Labshake search
Citations for Thermo Fisher :
3201 - 3250 of 10000+ citations for Recombinant Human ITGAV & ITGB5 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: ... Chicken anti-rabbit tagged with Alexa Fluor 488 and goat anti-mouse tagged with Alexa Fluor 568 were used as secondary antibody at a dilution of 1:1000 (Invitrogen). Tissues were incubated with these secondary antibodies for 48 hours at 4 °C followed by regular washing ...
-
bioRxiv - Molecular Biology 2020Quote: ... MBP-tagged full-length TRIM21 was cloned into a pFastBac vector for expression in the baculovirus system (Bac-to-Bac, Invitrogen), resulting in pFastBac-MBP-TRIM21 ...
-
bioRxiv - Molecular Biology 2019Quote: Expression constructs for repeat RNAs or GFP-tagged codon-optimized DPRs were transfected in HEK293 cells or U-2 OS cells with Lipofectamine 2000 (Invitrogen) or FuGENE HD (Promega) ...
-
bioRxiv - Molecular Biology 2019Quote: ... with Strep and His epitope tags was amplified by PCR from pIEx10-LRIM1 (Povelones et al. 2009) with BamHI tagged primers (LRIM1-F, LRIM1-R) and subcloned into pJET1.2 (Thermo Scientific) before cloning into pslVg-BGH-attP ...
-
bioRxiv - Molecular Biology 2019Quote: BGH was amplified by PCR from pslVg-GFP-BGH by PCR with BamHI and NotI tagged primers (BGH-F, BGH-R) and subcloned into pJET1.2 (Thermo Scientific). attP was amplified from pBCPB+ (Groth et al ...
-
bioRxiv - Cell Biology 2020Quote: ... Plasmid encoding for FLAG-tagged TM or IM-TM were transfected into EXPI293F cells according to the manufacturer (Thermo Fisher), and incubated at 37°C ...
-
bioRxiv - Neuroscience 2019Quote: ... and subsequently incubated in secondary antibody solution (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) for 1 h at RT ...
-
bioRxiv - Molecular Biology 2019Quote: T7-tagged cDNA template was PCR amplified and subjected to in vitro transcription using a MEGAscript T7 kit (Applied Biosystems). Amplified cRNA was purified with an RNeasy Mini Kit (Qiagen ...
-
bioRxiv - Biochemistry 2020Quote: ... pcDNA5/FRT/TO plasmid encoding Flag-tagged WT ExoU or S142A ExoU was incubated in serum free medium containing lipofectamine 2000 (Invitrogen) for 30 minutes at ambient temperature ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were co-transfected with either a constant concentration of 0.1 μg of NF1-NanoLuc pcDNA expression plasmid or CRAF RBD-NanoLuc pcDNA expression plasmid with 0.2 μg of GFP-tagged KRAS (WT or Mutant) with 0.25 μl of Lipofectamine 2000 per well following the manufacturer’s protocol (Thermo Fisher Scientific). Twenty-four hours later ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were transiently transfected with mCherry-tagged wild type or mutant ORP1L at 70% confluency using Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... The generation of HeLa stable cell lines expressing all different variants of FLAG-tagged HERPUD1 cloned in the pLVX-IRES-Puro vector were generated by transfection with Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... LLC-luc cells were stably transfected with CIITA overexpression vector pcDNA3-myc-tagged CIITA (addgene Plasmid #14650) or control pcDNA3.1 (Thermofisher V79020) using Lipofectamine 2000 Reagent (Invitrogen 11668-027) ...
-
bioRxiv - Biophysics 2020Quote: ... C-terminal His6-tagged CLC-ec1 cloned in the pASK vector was transformed in E.coli BL21(DE3) (Invitrogen, Carlsbad, CA) and the expression of CLC-ec1 protein was induced with anhydrotetracycline (Acros Organics ...
-
bioRxiv - Cell Biology 2022Quote: ... Fluorescently tagged G-actin was prepared by covalent modification with Alexa Fluor™ 594 Carboxylic Acid (Thermo Fisher Scientific 15461054) (Alvarado and Koenderink ...
-
bioRxiv - Molecular Biology 2022Quote: ... from the 2xStrep-tagged or 3xFLAG-tagged plasmids with oligonucleotide primers containing attB recombination sites and recombined into pDONR221 using BP clonase II (ThermoFisher) (Supplementary Table 6) ...
-
bioRxiv - Genetics 2022Quote: ZsGreen and flag-tagged ADAR1-p110 mRNA were made using the Invitrogen MEGAscript T7 Transcription Kit (Fisher Scientific, Cat #: AM1334) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cockayne syndrome B [CSB] fibroblast SV40 immortalized) and CS + CSB (CS cells stably expressing GFP tagged CSB from a BAC) cells were cultured in DMEM (ThermoFisher) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Molecular Biology 2020Quote: Stable cell lines expressing low levels of HA-tagged Gli2 and Gli3 variants were generated using the Flp-In system according to the manufacturer’s recommendations (Life Technologies) (22) ...
-
bioRxiv - Plant Biology 2021Quote: Expression vectors for HA-tagged PpCSLDs under control of their native promoters were constructed using Gateway Multisite Pro cloning (Invitrogen). The CSLD2 and CSLD6 coding sequences were amplified from cDNA clones pdp04669 and pdp21814 (RIKEN BioResource Center ...
-
bioRxiv - Cell Biology 2022Quote: Histidine-tagged Mindin was purified from conditioned media collected from CHO-Mindin cells using Ni-NTA beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2020Quote: ... The resulting TGD057 HA-tagged DNA fragment was then cloned into the pTKO-att parasite expression vector (92) by Gateway Recombination Cloning Technology (Invitrogen). The resulting vector was linearized and transfected into RH Δhxgprt Δku80 parasites by electroporation in a 2 mm cuvette (Bio-Rad Laboratories ...
-
bioRxiv - Biochemistry 2020Quote: ... the clarified lysates from His-tagged or dual tagged constructs were loaded onto an Nickel-nitrilotriacetic acid (Ni2+-NTA; 1.6 mL suspended beads/L growth) column (Thermo Scientific) equilibrated with 10 column volumes (CVs ...
-
bioRxiv - Molecular Biology 2019Quote: ... 6X N-terminally Myc-tagged cDNAs encoding Ku70 or Ku80 WT and mutants were cloned into pcDNA/FRT/TO (Invitrogen) and then transfected along with Flp recombinase expressing pOG44 plasmid using Lipofectamine transfection reagent (Invitrogen ...
-
bioRxiv - Neuroscience 2021Quote: ... Tissue sections were washed thoroughly and then incubated with appropriate secondary antibodies tagged with A lexaFluor-488 or -594 (1:500; Invitrogen) for 1 h at room temperature ...
-
bioRxiv - Cell Biology 2019Quote: ... Stably transfected HEK293 cells expressing N-terminally FLAG-tagged MOR or KOR were cultured in the presence of 250 μg/ml Geneticin (Gibco). For transient DNA expression ...
-
bioRxiv - Biophysics 2020Quote: ... HA or GFP-tagged DISP1 variants (anti-HA rabbit polyclonal, 1:1000 or anti-GFP rabbit polyclonal, 1:2000, Invitrogen), and β-actin (anti-β-actin rabbit polyclonal ...
-
bioRxiv - Neuroscience 2022Quote: ... or isolated human synaptoneurosomes from AD brains (Tai et al., 2014; Tzioras et al., 2019) tagged with pHrodo Red succinimidyl ester (1:20, ThermoFisher) by media change ...
-
bioRxiv - Genomics 2022Quote: A total of 6,114 animals from all year-classes evaluated in the sea lice challenges were pit-tagged and genotyped using custom ThermoFisher (Affymetrix) SNP arrays developed exclusively for AquaGen’s Atlantic salmon populations ...
-
bioRxiv - Microbiology 2022Quote: ... for 1 hour at room temperature followed by additional 1 hour incubation with 1:200 dilution of fluorescence-tagged goat anti-mouse IgG Alexa Fluor 488 (Thermofisher) (Supplemental Table 5) ...
-
bioRxiv - Neuroscience 2022Quote: ... we incubated them in secondary antibody solution (AlexaFluor 488-tagged goat anti-rabbit Ab, 1:1000, ThermoFisher Scientific, A-11008) for 1h at RT ...
-
bioRxiv - Neuroscience 2022Quote: ... and then incubated in Alexa Fluor 647-tagged donkey antirabbit IgG (1:500, Thermo Fisher, catalog # A-31573, RRID: AB_2536183) for 24 hours at 4°C ...
-
bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 647-tagged goat anti-guinea pig antibody (catalog no.A21450, Thermo Fisher Scientific, USA), after which the sections were rinsed ...
-
bioRxiv - Neuroscience 2024Quote: ... blocked with 10 % NDS-PB-TX and incubated in the dark in secondary fluorescently tagged anti-mouse antibody for either TH or GAD1 (1:200, MAB5406, EMD Milipore)) and fluorescently tagged anti-rabbit antibody for tracer (1:200, AlexaFluor, Invitrogen), overnight at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: For expression analysis of truncated WDR47 constructs HEK cells were transfected with different HA tagged Wdr47 constructs using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were transfected with a GFP-tagged plasmid vector expressing spCas9 and gRNA using Lipofectamine 3000 Transfection Reagent following manufacturer’s protocol (ThermoFisher Scientific). Cells were then sorted based on GFP expression after 48 hours from the transfection.
-
bioRxiv - Neuroscience 2024Quote: ... sections were rinsed in PBS and incubated in Alexa Fluor-647-tagged goat anti-mouse IgG (1:100; Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... We generated a strain in which Dnm1 is tagged with mcherry and Mmm1 with GFP (MCY2199) in which mitochondria were labelled with Mitotracker (#M22426, Invitrogen). This setup allowed following and quantifying the presence of Dnm1-mCherry and Mmm1-GFP at sites of mitochondrial fission ...
-
bioRxiv - Cell Biology 2024Quote: ... and transfected with a total of 2 μg C-terminal EGFP tagged M2 tail using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer protocol after washing with 200 μL PBS and refreshing with 100 μL DMEM (ScienCell ...
-
bioRxiv - Biochemistry 2024Quote: ... or expressing EGFP-tagged HRas V12/ HRas V12-C181S/ HRas V12-C184S were lysed in TRIZol at the end of the experiment (Invitrogen). RNA was isolated from the lysate by the chloroform-isopropanol method using the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: The Fab from the CD4i antibody 17b37 was expressed by transient transfection using expression vectors encoding the LC and a C-terminally tagged HC portion of the Fab using the Expi293 expression system (ThermoFisher)7 ...
-
bioRxiv - Immunology 2023Quote: ... followed by incubation with goat anti-rat IgG (H+L) secondary antibody tagged with Alexa Fluor 488 (Invitrogen, A-21470). Tissue sections were washed and blocked with 1:100 mouse Fc block in 5% goat serum ...
-
bioRxiv - Cell Biology 2023Quote: ... Y-A or YIY-AAA) and FLAG-tagged CDK-1 constructs were expressed in FreeStyle 293-F cells (ThermoFisher Scientific). Cells were transfected using FreeStyle MAX Reagent and OptiPRO SFM according to the manufacturer’s guidelines (ThermoFisher Scientific) ...
-
bioRxiv - Neuroscience 2023Quote: ... then rinsed three times for 10 min in 1X PBS and incubated for 4 h at 4°C with the fluorescently tagged secondary antibodies (Invitrogen; AlexaFluor 647 goat anti-mouse and AlexaFluor 488 donkey anti-rabbit ...
-
bioRxiv - Molecular Biology 2023Quote: ... were transfected with expression vectors for 3xFLAG-tagged wt eIF3g or its mutated forms using Expifectamine 293 according to the manufacturer’s protocol (Thermo Scientific). Cells expressing 3xFLAG-tagged eIF3g (200 ml suspension ...
-
bioRxiv - Cell Biology 2023Quote: Exon 1 carrying C-terminally tagged HttQ25-EmGFP and HttQ46-EmGFP were generated in the mammalian expression vector pTREX (Invitrogen). VCP (wt)-EGFP (Addgene ...
-
bioRxiv - Cell Biology 2022Quote: ... Myc or FLAG-tagged procaspase-9 plasmid was prepared by cloning the procaspase-9 gene into a pcDNA-based vector (Invitrogen). Procaspase-9 with three mutations (Asp315Ala ...
-
bioRxiv - Cell Biology 2022Quote: ... and 50 ng Myc-tagged GFP constructs were forward transfected into HFT cells seeded in a 6-well plate using Lipofectamine 3000 (Invitrogen). The cells were harvested 48 or 72 hours post transfection ...
-
bioRxiv - Biochemistry 2023Quote: ... 155-238) were fused to the C-termini of tagged receptors and cloned into the NheI-XhoI sites of pCEP4 (Invitrogen). Plasmids for HIV-1 MN gp160 and BaL gp160 (# 100919 ...
-
bioRxiv - Cell Biology 2023Quote: Lenti-X 293T cells were transfected with pCDNA3.1 plasmids expressing HA-tagged YWHA isoforms using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s protocol and cultured for 48 h before lysis in 2% SDS buffer ...