cells were harvested 48–60 h after expression was induced in BMMY medium when 10 mM of all-trans-retinal (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant protein was captured on a Ni2+-NTA HisPur® affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... Expression and purification of recombinant proteins were performed according to manufacturer’s instructions (Invitrogen Corporation, Catalog no. K1710-01): YPD medium supplemented with 100 μg ml−1 zeocin was used for initial growth of P ...
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bioRxiv - Neuroscience 2020Quote: To label purified recombinant tau (monomeric or aggregated tau P301S) with Dylight 488 NHS ester (Thermo Fisher Scientific) or pH-sensitive form of rhodamine (pHrodo ...
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bioRxiv - Immunology 2022Quote: ... The recombinant product was transformed into MAX Efficiency™ Stbl2™ Competent Cells (Thermo Fisher Scientific, MA, USA) to generate the pNDV-HXP-S rescue plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... HMLE.M cells were induced after prolonged treatment with 2.5 ng/ml recombinant TGFβ1 (Life Technologies, Cergy-Pontoise, France). Cell proliferation and survival analyses were performed in 3 or more independent experiments ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 receptor binding domain was coated onto MaxiSorp 96-well plates (Thermo Scientific, Cat # 442404) at 200 ng/well at 4 °C overnight ...
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bioRxiv - Biochemistry 2020Quote: The soluble recombinant protein was captured on a Ni2+-NTA affinity resin by gravity flow (Thermo Fisher Scientific). Unbound proteins were washed with 25 mM HEPES ...
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bioRxiv - Synthetic Biology 2019Quote: ... The soluble recombinant MDH mutants were purified by using HisPur™ Ni-NTA Spin columns (ThermoFisher Scientific, USA). The columns with loaded supernatants were washed with Wash buffer (0.1 M Potassium phosphate buffer ...
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bioRxiv - Immunology 2021Quote: ... Dinutuximab and dinutuximab LALA-PG were then purified using recombinant protein A-Sepharose 4B (ThermoFisher Scientific, Waltham, MA), buffer exchanged into antibody buffer (20mM L-Histidine ...
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bioRxiv - Immunology 2020Quote: ... Recombinant viruses were generated in Vero E6 cells (ATCC-CRL1586) grown in DMEM high glucose media (Gibco #11995065) supplemented with 10% Hyclone Fetal Clone II (GE #SH3006603HI) ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μg/mL gentamicin (Wisent) and 5 ng/mL recombinant mouse fibroblast growth factor basic (FGF-b, ThermoFisher). At ~90% confluence ...
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bioRxiv - Immunology 2022Quote: ... Recombinant baculoviruses were produced using the Bac-to-Bac baculovirus expression system according to the manufacturer’s manual (Invitrogen).The scheme for constructing the recombinant baculoviruses ...
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bioRxiv - Microbiology 2022Quote: ... Bacmids and recombinant baculoviruses were generated from the pFastBac Dual vectors using the Bac-to-Bac system (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: The recombinant proteins named rS1Beta was purified using a CaptureSelect™ C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guidelines ...
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bioRxiv - Pathology 2023Quote: Tissue microarrays were stained using the GPER recombinant rabbit monoclonal antibody clone:20H15L21 (Thermo Fisher Scientific, catalog #703480) by DCL Pathology (Indianapolis ...
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bioRxiv - Neuroscience 2022Quote: ... Triturated tissue was then digested for 5 min using the recombinant protease TrypLE Express (ThermoFisher Scientific, cat. #12605) at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of RNA were treated with recombinant DNaseI using the DNA-free DNA Removal Kit (Ambion/ThermoFisher). The DNA-free RNA sample was then used for reverse transcription ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of RNA were treated with recombinant DNaseI using the DNA-free DNA Removal Kit (Ambion/ThermoFisher). The DNA-free RNA sample was then used for reverse transcription ...
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bioRxiv - Microbiology 2024Quote: The plasmids for the recombinant SARS-CoV-2 variants were transiently transfected into Expi293F cells (Thermo Fisher Scientific) with the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... coli NEB10 with DNA isolated from recombinant strains (via the Whole Blood Genomic DNA Purification Kit, Thermo Scientific) followed by selection on LB agar with the appropriate drug ...