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Citations for Thermo Fisher :
3251 - 3300 of 10000+ citations for 7 METHOXY 4 4 DIMETHYL 3 4 DIHYDRO 2H NAPHTHALEN 1 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... We used siDESIGN Center (Horizon Discovery) to design siRNA (siUNG#4) targeting the 3′UTR region of UNG mRNA (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: ... and probed overnight at 4°C with the following primary antibodies diluted in 1x TBS-T with 3% BSA (Fisher Scientific BP1600): α-SARM1 (BioLegend 696602 ...
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bioRxiv - Biochemistry 2024Quote: HEK-293 cells (ATCC, CRL-1573) were subcultured every 3-4 days in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, 41965-039) supplemented with 10% v/v fetal bovine serum (Sigma Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... These grids were blotted with filter paper for 3∼6 s (100% humidity at 4 °C) in a Vitrobot Mark IV (Thermo Fisher Scientific) and vitrified in liquid ethane at liquid nitrogen temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were then centrifuged at 1000rpm for 3 minutes at 4°C before being suspended in 100μl HBSS (ThermoFisher, Cat No. 14025) + 1% FBS ...
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bioRxiv - Biochemistry 2022Quote: ... followed by mechanical blotting for 3-4 s and rapid vitrification in liquid ethane with a Vitrobot Mark IV plunge-freezing device (Thermo Fisher Scientific) operated at 4 °C and 100 % relative humidity.
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bioRxiv - Molecular Biology 2023Quote: ... 7H11 plates contained 50 μg/mL hygromycin and 50 μM 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) (Thermo Scientific). Unless specified ...
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bioRxiv - Neuroscience 2023Quote: ... before being spun (5000rpm 4⁰C for 3 minutes) and homogenised (70 seconds with a pestle) in 300 ul trizol (Invitrogen 15596-026). RNA was extracted using the Zymo Direct-zol RNA Microprep Kit (Zymo Research ...
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bioRxiv - Pathology 2023Quote: Dried peptide samples were dissolved in 4.8 μL of 0.25% formic acid with 3% (vol/vol) acetonitrile and 4 μL of each sample was injected into an EasynLC 1000 (Thermo Fisher Scientific). Peptides were separated on a 45 cm in-house packed column (360 μm OD×75 μm ID ...
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bioRxiv - Genetics 2023Quote: ... Total RNA was extracted from n = 3–4 biological replicates per sample using the PureLink RNA Mini Kit (Invitrogen, Waltham, MA, USA), and sample quality was evaluated using a TapeStation ...
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bioRxiv - Cell Biology 2023Quote: ... Samples denatured by 3 min boiling in Laemmli buffer were separated by electrophoresis on 4-12% bis-acrylamide gel (Thermo Fisher Scientific), then transferred to 0.45 µm nitrocellulose membranes (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... cells were washed in Ca2+-free PBS and incubated for 20 min at room temperature with 3 μM Fluo-4-AM (F14201, Thermo Fisher Scientific) in PBS while being protected from ambient light ...
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bioRxiv - Neuroscience 2023Quote: ... Fibroblasts started to migrate out of the skin biopsies after 7-10 days and were transferred to two T75 flasks after 3-4 weeks using the standard splitting protocol with TrypLE™ Express enzyme (Thermo Fisher). Fibroblasts were then cultured in IMDM (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... Uterine tissues were cut into 3- to 4-mm lengths with sterile scissors and incubated in phenol red-free HBSS(−) containing dispase (#17105-041, Thermo Fisher Scientific) and pancreatin (#P3292 ...
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bioRxiv - Neuroscience 2023Quote: ... for a 35 mm dish 4 μl of Lipofectamine 2000 and selected plasmids (3 μg) were diluted in Opti-MEM medium (Thermo Fisher Scientific) to a final volume of 250 μl and incubated for 20 min at RT ...
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bioRxiv - Immunology 2024Quote: ... we co-transfected 3×106 3-day activated CD8+T cells with 150 pmol/106 cells of human TSP-4-specific siRNAs (#s14100, Thermo Fisher Scientific) and 1.5 μg/106 of the pMax-TSP-1-GFPSpark construct ...
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bioRxiv - Plant Biology 2024Quote: ... The indicated total protein amount was loaded on SDS-PAGE gels (4-20% Mini-protean® TGX™ precast gels, [Bio-Rad], NuPAGE 4-12% Bis-Tris gels [Thermofisher], or self-cast minigels) and blotted on PVDF Immobilon-P membrane (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Neuroscience 2021Quote: ... Mg-ATP 4, Na2-phosphocreatine 10, Na3-GTP 0.2 (pH 7.3, 295 mosmol.kg-1) and 20 µM AlexaFluor488 (Invitrogen). Whole-cell patch-clamp recordings from identified interneurons 5IR_DIC ...
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bioRxiv - Cell Biology 2020Quote: ... Gonads were incubated overnight at 4°C with 1 U/ml Texas Red-X Phalloidin (ThermoFisher Scientific) in staining solution (1% BSA ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated overnight at 4°C with antibodies directed against CD44 (Thermo Scientific, MA4400; 1:200) and CD56 (Leica Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... a 1:1000 dilution of DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride, Molecular Probes; D1306, Molecular Probes)/PBS solution or 5 mg/ml Hoescht (Invitrogen)/PBS solution was carried out for 15 min at RT ...
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bioRxiv - Cell Biology 2020Quote: ... a 1:1000 dilution of DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride, Molecular Probes; D1306, Molecular Probes)/PBS solution or 5 mg/ml Hoescht (Invitrogen)/PBS solution was carried out for 15 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... ISF Aβ42 in the 14 samples for each mouse was measured by ELISA (Invitrogen, KHB3441, 1:4). To determine Aβ42 half-life (Cirrito et al. ...
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bioRxiv - Bioengineering 2019Quote: DSS-treated mice received 1% to 4% (weight/volume) DSS (40,000 MW, Alfa Aesar, Thermo Fisher, France) in drinking water for 5 days to induce an acute episode of colitis ...
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... and Nuclear DNA was counterstained with 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher). Alternatively ...
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bioRxiv - Bioengineering 2020Quote: ... UK) and the piggyBac-BE3-GFP plasmid at a 1:4 molar ratio using Lipofectamine 3000 (Invitrogen). At 48 h after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C with rabbit anti-PSD95 antiserum (1:1,000, Invitrogen/ThermoFisher) and guinea pig anti-vGlut2 antiserum (1:12,000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C with rabbit anti-PSD95 antiserum (1:1,000, Invitrogen/ThermoFisher) and guinea pig anti-vGlut2 antiserum (1:12,000 ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were stained with 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene p-toluenesulfonate (TMA-DPH; Molecular Probes) at a final concentration of 100 μM and the cells were then immobilized on a 2% agarose pad made with sporulation buffer covered with a no.1.5 coverslip ...
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bioRxiv - Neuroscience 2020Quote: ... was incubated overnight at 4 °C and anti-mouse-HRP-coupled secondary antibody (1:3000, Life Technologies) was incubated for 1.5 h at RT ...
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bioRxiv - Plant Biology 2021Quote: ... and incubated overnight at 4°C with monoclonal primary anti-GFP antibody from rabbit (1:2,000) (Invitrogen), and with secondary anti-rabbit antibody (1:30,000 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were rinsed in PBS 4 × 20 minutes and then incubated in Hoechst (1:2000; Thermo Fisher) for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was probed overnight at 4°C with monoclonal α-FLAG (#31430, ThermoFisher Scientific; 1:10,000) antibody diluted in 1×TBS-T buffer containing 3% bovine serum albumin ...
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bioRxiv - Biophysics 2020Quote: ... Nuclei were detected by staining with 4’,6-diamidino-2-phenylindole (DAPI, 1:200, ThermoFisher Scientific, USA) for 10 minutes at RT ...
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bioRxiv - Physiology 2021Quote: ... The sections were then incubated overnight at 4°C with polyclonal anti-laminin (1:1000; ThermoFisher Scientific) primary antibody diluted in blocking solution ...
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bioRxiv - Cell Biology 2021Quote: ... and 1% sodium bicarbonate) or selection medium (maintenance media supplemented with 4 μg/ml blasticidin (Thermo Fisher) and 2.5 μg/ml puromycin (Sigma-Aldrich)) ...
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bioRxiv - Microbiology 2022Quote: ... Supernatants were aspirated and pellets were resuspended in 3-5 µL of 1X PBS containing 0.02 mM 1-(4-(trimethylamino) phenyl)-6-phenylhexa-1,3,5-triene (TMA-DPH)(Invitrogen).Cells were mounted on glass slides with polylysine-treated coverslips ...
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bioRxiv - Microbiology 2022Quote: ... and L plasmids at a ratio of 4:2:2:2:1 using Lipofectamine 2000 (ThermoFisher Scientific). Cells were transfected in 6 well plates and subsequently transferred to T25 flasks with HEp2 cells until cytopathic effect (CPE ...
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bioRxiv - Genetics 2022Quote: ... at room temperature then incubated overnight at 4°C with mouse anti-v5 (1:1000, Invitrogen, RRID:AB_2556564), and rat anti-tubulin alpha (1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... and for 48 hours at 4°C followed by 15 minutes of DAPI staining (1:5000; ThermoFisher). Retina whole mounts were also incubated with rabbit anti-Alexa-fluor 488 conjugated GFP for 4 days at 4°C followed by 15 minutes of DAPI staining ...
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bioRxiv - Biochemistry 2020Quote: ... or PAF (4 μM) with or without sAGP-1 using competitive ELISA kit (EMSCAMPL, Invitrogen, Vienna, Austria). This system displays a detection sensitivity of 0.39 pmol/ml of cAMP according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... buffered with 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technology, Thermo Fisher Scientific Inc., USA) and coated with 20 μg/mL laminin (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... sections were counterstained with 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI, D3571, Molecular Probes, dilution 1:1000), re-washed ...
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bioRxiv - Neuroscience 2022Quote: ... overnight at 4 °C followed by goat anti-rabbit Alexa-488 (1:200, Invitrogen, Waltham, MA, USA) for 2 hours at room temperature and DAPI (1:100 ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with primary antibodies overnight at 4 °C (1:100 mouse anti CYP3A4 antibody, Life Technologies MA5-17064 ...
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bioRxiv - Systems Biology 2022Quote: ... The samples were centrifuged at 100,000×g for 1 h at 4°C (S55A2 rotor, Thermo fisher). The pellets were resuspended in SDS sample buffer and subjected to western blot analysis.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... stock solutions were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The thrombin-selective inhibitor PPACK.2HCl and MMP Inhibitor V (ONO-4817 ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight 4°C incubation with rabbit polyclonal antibodies against claudin-5 (1:100; Life Technologies) and ZO-1 (1:200 ...
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bioRxiv - Cell Biology 2019Quote: ... Neurons were cultured for 1-4 days in vitro (DIV) and transfected with Lipofectamine 3000 (L3000; Invitrogen) for 48 h (DIV 3-6 ...