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Citations for Thermo Fisher :
3201 - 3250 of 6996 citations for THBS1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 20 ng/ml human EGF recombinant protein (Gibco, PHG0314), 20 ng/ml human basic FGF recombinant protein (bFGF ...
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bioRxiv - Neuroscience 2024Quote: ... Rabbit anti-Human IgG DyLight™ 800 (Cat# SA5-10116, Thermofisher). For the appraisal of common painkillers drugs were as follows ...
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bioRxiv - Developmental Biology 2024Quote: ... Complete human medium with 10% exosome depleted FBS (Gibco, Massachusetts, US) was replenished and device placed into incubator ...
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bioRxiv - Immunology 2024Quote: ... and human IL-6 (Gibco, PeproTech, #200-06; 20 ng/mL). Prostaglandin E2 (PGE2 ...
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bioRxiv - Systems Biology 2024Quote: ... Human THP-1 monocytes were cultured in RPMI 1640 medium (Gibco) supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... and cytokine levels were measured using Human Uncoated ELISA Kits (ThermoFisher) targeting IFNψ ...
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bioRxiv - Molecular Biology 2024Quote: Human MET knockout HeLa cells were transiently transfected with Lipofectamine3000 (Invitrogen) according to the manufacturer’s protocols in a 6-well plate format ...
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bioRxiv - Immunology 2024Quote: ... stimulated with Dynabeads™ Human T-Activator CD3/CD28 (Gibco™) and cultured for 5 days in AIMV medium supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Genomics 2024Quote: We used the human Amyloid-β 40 (ThermoFisher Cat No. KHB3481), Amyloid-β 42 (ThermoFisher Cat No ...
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bioRxiv - Plant Biology 2019Quote: ... Aliquots were analyzed on a 10% SDS-PAGE followed by Coomassie Blue staining or Western blot analysis with goat α-6X-His-HRP antibody (Life Technologies, Carlsbad, CA) at a 1:5,000 dilution in Tris-buffered saline (20 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The gene optimized for the Drosophila codon usage and extended by C-terminal His-tag was purchased from Life Technologies (DNA String fragments, USA). The DNA fragment was inserted into a pMT-BiP-V5-His_A vector (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: 1-2 μg of purified 6xHis-tagged calcineurin or 6xHis-MBP as a negative control was first bound to 10 μL magnetic Dynabeads™ His-Tag Isolation and Pulldown Beads (Thermo Fisher Sci., 10104D) in 490 μL base buffer (50 mM Tris-HCl pH 7.5,150 mM NaCl ...
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bioRxiv - Plant Biology 2019Quote: ... 1 μL of each reaction was added to a mix containing 9 μL of Hi-Di Formamide (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and 0.5 μL of GeneScan 500 LIZ Size Standard (Applied Biosystems ...
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bioRxiv - Plant Biology 2019Quote: ... 1 μL of each reaction was added to a mix containing 9 μL of Hi-Di Formamide (Applied Biosystems, Life Technologies, Carlsbad, CA, USA) and 0.5 μL of GeneScan 500 LIZ Size Standard (Applied Biosystems ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by sequencing using the Ion PI™ Hi-Q™ Sequencing 200 Kit on Ion Proton PI™ V2 chips (Thermo Fisher Scientific), an Ion Proton™ System ...
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bioRxiv - Genetics 2020Quote: ... in all the above clonings except for the his-72::GFP repair template which required ccdB resistant bacteria for which we used One Shot ccdB Survival (Thermo Fisher Scientific, cat. A10460). DNA extractions by miniprep were done with the ZymoPURE Plasmid Miniprep kit and elution with water (Zymo Research ...
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bioRxiv - Immunology 2020Quote: ... Nunc MaxiSorp ELISA plates were coated with 50 μL of capture antibody (anti-6x-His antibody clone HIS.H8; 2 μg/mL; Thermo Fisher Scientific, Waltham, MA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2024Quote: ... BMDMs were plated a day in advance on a coverslip lined 24-well plate at 8 x105 cells in 1mL per well, in “BMDM media” supplemented with 10% L929 (20% HI FBS, 1% penicillin-streptomycin, 1% non-essential amino acids (ThermoFisher, cat#11-140-050), 1% sodium pyruvate) ...
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bioRxiv - Microbiology 2024Quote: ... The amplified PCR product of the C-His oipA gene fragment was cloned into vector pLATE11 using aLICator LIC Cloning and Expression Kit 1 (Thermo Fisher Scientific, United States) by following the kit’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Vero/hSLAM and VeroE6/TMPRSS2 were maintained in supplemented DMEM with 10% HI-FBS and 1 mg/mL G418 sulfate (Thermo Fisher 10-131-027). HeLa/ACE2/TMPRSS2 cells were a generous gift from Dr ...
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bioRxiv - Microbiology 2023Quote: ... Protein bands were visualized with Coomassie staining (ROTIBlue Quick, Carl Roth) or immunodetected with a monoclonal anti-His antibody conjugated to HRP (MA1-21315-HRP, Thermo Fisher, 1:2000 diluted). A detailed protocol can be found in protocols.io (dx.doi.org/10.17504/protocols.io.bu67nzhn).
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% (v/v) heat-inactivated (HI) FBS (Thermo Fisher Scientific, Waltham, MA, USA). Stable cell lines expressing F-CIC-S ...
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bioRxiv - Pathology 2023Quote: ... The cDNA was used for RT-qPCR with qPCRBIO SyGreen Blue mix Hi-ROX (PCR Biosystems, London, UK) on the StepOne Plus Real-Time PCR machine (Applied Biosystems, Foster City, CA) and primers listed in Table S2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cDNA plasmids in pcDNA™4/TO Mammalian Expression Vector were transiently transfected into Expi293F™ cells stably expressing human FHF2b and human SCN1B subunit (polyclonal) background using ExpiFectamine™ 293 Transfection Kits (Gibco,Thermo Fisher Scientific CAT #: A14524). Induction was achieved using Tetracycline (Sigma Aldrich) ...
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bioRxiv - Microbiology 2019Quote: ... Eluted proteins were analyzed by SDS-PAGE followed by Coomassie-staining or western blot using an anti-His primary antibody (Thermo Fisher Scientific, Waltham, MA, USA), HRP-conjugated secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Bioengineering 2021Quote: ... THP-1 cells were expanded in RPMI medium (ATCC® 30-2001™) supplemented with 10% heat inactivated fetal bovine serum (HI-FBS, Gibco, ThermoFisher Scientific, Massachusetts, USA), 100 U/ml penicillin and 100 μg/ml streptomycin sulfate (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted protein was then incubated with purified 6x-His-TEV protease E106G (Cabrita et al. 2007) and 500 μM TCEP (Thermo Fisher Scientific, product number 77720) for 20-24 hours ...
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bioRxiv - Microbiology 2021Quote: ... Standard Western blotting procedures were used with the following antibodies: HIV-1 gp120 (NIH AIDS Reagent program catalog no. 288) and 6x-His Tag (ThermoFisher catalog no. MA1-21315-HRP).
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Immunology 2021Quote: ... and SARS-CoV-2 Spike RBD, His, Avitag™ (ACRO Biosystems, SPD-C82E9) immobilized on paramagnetic beads (Dynabeads M-280 streptavidin; Invitrogen Dynal AS, Oslo, Norway) as target antigen ...
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bioRxiv - Cancer Biology 2023Quote: ... we used the modified pSec-NtermHis6 vector containing secretory signal peptide (IgK Leader) as found originally in commercial vector pSecTag/FRT/V5- His-TOPOR vector (Thermo Fisher Scientific, Waltham, MA, USA), but fused to six histidine residues (His6 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed once with 2% HI-FBS S-MEM and further stained with PE-conjugated anti-mouse IgM (Thermo Fisher Scientific, #12-5790-81) for 30 minutes to detect the HTII-280 primary antibody ...
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bioRxiv - Microbiology 2020Quote: Toxicity of the surfaces was tested on human immortalized HaCaT keratinocytes (ThermoFisher). Cells were maintained in DMEM high glucose medium supplemented with Na-pyruvate (1mM) ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T (Human Embryonic Kidney cells 293) cells were obtained from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 293T and human secondary fibroblasts cells were cultured in DMEM (Gibco, 11995) supplemented with 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... in tissue culture plates coated with 2.5μg/cm2 human fibronectin (Thermo Fisher). HEK293T cells and L cells were cultured in DMEM with GlutaMAX supplemented with 10% fetal bovine serum (Omega Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... HCT116 cells (human colorectal epithelial) were maintained using DMEM (Gibco, 41966-029) supplemented with 10% FBS (Sigma ...
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bioRxiv - Immunology 2021Quote: Human interleukin two (IL-2) Ready-SET Go! ELISA kit (eBioscience/Invitrogen) and Nunc MaxiSorp 96-well plates (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... Human iPS WT-11 cells were cultured on Vitronectin (Thermo Fisher Scientific) coated 6-well plates or glass coverslips (for smRNA FISH purposes ...
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bioRxiv - Immunology 2021Quote: ... and Pacific Blue-conjugated mouse anti-human CD274 (ThermoFisher Scientific, MA, USA) mAbs ...
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bioRxiv - Developmental Biology 2020Quote: ... and then incubated in goat anti-human HRP secondary antibody (ThermoFisher Scientific) at 1:10,000 dilution in blocking buffer for 45-60 min at RT ...
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bioRxiv - Immunology 2022Quote: ... and against human CXCL4L1 (rabbit anti-CXCL4L1, PA5-21944, Invitrogen; 1:50). Samples were then prepared for microscopy using Duolink® mounting medium with DAPI ...
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bioRxiv - Biochemistry 2022Quote: ... 100 units/ml human LIF supplemented with 10% fetal bovine serum (Gibco). Cultures were grown in a humidified incubator at 37°C and 5% CO2.
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bioRxiv - Developmental Biology 2022Quote: ... Labelled DNA was then hybridized to Clariom S human GeneChips (ThermoFisher Scientific) and incubated at 45℃ in the GeneChip Hybridization oven 640 (Affymetrix ...
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bioRxiv - Genomics 2019Quote: ... The labeled DNA was then hybridized to Human Gene ST1.0 microarrays (Affymetrix), washed ...
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bioRxiv - Genomics 2020Quote: ... human Malat1 (positive control, Type 1 Probe, VA1-11317, Thermo Fisher Scientific), and bacterial DapB (negative control ...
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bioRxiv - Physiology 2020Quote: ... RNA isolation of human left ventricular biopsies was performed with TRIzol (Invitrogen/ThermoFisher Scientific Inc. ...
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bioRxiv - Cancer Biology 2019Quote: ... anti-human-Alexa 488 or –Alexa 555 (Invitrogen cat #A11013 and #A21433) were used ...
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bioRxiv - Biochemistry 2019Quote: Human cell line HeLa were grown in DMEM (Invitrogen/ThermoFisher Cat #11965) supplemented with 10% FBS and 1X penicillin and streptomycin cocktail ...
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bioRxiv - Biochemistry 2019Quote: Human cell line HeLa were grown in DMEM (Invitrogen/ThermoFisher Cat #11965) supplemented with 10% FBS and 1X penicillin and streptomycin cocktail ...